Operator S Manual Photometer 5010 - V4.4 - RIELE Photometers PDF

Operating Instructions
Photometer 5010
ROBERT RIELE GmbH & Co KG
Software Version 4.4

Documentation Version 10.2004
5010e_44.doc / 02.04.05
SYMBOLS
The packaging material, the type plate on the instrument, and the manual may contain the following symbols or
abbreviations:
Manufactured by:
This product fulfills the requirements of Directive 98/79/EC on

in vitro diagnostic medical devices.
In vitro diagnostic medical device
Caution (refer to accompanying documents)!

Please refer to safety-related notes in the manual accompanying this device.
Please consult instructions for use
Description of technical specifications according to DIN 58 960, part 4
Biohazard
Samples containing material of human origin must be treated as potentially infectious.
The relevant laboratory guidelines on safe use must be observed.
No special protection against penetrating moisture (IP = International Protection)

IP XO
Order number
REF
Serial number
SN
INSTRUMENT APPROVALS
The Photometer 5010 meets the requirement stated in Directive 98/79/EC of the European Parliament and the
Council of the European Union (EU) on in vitro diagnostic medical devices. Furthermore, the Photometer 5010
is manufactured according to the special safety requirements for IVD medical devices stated in DIN EN 61010,
testified by TÜV Rheinland Group.
The Photometer 5010 fulfils the EMC immunity requirements for laboratory use equipment according to the
EMC standard EN 61326.
ROBERT RIELE GmbH & Co KG Page 3 GENERAL NOTES

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SAFETY INFORMATION
User qualification
Only appropriately trained operators are qualified to operate the device.
Environmental conditions
The Photometer 5010 is approved for indoor use only.
For further environmental conditions see chapter 11.1.
Patient ambience
The Photometer 5010 may not be used in the patient ambience.
Electrical Safety
This device was examined and left the factory in perfect technical condition. To preserve this and protect safe
and faultless operation, the user must follow the orders and remarks of this operating manual.
Connect the device to grounded power outlets only. All peripheral devices that are connected to the Photometer
5010 must comply with safety standard EN 60950. Before connecting read the documentation of the peripheral
devices.
Opening covers or removing parts of the instrument, except where this can be achieved manually without the
use of any tool, may expose voltage-carrying components. Connectors can be live, too. Never try to maintain or
repair an open instrument which is carrying voltage.
Repairs at the device including replacement of the Lithium battery may be carried out only by authorized
specialist staff. Through improper repairs the warranty extinguishes, and the user can be heavily jeopardized.
If suspected the device can no longer be operated safely, turn it off and take steps to ensure that no one will
subsequently attempt to use it.
Electromagnetic waves
Devices that emit electromagnetic waves may affect measured data, or cause the Photometer 5010 to
malfunction. Do not operate the following devices in the same room where the Photometer 5010 is installed:
mobile phone, transceiver, cordless phone, and other electrical devices that generate electromagnetic waves.
Reagents
Regarding reagents follow the safety as well as the operating instructions of the manufacturers.
Pay attention to the currently valid German “Gefahrstoffverordnung” (GefStoffV)!
Biological safety
Liquid waste is potentially biologically hazardous. Always wear gloves if handling those materials. Do not touch
parts of the device other than those specified. Consult the laboratory protocol for handling biohazardous
materials.
Pay attention to the currently valid German “Biostoffverordnung” (BioStoffV)!
Spillings and cleaning

If a sample is spilled on the device, wipe up immediately and apply disinfectant.
Waste
Handle liquid waste properly, according to legislation on water pollution, and on the treatment of drainage and
waste matter.

5010e_44.doc / 02.04.05
MANUFACTURER’S WARRANTY
ROBERT RIELE GmbH & Co KG warrants Photometer 5010 against defects in material and workmanship.
For further information contact the local distributor.
WASTE MANAGEMENT NOTE
At the end of the life or utilization time the device and the accessories can be given back to the manufacturer
with costs for an environmental waste disposal. The previous professional decontamination has to be proved
with a certificate.
Address of the manufacturer:

Kurfuerstenstrasse 75-79
D-13467 Berlin
Germany
Phone: +49 (0)30 404 40 87

Fax: +49 (0)30 404 05 29
E-mail: info@riele.de
http://www.riele.de
QUALITY MANAGEMENT SYSTEM
ROBERT RIELE GmbH & Co KG maintains a quality management system according to DIN EN ISO 9001:2000,
and DIN EN ISO 13485:2003 certified by TÜV Rheinland Group.

5010e_44.doc / 02.04.05
CONTENTS
1 INTRODUCTION TO PHOTOMETER 5010 ................................................8

2 INSTALLATION .............................................................................................9
2.1 DELIVERY ........................................................................................................................................ 9
2.2 PREPARATION FOR INSTALLATION.............................................................................................. 9
2.3 INSTALLATION................................................................................................................................. 9
2.4 LOADING PRINTER PAPER ...........................................................................................................10
3 OPERATING ELEMENTS ...........................................................................11

3.1 FRONT.............................................................................................................................................11
3.2 BACK ...............................................................................................................................................11
3.3 WORKING AREA .............................................................................................................................12
3.4 FUNCTIONS OF THE KEYS.........................................................................................................13
3.5 DISPLAY ..........................................................................................................................................14
3.6 KEYBOARD .....................................................................................................................................14
3.7 CUVETTES AND CUVETTE ADAPTOR..........................................................................................14
3.7.1 Changing cuvette adaptor........................................................................................................14
3.7.2 Working with original flow-through system...............................................................................15
3.7.3 Working with single cuvettes....................................................................................................15
3.7.4 Working with discrete flow-through cuvette..............................................................................15
4 SELECTION OF MEASURING PROGRAMS ...........................................16

5 MEASUREMENT WITH BASIC METHODS.................................................17
5.1 GENERAL NOTES..........................................................................................................................17
5.1.1 Fundamental to the handling ... ...............................................................................................17
5.1.2 Fundamental to the heating/cooling ... .....................................................................................17
5.1.3 Fundamental to the inputs ... ...................................................................................................17
5.1.4 Fundamental to the methods with standard ... .........................................................................18
5.1.5 Fundamental to the methods with multi-standards ... ..............................................................18
5.2 MODE FUNCTIONS........................................................................................................................18
5.3 ABBREVIATIONS ............................................................................................................................19
5.4 SURVEY OF THE METHODS .........................................................................................................20
5.5 DESCRIPTION OF METHOD COURSES........................................................................................21
5.5.1 Concentration Measurement with Factor .................................................................................21
5.5.2 Concentration Measurement with Factor and Reagent Blank..................................................21
5.5.3 Concentration Measurement with Factor and Sample Blank ...................................................22
5.5.4 Concentration Measurement with Factor, Sample Blank and Reagent Blank..........................22
5.5.5 Concentration Measurement with Standard.............................................................................23
5.5.6 Concentration Measurement with Standard and Reagent Blank .............................................23
5.5.7 Concentration Measurement with Standard and Sample Blank...............................................24
5.5.8 Concentration Measurement with Standard, Sample Blank and Reagent Blank .....................25
5.5.9 Fixed Time with Factor.............................................................................................................26
5.5.10 Fixed Time with Standard ........................................................................................................27
5.5.11 Kinetic with Factor ...................................................................................................................28
5.5.12 Kinetic with Standard ...............................................................................................................29
5.5.13 Transmission Measurement.....................................................................................................30
5.6 Printer ON / OFF ..............................................................................................................................30
5.7 Pump utilities ....................................................................................................................................30
5.8 Serial interface utilities .....................................................................................................................32
5.9 Changing Sample Numerator ...........................................................................................................33
5.10 Multi-standard tools ..........................................................................................................................33
5.11 Patients data management ..............................................................................................................38
5.11.1 Working with patients data within a course ..............................................................................38
5.11.2 Patients data management utilities ..........................................................................................38
6 USER GUIDANCE AT DISPLAY ...............................................................41

7 METHOD EDITOR.......................................................................................43
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8 UTILITY PROGRAMS .................................................................................47

8.1 SELECTION OF UTILITY PROGRAMS...........................................................................................47
8.2 DESCRIPTION OF UTILITY PROGRAMS.......................................................................................48
8.2.1 Dark level adjustment ..............................................................................................................48
8.2.2 Adjustment of bubble detector .................................................................................................48
8.2.3 Error list ...................................................................................................................................48
8.2.4 Deleting method.......................................................................................................................49
8.2.5 Language selection..................................................................................................................49
8.2.6 Beep ON / OFF........................................................................................................................49
8.2.7 Temperature control ON / OFF ................................................................................................49
8.2.8 Temperature calibration ...........................................................................................................50
8.2.9 AD-Transducer readings..........................................................................................................50
8.2.10 ADC dark current values..........................................................................................................50
8.2.11 Display test ..............................................................................................................................51
8.2.12 Printing device configuration....................................................................................................51
8.2.13 Real time clock ON / OFF ........................................................................................................52
8.2.14 Filter wheel test........................................................................................................................52
8.2.15 Temperature correction single cuvette adaptor........................................................................52
8.2.16 Temperature correction flow-through adaptor..........................................................................53
8.2.17 Printing user defined methods .................................................................................................53
8.2.18 Laboratory name......................................................................................................................54
8.2.19 User name ...............................................................................................................................54
9 MAINTENANCE ...........................................................................................55
9.1 CLEANING INSTRUCTION .............................................................................................................55
9.2 CALIBRATING MEASURING SYSTEM ...........................................................................................55
9.3 ADJUSTMENT OF BUBBLE DETECTOR .......................................................................................55
9.4 CALIBRATION OF PERISTALTIC PUMP ........................................................................................55
9.5 CHANGING RIBBON .......................................................................................................................56
9.6 CHANGING PAPER ROLL ..............................................................................................................56
9.7 CHANGING INLET TUBE ................................................................................................................56
9.8 REPLACEMENT OF LINE FUSES...................................................................................................57
10 ERROR MESSAGE / CORRECTION ........................................................58

10.1 GENERAL NOTE .............................................................................................................................58
10.2 ACOUSTIC ERROR MESSAGES..................................................................................................58
10.3 PLAINTEXT ERROR MESSAGES.................................................................................................58
10.4 CODED ERROR MESSAGES .........................................................................................................58
11 TECHNICAL DATA .....................................................................................61

11.1 ENVIRONMENTAL CONDITIONS ...................................................................................................61
11.2 MINIMAL OPERATION QUALITY ..................................................................................................61
11.3 TYPE PLATE ...................................................................................................................................61
11.4 SHORT SPECIFICATION ................................................................................................................62
11.5 CERTIFICATES ...............................................................................................................................63
12 ACCESSORIES AND SPARE PARTS.........................................................66

13 METHOD LIST ............................................................................................67
13.1 BASIC METHODS............................................................................................................................67
13.2 LIST OF USER SPECIFIC METHODS ............................................................................................68
ROBERT RIELE GmbH & Co KG Page 7 CONTENTS

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1 INTRODUCTION TO PHOTOMETER 5010
This device, Photometer 5010, a programmable photometer for manual applications, is designed for In Vitro
Diagnostic (IVD) use by qualified laboratory staff.
The device is useable e.g. for wet clinical-chemical analyses. The solution can be measured either in disposable
cuvettes placing one after the other or in a flow-through cuvette working with a built-in peristaltic pump. To make
the measuring solution the optimal temperature, use the flow-through cuvette adaptor with built-in cuvette. The
delivered standard cuvette adaptor can be used with a single cuvette.
The solution is quickly and exactly heated or cooled to one of the three possible temperatures (25 °C, 30 °C or
37 °C) by a combination of a power transistor and a Peltier element.
For different measuring methods several programmed methods with open parameters are available (chapter 5 -
MEASUREMENT WITH BASIC METHODS and chapter 13 - METHOD LIST).
13 basic methods with open parameters are available in the device (chapter 5 - MEASUREMENT WITH
BASIC METHODS and chapter 13 - METHOD LIST).
Beside this up to 230 free methods - built up on the basic methods - can be created and stored with a method
editor (chapter 7 - METHOD EDITOR) by the user. The list of methods can be printed out (chapter 8.2.17 -
Printing user defined methods).
Up to 50 nonlinear calibration curves with maximal 20 sets of points can be stored (see polygonal
interpolation or chapter 5.10 - Multi-standard tools).
For the different measuring tasks the device has a capacity for up to 250 pre-programmed test variants with
fixed parameters.
Normally the device has optical filters of 340, 405, 492, 546, 578 and 623 nm wavelength. Two more filters like
e.g. 670 nm can be built in.
Normally the device is operated by a membrane keyboard. External operation is possible by a serial data
interface (chapter 5.8 - Serial interface utilities).
The result is shown on a 32-digit lighted display. The device has a built-in printer and a serial data interface.
According to a GLP conformal documentation the name of lab and user can be printed out as well as transferred
to EDP (chapter 8.2.18 – Laboratory name and 8.2.19 - User name).
Results of patients can be managed in the memory of Photometer 5010 (chapter 5.11 - Patient data
management).
Photometer 5010 supports the download of the operating system using FLASH MEMORY technology. So new
features of software development can easily be updated without any manipulation on hardware level (see
chapter 5.8 -Serial interface utilities: Download to FLASH MEMORY).
In the unlikely case of a fault which cannot be located or remedied by the user, the device can be checked by
built-in utilities for function test and faulty remedy. This guarantees fast, accurate help and easy repair.
For a listing of used terms and text see chapter 5.3 - ABBREVIATIONS.
ROBERT RIELE GmbH & Co KG Page 8 1 - INTRODUCTION TO PHOTOMETER

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2 INSTALLATION
2.1 DELIVERY
After careful unpacking check if the device is in perfect condition. Check that the box contains all the following
items:
1 Aspirate tubing
1 Axis printer paper
1 Dust cover
2 Fuses for line power
1 Mains cable
2 Printer paper
1 Pump tube with joints
1 Ribbon band printer
1 Standard cuvette adaptor
1 Top cover small for printer
1 Waste tubing
Store the packaging carefully for the case of a possibly necessary return. In the event of damage during transit,
contact the local distributor.
2.2 PREPARATION FOR INSTALLATION

The device must be placed on a stable, level surface. The input air at the bottom and the waste air at the back
plate may not be obstructed to guarantee the ventilation of the device.
If the device has been exposed to marked changes in temperature and/or humidity, wait for it to acclimatize
sufficiently before operating.
Before connecting the waste tube to the pump tube remove pump tube at both ends from the metal
clamp. The waste tube of the flow-through system must be led through the tunnel to the backside of the device
(chapter 3.2 - BACK). Provide a drain tank, and lead the tip of waste tube into the drain tank.
2.3 INSTALLATION
The Photometer 5010 operates at any line voltage between 90 VAC and 264 VAC at 50/60 Hz without adjustment.
The device plug of the mains cable must be put into the mains cable socket at the back of the device and the
mains plug of the mains cable must be put into a grounded mains socket.
For combined operation with external devices (PC, printer) read the relating documentation. Pay atten-
tion to the above safety instructions. Switch these devices on at first.
Switch the photometer on by pressing the mains switch at the back of the device.
(c) ROBERT RIELE After switching on Copyright, ...

GMBH & CO KG
(c) ROBERT RIELE Website, ...

www.riele.de
PHOTOMETER 5010 ... Type of device and version number is displayed. Parallel to this, type of device
V4.xa dd.mm.yy D and version number is printed in case of an activated printer.
TEMP. 37C <> Display in case of an activated temperature control. It disappears after reaching
PLEASE WAIT +B of selected temperature.
Select different temperature with [+].
Proceeding to next display with [BREAK] is possible.
ROBERT RIELE GmbH & Co KG Page 9 2 - INSTALLATION

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METHOD NO. After this the level for program selection appears.
INPUT 1-250
The program selection is carried out in accordance with chapter 4 - SELECTION
OF MEASURING PROGRAMS
The device is heated up and ready for measurement after around 15 minutes. At first the heating/cooling is
switched off. If the user wishes to work later with tempered measuring solution, it is recommended to switch on
the heating/cooling now to stabilize itself parallel to the warming up of the device. Switch on the heating/cooling
directly by utility program (chapter 8.2.7 -Temperature control ON / OFF) or indirectly by selection of a method
with programmed warming up (for details see chapter 5.1 - GENERAL NOTES).
If errors appear during operation, first of all they have to be confirmed with [ENTER] before remedying (see
chapter 10 - ERROR MESSAGE / CORRECTION).
If required, a dark level adjustment can also be executed at this time according to chapter 8.2.1 - Dark level
adjustment.
2.4 LOADING PRINTER PAPER

At the first putting into operation or when the end of printer paper is color marked, new printer paper must be
inserted:
• Open the printer cover and remove the rest of paper.
• Put printer paper axis into the new printer paper reel.
• Fold the beginning of paper over the corner to an angle of 90° (as shown below).
• Put this tip into the guide below the ribbon cassette.
• Press [LF] several times for line-feed until the paper has passed the tear-off edge.
• Put printer paper reel into the axis guide.
• Take paper through the crack of the printer cover and close the printer with the cover.
ROBERT RIELE GmbH & Co KG Page 10 2 - INSTALLATION

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3 OPERATING ELEMENTS
3.1 FRONT
display printer cover

lid
working area
keyboard
sipping lever [P]
aspirate tubing
3.2 BACK
ROBERT RIELE GmbH & Co KG Page 11 3 - OPERATING ELEMENTS

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3.3 WORKING AREA
waste tubing pump tube with joints

REF 1704834001 REF 5010-013
outlet tube cuvette

REF 5010-015
aspirate tubing
REF 5010-014
joint inlet tube cuvette

REF 1707175001
waste tubing
REF 1704834001
aspirate tubing
REF 5010-014
joint inlet tube cuvette pump tube with joints

REF 1707175001 REF 5010-013
outlet tube cuvette

REF 5010-015

5010e_44.doc / 02.04.05
3.4 FUNCTIONS OF THE KEYS
NORMAL OPERATION
Key/-combination Function Chapter
[0]...[9], [•] Keys for numeric input 5.5/ 7/ 8

[MODE] MODE function for alterations of adjustment 5.2 / 8
[LF] Line feed of paper 2.4
[+], [-] Sign change
Choice keys for methods, MODE functions and utilities 4/7/8
Detailed printout after carried out Kinetic measuring
[BREAK] General abort function 4/7/8
[ZERO] Setting to zero 5.1/ 5.5
[ENTER] Confirmation key 5.1/ 5.5
[RESULT] Triggering measuring 5.1/ 5.5
[WASH] Starts the suction pump for the programmed time 5.1
[P] Flow-through cuvette: sipping lever with further functions 5.1
MODE FUNCTIONS
[MODE] [4] Printer ON / OFF 5.6

[MODE] [5] Multi-standard tools 5.10
[MODE] [6] Pump utilities 5.7
[MODE] [7] Serial interface utilities 5.8
[MODE] [8] Changing Sample Numerator 5.9
[MODE] [9] METHOD EDITOR 7
[MODE] [MODE] UTILITY PROGRAMS 8
[MODE] [LF] SERVICE TOOLS Service
manual
TEXT EDITOR LEVEL

[7 / ABCD] Input of A...B...C...D 7

[8 / EFGH] Input of E...F...G...H 7
[9 / IJKL] Input of I...J...K...L 7
[4 / MNOP] Input of M...N...O...P 7
[5 / QRST] Input of Q...R...S...T 7
[6 / UVWX] Input of U...V...W...X 7
[1 / YZ12] Input of Y...Z...1...2 7
[2 / 3456] Input of 3...4...5...6 7
[3 / 7890] Input of 7...8...9...0 7
[0 / / # % -] Input of /...#...%...- 7
[WASH / A↔a] Change between capital and small characters 7
[• / Chr.→] Change of the cursor to the next input position 7

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3.5 DISPLAY
3.6 KEYBOARD
3.7 CUVETTES AND CUVETTE ADAPTOR
3.7.1 Changing cuvette adaptor

Switch off the instrument! Remove milled screw and take out the adaptor. Put in the other one. Pay attention to
intact contact pins and absolute cleanliness of lens and adaptor underside. The adaptor can easily be put in.
Move milled screw only by hand!
If change from flow-through cuvette to disposable cuvette is wanted, it is absolutely necessary to empty tube
system by pressing [WASH] followed by pressing sipping lever [P] several times without sipping solution before
removing tube connections.
After replacing of a cuvette adaptor by a new one a dark level adjustment should be done after reaching
operating temperature (see chapter 8.2.1 - Dark level adjustment).

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3.7.2 Working with original flow-through system

Insert plug of the bubble detector to its connector at the instrument. The bubble detector has to sit directly on
the flow-through cuvette. If operation without bubble detector was required, switch it OFF, and do a pump
calibration (see chapter 5.7 - Pump utilities).
Lead inlet tube forward through the metallic one. Fasten outlet tube cuvette to metal joint of cuvette as well as of
pump tube.
Before connecting the tubes remove pump tube at both ends from the metal clamp. The pump tube
should not be clamped during longer periods of inactivity:
pump tube with joints

in not clamped situation
While working with the flow-through system the tubes may not be strongly bended. There should be no
residues inside. Check from time to time that the tubing and the connections are leakproof. Do a pump
calibration (chapter 5.7 - Pump utilities) after each changing of tubes.
Before as well as within a series of measuring, and after all measuring it is absolutely necessary to wash the
tube system repeatedly with distilled water or another appropriate rinse solution by pressing [WASH] and
sipping lever [P]. This is also necessary after having changed the method (chapter 9.1 - CLEANING
INSTRUCTION). The washing process needs 1500 µl solution.
Press [WASH], then sip rinse solution or next sample by pushing sipping lever [P].
To sip solution into the measuring system, put inlet tube deep enough into the respective vessel.
For setting to zero press [ZERO] and push sipping lever [P].
Measuring of solutions is done by pushing sipping lever [P].
For repeated measuring of sipped solutions press [RESULT].
3.7.3 Working with single cuvettes

Use the single cuvette adaptor. Switch OFF pump (see chapter 5.7 - Pump utilities). Put in single cuvette. Pay
attention to the path of rays from right to left (see drawing OPTIC CONSTRUCTION in TECHNICAL DATA).
Measuring is generally triggered by [RESULT], zero measuring by [ZERO].
3.7.4 Working with discrete flow-through cuvette

Use the single cuvette adaptor. Put in flow-through cuvette. Pay attention to the path of rays from right to left
(see drawing OPTIC CONSTRUCTION in TECHNICAL DATA). Pay attention to correct connection of aspirate
as well as outlet tubing. Switch ON pump, switch ON or OFF bubble detector, and do a pump calibration (see
chapter 5.7 - Pump utilities).

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4 SELECTION OF MEASURING PROGRAMS
METHOD NO. A method can be called directly in the level for program selection by input of the
INPUT 1-250 method number (see also chapter 2.3 - INSTALLATION and chapter 13 -
METHOD LIST). Confirm with [ENTER].
It can also be selected indirectly by repeated pushing of [+/-], so that the methods
are scrolled till the required method is reached.
No. Program group Chapter Description
1 ... 250 Direct method selection 5 MEASUREMENT WITH BASIC METHODS

6 USER GUIDANCE AT DISPLAY
13 METHOD LIST
[MODE] Utilities 8 UTILITY PROGRAMS

[MODE]
A combination of direct and indirect selection of a method is possible.
[BREAK] has a general abort function for all methods and subroutines back to the level for program selection.
ROBERT RIELE GmbH & Co KG Page 16 4 - SELECTION OF MEASURING PROGRAMS

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5 MEASUREMENT WITH BASIC METHODS
5.1 GENERAL NOTES

The device offers user guidance in the display by a combination of plaintext and short terms (chapter 6 - USER
GUIDANCE AT DISPLAY). If not already automated, e.g. at selection of a basic method, the demanded action
is displayed, and parameters are queried.
In principle, reports and inputs to the method have to be confirmed with [ENTER]. With [BREAK] all methods
can be broken off. New start according to chapter 4 - SELECTION OF MEASURING PROGRAMS. Measuring
is generally triggered by sipping lever [P] or [RESULT], zero measuring by [ZERO] and sipping lever [P]
(see 3.7.3 - Working with single cuvettes and 3.7.2 - Working with original flow-through system).
5.1.1 Fundamental to the handling ...

• The lid over cuvette compartment has to be closed before all measuring with single cuvettes.
• Deviations from normal operation, caused by the device or by the user, e.g. the programmed
upper limit was exceeded by the measurement, are notified by “ERROR”. They have always to be
confirmed with [ENTER] (chapter 10 - ERROR MESSAGE / CORRECTION).
5.1.2 Fundamental to the heating/cooling ...

• The switching state of the heating/cooling is parameter of a method. Therefore switching ON or OFF of
the heating/cooling is carried out via selection of a method.
• The heating/cooling can be switched over manually.
TEMP. 37C <> Display in case of an activated temperature control. It disappears after
PLEASE WAIT +B reaching selected temperature.
Select different temperature with [+].
Proceeding to next display with [BREAK] is possible.
The heating/cooling can also be switched over manually by utility program 7 (chapter 8.2.7).
• After switching on the heating/cooling it lasts around 5 minutes till a constant temperature of 25 °C,
30 °C or 37 °C is reached.
• Indicator for the heating/cooling process, and so for a not yet constant temperature, is a flashing cursor
at the utmost right position in the upper display line and an asterisk (*) at the utmost right position in
the corresponding print line.
5.1.3 Fundamental to the inputs ...

• The entry format of the factor or the standard with sign determines the output format of the result
regarding the number of the fractional digits.
• Instead of being measured, the reagent blank can also be entered via the keyboard or put on zero.
• In favor of a homogeneous solution the input of a delay before a measuring is possible at all methods.
ROBERT RIELE GmbH & Co KG Page 17 5 - MEASUREMENT WITH BASIC METHODS

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5.1.4 Fundamental to the methods with standard ...

• Each measurement of a standard (calibrator) can be performed as single, double or triple
determination.
After measurement of a standard with e.g. triple determination following is displayed:
0.302 0.299 The first line shows the absorbance 1 and 2 of the standard.
0.303 E/+/0 The second line shows the absorbance 3 of the standard.
With [ENTER] the average of all values is calculated. Values with 0 are
ignored and excluded from the calculation. The resulting factor is
calculated on the average of the standard.
With [+] a selection between the values is done. A black rectangle

marks the actual value (in this case 0.299).
With [0] a value is deleted. An existing value will be eliminated.
With [RESULT] a measurement is performed.
• The determined resulting factor of a standard measuring is saved together with the corresponding
method number. After renewed selection of a method number the resulting factor is offered as
„Old STD“.
5.1.5 Fundamental to the methods with multi-standards ...

• Linear calibration is used in the case of two calibrators. The assay absorbance at different
concentrations forms a linear plot (chapter 5.10 - Multi-standard tools).
• Nonlinear calibration is used for those sample constituents where assay absorbance at different
concentrations forms a nonlinear but reproducible plot. At least three (maximum 20) calibrators are
required for nonlinear calibration (chapter 5.10 - Multi-standard tools).
5.2 MODE FUNCTIONS

The mode functions serve for changing definite basic settings during a course of method. It is not necessary to
exit the method and to change to the level of UTILITY PROGRAMS (chapter 8). Also temporary parameters
remain unchanged. Corresponding to these features this group of functions is available only within the repetition
sequence of a course of method (chapter 5.5 - DESCRIPTION OF METHOD COURSES).
Change to the selection level of the mode functions with [MODE].
MODE FUNCTIONS Selection level of the mode functions: Selecting the desired mode function by
direct selection i.e. input of the number key in question (see table) or by scrolling
through all mode functions with [+] ... [+]. Confirm with [ENTER].
Exit the selection level of the mode functions and change back to the course of
method with [BREAK].
After working off a mode function the return is carried out to the course of
method.
MODE FUNCTIONS
[MODE] [4] Printer ON / OFF 5.6
[MODE] [5] Multi-standard tools 5.10
[MODE] [6] Pump utilities 5.7
[MODE] [7] Serial interface utilities 5.8
[MODE] [8] Changing Sample Numerator 5.9
ROBERT RIELE GmbH & Co KG Page 18 5 - MEASUREMENT

ASUREMENT WITH BASIC METHODS
5010e_44.doc / 02.04.05
5.3 ABBREVIATIONS
A, ABS ................................ Absorbance

ARB ...................................... Absorbance of reagent blank
ARB,0 .................................... At Fixed Time: Absorbance of reagent blank after incubation time T0
ARB,1 .................................... At Fixed Time: Absorbance of reagent blank after reaction time T1
ARBB ..................................... Absorbance of blank of reagent blank
AS ........................................ Absorbance of sample
AS,0 ...................................... At Fixed Time: Absorbance of sample after incubation time T0
AS,1 ...................................... At Fixed Time: Absorbance of sample after reaction time T1
AS,0 ... AS,5 ........................... At Kinetic: Initial absorbance and 1st ... 5th absorbance of sample
ASB ...................................... Absorbance of sample blank
AST....................................... Absorbance of standard
AST,0..................................... At Fixed Time: Absorbance of standard after incubation time T0
AST,1..................................... At Fixed Time: Absorbance of standard after reaction time T1
ASTB ..................................... Absorbance of standard blank
C ......................................... Concentration
CST ...................................... Concentration of standard
∆ARB,Minute ............................ At Kinetic: Change of reagent blank per minute (measured in ∆A / min)
∆AS,Minute .............................. At Kinetic: Change of sample per minute (measured in ∆A / min)
F.......................................... Factor
FTK ..................................... Fixed Time Kinetic
KIN ...................................... Kinetic
nm ....................................... Nanometer (dimension of wavelength)
R ......................................... Result, Sample

Rb ....................................... Reagent blank
Rbb ..................................... Blank of Reagent blank
R^2...................................... At Kinetic: Square of correlation coefficient shows the linearity of a test
S, ST ................................... Standard
STb ..................................... Standard blank
Sb........................................ Sample blank
TRANSM., T........................ Transmission in %

T0 ........................................ At Fixed Time: Incubation time in seconds
T1 ........................................ At Fixed Time: Reaction time in seconds
T1 ........................................ At Kinetic: Time per delta in seconds

5.4
The calculation procedures, on which all methods are traceable from the list of methods, are mentioned in the following table. Criterion is the characteristic
of the calculation procedure (see below). For detailed description of the respectively accompanying course of method see chapter 5.5 - DESCRIPTION OF METHOD
COURSES
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CP-No. *) Description*) Characteristic*) Method Calculation formula*) Factor RB

CP 1 chapter 5.5.1 C/F End Point with Factor C = F ∗ AS given / entering --
CP 2 chapter 5.5.2 C/F/Rb End Point with Factor C = F ∗ (AS - ARB) given / entering ent./meas.

CP 3 chapter 5.5.3 C/F/Sb End Point with Factor C = F ∗ |AS - ASB| given / entering --
SURVEY OF THE METHODS
CP 4 chapter 5.5.4 C/F/SbRb End Point with Factor C = F ∗ (|AS - ASB| - |ARB - ARBB|) given / entering ent./meas.
CP 5 chapter 5.5.5 C/S End Point with Standard C = F ∗ AS F = CST / AST --
CP 6 chapter 5.5.6 C/S/Rb End Point with Standard C = F ∗ (AS - ARB) F = CST / (AST-ARB) ent./meas.
CP 7 chapter 5.5.7 C/S/Sb End Point with Standard C = F ∗ |AS - ASB| F = CST / |AST-ASTB| --
CP 8 chapter 5.5.8 C/S/SbRb End Point with Standard C = F ∗ (|AS - ASB| - |ARB - ARBB|) F = CST / ( |AST-ASTB| - |ARB-ARBB| ) ent./meas.
Page 20
CP 9 chapter 5.5.9 FTK/F/Rb Fixed Time with Factor C = F ∗ ( |AS,0 - AS,1| - |ARB,0 - ARB,1| ) given / entering ent./meas.
CP 10 chapter 5.5.10 FTK/S/Rb Fixed Time with Standard C = F ∗ ( |AS,0 - AS,1| - |ARB,0 - ARB,1| ) F = CST / (|AST,0-AST,1|-|ARB,0-ARB,1|) ent./meas.
CP 11 chapter 5.5.11 KIN/F/Rb Kinetic with Factor C = F ∗ ( ∆AS,Minute - ∆ARB,Minute ) given / entering ent./meas.
CP 12 chapter 5.5.12 KIN/S/Rb Kinetic with Standard C = F ∗ ( ∆AS,Minute - ∆ARB,Minute ) F = CST / ( ∆AST,Minute - ∆ARB,Minute ) ent./meas.
CP 13 chapter 5.5.13 TRANSM. Transmission in %
*) Explanations:
CP-No. ................................................. Number of the calculation procedure (see also chapter 7 - METHOD EDITOR)
Description ........................................... Refer to the description of the corresponding method course
Characteristic ....................................... Characteristic of the calculation procedure (see also chapter 7 - METHOD EDITOR and chapter 13 - METHOD LIST)
Calculation formula .............................. Calculation basis of basic method, abbreviations and formula sign (chapter 5.3 - ABBREVIATIONS)
Rb ........................................................ Reagent blank
ent./meas.. ........................................... Reagent blank can be entered or measured
5 - MEASUREMENT WITH BASIC METHODS

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5.5 DESCRIPTION OF METHOD COURSES
At first the device runs through preparatory measuring till a sequence starts which recurs for every further test.
5.5.1 Concentration Measurement with Factor

Method at which a measured sample value AS is multiplied with a predefined factor F.
Calculation procedure .......................................................CP 1

Characteristic ....................................................................C / F
Method ....................................................End Point with Factor
Calculation formula .................................................. C = F ∗ AS
Factor............................................................... given / entering
Course representation (preprogramable parameters are marked with pre-programmable)

Selection of wavelength pre-programmable
Input of factor pre-programmable
Selection of volume pre-programmable
Selection of temperature pre-programmable
Input of delay pre-programmable
Bringing in and measuring zero solution
---------------------------------------------------------------------------------- Start of repetition sequence ----------------------------
Bringing in sample
Measuring sample and output of result
---------------------------------------------------------------------------------- End of repetition sequence -----------------------------
5.5.2 Concentration Measurement with Factor and Reagent Blank

Method at which the difference of sample value AS and reagent blank ARB is multiplied with a given factor F.
The reagent blank ARB is entered or measured once.

Characteristic ............................................................ C / F / Rb
Calculation formula .......................................C = F ∗ (AS - ARB)
Reagent blank ........................................ entering or measuring
Course representation (pre-programmable parameters are marked with pre-programmable)

Bringing in and measuring reagent blank (Rb)
or: Input of reagent blank (Rb) in absorbance
Bringing in sample

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5.5.3 Concentration Measurement with Factor and Sample Blank

Method at which the difference of sample value AS and sample blank ASB regarding the amount is multiplied with
a given factor F. The sample blank ASB is measured before every test.

Characteristic ............................................................ C / F / Sb
Calculation formula ....................................... C = F ∗ |AS - ASB|

Bringing in and measuring sample blank (Sb)
Measuring sample blank (Sb)
Bringing in and measuring sample
5.5.4 Concentration Measurement with Factor, Sample Blank and Reagent Blank
Method at which the difference of reagent blank ARB and blank of reagent blank ARBB regarding the amount is
subtracted of the difference of sample value AS and sample blank ASB regarding the amount, and this difference
is multiplied with a given factor F.
The sample blank ASB is measured before every test. The reagent blank ARB is entered or measured once.

Characteristic ........................................................C / F / SbRb
Calculation formula .............. C = F ∗ ( |AS - ASB| - |ARB - ARBB| )

Bringing in blank of reagent blank (Rbb)
Measuring blank of reagent blank (Rbb)
Bringing in reagent blank (Rb)
Measuring reagent blank (Rb)
or: Input of difference Rb-Rbb in absorbance
Bringing in sample blank (Sb)
Bringing in sample

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5.5.5 Concentration Measurement with Standard

Method at which a measured absorbance value AS is multiplied with a factor F which is determined by
measuring of a standard solution with known concentration CST.

Characteristic ....................................................................C / S
Method ............................................... End Point with Standard
Calculation formula .................................................. C = F ∗ AS
Resulting factor .................................................... F = CST / AST

Input of standard concentration CST pre-programmable
Bringing in standard solution (ST)
Measuring standard solution (ST) and output of resulting factor
Confirming factor with [ENTER] or abort with [BREAK]
Bringing in sample
5.5.6 Concentration Measurement with Standard and Reagent Blank

Method at which the difference of sample value AS and reagent blank ARB is multiplied with a factor F which is
determined by measuring of a standard solution with known concentration CST and under consideration of
reagent blank ARB.
The reagent blank ARB is entered or measured once.

Characteristic ............................................................ C / S / Rb
Calculation formula .......................................C = F ∗ (AS - ARB)
Resulting factor ........................................... F = CST / (AST-ARB)

or: Input of reagent blank (Rb) in absorbance
Bringing in sample

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5.5.7 Concentration Measurement with Standard and Sample Blank

Method at which the difference of sample value AS and sample blank ASB regarding the amount is multiplied with
a factor F which is determined by measuring of a standard solution with known concentration CST and under
consideration of standard blank ASTB.
The sample blank ASB is measured before every test.

Characteristic ............................................................ C / S / Sb
Calculation formula ....................................... C = F ∗ |AS - ASB|
Resulting factor ........................................ F = CST / |AST - ASTB|

Bringing in standard blank (STb)
Measuring standard blank (STb)
Bringing in sample

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5.5.8 Concentration Measurement with Standard, Sample Blank and Reagent Blank
Method at which the difference of reagent blank ARB and blank of reagent blank ARBB regarding the amount is
subtracted of the difference of sample value AS and sample blank ASB regarding the amount and this difference
is multiplied with a factor F which is determined by measuring of a standard solution with known concentration
CST and under consideration of standard blank ASTB and the difference of reagent blank ARB and blank of
reagent blank ARBB
The sample blank ASB is measured before every test. The reagent blank ARB is entered or measured once.

Characteristic ........................................................C / S / SbRb
Calculation formula .............. C = F ∗ ( |AS - ASB| - |ARB - ARBB| )
Resulting factor ...................F = CST / ( |AST-ASTB| - |ARB-ARBB| )

Bringing in blank of reagent blank (Rbb)
Measuring blank of reagent blank (Rbb)
or: Input of difference Rb-Rbb in absorbance
Bringing in standard blank (STb)
Measuring standard blank (STb)
Bringing in sample

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5.5.9 Fixed Time with Factor

Method at which is measured a reagent blank after an incubation time (⇒ ARB,0) and after a reaction time (⇒
ARB,1) and also a sample after an incubation time (⇒ AS,0) and after a reaction time (⇒ AS,1).
The difference from the change of the test and the change of the reagent blank is multiplied by a predefined
factor F. The reagent blank ARB is entered or measured once.
While performing the course the dialog asks for the use of a reagent blank. The default value is OFF. To
continue without reagent blank press [ENTER].

Characteristic ........................................................ FTK / F / Rb
Method ................................................. Fixed Time with Factor
Calculation formula ......... C = F ∗ ( |AS,0 - AS,1| - |ARB,0 - ARB,1| )

Input of incubation time pre-programmable
Input of reaction time pre-programmable
Bringing in and measuring reagent blank (Rb) or: Input of difference of reagent blanks |ARB,0-ARB,1| in
absorbance
Bringing in sample
• Confirming result and changing to next sample with [ENTER]
• or: Further measuring of AS,1 with [RESULT]
• or: Bringing in zero solution and measuring zero with [ZERO]
• or: Abort with [BREAK]
Abbreviations at printout:
Rb[A] Initial value of Rb in absorbance
DELTA ABS Difference between both measurements in absorbance
Column „ABS.“ Initial value of Kinetic in absorbance
Column „RESULT“ Result of Kinetic under consideration of Rb

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5.5.10 Fixed Time with Standard

Method at which is measured a reagent blank after an incubation time (⇒ ARB,0) and after a reaction time (⇒
ARB,1) and also a sample after an incubation time (⇒ AS,0) and after a reaction time (⇒ AS,1).
The difference from the change of the sample and the change of the reagent blank becomes multiplied with a
factor F which is determined by means of the change of standard solution |AST,0-AST,1| and the change of
reagent blank |ARB,0-ARB,1| during the reaction time and given concentration of standard. The reagent blank ARB
is entered or measured once.
Calculation procedure .....................................................CP 10

Characteristic ........................................................FTK / S / Rb
Method ............................................. Fixed Time with Standard
Calculation formula ......... C = F ∗ ( |AS,0 - AS,1| - |ARB,0 - ARB,1| )
Resulting factor ..............F = CST / ( |AST,0-AST,1| - |ARB,0-ARB,1| )

Input of incubation time pre-programmable
Input of reaction time pre-programmable
or: Input of difference of the reagent blanks |ARB,0-ARB,1| in absorbance
Bringing in sample
• or: Further measuring of AS,1 with [RESULT]
DELTA ABS Difference between both measurements in absorbance
ST[A] Initial value of standard in absorbance

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5.5.11 Kinetic with Factor

Method at which a sample S is measured several times (depending on the number of deltas) in an equidistant
time grid. From the resulting absorbance values an alteration per minute ∆AS,Minute is determined by a regression
calculation. The reagent blank ∆ARB,Minute is measured in the same way as the sample (or entered directly in U/l)
and subtracted from the sample value. This difference is multiplied by a given factor F.

Characteristic .........................................................KIN / F / Rb
Method ........................................................ Kinetic with Factor
Calculation formula .................C = F ∗ ( ∆AS,Minute - ∆ARB,Minute )
Number of deltas........................................... entering (3 to 28)
Time per delta ........................................entering (4 s to 255 s)

st
Input of delay before 1 measuring pre-programmable
Input of number of deltas pre-programmable
Input of time per delta pre-programmable
Output of result of reagent blank in U/l and of correlation coefficient R^2 - confirming with [ENTER]
or: Input of reagent blank (Rb) in U/l and confirming with [ENTER]
Bringing in sample
optional with [+] detailed print-out of U/l in each measuring interval
• or: Measuring same sample with [RESULT]
DELTA ABS Result in ∆A / min, generated from the first up to the last measurement in absorbance
DELTA REG Result in ∆A / min, generated from a regression calculation
R^2 Square of correlation coefficient shows the linearity of the test

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5.5.12 Kinetic with Standard

Method at which a sample S is measured several times (depending on the number of deltas) in an equidistant
time grid. From the resulting absorbance values an alteration per minute ∆AS,Minute is determined by a regression
calculation. The reagent blank ∆ARB,Minute is measured in the same way as the sample (or entered directly in U/l)
and subtracted from the sample value. This difference is multiplied by a factor F which is determined by
measuring of a standard solution ∆AST,Minute with known concentration CST and under consideration of the
reagent blank ∆ARB,Minute.

Characteristic .........................................................KIN / S / Rb
Method .................................................... Kinetic with Standard
Calculation formula .................C = F ∗ ( ∆AS,Minute - ∆ARB,Minute )
Resulting factor ...................F = CST / ( ∆AST,Minute - ∆ARB,Minute )
Number of deltas........................................... entering (3 to 28)
Time per delta ........................................entering (4 s to 255 s)

st
Input of delay before 1 measuring pre-programmable
Input of number of deltas pre-programmable
Input of time per delta pre-programmable
Output of result of reagent blank ∆ARB,Minute in U/l and of correlation coefficient R^2 - confirming with [ENTER]
or: Input of reagent blank (Rb) in U/l and confirming with [ENTER]
Bringing in sample
optional with [+] detailed print-out of U/l in each measuring interval
• or: Measuring same sample with [RESULT]
DELTA ABS Result in ∆A / min, generated from the first up to the last measurement in absorbance
DELTA REG Result in ∆A / min, generated from a regression calculation
R^2 Square of correlation coefficient shows the linearity of the test
ST/KIN 1 … ST/KIN 3 Result of standard 1 ... 3 in ∆A / min

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5.5.13 Transmission Measurement

Characteristic ................................................................. T in %

Bringing in sample
5.6 Printer ON / OFF

Start menu by [MODE] [PARALLEL]:
The internal printer can be activated or deactivated.
PRINTER ON The present status of the printer is displayed in the first line.
1/ON 0/OFF Activate printer by [1] or deactivate by [0].
With [BREAK] the setting is taken temporarily up to next switching on.
With [ENTER] the status is stored.
5.7 Pump utilities

Start menu by [MODE] [PUMP]:
• Item [1]: Perform a pump calibration

MENU PUMP 1-4/+
• Item [2]: Pump ON / OFF
• Item [3]: Pump reverse
• Item [4]: Bubble detector ON/OFF
Select item.
Item 1: Pump calibration

PUMP CALIBR. The procedure supports working with and without bubble detector. Activate (ON)
NEXT KEY E/B/+ or deactivate (OFF) the bubble detector first by item [4].
Confirm with [ENTER].

The pump drains the tube system. Do not sip any solution!
--------------------------- In case of working with bubble detector ---------------------------

PUMP CALIBR. Measure exactly 1000 µl distilled water into a sample cup. Then sip distilled water
1000 ul -> P by pushing sipping lever [P]. The sipping volume of 1000 µl is automatically
measured in the flow-through cuvette.
PUMP CALIBR. The length of transported air (e.g. A 139) and water (e.g. W 1204) are displayed.
A 139 W 1204 Finish calibration by pressing [ENTER].
Attention: If calibration is impossible, although the peristaltic pump tube is

clamped and 1000 µl are sipped, do the media test (see to chapter 8.2.2 -
Adjustment of bubble detector).
Then start again with pump calibration.

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------------------------ In case of working without bubble detector -------------------------

A W Measure exactly 1000 µl distilled water into a sample cup.
1000 ul -> P
A 39 W Then sip distilled water by pushing sipping lever [P] repeatedly. With each push
MS AIR P/E/B the pump motor turns a little bit. The turn is shown as e.g. 39 in the first line of
display. During this phase the system is pumping air. Stop with further turn when
the liquid is seen in the small part of tube between metal tube inlet and joint inlet
tube cuvette. Confirm with [ENTER].
A 141 W During the second phase the system pumps the volume of 1000 µl distilled water.
MS WATER P/E/B Push sipping lever [P] repeatedly.
A 141 W 279 After each step the turn is shown as e.g. 279 in the first line of display. Stop with
MS WATER P/E/B further turn when the end of the liquid is seen in the small part of tube between
metal tube inlet and joint inlet tube cuvette.
A 141 W 1189 The length of transported air (e.g. A 141) and water (e.g. W 1189) are displayed.
MS WATER P/E/B Finish calibration by pressing [ENTER].
Item 2: Pump ON / OFF

PUMP ON/OFF Confirm with [ENTER].
NEXT KEY E/B/+
PUMP ON The present status of the pump is displayed in the first line.
1/ON 0/OFF Activate pump by [1] or deactivate by [0].
Item 3: Pump reverse

PUMP REVERSE Put a sample cup at the aspiration tube. By pushing sipping lever [P] the pump
PUMP -> P turns in the reverse direction. The sipped liquid is transferred back into the
sample cup.
Item 4: Bubble detector ON/OFF

B-DET. ON/OFF Confirm with [ENTER].
NEXT KEY E/B/+
B-DET. ON The present status of the bubble detector is displayed in the first line.
1/ON 0/OFF Activate bubble detector by [1] or deactivate by [0].

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5.8 Serial interface utilities

At the rear, the Photometer 5010 has an RS 232 serial interface through which it can be connected to a PC or
an external printer. A suitable data cable can be supplied (REF 501-002). The connected device must comply
with safety standard EN 60950.
Start menu by [MODE] [SERIAL]:
The interface for serial inputs / outputs can be activated or deactivated
MENU SER. 1-5/+ • Item [1]: Activate or deactivate external printer with serial interface by [1]
• Item [2]: Activate or deactivate EDP by [2]
• Item [3]: Activate remote control by [3]
• Item [4]: Select baud rate of serial interface by [4]
• Item [5]: Download of operating system to FLASH MEMORY by [5]
Select item.
Item 1: External printer with serial interface

S PRINTER OFF The present status of the printer is displayed in the first line.
1/ON 0/OFF Activate printer by [1] or deactivate by [0].
Item 2: EDP
EDP OFF The present status of the EDP interface is displayed in the first line.
1/ON 0/OFF Activate EDP by [1] or deactivate by [0].
EDP CONFIG. 1 In case of activation:

0/S-E-B 1/CR-LF The present status of the EDP protocol is displayed in the first line.
Select STX / ETX / BCC protocol by [0] or select CR / LF protocol by [1].
Item 3: Remote control

REMOTE Confirm with [ENTER].
ON The keyboard is turned off. The control of the device has to be done by e.g. a
computer.
Normally the remote control mode can be stopped by [BREAK].
With a suitable program the Photometer 5010 can be remote controlled by a PC,
connected by a 1:1 data cable (REF 501-002) to the RS-232 serial interface.
Item 4: Baud rate

The present status of the Baud rate is displayed in the first line.
BAUD RATE 9600 Select 4800 bps by [0] or select 9600 bps by [1].
0/4800 1/9600

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Item 5: Download to FLASH MEMORY

The FLASH MEMORY is a non-volatile memory which may be erased and
DOWNLOAD programmed electrically. This feature allows replacing the operating system of
INPUT +/E/B Photometer 5010.
The process is done with utility DOWNLOAD and a software module running on a
personal computer. Both devices, Photometer 5010 and the personal computer,
have to be connected by a serial interface cable.
For further information see SERVICE MANUAL chapter 6.3 – Download to

FLASH MEMORY.
5.9 Changing Sample Numerator

- Possible only within a course of method with [MODE] [NUM.] -
The sample numbering can start with any number between 0 and 99. The
modification of numbering can be carried out also during a sequence of
measurement. The new numbering gets effective after triggering of a measuring.
SAMPLE NO. Enter next number and confirm with [ENTER].

1
5.10 Multi-standard tools

Linear calibration is used in the case of two calibrators. The assay absorbance at different concentrations forms
a linear plot.
Nonlinear calibration is used for those sample constituents where assay absorbance at different concentrations
forms a nonlinear but reproducible plot. At least three (maximum 20) calibrators are required for nonlinear
calibration.
A multi-standard method - built up on the basic methods - can be created and stored with the method editor
(chapter 7 - METHOD EDITOR). The prototype of the method (basic method) has to be a calculation procedure
based on endpoint with factor (CP 1, 2, 3 and 4), fixed time kinetic with factor (CP 9) or kinetic with factor
(CP 11). The new method has to be saved marked “MULTI STD” in the range of method number 20 to 249.
For the programmed method a nonlinear calibration curve is assigned with the same number. Up to 50
nonlinear calibration curves with maximal 20 sets of points (calibrators) can be stored.
A special mode function opens a submenu with four multi-standard tools (chapter 5.2 - MODE FUNCTIONS).
Start menu by [MODE] [5]:
MENU MULTI STD Item [1]: Measurement and storing of calibrators

1-4/+ Item [2]: Graphic print-out of curve
Item [3]: Edit given data points of a curve
Item [4]: Show all stored multi-standard methods
Select item.

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Item 1: Measurement and storing of calibrators

The measurement of the calibrators can be performed.
Start menu by [MODE] [5] [1].
MEASURE CURVE
NEXT KEY E/B/+ Confirm with [ENTER].
CURVE NO. 20 The number of the pre-selected calibration curve (e.g. 20) is displayed. Confirm
NEXT KEY E/B/# with [ENTER] or change the contents according to another existing calibration
curve.
METHOD NO. The main menu is displayed. The system is prepared in a special mode for
constructing the selected calibration curve.
INPUT 1-250
The system is ready to measure all calibrators one after the other. The procedure
is performed by one of the basic methods using a standard. Valid basic methods
are:
• 5.5.5 - Concentration Measurement with Standard

• 5.5.6 - Concentration Measurement with Standard and Reagent Blank
• 5.5.7 - Concentration Measurement with Standard and Sample Blank
• 5.5.8 - Concentration Measurement with Standard, Sample Blank and
Reagent Blank
• 5.5.10 - Fixed Time with Standard
• 5.5.12 - Kinetic with Standard
M5 Call e.g. method 5 (Concentration Measurement with Standard).

Enter wavelength, given concentration of calibrator, temperature control and
C/S
delay.
Insert blank into the cuvette compartment or sip blank solution.

Press [ZERO].
Insert calibrator into the cuvette compartment or sip calibrator solution.

Press [RESULT].
0.301 0 The first absorbance (e.g. 0.301) of the calibrator is shown.

The cursor marks the second value of the absorbance (e.g. 0).
0 E/+/0
With [+] skip to the next value.
With [0] the selected value is deleted.
In case of double determination: With [RESULT] the next reading of the calibrator
is performed.
In case of triple determination: With [RESULT] the next reading of the calibrator is
performed.
With [ENTER] the value is accepted.
M: 20 A: 0.301 In the first line the number of the calibration curve (e.g. M: 20) and the
P: 1 C: 60 absorbance of the calibrator (e.g. A: 0.301) is displayed.
In the second line the number of the data point (e.g. P: 1) and the concentration
of the calibrator (e.g. C: 60) ) is displayed.
The number of the data point can be edited between 1 and 20.
NEW STANDARD With [ENTER] the value is stored as actual data point. On parallel a print-out with
all data is created (see below: Print-out of calibration procedure).
#
The next calibrator can be measured and stored as data point.
After the last measurement and storing of calibrator the procedure can be finished
by [BREAK], [BREAK].
CURVE STORE The calibration curve is stored with [1] or canceled with [0].
0/NO 1/YES In case of storing the set of data points will be sorted by ascending absorbance.

5010e_44.doc / 02.04.05
Print-out of calibration procedure
PHOTOMETER 5010 # 9876

DATE: 09.12.02
TIME: 17:23:19
METHOD 5: C/S
PROGRAM: 5
STANDARD: 60
WAVELENGTH: 405nm
TEMPERATURE: OFF
DELAY: 0s
UNIT:
CURVE: 20
STANDARD: 60
MEASURE BLANK
ST[A] 1: 0.302
ST[A] 2: 0.304
ST[A] 3: 0.301
ST[A] : 0.302
DATA POINT: 1
STANDARD: 30
ST[A] 1: 0.601
ST[A] 2: 0.602
ST[A] 3: 0.599
ST[A] : 0.600
DATA POINT: 2
STANDARD: 15
ST[A] 1: 0.920
ST[A] 2: 0.922
ST[A] 3: 0.919
ST[A] : 0.920
DATA POINT: 3
STANDARD: 7.5
ST[A] 1: 1.160
ST[A] 2: 1.162
ST[A] 3: 1.157
ST[A] : 1.160
DATA POINT: 4
STANDARD: 3.25
ST[A] 1: 1.501
ST[A] 2: 1.499
ST[A] 3: 1.504
ST[A] : 1.501
DATA POINT: 5

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Item 2: Graphic print-out of curve

A nonlinear curve and the given data points can be printed.
PRINT CURVE
CURVE NO. 20 The number of the pre-selected calibration curve (e.g. 20) is displayed. Confirm
NEXT KEY E/B/# with [ENTER] or change the contents equivalent to another existing calibration
curve.
Plot of nonlinear curve

The given data points are marked with dots.
Table of given data points P1 up to P5
Item 3: Edit given data points of a curve

The procedure allows editing of an existing nonlinear curve.
EDIT CURVE
CURVE NO. 20 Select nonlinear curve by numeric key.

NEXT KEY E/B/#
A 0.302 P 1 Continue with editing described in

C 60 EZB+- chapter 7 - METHOD EDITOR: Editing of standards

5010e_44.doc / 02.04.05
Item 4: Show all stored multi-standard methods

The procedure shows the existing nonlinear curves and their assigned methods.
PARAM.METHODS
M20 05.29.02 5 Displayed is e.g. the existing nonlinear curve 20, created on 05.29.02, based on
NEXT KEY E/B five given data points.
Confirmation with [ENTER] skips to the next curve or finishes the procedure.
Example of print-out for method 20
PHOTOMETER 5010
DATE: 05.29.02
TIME: 17:34:25
METHOD 20: DEMO
PROGRAM: 1
FACTOR: 1.000
WAVELENGTH: 405nm
TEMPERATURE: 25C
DELAY: 1s
UNIT: ng/dl
MULTI STD
MEASURE BLANK
NO. ABS. RESULT

Result with a concentration of e.g. 24.177 ng/dl.
1 0.700 24.177
The absorbance has a value of 0.700 A.
2 1.001 12.107
3 1.303 5.526
Result with a concentration out of range. 4 1.570 <<<>>>
5 0.308 59.340
Result with a concentration out of range. 6 0.292 <<<>>>

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5.11 Patient data management

Up to 2970 results of patients can be managed in the memory of Photometer 5010. The results are stored
together with a two-digit patient number. The range of the patient numbers is starting with 1 and ending with 99.
The memory is available after switching off the instrument and dependent on the lifetime of the battery.
The memory is not a FIFO. Therefore no results can be lost. If the memory is filled with 2970 results, an addition
of further results is not able. The memory has to be emptied first by deleting results assigned to patient
numbers. In practice, the operator is asked for deleting the results after each print-out of patient data.
Up to 2970 results can be assigned to one patient number. On average 30 results can be stored in case of
working with 99 patient numbers.
5.11.1 Working with patients data within a course

The following procedure shows an example of storing results of a patient within a course.
Once the patients mode has to be activated by [MODE] [3] [1] (see next chapter).
86 12.5 mg/dl Within a course the two digit numerator of results is used as patient number.
Before measuring a sample enter patient number by using the MODE-function
MEASURE SAMPLE
[MODE] [NUM] (see chapter 5.9 - Changing Sample Numerator).
SAMPLE NO. Last patient number was e.g. 86. Confirm this number with [ENTER],
86 23 or enter current patient number, e.g. 23, and confirm with [ENTER].
86 12.5 mg/dl Measure next sample by [RESULT].

MEASURE SAMPLE
23 12.9 mg/dl Store result by [1] or skip over without storing by [0].
STORE ? 0/N 1/Y
5.11.2 Patient data management utilities

Start menu by [MODE] [3]:
MENU PAT. 1-4/+ Item [1]: Activate or deactivate patient mode

Item [2]: Print-out of patient related results *
Item [3]: Delete patients data *
Item [4]: Print-out of memory occupancy *
* Patient mode must be activated.
Select item.
Item 1: Activate or deactivate patient mode

PATIENT ON/OFF Confirm with [ENTER].

NEXT KEY E/B/+
P-MODE OFF The present status of the patient mode is displayed in the first line.
1/ON 0/OFF Activate patient mode by [1] or deactivate by [0].
In case of a deactivated patients mode no more items are shown in the menu.
No results can be lost by deactivating patient mode.

5010e_44.doc / 02.04.05
Item 2: Print-out of patient related results

A survey of all stored results is printed for a patient number.
PRINT PATIENT Confirm with [ENTER].

NEXT KEY E/B/+
PATIENT NO. The last selected patient number is shown in the second line (e.g. 6).
Enter desired patient number (e.g. 20).
6 20
The print-out of results is started:
**** PATIENT REPORT ****

PHOTOMETER 5010 #3501
DATE: 12.18.02
LAB.: Labor xxxxx
PATIENT NO.: 20
DATE: 12.11.02
METHOD 55: tBIL.dcp
100 mg/dl
DATE: 12.12.02
METHOD 55: tBIL.dcp
104 mg/dl
DATE: 12.14.02
METHOD 55: tBIL.dcp
102 mg/dl
DATE: 12.15.02
METHOD 73: CAmtb
5.4 mg/dl
DATE: 12.16.02
METHOD 55: tBIL.dcp
101 mg/dl
DATE: 12.17.02
METHOD 73: CAmtb
5.1 mg/dl
DELETE PATIENT With [0] quit without deleting.

0/NO 1/YES With [1] quit with deleting printed data.
At select [1]:
ARE YOU SURE ? With [0] quit without deleting.
0/NO 1/YES With [1] quit with deleting.

5010e_44.doc / 02.04.05
Item 3: Delete patients data

DELETE PATIENT Confirm with [ENTER].

NEXT KEY E/B/+
PATIENT NO. The last selected patient number is shown in the second line (e.g. 6).
Input desired patient number (e.g. 20).
6 20
ARE YOU SURE ? With [0] quit without deleting.

0/NO 1/YES With [1] quit with deleting all results concerning selected patient number.
Item 4: Print-out of memory occupancy

A survey of all stored patient numbers with the number of results is printed.
PRINT P-MEM.INF Confirm with [ENTER].

NEXT KEY E/B/+
The print-out shows maximum three sets of values at a line. A set of values
consists of the patient number and the number of stored results separated by a
slash. For example 5/ 4 means: 4 results of patient number 5 are stored in the
memory.
**** PATIENT MEMORY ****

DATE: 12.18.02
5/ 4 20/ 12 75/ 3
17/ 14 89/ 1 46/ 27

5010e_44.doc / 02.04.05
6 USER GUIDANCE AT DISPLAY
DISPLAY DESCRIPTION
Method selection:
METHOD NO. See chapter 4 - SELECTION OF MEASURING PROGRAMS
INPUT 1-250
Sip rinse solution or next sample by pushing sipping lever [P].

PUMP -> P
Method selection:
M1 The method M1 – C/F is selected.
C/F See chapter 4 - SELECTION OF MEASURING PROGRAMS
Filter selection:
M1 FILTER Select actual wavelength by [ENTER].
F1 340NM +-# Change wavelength by [+], [-] or numeric key [#].
Factor, standard, etc.:

M1 FACTOR Enter the value by numeric key [#].
#
Temperature level selection:

M1 TEMPERATURE Change temperature level by [+], [-] or numeric key [#].
0 OFF +-#
Delay selection:
M1 DELAY Enter the value in seconds by numeric key [#].
s #
Supply and setting to zero of zero solution depending on cuvette system

M1 (see chapter 3.7 - CUVETTES AND CUVETTE ADAPTOR) by [ZERO].
MEASURE BLANK Then setting to zero is displayed by a 0 in the first line and printed out.
Setting to zero can be repeated within a series of measuring.
Supply and measurement of sample solution depending on cuvette

0 Z[A] system (see chapter 3.7 - CUVETTES AND CUVETTE ADAPTOR).
MEASURE SAMPLE
In the first line the result 61 of sample no. 1 is shown.

1 61 R[ ] Supply and measurement of sample solution depending on cuvette
MEASURE SAMPLE system (see chapter 3.7 - CUVETTES AND CUVETTE ADAPTOR).
In the first line the result 61 mg/dl of sample no. 1 is shown.

1 61 mg/dl Supply and measurement of sample solution depending on cuvette
MEASURE SAMPLE system (see chapter 3.7 - CUVETTES AND CUVETTE ADAPTOR).
Supply and measurement of reagent blank solution depending on

0 Z[A] cuvette system (see chapter 3.7 - CUVETTES AND CUVETTE
MEASURE Rb ADAPTOR).
Instead of being measured, the reagent blank can also be entered via
the keyboard or put on zero.
Supply and measurement of blank of reagent blank solution depending

0 Z[A] on cuvette system (see chapter 3.7 - CUVETTES AND CUVETTE
MEASURE Rbb ADAPTOR).
ROBERT RIELE GmbH & Co KG Page 41 6 - USER GUIDANCE AT DISPLAY

5010e_44.doc / 02.04.05
DISPLAY DESCRIPTION
Supply and measurement of sample blank solution depending on cuvette

MEASURE Sb
Enter given concentration value of standard including decimal point.

M5 STANDARD
#
The last determined resulting factor of a standard measuring is saved

OLD STD 415 together with the corresponding method no. After renewed selection of
0/NO 1/YES the same method no. this resulting factor is offered as „Old STD“. In all
other cases the data of the standard get rejected.
Supply and measurement of standard solution depending on cuvette

MEASURE ST
Enter incubation time in seconds.

M9 INCUBATION
s #
Enter reaction time in seconds.

M9 REACTION
s #
With [ENTER] the result is accepted. The next measurement can be

1 0.243 R[ ] started.
NEXT KEY E/R/Z/B With [RESULT] a new measurement replaces the old one without
touching the numerator.
With [ZERO] a zero adjustment is made.
With [BREAK] the method is finished.
Enter number of deltas of a kinetic method.
M11 DELTAS
#
Enter time per delta of a kinetic method.

M11 TIME/DELTA
s #
While performing the course the dialog asks for the use of a reagent
RB OFF blank.
1/ON 0/OFF The default value is OFF.
To continue without reagent blank press [ENTER].
Sample no. 1:
1 0.701 R[ ] Initial value of absorbance: 0.701
DELTA 1 5d1
Sample no. 1:
1 -0.021 /MIN. Delta 3 in absorbance / minute: -0.021
DELTA 3 5d1
ROBERT RIELE GmbH & Co KG Page 42 6 - USER GUIDANCE AT DISPLAY

5010e_44.doc / 02.04.05
7 METHOD EDITOR
Based on the 13 calculation procedures, up to 230 user-defined methods can be created and saved.
Abort of method editor and change back to program selection with [BREAK] at any time. So the program can be
used like a viewer.
Every input has to be confirmed with [ENTER].
Changing of attributes of the fixed defined methods in the range of 1 up to 19 is not possible.
Start menu with [MODE] [EDIT]:
METHOD EDIT Confirm with [ENTER].

NEXT KEY E/B
SOURCE 1 Enter the number of the method (e.g. 1 -> C/F, see chapter 13.1 - BASIC
INPUT 1-250 METHODS) which is to become the prototype (SOURCE) of the new method.
TARGET 20 Enter the number of the new method (TARGET e.g. 20).
INPUT 20-249 Confirm with [ENTER].
A corresponding error message (ERROR 16) is carried out if the space of method
memory is already occupied. When required, the method in question can be
erased (Utility program 8.2.4 - Deleting method) and the space of method
memory can be occupied newly.
In the range of 20 up to 249 an existing method can be changed by using the
same number for the prototype method as for the new method.
COPY ? • Item [1]: The new method will be created as method 20.
0/NO 1/YES Method 20 is identical with method 1. The operation will be
finished.
• Item [0]: The procedure allows constructing the new method with different
attributes. The queue of attributes is dependent on the attributes of
the prototype. Generally default inputs can be confirmed by
[ENTER] or replaced by new inputs.
Select item.
In case of selecting item 0:
PROG. C/F 1 Input of name of new method ( e.g. ABC ). The string is limited to eight
NAME ABC characters.
The number keys are each occupied four times (see TEXT EDITOR LEVEL).
The desired character is selected by repeated push of the corresponding key.
Key [7]: A...B...C...D Key [8]: E...F...G...H Key [9]: I...J...K...L

Key [4]: M...N...O...P Key [5]: Q...R...S...T Key [6]: U...V...W...X
Key [1]: Y...Z...1...2 Key [2]: 3...4...5...6 Key [3]: 7...8...9...0
Key [0]: /...#...%...-
With key [WASH / A ↔ a]: Change between capital and lower letters
(flashing cursor = lower letters)
With key [ • / Chr. →]: Change of the cursor to the next input position
With key [ENTER]: Accepting and ending the input
Correction of input: By change of the cursor beyond the last place
and new input as of the first place
ROBERT RIELE GmbH & Co KG Page 43 7 - METHOD EDITOR

5010e_44.doc / 02.04.05
PROG. Input of unit of new method ( e.g. g/l ). The string is limited to six characters.
UNIT g/l The input is done by the same procedure like creating the name (see above).
PROG.FACTOR Input of factor of new method ( e.g. 123.4 ).

0 123.4 # In case of a method based on standard the first line is replaced by
PROG.STANDARD.
PROG.FILTER Input of wavelength of new method ( e.g. 340 nm ) by [+], [-] or numeric key [#].
F1 340NM +-#
PROG.TEMPERATURE Input of temperature control of new method ( e.g. 3 ).

3 37C +-# • Selection 0 means: Temperature control OFF
• Selection 1 means: Temperature control 25 °C
PROG.VOLUME Input of sipping volume of new method ( e.g. 6 ) in steps of 50 µl in the range of
6 500ul +-# 250 µl up to 2000 µl.
• Selection 1 means: 250 µl
In case of calculation procedure end point (CP 1 up to CP 8, CP 13):
PROG.DELAY Input of delay before every measuring ( e.g. 5 s ).

0s 5s +-# The query of the delay can be suppressed by entering [65535].
PROG.MIN.UNITS Enter lower limit of a measurement result 5-digit + comma + sign.

0 # A zero deactivates this option.
If the measured value falls below the entered one, the error message "ERROR
MIN. UNITS" is shown.
PROG.MAX.UNITS Enter upper limit of a measurement result 5-digit + comma + sign.

If the measured value exceeds the entered one, the error message "ERROR
MAX.UNITS" is shown.
In case of calculation procedure fixed time (CP 9 and CP 10):
PROG.INCUBATION Input of incubation time before the first measuring ( e.g. 30 s ).

0s 30s # The query of the delay can be suppressed by entering [65535].
PROG.REACTION Input of reaction time before the first measuring ( e.g. 60 s ).

0s 60s # The query of the delay can be suppressed by entering [65535].
PROG.MIN.UNITS Enter lower limit of a measurement result 5-digit + comma + sign.

If the measured value falls below the entered one, the error message "ERROR
MIN. UNITS" is shown.
PROG.MAX.UNITS Enter upper limit of a measurement result 5-digit + comma + sign.

MAX.UNITS" is shown.

5010e_44.doc / 02.04.05
In case of calculation procedure kinetic (CP 11 and CP 12):
PROG.DELAY Input of delay before every measuring ( e.g. 5 s ).

0s 5s +-# The query of the delay can be suppressed by entering [65535].
PROG.DELTAS Input of deltas in the range of 3 up to 28 (e.g. 3).

0 3 #
PROG.TIME/DELTA Input of time per delta in the range of 4 up to 255 (e.g. 20).
0s 20 s #
PROG.MIN.R^2 Input of low limit of the correlation coefficient R^2 (e.g. 0.998) for linearity control.
0 0.998 # A zero deactivates this option.
If the measured value falls below the entered one, the error message
"ERROR R^2" is shown.
PROG.MAX.UNITS Enter upper limit of the measurement result in Units with right plus or minus sign
0 0 # (+ climbing / - falling Kinetic).
A zero deactivates this option.
MAX.UNITS" is shown, at Kinetics additionally "ERROR+/-".
All other cases:
PROG.MULTI STD • Item [0]: The new method will be created as method with linear
0/NO 1/YES characteristic.
• Item [1]: The new method will be created as method with nonlinear
characteristic based on polygonal interpolation. From a set of given
data points, a new set of points is computed representing the
breakpoints of a polygon approximating the function searched for.
Maximum 20 data points (standards P1 up to P20) can be used as
given data points.
Select item.
In case of selecting item 0 (linear):
STORE ? Select [0] to leave method editor without changing parameters.

0/NO 1/YES Select [1] to store all parameters of method and to finish the operation:
In case of selecting item 1 (nonlinear / polygonal interpolation):
The given data points (standards/calibrators P1 up to P20) have to be entered.

The prototype of the method (basic method) has to be a calculation procedure based on endpoint with factor
(CP 1, 2, 3 and 4), fixed time kinetic with factor (CP 9) or kinetic with factor (CP 11).
P1 is the standard with the lowest absorbance. P20 is the standard with the highest absorbance.
The following table shows an example of five given data points:
Data point Absorbance A Concentration C

P1 0.300 60
P2 0.600 30
P3 0.920 15
P4 1.160 7.5
P5 1.500 3.25

5010e_44.doc / 02.04.05
Editing of standards:
General operation:
With [+] or [-] skip to the next data point.
With [Z] the selected data point is deleted. The values of A and C show: -00000
With [ENTER] the input of the current value is accepted.
With [BREAK] the procedure of input is finished.
A -00000 P 1 Enter the values of the first standard P1:

C -00000 EZB+- In the first line the absorbance A (e.g. 0.3) has to be entered. Confirm input with
[ENTER].
Select second line of display with [ENTER].
In the second line the concentration or activity C (e.g. 60) has to be entered.
Confirm input with [ENTER].
Select next standard P2 with [+] or [-].
A -00000 P 2 Enter the values of the second standard P2 (see above).

C -00000 EZB+-
Select next standard P3 with [+] or [-].
A -00000 P 3 Enter the values of the third standard P3 (see above).

C -00000 EZB+-
Continue with inputs including the last standard. Maximum 20 standards can be
entered.
Finish the input with [BREAK].
STORE ? Select [0] to leave method editor without changing parameters.

0/NO 1/YES Select [1] to store all parameters of method and to finish the operation:
In case of storing the set of data points will be sorted by ascending absorbance.
A printing of data table and curve can be done (see chapter 5.10 - Multi-standard
tools: Graphic print-out of curve).

5010e_44.doc / 02.04.05
8 UTILITY PROGRAMS
8.1 SELECTION OF UTILITY PROGRAMS
METHOD NO. Method selection: change to utility program selection with [MODE].
INPUT 1-250
Confirm with [MODE].
INPUT 1-n/+ Utility program selection: direct selection of required utility program, i.e. input of
UTILITIES concerning identity no. (see table) and confirming with [ENTER], or by scrolling
through all utility programs with [+] ... [+].
Abort of utility program selection and return to program selection with [BREAK].
A utility program can be broken off with [BREAK] at any time with return to the
selection of utility programs.
After working off a utility program automatic return to the selection of utility
programs.
Identity-No. Utility Program Description

in chapter
1 Dark level adjustment 8.2.1

2 Adjustment of bubble detector 8.2.2
3 Error list 8.2.3
4 Deleting method 8.2.4
5 Language selection 8.2.5
6 Beep ON / OFF 8.2.6
7 Temperature control ON / OFF 8.2.7
8 Temperature calibration 8.2.8
9 AD-Transducer 8.2.9
10 ADC dark current values 8.2.10
11 Display test 8.2.12
12 Printing device configuration 8.2.12
13 Real time clock ON / OFF 8.2.13
14 Filter wheel test 8.2.14
15 Temperature correction single cuvette adaptor 8.2.15
16 Temperature correction flow-through adaptor 8.2.16
17 Printing user defined methods 8.2.17
18 Laboratory name 8.2.18
19 User name 8.2.19
ROBERT RIELE GmbH & Co KG Page 47 8 - UTILITY PROGRAMS

5010e_44.doc / 02.04.05
8.2 DESCRIPTION OF UTILITY PROGRAMS
8.2.1 Dark level adjustment

The dark level adjustment should be carried out not before the warm-up time of 15 minutes has passed, better
after one hour operation.
To avoid strange light the lid must absolutely be closed during the dark level adjustment.
Start menu by [MODE] [MODE] [1]:
DARK ADJ. U1 Confirm with [ENTER].

UTILITIES
DARK ADJ. U1 Automatically all eight sensitivity levels (V0...V7) are calibrated in succession at
V0 82 simultaneous display of the dark value.
8.2.2 Adjustment of bubble detector

With this test the function of bubble recognition can be adjusted and checked. The adjustment can only be done
with the flow-through cuvette adaptor.
MEDIA TEST U2 Confirm with [ENTER].

UTILITIES
MEDIA TEST U2 Sip distilled water by [WASH] or sipping lever [P]. Be sure that the aspirate tube is
0 AIR filled with water.
Enter new sensitivity level step by step by numeric key [0] up to [7]. When the
display changes from “AIR” to “WATER”, increase the sensitivity level by one
further step.
MEDIA TEST U2 The display shows the current sensitivity level of the bubble detector.
3 WATER
MEDIA TEST U2 Empty the aspirate tube by [WASH]. The display changes from “WATER” to
3 AIR “AIR”.
MEDIA TEST U2 Sip distilled water by [WASH] or sipping lever [P]. The display changes from “AIR”
3 WATER to “WATER”.
Confirm adjustment with [ENTER].
8.2.3 Error list

With this utility the last 10 errors are shown or printed.
ERROR LIST U3 Confirm with [ENTER] for showing the errors subsequently
UTILITIES or confirm with [LF] for printing the errors.
In case of [ENTER]:
ERROR LIST U3
The error no. 10 is dated 07.23.01. The error code is 8.
10*07.23.01* 8 Continue with [ENTER] to show next error.
The error no. 1 is the error occurred at last.

5010e_44.doc / 02.04.05
8.2.4 Deleting method

With this utility program one of the methods edited by the user can be deleted.
METHOD CLEAR U4 Confirm with [ENTER].

UTILITIES
Enter number of the method to be erased in the range of 20 up to 249.

METHOD NO. After confirmation with [ENTER] the method in question is immediately erased
INPUT 20-249 without further query.
query
8.2.5 Language selection

With this utility program the language of the display and print-out can be selected.
LANGUAGE U5 Confirm with [ENTER].

UTILITIES
Select English language with [1] or German language with [2].

1/GB 2/D The selected setting is active immediately.
UTILITIES
8.2.6 Beep ON / OFF

The beeper can be switched on or off. The deeper signal tone for error messages remains active in every case.
BEEP ON/OFF U6 Confirm with [ENTER].

UTILITIES
The current status is displayed in the upper line.

BEEP ON Switch the signal tone off with [0].
1/ON 0/OFF Switch the signal tone on with [1].
8.2.7 Temperature control ON / OFF

The electrical heating/cooling on 25 °C, 30 °C or 37 °C can manually be switched on or off.
TEMP. ON/OFF U7 Confirm with [ENTER].

UTILITIES
The actual status to the heating/cooling is displayed in the upper line.

HEATING OFF Switch heating/cooling on with [1] and off with [0].
1/ON 0/OFF
In case of activated heating/cooling:

HEATING ON Select temperature level by [1] = 25 °C, [2] = 30 °C or [3] = 37 °C.
1/25 2/30 3/37 The setting is taken temporarily. It remains unchanged either up to switching on
the device next time or up to the selection of a method with other temperature
adjustment.

5010e_44.doc / 02.04.05
8.2.8 Temperature calibration
The temperature control was adjusted at the factory. In case of e.g. lost of data the heating/cooling
control can be calibrated if the heating/cooling is switched on (for at least 30 minutes):
Measure the current temperature with an independent measuring system (e.g. thermistor, REF 090-063) inside
the cuvette and enter this value. According to the difference to 25.0 °C, 30.0 °C or 37.0 °C the system corrects
its internal setting. The calibrating of the temperature is interrupted when the heating/cooling is off or the
temperature unstable.
TEMP. CALIBR. U8 Confirm with [ENTER].

UTILITIES
Enter the password “5010” (as query). Confirm with [ENTER].

TEMP. CALIBR. U8
PASSWORD
Enter the temperature currently measured always four-digit in °C (e.g. 37.20).

INPUT DEGREE C
37C 37.20
8.2.9 AD-Transducer readings

Display of the current readings of the analogue-digital-transducer (proportional to the brightness on the photo
detector) in dependency on the selected sensitivity level.
ADC COUNTS U9 Confirm with [ENTER].

UTILITIES
Boost operation is deactivated. Activating with [1], deactivating again with [0].
BOOST OFF Accept the displayed status with [ENTER].
1/ON 0/OFF
ADC COUNTS U9 On the second line the current sensitivity level (e.g. 0) in the range 0…7 is shown
0 4711 F1 on the left and the current AD-transducer value (e.g. 4711) in the middle. On the
right the actual filter position (e.g. F1) is shown.
The current sensitivity level can be changed by numeric keys [0] up to [7].
The filter position can be changed by [MODE] and a numeric key [1] up to [9].
Filter positions 1 up to 8 are used for interference filters.
Filter position 9 is used for dark position of filter wheel.
Sip distilled water by [WASH] or sipping lever [P] in case of using flow-through
adaptor before measuring.
8.2.10 ADC dark current values

Display of the offset values for the AD-transducer, determined and saved at the dark level adjustment, in
dependency on the selected sensitivity level.
ADC DARK U10 Confirm with [ENTER].

UTILITIES
On the second line the sensitivity level in the range 0…7 is shown on the left.
ADC DARK U10 The corresponding saved dark values in combination with the single cuvette
0 E 81 D 88 adaptor (e.g. E 81) and the flow-through adaptor (e.g. D 88) are shown.
Selection of another sensitivity level by pushing the corresponding number key.

5010e_44.doc / 02.04.05
8.2.11 Display test

The physical working order of the display can be checked.
DISPLAY TEST U11 Confirm with [ENTER].

UTILITIES
A black rectangle appears at each digit of the display.

AY TEST U11 At the end of the operation each pixel is activated.
UTILITIES After working off the utility program confirm with [ENTER].
8.2.12 Printing device configuration

Print-out of the program version and the complete status of the stored variables.
PRINT DEF. U12 Confirm with [ENTER].

UTILITIES
DATE: 09.11.03
TIME: 19:20:38
V4.0a 03.07.03 D/2.0/1.0
PCB LAYOUT a
ADC DARK E
0: 16964 1: 16964
2: 16963 3: 16962
4: 16959 5: 16951
6: 16936 7: 16914
ADC DARK D
0: 16967 1: 16967
2: 16968 3: 16968
4: 16967 5: 16966
6: 16965 7: 16965
TEMPERATURE E
25C 10500 3000
30C 11750 3000
37C 13500 3000
TEMPERATURE D
25C 10500 3000
30C 11750 3000
37C 13500 3000
FILTER
1: 340 2: 405
3: 546 4: 578
5: 492 6: 623
7: 999 8: 999
PUMP
MS AIR 240
MS 50ul 70
MEDIA TEST 0
VOLTAGES
12V: 12.00V
15V: 14.88V
VCC: 5.00V
+-12V: 12-0.02V
BATTERY: OK
ADC CORRECT. 75
BOOST NM 390
EDP ON STX-ETX-BCC
LANGUAGE GB
BEEP ON
PROGR. METHODS 0

5010e_44.doc / 02.04.05
8.2.13 Real time clock ON / OFF

The real time clock can be switched OFF and ON. Date and time can be set.
CLOCK ON/OFF U13 Confirm with [ENTER].

UTILITIES
The real time clock is activated. Deactivating with [0], activating again with [1].
CLOCK ON Accept the displayed status with [ENTER].
1/ON 0/OFF
After deactivating and activating real time clock:

DATE mm.dd.yy Enter month, day, year, hour, minute and second. Confirm each one with
TIME hh:mm:ss [ENTER].
Enter month, day, year, hour, minute and second. Confirm each one with
DATE 07 25 01 [ENTER].
TIME 20 11 00
8.2.14 Filter wheel test

The filter wheel can be tested.
F-WHEEL TEST U14 Confirm with [ENTER].

UTILITIES
Select filter position in the range of 1 up to 9.

F-WHEEL TEST U14 Accept the displayed status with [ENTER].
EDIT 1-9
On the second line the current filter position (e.g. F2) and the stored wavelength
F-WHEEL TEST U14 (e.g. 405 nm) is shown.
F2 405NM 47MS Finish the test with [BREAK].
8.2.15 Temperature correction single cuvette adaptor

Display of the current setting. The correction of the temperature regulation is possible by change of the
temperature setting. A change of about 25 counts means a temperature change of 0.1 °C.
E-CUV. CORR. U15 Confirm with [ENTER].

UTILITIES
E-CUV. CORR. U15 Select temperature level by [1] = 25 °C, [2] = 30 °C or [3] = 37 °C.
1/25 2/30 3/37
Enter new temperature value and confirm with [ENTER] or finish operation with
E-CUV. 37C
[BREAK]. The display shows for example the value 3000 (default) for the
3000
temperature level 37 °C.
Finish the test with [BREAK].

5010e_44.doc / 02.04.05
8.2.16 Temperature correction flow-through adaptor

Display of the current setting. The correction of the temperature regulation is possible by change of the
temperature setting. A change of about 25 counts means a temperature change of 0.1 °C.
D-CUV. CORR. U16 Confirm with [ENTER].

UTILITIES
D-CUV. CORR. U16 Select temperature level by [1] = 25 °C, [2] = 30 °C or [3] = 37 °C.
1/25 2/30 3/37
Enter new temperature value and confirm with [ENTER] or finish operation with
D-CUV. 37C
[BREAK]. The display shows for example the value 3000 (default) for the
3000
temperature level 37 °C.
Finish the test with [BREAK].
8.2.17 Printing user defined methods

Print-out of all user defined methods. Each method is shown in two lines. The first line notes the method number
and the name of the method. The second line notes factor (F) or standard (S) and the value, the
wavelength (nm) and the dimension.
PRINT METH. U17 Confirm with [ENTER].

UTILITIES
METHOD 20: Test 1

F 1234 340nm mg/dl
METHOD 25: Test 2

S 5.555 578nm mmol/l
METHOD 30: Test 3

F 1050 405nm U/l
METHOD 250: C/F

F 1.000 0 nm

5010e_44.doc / 02.04.05
8.2.18 Laboratory name

The name of the laboratory can be stored permanently.
In case of a stored name an additional line within the header is sent to the printer or the EDP.
LAB. NAME U18 Confirm with [ENTER].

UTILITIES
LAB. NAME U18 Edit the name of the laboratory in the second line.
xxxxx For a description of input of names see chapter 7 – METHOD EDITOR.
8.2.19 User name

The names of maximum five users can be stored permanently.
In case of a selected user in the range from 1 to 5 an additional line within the header is sent to the printer or the
EDP. After invoking a method the course asks for the current user:
USER NO. +- 1 The last user is the default name (e.g. Tom).
Tom Select current user from 0 up to 5 by [+] or [-].
In case of selecting user 0 no additional line is in the print-out.
Installing of new user name
USER NAME U19 Confirm with [ENTER].

UTILITIES
USER NAME +- 1 The display shows the name of user number 1.

Select current user from 1 up to 5 by [+] or [-].
USER NAME +- 2 Edit the name of the user number e.g. 2 in the second line.
Maria For a description of input of names see chapter 7 – METHOD EDITOR.

5010e_44.doc / 02.04.05
9 MAINTENANCE
This chapter provides necessary information concerning general maintenance by the user.
If any faults should occur which cannot be remedied, then service should be contacted. Repairs at the
device may be carried out only by authorized specialist staff. Through improper repairs the warranty
extinguishes, and the user can be heavily jeopardized.
9.1 CLEANING INSTRUCTION
Liquid waste is potentially biologically hazardous. Always wear gloves if handling those materials. Do not
touch parts of the device other than those specified. Consult the laboratory protocol for handling biohazardous
materials.
Take care that no liquid enters the device! There is no protection against penetrating of liquids
(Code IP X0). Check from time to time that the tubing and the connections are leakproof.
The flow-through system of the Photometer 5010 has to be washed with dist. water regularly before and after
measuring 2 – 3 times. Depending on sample material and reagent, and always at the end of a working day, the
Photometer 5010 has to be cleaned additionally with a phosphate-free detergents, e.g. with approx. 5ml of
1 % Hitergent solution (REF 5010-024), and afterwards rinsed with 5ml distilled water.
Persistent residues are to be removed with a combined alkaline-acid treatment. The following procedure is
advised step by step:
1. 5 x wash with NaOH 1 N
2. 5 x rinse with dist. water
3. 5 x wash with HCl 0.5 N
If the system is contaminated severely, it may alternatively be cleaned with hypochlorite. The following
procedure is advised step by step:
2. 2 – 3 x wash with hypochlorite (1:20 diluted solution) or ISE Cleaning Solution,
undiluted, let it possibly affect up to 20 minutes
3. 5-10 x rinse with dist. water
For device cleaning and surface decontamination purposes use commercial decontaminating solution which are
usually available in clinical chemistry laboratories like Mikrozid® AF Liquid, Bacillol® plus, 3 % Kohrsolin® or
similar solutions. Switch off the device and disconnect it from the mains voltage. Then clean the device with soft
cloth and decontaminating solution.
Empty the drain tank at the end of daily measurement, or whenever filled.
9.2 CALIBRATING MEASURING SYSTEM

At doubtful measurement results a dark level adjustment must be carried out corresponding to chapter 8.2.1.
It is recommended to calibrate the device every four weeks to ensure that it operates optimally.
9.3 ADJUSTMENT OF BUBBLE DETECTOR

See chapter 8.2.2 – Adjustment of bubble detector.
9.4 CALIBRATION OF PERISTALTIC PUMP

See chapter 5.7 – Pump utilities
ROBERT RIELE GmbH & Co KG Page 55 9 - MAINTENANCE

5010e_44.doc / 02.04.05
9.5 CHANGING RIBBON

Open printer cover. Lift used ribbon by pressing “PUSH” on the left. Take ribbon cassette out of the printer.
Take new ribbon cassette out of the wrapping. Turn small wheel on the left in the direction marked by an arrow
till the inked ribbon is tightened.
Put in the ribbon cassette. Pay attention that the paper is to be taken through inked ribbon and cassette. Take
printer paper through the crack of the printer cover and close the printer cover.
9.6 CHANGING PAPER ROLL

See chapter 2.4 – LOADING PRINTER PAPER.
9.7 CHANGING ASPIRATE TUBE

For changing the aspirate tube pay attention to the sequence of the assembly parts as shown below. Then put
aspirate tube without bending from the cuvette area side through the metal inlet tube. Turn screw connection
with fingers into the cuvette.
inlet tube with base
screw connection, milled O-ring washer

5010e_44.doc / 02.04.05
9.8 REPLACEMENT OF LINE FUSES

The Photometer 5010 operates at any line voltage between 90 VAC and 264 VAC at 50/60 Hz without adjustment.
It has two line fuses in series with the power supply. Those fuses are located on the rear panel. To replace
those fuses, unplug the mains cable and remove the fuse holder with the fuses as shown below. The instrument
is delivered with two spare fuses.
Neither use makeshift fuses nor short-circuit the fuse holder!
Specifications of mains fuse:

• dimensions [mm] : 5 * 20
• standard: IEC 60127-2/V
• time-current characteristic: time lag (T)
• voltage rating: 250 V
• rated current: 1.6 A
• marking: T 1.6 A H
mains switch
fuse holder
mains cable connector
fuse holder with fuses

5010e_44.doc / 02.04.05
10 ERROR MESSAGE / CORRECTION
10.1 GENERAL NOTE
Faulty input (e.g. wrong method number or wrong factor), recognized by the user, can be corrected by filling up
the respective entry field with any signs. After replenishing beyond the last position the faulty input is deleted
and the entry field is free again for the renewed correct input.
Error messages by the device are carried out either exclusively via a signal tone (chapter 10.2 – ACOUSTIC
ERROR MESSAGES) or as combination signal tone and display (see below).
1 0,005 R[ ] In the display errors are shown as plaintext (chapter 10.3 – PLAINTEXT ERROR
ERROR MIN.UNITS MESSAGES)
METHOD NO. 300 … or coded with an error number (chapter 10.4 – CODED ERROR MESSAGES).
ERROR 3
Each error message has always to be confirmed with [ENTER].
10.2 ACOUSTIC ERROR MESSAGES

When pressing a key which is not permitted or not meaningful a deeper signal tone still sounds as error
message after the higher signal tone (which is to confirm the keystroke, can be switched off according to
chapter 8.2.6 – Beep ON / OFF). In the display no corresponding error message appears parallel to this. The
operation of the device can directly be continued by the correct keyboard entry.
10.3 PLAINTEXT ERROR MESSAGES
ERROR MIN.UNITS The programmed low limit was under-run by the measurement.
ERROR MAX.UNITS The programmed upper limit was exceeded by the measurement.
ERROR R^2 The square of the correlation coefficient r lies at the kinetic measuring below the
programmed low limit.
ERROR +/- At the kinetic measuring the course of the kinetic is wrong (increasing / falling).
NO METHOD Dialed method is not programmed. Select other method according to method list.
HEATING OFF Heating / cooling is off during temperature calibration.
TEMP. UNSTABLE Temperature is unstable during temperature calibration.
10.4 CODED ERROR MESSAGES
No. (possible) Causes Remedy
1 method is write protected, method can not be cleared by special software

2 check sum of a freely programmed method is wrong program new method
3 forbidden input, wrong number format repeat input in permitted area
4 method not available method editor:
check method no.
ROBERT RIELE GmbH & Co KG Page 58 10 - ERROR MESSAGE / CORRECTION

5010e_44.doc / 02.04.05
5 dark value is absolutely too high (> 16 bit) or higher as the repeat dark level adjustment;
measurement, check lamp / filter; check blank
ADC overflow
6 all multiplexer positions are too bright/too dark at setting to repeat dark level adjustment;
zero check filter / lamp; check blank
7 mathematical overflow, at measurement calculation check filter; check standard;
check measuring solution
8 check sum error in the data record of the dark offset repeat dark level adjustment
9 check sum error in the data record of the device basic setting automatic error remedy
(status, ADC correction)
10 division by a too small value (< 0.001 A) check filter; check standard; check
measuring solution
11 invalid calibration curve Select valid number
12 setting to zero not possible (zero value is < 32768 cycle) check lamp; check filter;
check zero solution
13 setting to zero not possible (zero value is > 983039 cycle) check lamp; check filter;
check zero solution
14 invalid standard measure valid standard solution
15 no parameter memory vacant (too little memory for nonlinear delete a no longer actual nonlinear
methods) method
16 method no. is occupied select other method no.; delete a no
longer actual nonlinear method
17 check sum error in the parameter memory (nonlinear method) program method newly
18 at calculation overflows in nonlinear method check factor; check parameter
19 clock malfunction
20 overflow at Kinetic check measuring solution
29 wrong input of deltas or time per delta restart method
30 battery empty contact service partner
31 communication: wrong data format contact service partner
32 communication: sent data not plausible / not interpretable contact service partner
33 communication: mentioned module does not answer in a check interconnecting cable; check
certain time mentioned module
34 communication: overflow send/receive buffer reduce amount of data at the
communication partner
35 Remote control: wrong method number external software problem
36 Remote control: unknown command external software problem

5010e_44.doc / 02.04.05
37 Remote control: wrong data format external software problem

38 timeout at reception from module 1 switch off/on device
39 check sum error at reception from module 1 switch off/on device
40 timeout at reception from module 2 switch off/on device
41 check sum error at reception from module 2 switch off/on device
42 NAK at reception from module 2 switch off/on device
43 NAK at reception from module 1 switch off/on device
44 error at pump calibration clamp pump tube;
control volume of aspiration;
the pump tube is not clamped; control connectors of tubes;
no liquid was sipped; the plug of the bubble detector has a
to much liquid was sipped when calibrating; bad contact;
the power of the pump is to small; the cable of the bubble detector has a
the motor does not rotate or stops; bad contact;
connectors of tubes leak; adjust bubble detector (OM chapter
bubble detector works incorrect; 8.2.2);
the aspirate tube is blocked; replace aspirate tube (OM chapter
the aspirate tube is discolored 9.7)
45 error at sipping solution clamp pump tube;
adjust bubble detector (OM chapter
the pump tube is not clamped; 8.2.2);
no liquid was sipped; control volume of aspiration;
the power of the pump is to small; control connectors of tubes;
the motor does not rotate or stops; do a calibration of peristaltic pump
connectors of tubes leak; (OM chapter 5.7);
bubble detector works incorrect; the plug of the bubble detector has a
the aspirate tube is blocked; bad contact;
the aspirate tube is discolored the cable of the bubble detector has a
bad contact;
replace aspirate tube (OM chapter
9.7)
46 filter position outside tolerance
47 + 12 VDC out of range contact service partner
50 +/- 12 VDC out of range contact service partner
51 - not occupied -
52 timeout printer internal the internal printer is temporarily
disconnected
53 - not occupied -
54 nonlinear measurement: value out of range check the absorbance of the sample
55 number of given data points < 2 add data points
56 checksum of operating system damaged contact service partner
57 memory of patients data management is completely filled delete patient data
(OM chapter 5.11.2)
58 checksum of patients data damaged delete patient data
(OM chapter 5.11.2)

5010e_44.doc / 02.04.05
11 TECHNICAL DATA
11.1 ENVIRONMENTAL CONDITIONS

Climatic conditions for storage and transport of the packed device:
• Temperature: -25 °C to +70 °C
• Relative humidity: 20 % to 85 %
The Photometer 5010 must be used in an environment that meets the following conditions:
• Temperature: +15 °C to +35 °C
• Relative humidity: 20 % to 85 %
• Not exposed to direct sunlight or other source of direct light (e.g. a spot light)
• Well-ventilated area
• Free from excessive dust
• Free from combustible gases
• Free from vibrations
• Free from electromagnetic wave interference
• Well-distanced from a machine generating a high frequency high voltage (e.g. a centrifuge)
11.2 MINIMAL OPERATION QUALITY

Analogous amplifiers of high sensitivity process strange sizes similarly to measuring signals, so that effective
quantities are produced apparently and worked off as agreed or that amplifiers are over-modulated. When the
strange size is taken away, the device works again as agreed, a short-time change of the operating response is
without influence on the complete function of the device.
11.3 TYPE PLATE

For installation pay attention to the specifications on the type plate.
ROBERT RIELE GmbH & Co KG Page 61 11 - TECHNICAL DATA

5010e_44.doc / 02.04.05
11.4 SHORT SPECIFICATION

Type Semi-automatic, single-beam filter photometer
Light Source Halogen lamp – 12 V, 20 W
Wavelength 340 nm – 800 nm
Wavelength Selection Automatic via 8-position filter wheel :
6 standard interference filters: 340 nm, 405 nm, 492 nm, 546 nm, 578 nm
and 623 nm; 2 positions for optional filter of choice
Photometric Range 0 – 2.5 A
Cuvette System Micro flow cell:32 µl, 10 mm light path interchangeable with normal standard
cuvettes (macro or semi-micro, disposable or special optical glass)
Temperature Control Internal Peltier element, temperature variable, pre-adjusted to 25 °C,
30 °C and 37 °C
Equilibration time for aspirated reaction mixture to reach 37 °C from
ambient temperature: 15 sec
Aspiration System Built-in peristaltic pump driven by stepper motor, programmable
aspiration volume controlled by infrared light barrier
Sipping Volume Minimum 250 µl
Operator Interface Membrane keyboard with 20 keys for direct functions and numeric entries
Data Presentation LCD display: lighted, 2 lines of 16 characters each, easy to read and offering
simple user guidance
Integrated Printer Dot-matrix printer, 24 characters per line
Memory General operating software contained in FLASH MEMORY can be updated
by PC (data file downloaded from Internet, floppy disk or CD ROM)
Languages: English and German
Capacity for 250 pre-programmed test variants
Up to 230 tests can be stored in RAM. Import of data by keyboard or PC
Up to 50 nonlinear calibration curves with max 20 sets of points can be stored
Signal Port RS 232 serial port for connection to an external printer and/or computer
(bidirectional interface)
Measurement Absorbance
Procedures Endpoints with standard, factor or multiple standards, with or without
reagent blank
and/or sample blank
Kinetics with standard, factor or multiple standards, with or without blank
Fixed time with standard, factor or multiple standards, with or without
reagent blank
Curve fitting for nonlinear standard curves
Measuring Time Kinetic: variable from 3 – 28 deltas,
time per delta 4 – 255 sec in 1 sec steps
Fixed time: variable from 0 – 65535 sec in 1 sec steps
Delay Time Programmable from 0 – 65535 sec in 1 sec steps
Dimensions Length 33 cm x width 34 cm x height 18 cm
Weight 6 kg

5010e_44.doc / 02.04.05
11.5 CERTIFICATES
EC Declaration of Conformity
Manufacturer: ROBERT RIELE GmbH & Co KG
Address: ROBERT RIELE GmbH & Co KG

D-13467 Berlin
Germany
ROBERT RIELE GmbH & Co KG declares under sole responsibility that the product:
Product name: Photometer 5010
to which this declaration relates is in conformity with the following standards or other normative documents:
EN 61326-1
EN 61010-1
EN 61010-2-101
following the provisions of Directive 89 / 336 / EWG (EMC), and 73 / 23 / EWG (LVD) as well as
98 /79 / EG (IVD).
This declaration describes an absorption photometer for laboratory use.
Berlin, October 2004
W. Riele

5010e_44.doc / 02.04.05
Technical Specifications
Description according to DIN 58960 part 4
A Identification
A.1 Type of photometer: Photometer 5010

A.2 Model: 5010
A.3 Instruction for use: Photometer 5010, user manual
A.4 Manufacturer ROBERT RIELE GmbH & Co KG

D-13467 Berlin
Germany
DECLARATION OF CONFORMITY:
The above mentioned absorption photometer is in conformity with the following metrological description.
Berlin, October 2004
W. Riele
B Metrological description
B.1 Measuring System

B.1.1 Optical configuration: cf. Figure
B.1.2 Source[s] of radiation: halogen lamp
B.1.3 Spectroscopic apparatus: filter wheel
B.1.4 Radiation detector[s]: photodiode
B.1.5 Cuvette[s]/cell[s]: 10 mm glass or plastic cuvette (square
shaped) or 10 mm flow-through cuvette
B.1.6 Temperature regulation of the cuvette: 25 °C, 30 °C or 37 °C
B.1.7 Displayed units: absorbance, mass concentration,
enzyme activity
B.1.8 Display device: digital display,
absorbance: 0.000 to 2.500
mass concentration: 0.000 to 9999
enzyme activity: 0.000 to 9999
B.2 Method of measurement

B.2.1 Generation of spectral absorbance A(λ) monochromatic measurement
B.2.2 Zero compensation of spectral absorbance manual
B.2.3 Control of the measured spectral absorbance: with an absorption reference
solution (see manual)
B.2.4 Determination[s] of concentration: Lambert-Beer-Equation

5010e_44.doc / 02.04.05
B.3 Specified measuring range

Outside the specified measuring range and under rated operating conditions other than those
stated in section B.4, the maximum permissible errors given in section B.5 can be exceeded.
B.3.1 Spectral absorbance A(λ): 0 A to 2.5 A

B.3.2 Wavelength λ useable for measurements: 340 nm to 800 nm
B.4 Specified Operation conditions

B.4.1 Spectral transmittance of the cuvette: > 75 %
B.4.2 Warm-up time: 15 min
B.4.3 Operating voltage [mains]: between 90 VAC and 264 VAC at 50/60 Hz
B.4.4 Ambient temperature: 15 °C to 35 °C
B.4.5 Sound pressure level SPL < 50 dB
B.5 Maximum permissible errors and other limiting values

B.5.1 Photometric uncertainty of the spectral absorbance: max ± 3 %
B.5.2 Photometric short-time variation coefficient: ≤1%
B.5.3 Uncertainty of wavelengths: max ± 2 nm
B.5.4 Spectral half-width of spectral radiation flux at
detector: ≤ 10 nm
B.5.5 Percentage of wavelength integrated false
radiation ( measured at 340 nm as transmittance
of a cut-off filter NaNO3): ≤ 0.1 %
B.5.6 Uncertainty of temperature regulation: ≤ 0.2 °C
B.5.7 Specimen related carry-over ratio:
measure according to Broughton with
p-Nitro phenol ≤3%
OPTIC CONSTRUCTION
The path of rays is directed from right to left. Put in single cuvette accordingly.
Diaphragm 1 Diaphragm 2 Diaphragm 3 Diaphragm 4
Lens 1 Lens 2 Lens 3 Lens 4

Halogen lamp
Photodiode Cuvette Cuvette-Adaptor Filter Glass for heat absorption

5010e_44.doc / 02.04.05
12 ACCESSORIES AND SPARE PARTS
Kindly contact directly the responsible dealer.
REF Description
5010-014 Aspirate tubing, 2 pcs
1706853001 Axis printer paper
1706870001 Bubble detector with plug
0573655001 Cuvettes of optical glass, 4 pcs
501-002 Data cable serial interface
805-410 Disposable cuvettes, 1000 pcs
1704818001 Dust cover
1704796001 Flow through cuvette adaptor
5010-018 Fuses for line power, 10 pcs
0554871001 Halogen lamp 12V/20W
5010-024 Hitergent, 1000 ml
0369624001 Interference filter 436nm
5010-670 Interference filter 670nm
(other wavelengths on request)
1707175001 Joint inlet tube cuvette
0552402001 Mains cable
5010-005 Operator’s manual
5010-015 Outlet tube cuvette
1268457001 Printer paper, 5 rolls
1010735001 Printer ribbon, 1 pc
0745235001 Printer ribbon, 5 pcs
5010-013 Pump tube with joints
090-064 Secondary calibration standards, fourpart, certified
1704800001 Standard cuvette adaptor
090-063 Thermistor
1707574001 Top cover small for printer
1704834001 Waste tubing, 2 pcs
ROBERT RIELE GmbH & Co KG Page 66 12 - ACCESSORIES AND SPARE PARTS

5010e_44.doc / 02.04.05
13 METHOD LIST
1 - 13..........13 basic methods (chapter 13.1 - BASIC METHODS)

14 - 19........free (reserved for further 6 automatic calculation methods)
20 - 249......up to 230 user specific methods (chapter 13.2 - LIST OF USER SPECIFIC METHODS as copy
master / to be filled out by the user)
250 .............method Absorbance with open parameters
13.1 BASIC METHODS
ROBERT RIELE GmbH & Co KG Page 67 13 - METHOD LIST

5010e_44.doc / 02.04.05
13.2 LIST OF USER SPECIFIC METHODS
ROBERT RIELE GmbH & Co KG Page 68 13 - METHOD LIST

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Operating Instructions

ROBERT RIELE GmbH & Co KG

Software Version 4.4

This product fulfills the requirements of Directive 98/79/EC on

In vitro diagnostic medical device

Caution (refer to accompanying documents)!

Please consult instructions for use

Description of technical specifications according to DIN 58 960, part 4

No special protection against penetrating moisture (IP = International Protection)

ROBERT RIELE GmbH & Co KG Page 3 GENERAL NOTES

Spillings and cleaning

ROBERT RIELE GmbH & Co KG Page 4 GENERAL NOTES

WASTE MANAGEMENT NOTE

Address of the manufacturer:

ROBERT RIELE GmbH & Co KG

Phone: +49 (0)30 404 40 87

QUALITY MANAGEMENT SYSTEM

ROBERT RIELE GmbH & Co KG Page 5 GENERAL NOTES

1 INTRODUCTION TO PHOTOMETER 5010 ................................................8

3 OPERATING ELEMENTS ...........................................................................11

4 SELECTION OF MEASURING PROGRAMS ...........................................16

6 USER GUIDANCE AT DISPLAY ...............................................................41

ROBERT RIELE GmbH & Co KG Page 6 CONTENTS

8 UTILITY PROGRAMS .................................................................................47

10 ERROR MESSAGE / CORRECTION ........................................................58

11 TECHNICAL DATA .....................................................................................61

12 ACCESSORIES AND SPARE PARTS.........................................................66

ROBERT RIELE GmbH & Co KG Page 7 CONTENTS

1 INTRODUCTION TO PHOTOMETER 5010

ROBERT RIELE GmbH & Co KG Page 8 1 - INTRODUCTION TO PHOTOMETER

2.2 PREPARATION FOR INSTALLATION

(c) ROBERT RIELE After switching on Copyright, ...

(c) ROBERT RIELE Website, ...

ROBERT RIELE GmbH & Co KG Page 9 2 - INSTALLATION

2.4 LOADING PRINTER PAPER

ROBERT RIELE GmbH & Co KG Page 10 2 - INSTALLATION

display printer cover

ROBERT RIELE GmbH & Co KG Page 11 3 - OPERATING ELEMENTS

3.3 WORKING AREA

waste tubing pump tube with joints

outlet tube cuvette

joint inlet tube cuvette

joint inlet tube cuvette pump tube with joints

outlet tube cuvette

ROBERT RIELE GmbH & Co KG Page 12 3 - OPERATING ELEMENTS

3.4 FUNCTIONS OF THE KEYS

[0]...[9], [•] Keys for numeric input 5.5/ 7/ 8

[MODE] [4] Printer ON / OFF 5.6

TEXT EDITOR LEVEL

[7 / ABCD] Input of A...B...C...D 7

ROBERT RIELE GmbH & Co KG Page 13 3 - OPERATING ELEMENTS

3.7 CUVETTES AND CUVETTE ADAPTOR

3.7.1 Changing cuvette adaptor

ROBERT RIELE GmbH & Co KG Page 14 3 - OPERATING ELEMENTS

3.7.2 Working with original flow-through system

pump tube with joints

3.7.3 Working with single cuvettes

3.7.4 Working with discrete flow-through cuvette

ROBERT RIELE GmbH & Co KG Page 15 3 - OPERATING ELEMENTS

4 SELECTION OF MEASURING PROGRAMS

No. Program group Chapter Description

1 ... 250 Direct method selection 5 MEASUREMENT WITH BASIC METHODS

[MODE] Utilities 8 UTILITY PROGRAMS

CP-No. ) Description) Characteristic) Method Calculation formula) Factor RB