Souvenir Life Science 250 CDs PDF

JNANARJANA 2K14, 6 th National Scinece Conference on “Beyond the frontiers in Science & Technology”
The Oxford College of Science, Bangalore 1

ABOUT BANGALORE CITY

Bangalore is the capital and the largest city of Karnataka state. It is also called "Garden
City" for its beautiful gardens, flowers and trees which flourish in each and every street. It
is India's fifth largest city and India's fifth largest metropolitan area, with a population of
about 9 millions. Within the last decade, the establishment and success of high technology
firms in Bangalore have lead to the growth of Information Technology (IT) and
Biotechnology (BT) in India. IT and BT firms in Bangalore employ about 30% of India's
pool of 1 million IT and BT professionals. Bangalore is the scientific hub of India and it has
the world renowned and the oldest Research Institutes and Universities that includes
Indian Institute of Science, the Indian Institute of Astrophysics, the Raman Research
Institute, the Jawaharlal Nehru Centre for Advanced Scientific Research, the National
Center for Biological Science, the Indian Statistical Institute and Indian Institute of
Management etc. There are numerous gardens and historical sites within the city to keep
you occupied. The Vidhan Soudha is the prime attraction. The Government Museum of
Bangalore and the Vishvesvaraya Technological and Industrial Museum are worth visiting.
The Lal Bagh Botanical Gardens, holds a number of flower shows, especially during the
Republic Day attracts several tourists. The Fort and Palace of Tipu Sultan are other
important places in Bangalore. You may pray at the temple dedicated to Nandi, the Bull
besides the Venkataramanaswamy Temple, the Gavi Gangadhareswara Cave Temple, the
Someshwara Temple and iskon temple. The Bannerghatta National Park and Ulsoor Lake
are ideal spots to visit. Bangalore is situated in the Deccan Plateau, with an average
elevation of 920 m above sea level. Due to its elevation Bangalore enjoys a pleasant and
equable climate throughout the year. Climatic condition of Bangalore during winter
(November to February) is the coolest period with temperature falling between 10 – 15 °C.
ABOUT THE CONFERENCE

Science is not complicated!! The best scientific attitude about life simplifies things.
Convergence is a new paradigm that can yield critical advances in a broad array of sectors,
from health care to energy, food, climate, and water. The 6th annual science conference
“Beyond the Frontiers in Science & Technology” aims at the convergence of life science and
physical science at one platform for many. It is an intensive two-day conference designed
for the faculty and students from all scientific disciplines. The major objective of this event
is to develop the interest of young researchers for undertaking studies and research in the
frontier fields of physical, chemical and biological sciences and to present their research
proceedings. The event provides a platform for interdisciplinary communication and
intensifies collaborations between academia and industry.
Jnanarjana 2K14, 6th National Science Conference on “Beyond the Frontiers in Science and
Technology” to be conducted on 27th and 28th March 2014, is therefore organized to give
the students a firsthand comprehensive insight into the field of Science and Technology
from renowned speakers from various backgrounds. This conference is hosted by the
departments of Physical, Chemical, Mathematical and Life Science, The Oxford College of
Science, Bangalore.
Jnana, the knowledge is eternal and grows with sharing. Jnanarjana 2K14 aims at
empowering students and faculty with the thoughts and knowledge from eminent speakers
from the Academia as well as industry. This conference will provide a platform for the
young minds from academia, Research & Development and Industry to interact and share
their views with a more open and free mind so as to develop a better perception about the
industrial requirements. The conference is spread over two days – Day 1 will comprise of
Keynote address and technical session in the field of Life science and competitions for
Physical and Mathematical sciences. Day 2 will consist of technical sessions Physical,
Chemical and Mathematical sciences and competitions for Lifescience to make the
conference more interactive. The focal points during both the days would be the technical
sessions by eminent speakers from both academia and industry.
We believe that this conference would truly be an excellent opportunity for all the 800
participants from all over India to imbibe knowledge and take back memorable
experiences with a more clear and focused mind at the end of the day.

ABOUT THE OXFORD COLLEGE OF SCIENCE

The Oxford Educational Institutions under the aegis of Children’s Education Society was
founded by Sri S. Narasa Raju, an eminent educationist of national stature in the year 1974,
with two teachers and twenty four students. The Oxford College of Science is a part of the
thirty institutions under the umbrella of Children’s Education Society. The Oxford College
of Science offers 12 Postgraduate and 12 Undergraduate programmes. Department of
Microbiology, Biotechnology, Biochemistry, Applied Genetics, Chemistry, Electronics,
Mathematics and Computer Science comprise the PG department of life science, Chemical
Science, Physical and Mathematical science delivering high quality education and research
with highly qualified and dedicated teaching faculty. The college is accredited with “A”
grade by NAAC and certified by International Accreditation Organization (IAO) and
also recognized with 2f and 2‘B’ by UGC. Departments are equipped with world class
infrastructure and various government sponsored projects worth around 2 crores. The
Oxford College of Science has consistently secured a place among the top five Science
Colleges in the city of Bangalore for five years since 2007.The Department of Biotechnology
is ranked 8th in India and 1st in Karnataka among the private institutions.
CAMPUS AT A GLANCE
• Total Campus area of 2.5 acres, total plinth area of 2.85 lakh sq. ft.,
• Playground of 5 acres.
• The auditorium with a 650 seating capacity to enable the students and faculty to
organize symposia, conferences and other academic and cultural programs. The
auditorium is well designed with video conferencing facilities to interact with well
known personalities in related fields all over the world.
• World-class classrooms with interactive digital boards, scientific laboratories
designed with imported interiors and furnishings from well known designers and
fabricators.
• Well stocked spacious library with books, scientific journals, e-library facility and
audio visual aids
• Sophisticated computer laboratory with internet facility/Wi Fi Connectivity

• The gallery classrooms are well equipped with latest teaching aids and audiovisual
facilities.
• College automation- SMART campus software with
 Student data-attendance, time table, internal marks, Library book
issue etc.
 Staff data- work load, lesson plan, staff attendance etc.
Biometric system for recording entry and exit time of staff .

Estd. 1974 Ph: 26630855,

22452500- 04 (5 lines),
Fax: 26548658
CHILDREN’S EDUCATION SOCIETY (Regd.)

Regd. No. 284/74-75
THE OXFORD EDUCATIONAL INSTITUTIONS
(Recognised by Govt. of Karnataka)
1st Phase, J.P.Nagar, Bangalore – 560 078
CHAIRMAN’S MESSAGE
It gives me immense pleasure to acknowledge Jnanarjana 2K14, 6 th

National Science Conference on “Beyond the Frontiers in Science and
Technology”, an inventive venture of The Oxford College of Science for
the past six years.
Convergence of all the fields of science on a single platform for the

benefit of many is the key objective of Jnanarjana 2K14. The topics chosen under Life
Science, Physical Science & Mathematical Science encompass the latest trends and advance
techniques in the respective fields. Although crystallography underpins all of the sciences
today, it remains relatively unknown to the general public. In this regard a special lecture
has been arranged on Crystallography.
This conference in true sense will be able to stimulate the young minds, ensuring better
and brighter citizens of rising India. The aim of education is not only to impart knowledge
but to add value to the education and equip all individuals for life and living.
I wish all the success to the team of Jnanarjana 2K14 and the participating delegates. May
God bless one and all.
S.NARASA RAJU
Chairman

Estd. 1974 Ph: 26630855,
22452500- 04 (5 lines),
Fax: 26548658

Regd. No. 284/74-75
THE OXFORD EDUCATIONAL INSTITUTIONS
(Recognised by Govt. of Karnataka)
1st Phase, J.P.Nagar, Bangalore – 560 078
DIRECTOR’S MESSAGE
On the occasion of the 6th ‘Jnanarjana’, National Conference on

“Beyond the frontiers in Science and Technology”, I congratulate
The Oxford College of Science for expanding the scope of the
conference from life sciences to all sciences. This year’s
conference promises to be meaningful as resource talks from
eminent professionals from academic as well as industry would
engage all participants in intellectually charged deliberation. The competitions and
paper presentation sessions too promise to stimulate the creativity and intellect of
the participants.
I am very delighted to note that the organizing committee is bringing out the
conference proceedings. I wish the effort all success.
May the College of Science create more milestones that will lead towards the
establishment of a strong research base.
S.N.V.L. NARASIMHA RAJU

Director

Estd. 1974 Ph: 26630855,

22452500- 04 (5 lines),
Fax: 26548658

Regd. No. 284/74-75
THE OXFORD COLLEGE OF SCIENCE
Accredited ‘A’ Grade by NAAC, Certified by IAO,
(Recognised by Govt. of Karnataka, Affiliated to Bangalore University)
19th Main, Sector IV, HSR Layout, Bangalore 560 102
PRINCIPAL’S MESSAGE
I congratulate the faculty and students of The Oxford College of Science

for convening the 6th ‘Jnanarjana’, 2K14 on the 27th and 28th March
2014. In the previous years jnanarjana centered around life sciences.
This year the inclusive scope of the conference opens to all the
disciplines of the sciences to bring together on a single platform the
scientific community- from academic as well as industry-to deliberate
on the challenging theme of “Beyond the frontiers in Science and Technology”. I am happy
to note that the college is bringing out the proceedings of the conference. I wish the
conference all success and may the college move from strength to strength in research
activities.
Dr.SHARADA AJIT
Principal

ORGANIZING COMMITTEE
Shri.S. Narasa Raju,
Chairman, Children’s Education Society.
Chief Patrons
Shri. S.N.V.L. Narasimha Raju,
Executive Director, The Oxford Eductional Institutions.
Patron Dr.Sharada Ajit, Principal
Organizing Secretary Dr.Bharathi.S, Vice Principal-Academics.
Convener Prof.Gayathri Sudir, Vice Principal-Administration.
Treasurer Dr.Sangita Roy, HOD Biochemistry
Major. Mudde Gowda, Member Governing Council.
Dr.Rajasekaraiah, Director, TOCE.
Advisory
Dr.Nandeshwar. R.L , Director, TOCS & TOCM
Dr.Elsamma Thomas, PG Co-ordinator, TOCS.
Ms. Shanthala.V. S – HOD, Physics.
Ms. Chetana. S – HOD Biotechnology.
Ms. Pavana Kamath.P – Coordinator, Genetics.
Faculty Members Dr. Narayana Swamy – HOD, FAD.
Dr. Anil Kumar. K – HOD, Statistics.
Dr. Venkatesh. V – Coordinator, Chemistry
Dr. Meenakumari. J – HOD, Computer Science
Mr. Ibrahim - Microbiology
Ms. Umang Prajapathi - Electronics
Mr. Aditya Rao - Biochemistry
Student Members
Mr. Deekshit - Biotechnology
Ms. Kavya Hegde - Mathematics
Ms. Chitra Krishnakumar - Genetics

FACILITATION COMMITTEE
COMMITTEE IN-CHARGE MEMBERS
Programme Committee Ms.Vaidhehi Jois , Ms. Deepti Varier & Ms.Muthamma
Registration Committee Mr Prajeesh P, Ms. Sonhita & Ms Asha Ninan
Speaker Hospitality Committee Ms Madukala , Ms Vatsalya Krupa, Ms.Pallavi

Ms.Reka.G, Ms.Iveen Pinky
Stage Committee Ms Vijaya, Ms.Mamata, Ms Deepa Kumari, Ms

Fathimunnisa, Ms.Prathibha Nair
Ms.Kriti, Ms Priyanka, Ms.Smitha.N, Ms.Priya Chacko
Delegate hospitality Committee Ms.Ranjitha
Accommodation Committee Dr.Prakash, Mr.Nagaraju, Dr.Arpita, Ms.Lakshmi Priya,

Ms.Devika Bhai
Publicity & Transportation Mr.Bhanu Prakash, Mr.Badrinath, Mr.Shivaji
Committee Ms.Hemamalini
Media Committee Mr.Yogesh, Dr Neha, Ms Aditi, Ms.Ramandeep Kaur
Food Committee Dr Narayana Swami, Mr Veerendra, Ms.Indu, Ms.Swetha,

Ms.Pavithra
Printing Committee Mr Sekar.K.V & Dr.Pramod T
Cultural Program Committee Ms. Pavana, Ms. Gayathri
Scientific Committee Dr.Abdul Nayeem, Mr.Ramanuja H S, Mr.Prajeesh.P,

Mr.Sekar.K.V
Ms.Chetana Heroor, Ms.Pavana, Ms.Shantala, Ms.Sathya

Event Committee Veena, Ms.Deepthi.D, Ms.Garima
Ms.Chithra, Ms.Raji Sukumar
Mr.Ravichandra, Ms.Bharthi, Ms.Akshata, Ms.Thejaswini,
Technical Committee
Ms.Spoorthy
Ms.Gayathri Sudhir, Dr.Sangita Roy, Ms.Chetana,
Sponsorship Committee
Dr.Narayana Swamy

CONTENTS
ORAL PRESENTATIONS
Abstract No. Title of Abstract

Impact of Cigarette Smoke on Human Peripheral Blood Lymphocytes –
OP-LS-01
Effect of Ascorbic Acid Supplementation and Its Immunological
Significance
OP-LS-02
Protein Profile and Physico-Chemical Properties of Honey
OP-LS-03 Anti-Invasive, Anti-Angiogenic and Pro-Apoptotic Potential of Embelin
Against Human Colon Carcinoma Cell Line- Ht-29.
OP-LS-04 Fabrication and Antipseudomonal Evaluation of Plant Extract Loaded
Chitosan Sponges for Prospective Biomedical Applications.
OP-LS-05 Etiology Of Organisms In ICU in Tertiary Care Hospital and Its Antibiotic
Sensitivity Profile
OP-LS-06 Bioprospecting For Enzyme Producing Attributes of Gut Microbiota of
Rhinoceros Beetle (Oryctes Rhinoceros)
OP-LS-07 Screening, Optimization and Characterization of Poly Hydroxy Alkanoates
(Pha) Produced from Microbial Isolates
OP-LS-08
Evaluation of Anti-Inflammatory Activity of A Poly Herbal Formulation
OP-LS-09 Production and Optimization of Cellulase Enzyme by Aspergillus Niger
Using Pineapple Peel as Substrate
OP-LS-10 Bioremediation of Copper And Zinc from Automobile Effluents by Using
Fungal Flora
OP-LS-11
Biochemical Changes During Aluminium Stress in Amaranthus Tricolor L.
OP-LS-12 In-Vitro and In-Situ Studies on Microbial Detoxication of Plastic Waste from
the Dumping Sites of Bangalore City.
OP-LS-13 Microbial Utilization of Diary Waste for the Production of Value Added
Product – Lactic Acid.
OP-LS-14 Biofabrication of Zinc Oxide Nanoparticles using Macrophomina Phaseolina
and its Antimicrobial Potential.
OP-LS-15
Validation of Test Methods in Microbiology in a Neuro Care Centre.
OP-LS-16
Actinomycetes Producing Multiple Enzymes from Western Ghat Soil.
Anti-Cancer Activity of Methanol Extracts of the Latex of Michelia
OP-LS-17
Champaca and Calotropis Gigantea using Human Breast Cancer Cell Line Zr-
75-1 and Human Leukemia Cell Line Hl60.

OP-LS-18 Prophage Mediated Sero-Divergence in Salmonella Species.

OP-LS-19 A Field Study on Complex Inheritance Pattern Of Diabetes Mellitus
Comparative Morphometric analysis Of Different Species of Termites

OP-LS-20
(Isoptera) from Bangalore
Studies on Low Cost Medium in Production of Chlorella Species Using Cow

OP-LS-21
Urine for the Production of Bio-Diesel
Antiquorum Sensing Activity of Quercetin against Multidrug Resistant

OP-LS-22
Pseudomonas Aeruginosa
Isolation of Oil Degrading Microorganisms from Soil Sample Collected From
OP-LS-23
Oil Spilled Areas of Garages.
Hisotpathological Studies on Hypophysis and Ovary of Fresh Water Fish
OP-LS-24
Glossogobius Giuris (Hamilton)
Production of Streptomycin From Streptomyces Griseus by Solid State
OP-LS-25 Fermentation & Its Production Enhancement by Mutation & Analysis by
HPLC
OP-LS-26 In Vitro Qualitative and Quantitative Phytochemical Studies of Jasmine

CONTENTS
POSTER PRESENTATIONS
Abstract No. Title of Abstract
PP-LS-01 Diversity of Fungi on Some Selected Spices from Bangalore Rural Dist
Leaf Litter Mycoflora of Some Medicinal Plants from Bangalore Rural

PP-LS-02
Dist
Molecular Diversity Of Mushrooms in And Around
PP-LS-03
Bangalore Rural Dist , Karnataka.
PP-LS-04 Microbial Quality Analysis of Locally Available Herbal Products
Studies on Amylase Production from Aspergillus Oryzae by
PP-LS-05
Solid-State Fermentation
Effect of Use Of Different Low Calorie Intense Sweeteners and Overall
PP-LS-06
Quality of Yoghurt, Bioyoghurt and Probiotic Mishti Dahi.
Comparative Study of Anti-Oxidant and Anti-Diabetic Activity of
PP-LS-07
Kigelia Pinnata
Biosorption of Zinc by Heavy Metal Resistant Fungi Isolated from
PP-LS-08
Bellandur Lake
Alternative Method of Extraction of Eugenol from Syzygium
PP-LS-09
Aromaticum and its Applications
In-Vitro and In-Situ Studies on Microbial Detoxication of Plastic Waste
PP-LS-10
from the Dumping Sites of Bangalore City
Comparative Evaluation of Three Medicinal Plants for Antibacterial
PP-LS-11
Activity against Important Species Of Bacteria.
Hydroxyl Radical Scavenging and 1, 1-Diphenyl-2-Picrylhydrazyl
PP-LS-12
(DPPH) Radical Scavenging Activity Of Psoralea Corylifolia L .
PP-LS-13 Production of Biodiesel from Microalgae, Edible and Non-Edible Oils
Effect of Culture Filtrate of Trichoderma Species against Important
PP-LS-14
Seed Borne Fungal Pathogens of Paddy
Solid Waste Treatment and its Conversion to an Eco-Friendly Product
PP-LS-15
by Effective Microorganism (EM) Technology
Study on the Effect of Salon Wastes on Human Health and a Novel Way
PP-LS-16
to Utilize the Waste Hairs
PP-LS-17 Antibacterial Studies Of Caesalpinia Bonducella Seed Extracts

ELISA- Best Advanced Handy Detection Test of Picking the

PP-LS-18 Specific Progesterone Metabolite in Fecal Samples of Wild
Asian Elephants
Effect of Aqueous and Solvent Extract of Piper Longum L.
PP-LS-19
Root against Important Species of Bacteria
Effect of Interaction of Soluble Starch and Stabilizer on the
PP-LS-20
Growth Behaviour of Lactic Acid Bacteria
Bactericidal, Fungicidal and Anthelmintic Activities of
PP-LS-21
Alstonia Scholaris Bark Extracts
PP-LS-22 Isolation, Purification and Characterization of Protease
PP-LS-23 Novel Diagnostic Method for Tuberculosis
Evaluation of Formononetin and Biochanin a as
PP-LS-24 Selectiveestrogen Receptor Modulators (SERM) - Potential
Implications in The Therapy of Post-Menopausal
Osteoporosis
Phenotypic and Molecular Evaluation of F 7 Tomato RIL’s for
PP-LS-25
Extended Shelf Life
PP-LS-26 Screening of Streptomyces Spp for Lipase Inhibitors
Oral Pathogens in Hiv Infected Patients with Special
PP-LS-27
Reference to Candida
Protective Effects of Resveratrol against Β-Adrenergic
PP-LS-28
Agonist Induced Myocardial Stress in Diabetic Rats
Couroupita Guianensis Flower Extract (Nagalinga Pushpa): A
PP-LS-29
Potential Natural Remedies for Dental Caries
PP-LS-30 Isolation and Screening of Heavy Metal Resistant Bacteria
Seroprevalence Survey of Rubella Infection Among Females
PP-LS-31
of Reproducing Age (15-40) Years Atytending Rims, Ranchi
Agro Waste As Source of Eco-Friendly Natural Dye For Silk
PP-LS-32
Antidermatophytic Properties of Turmeric Oil, and Curcuma
PP-LS-33 longa Preparations

Oral
presentation

OP-LS-01
IMPACT OF CIGARETTE SMOKE ON HUMAN PERIPHERAL BLOOD LYMPHOCYTES –

EFFECT OF ASCORBIC ACID SUPPLEMENTATION AND ITS IMMUNOLOGICAL
SIGNIFICANCE
Anupama Singh, Uthayasuriya, Anjali , Bhavya and Sreepriya M *

*Department of Microbiology & Biotechnology
Bangalore University, Bangalore – 560 056
ABSTRACT
Cigarette smoking has been implicated as a predisposing factor in several human diseases
including cardiovascular disease, malignancy and other inflammatory diseases of the
airway and lungs. Smoking is also reported to suppress the immune responses leading to
the increased susceptibility of smokers (both active and passive) to microbial infections.
Antioxidant supplementation in vivo has been found to negate cigarette smoke(CS) –
induced pathological and biochemical changes in animal models. The current study was
designed to understand the effects of supplementation of the antioxidant ascorbic acid to
prevent cigarette smoke-induced cytotoxicity and alterations in cell morphology. As
lymphocytes are the principal mediators of immune response, human peripheral blood
lymphocytes were used as the model systems in the study. Cell viability was evaluated by
MTT assay and cell morphology by phase contrast microscopy analysis. Results indicated
that exposure of human peripheral blood lymphocytes to cigarette smoke for about 7.5
seconds resulted in severe cellular damage and cytotoxicity. Pretreatment of the cells with
ascorbic acid at a concentration of 10μg/ml considerably protected against CS-induced
cytotoxicity. Also, it was observed that post treatment of the cells with 10μg/ml of ascorbic
acid after CS exposure did not protect the lymphocytes against damage and a higher
concentration of 30μg/ml and above was required to observe the protective effects. Results
implicate the immunosuppressive effects of CS in vitro and the beneficial effects of ascorbic
acid supplementation to combat CS-induced damage in human peripheral blood
lymphocytes and thereby its role in boosting up the immunity of an individual.
Key words: Cigarette smoke, lymphocytes, ascorbic acid, MTT assay, cytotoxicity

OP-LS-02
PROTEIN PROFILE AND PHYSICO-CHEMICAL PROPERTIES FO HONEY
Jyothi S. C., Akshatha Avadhani, Surendra N. S.

Department of Biological Sciences, Bangalore University, Bangalore.
ABSTRACT
Honey is the yellow color liquid produced by honeybees from the nectar of blossoms,
sucking insects living on parts of plants. When the nectar is passed from bee to bee before
being stored in the cells of the hive, more secretions from the salivary and hypo-pharyngeal
glands of foraging bees are added enabling the nectar to ripen into honey. Honey is a
complex mixture and presents very great variation in composition and characteristics due
to its production by different bee species during different seasons with different floral
composition by differing harvesting and post harvesting techniques, all of which greatly
influence the quality of honey. The genus Apis includes 7 recognized species of honey bees
of which 4 are major groups & the other 3 are minor species. The major species include 2
hive bees’ namely A. mellifera & A. cerana. The widely available and commercially used
honey is obtained from A. cerana.
The present study reveals that there is a significant variation with respect to physico-
chemical properties such as pH, total dissolved solids (TDS), conductivity, density,
viscosity, moisture content, optical density (OD), total carbohydrates, total reducing sugars,
glucose % by mass, fructose % by mass, laevulose/ dextrose ratio, protein content, catalase
activity, total phenolics, flavonoids, ascorbic acid, acid value and protein profile of
unprocessed hive honey. This when compared with commercial/branded honey samples
also showed the significant variations. These variations are due to biotic and abiotic
factors. The degrees of variation between the raw and commercial honey is due to
processing of honey. The quality of commercial honey can be maintained by improved
processing technology and variations, which are seen among the raw honey. The T-test
values with respect to the various raw honey samples were obtained from the beekeepers
in different natural geographical regions of Karnataka. The sensory characteristics of the
honey, of different regions of Karnataka are described together with physico-chemical and
protein profiling analysis. However, this kind of studies are further required in various

other parts of the state, in specific and at large, the nation. This helps to understand the
physico-chemical properties, protein profile and other parameters of honey needed for
further understanding.
OP-LS-03
ANTI-INVASIVE, ANTI-ANGIOGENIC AND PRO-APOPTOTIC POTENTIAL OF EMBELIN
AGAINST HUMAN COLON CARCINOMA CELL LINE- HT-29.
Sumalatha.K.R and M.Sreepriya*

*Department of Microbiology and Biotechnology
Bangalore University, JnanaBharathi Campus
Bangalore 560 056, Karnataka, India
ABSTRACT
Colon cancer is the third most common cancer in men and the second in women
worldwide. Decades of expensive and replicating research studies have had little impact on
primary prevention. Our previous findings demonstrated the growth inhibitory effects of
embelin in human colon cancer cell line HT-29. In the present study, we aimed to further
examine the potential of embelin in controlling angiogenesis and tumor cell invasiveness,
proliferation and pro-apoptotic potential against invasive human colon adenocarcinoma
cell line HT-29.
A battery of relevant and powerful short term in vitro assays were conducted to check for
the effects of embelin on cell proliferation (Sulphorhodamine B assay , BrdU
incorporation test), Pro apoptotic effects (TUNEL assay, COMET assay, Annexin-FITC
labeling and DNA fragmentation assay) anti-invasive effect (Boyden chamber assay and
scratch assay) and angiogenesis ( in vitro angiogenesis assay). Influence of embelin on
the expression of p53, Caspase 3 and 9, Bcl2, XIAP and TNF-α was investigated by reverse
transcription PCR.
A significant decrease in cell proliferation was observed on embelin treated HT-29 cells
coupled with a drastic reduction in the number of capillary like structures formation on
matrigel coated plates implicating the effect of embelin on cell migration and invasion.
Increased number of comets, phosphatidyl serine externalization, increased expression of

p53, Caspase 3 and 9 with increased expression of Bcl2, XIAP and TNF-α was observed
after treatment with embelin thereby confirming its proapoptotic effects.
Conclusion: Embelin inhibits angiogenesis, cell invasion and proliferation. It was also
observed that embelin is selectively cytotoxic on carcinoma cells as opposed to normal
cells. This is hypothesized to be partly mediated by upregulation of the p53 signalling
pathway and its inhibitory effects on XIAP. Current study supports its therapeutic use in
the treatment of advanced and metastatic colon cancer.
OP-LS-04
FABRICATION AND ANTIPSEUDOMONAL EVALUATION OF PLANT EXTRACT LOADED

CHITOSAN SPONGES FOR PROSPECTIVE BIOMEDICAL APPLICATIONS
Adheena K.S 1, Ibrahim V 1, Asha ninan 1, Jayachandran V.P 2

1. PG Department of microbiology, The Oxford College of Science.
2. Pushpagiri Research Centre, Pushpagiri Institute of Medical Sciences, Tiruvalla
ABSTRACT
Chitosan (CH) is a natural mucopolysaccharide of marine origin consists of a linear (1-4)

linked 2-amino-2-deoxy-D glucan, can be chemically prepared from naturally occurring
chitin by treatment with alkali at elevated temperature. CH is non-toxic, biodegradable and
biocompatible polymer possessing antibacterial, heavy metal adsorption, antioxidation
and film forming properties. The cationic properties of CH offer the added advantage of
electrostatic interactions with other anionic polysaccharides. It complex with negatively
charged moieties such as sodium carboxymethylcellulose, citrates, pectin, acacia, agar,
sodium caprylate, stearic acid, gluteraldehyde, sodium tri-polyphosphate, lactic acid, malic
acid and alginic acid. In addition CH acts as a penetration enhancer by opening the tight
epithelial junctions and this property of CH underlines the futuristic applications in protein
and vaccine delivery.
The prime objective of this study is the development and characterization of CH
sponge loaded with plant extracts as well as the evaluation of the antibacterial potential.
Various Sponges were prepared by dissolving CH in (2%) acetic acid with different

concentrations of CH (4% 6% 10%). On freeze drying, using more concentrated CH

enhanced pore density of the sponge. The sponge prepared was cross-linked with sodium
triphosphate solution and the morphology of CH sponges were characterized under a
scanning electron microscope (SEM). The sponges were loaded with selected plant
extracts to study its antibacterial activity. The water sorption capacity was checked by
weighing the CH sponge (W1) and then immersed in phosphate buffer (pH 7.4; immersion
medium), sponge remnants were wiped off excess surface water using filer paper and
weighed (W2) at different time intervals. The immersed sponges were dried at 60°C for 24h
and kept in a desiccator for 48h prior to reweigh (W3). The water sorption was calculated
using the following formula: %water sorption = (W2-W3)/W3x100.
Aqueous extract of Punica granatum, was prepared using decoction method and on
filteration, centrifugation the extract was dried using vacuum evaporator to obtain a
residue of crude extract. The sustainable antibacterial activity of drug loaded sponge was
assessed using agar diffusion method. Sustainable antibacterial activity of developed
sponge was evident against Pseudomonas aeruginosa and can be utilized as a therapeutic
strategy against bone infections after clinical trials.
Key Words: Multiple drug resistant Pseudomonas aeruginosa (MDR PA), Punica granatum,
CH-chitosan, SEM, FT-IR analysis.
OP-LS-05
ETIOLOGY OF ORGANISMS IN ICU IN TERTIARY CARE HOSPITAL AND ITS ANTIBIOTIC

SENSITIVITY PROFILE
Ahuti Debasmita, Amrita Raj, Basim Sufi, Prajeesh P

Department of Microbiology, The Oxford College of Science, Bangalore-560102
ABSTRACT
Medical microbiology is the study of the interactions between animals (primarily humans)
and microorganisms such as bacteria, viruses, fungi, and parasites. Although the primary
interest is in diseases caused by these interactions, it must also be appreciated that
microorganisms play a critical role in human survival. The microbial flora in and on the

human body is in a continual state of flux determined by a variety of factors, such as age,
diet, hormonal state, health, and personal hygiene. An understanding of medical
microbiology requires knowledge not only of the different classes of microbes but also of
their propensity for causing disease. Nosocomial infection is defined as an infection which
develops 48 hours after hospital admission or within 48 hours after being discharged. The
objectives were to assess the frequency of nosocomial infection in patients admitted to
intensive care unit (ICU) and to determine the etiological factors in such patients. Bacteria
were isolated from most of the samples. Relevant samples from patients suspected to have
infection were cultured and sensitivity testing was performed according to standard
procedures. Members of the family Enterobacteriaceae are among the most important
bacterial human pathogens accounting for the majority of bacteria isolated from clinical
sample. The most frequent site of infection was respiratory tract (23%) and urinary tract
(23%). Pseudomonas (P.), Klebsiella (K.), Escherichia (E.) coli, Acinetobacter and Candida
spp. were the commonest organisms. The isolation rate of Gram-positive bacteria was
relatively low. Majority (> 50%) of the Gram-negative isolates were resistant to many of
the antibiotics tested. Relatively low resistance was only observed against Tobramycin.
Majority of Gram-negative isolates were resistant to Ceftizoxime and Ciprofloxacin. The
high frequency of HAIs and antibiotic resistance rate, suggests that more strict infection
control practices along with prescription of antibiotics after antibiotic susceptibility testing
should be implemented to limit the emergence of antibiotic resistant organisms. Patients
admitted in intensive care unit are at more risk of acquiring nosocomial infection from
different sources. It is suggested that proper nursing care, sterilization and disinfection of
instruments and equipment and careful handling of invasive procedures are the best tool to
control these life threatening infections.

OP-LS-06
BIOPROSPECTING FOR ENZYME PRODUCING ATTRIBUTES OF GUT MICROBIOTA OF

RHINOCEROS BEETLE (Oryctes rhinoceros)
Dr. R. Rangeshwaran, Akshatha Avadhani1, Frenita Maria Lewis and Ashwitha K

National Bureau of Agriculturally Important Insects (ICAR),
P.B.No. 2491, H A Farm Post, Bellary Road, Hebbal, Bangalore 560024
1Department of Biological Sciences, Bangalore University, Bangalore
ABSTRACT
The current study aimed to isolate amylolytic and cellulolytic bacteria associated with the
gut of Oryctes rhinoceros. These insects are major pests of coconut, oil and other palms but
they contribute to important ecological functions such as nutrient cycling. Freshly cultured
larvae of Oryctes rhinoceros were used to isolate and identify the micro organisms
associated with them. The microbes were cultured from the gut of the samples on nutrient
agar. Total of 9 bacterial colonies were isolated. DNA was extracted and nearly full-length
bacterial 16S rDNA fragments were amplified by polymerase chain reaction (PCR) from
each representative bacterial isolates. The PCR products were sequenced, and the
sequences were aligned against those sequences found in the NCBI database using the
BLAST (Basic Local Alignment and Search Tool) algorithm. The analysis revealed that the
gut of larvae Oryctes rhinoceros contain a complex gut microbiota including Lysinibacillus
sphaericus, Bacillus subtilis, Bacillus amyloliquifaciens, Bacillus megeterium, Bacillus
methylotrophicus, Bacillus altitudinus, Bacillus tequilensis, Staphlococcus saprophyticus. To
determine amylolytic and cellulolytic activity, starch agar medium and
carboxymethylcellulose(CMC) medium were used respectively. The activity was indicated
based on intensity of clear zone formation in the media. The results showed that B.
amyloliquifaciens had the widest clear zone formation in starch agar medium and CMC
medium indicating highest amylase and cellulolytic activity. Significance of this study
revealed occurrence of microflora in the gut of Oryctes rhinoceros which could suggest a

symbiotic relationship. The data could be used to explain the ability of larval scarabs to
feed on recalcitrant organic matter and exploiting those microbes for industrial purpose.
Key words: Oryctes rhinoceros, 16S rDNA, gut microbiota, amylase, cellulase.
OP-LS-07
SCREENING, OPTIMIZATION AND CHARACTERIZATION OF POLY HYDROXY

ALKANOATES (PHA) PRODUCED FROM MICROBIAL ISOLATES
Amrita Raj, Ibrahim V, Monica Devi, Sekar K V, Yogesh B J, Bharathi S

Department of Microbiology, The Oxford College of Science, Bangalore, Karnataka.
Corresponding Mail: sekar.kv@gmail.com
ABSTRACT
Polyhydroxyalkanoates (PHAs) are polyesters of hydroxyalkanoates synthesized by various

bacteria as intracellular carbon and energy storage compounds and accumulated as
granules in the cytoplasm of cells. PHA producing bacteria from activated sludge and soil
were isolated, characterized and screened by Sudan Black staining method. All the
potential PHA producers were isolated, out of which two of Bacillussp and one of
Pseudomonas sp and it was confirmed by their morphological and biochemical characters.
PHA was extracted directly with solvent chloroform and estimated spectrophotometrically.
Optimization of PHA accumulation was carried out at deifferent temperature. The strain
PHA-1 showed higher amount of PHA accumulation when compared to the other strainsi.e.
52 mg/ml. The world wide dependence on petroleum by products for the manufacturing of
plastics, scarcity of space for disposal and growing environmental concerns over non
biodegradable synthetic plastics have fuelled research towards development of eco friendly
biopolymer material. Hence, attention has been laid on production of Polyhydroxybutyrate,
one of the most extensively studied PHA, is an intracellular microbial thermo plastic that is
widely produced by bacteria present in soil.
Keywords: Poly hydroxy alkanoates, Sudan Black Staining, Granules.

OP-LS-08
EVALUATION OF ANTI-INFLAMMATORY ACTIVITY OF A POLY HERBAL

FORMULATION
K.S.Dayananda1. S.M.Gopinath1 Ismail Sharief.M1. Jagan Mohanreddy.P1 And Aparna

Gopal2
1Department of Biotechnology Engineering Acharya Institute of Technology Bangalore-
560107
2Post graduate student ,Department of Microbiology East west first grade college
Bangalore
ABSTRACT
Medicinal plants cure many diseases associated with inflammation like cardiovascular
diseases, diabetes, cancer and rheumatoid arthritis. In recent years, natural compounds
such as phenolic acids, flavonoids, triterpenes and alkaloids present in various plants, act
as potential anti diabetic and anti-inflammatory agents. The main difficulties in using
natural products as a source for pharmaceutical lead compounds involve separating the
compounds from the crude extracts. In search for novel anti-inflammatory and anti-
diabetic plants are still intensifying. This study, confirms different plant species revealing
anti-inflammatory and anti-diabetic capabilities along with their other medicinal activities.
Peripheral inflammation involves an increase in cyclooxygenase-2 (COX-2)-mediated
prostaglandin (PG) synthesis in the central nervous system (CNS), which contributes to
allodynia and hyperalgesia. In the present study we have determined the changes in
prostanoid tissue levels and in expression of terminal prostanoid synthases in both the CNS
and inflamed peripheral tissue during carrageenan-induced paw inflammation in the rat.
Prostanoid levels were measured by liquid chromatography-mass spectrometry and
enzyme expression at the RNA level by quantitative PCR analysis during both the early (1-6
h) and late (12 and 24 h) phases of the inflammatory response. In the paw, the early phase
was associated with increases in PGE2 and thromboxane (TX)B2 levels and with a peak of
COX-2 expression that preceded that of microsomal prostaglandin-E2 synthase-1 (mPGES-
1). COX-2 and mPGES-1 remained elevated during the late phase, and PGE 2 continued to
further increase through 24 h.. The results show that the carrageenan-induced edema in
the paw elicits an early phase of COX-2 induction in the CNS leading to an increase

synthesis in PGD2, 6-keto-PGF1α, and TXB2 in addition to the major PGE2 response. The data
also indicate that the up-regulation of mPGES-1 contributes to COX-2-mediated PGE2
production in the CNS during peripheral inflammation
Key Words: cyclooxygenase-2, carrageenan, thromboxane, Quantitative PCR.
OP-LS-09
PRODUCTION AND OPTIMIZATION OF CELLULASE ENZYME BY Aspergillus niger

USING PINEAPPLE PEEL AS SUBSTRATE
Abdul Nayeem, Atul Chandra, Ganesha R, Dhondup Namgyal, and Kiran Kumar S
P.G. Department of Biotechnology, The Oxford College of Science,
HSR Layout, Bangalore.
ABSTRACT
The bioconversion of agro-waste based lignocellulosic material to energy has gained much
interest during the recent past. The enzymatic degradation of waste cellulose by fungal
enzymes has been suggested as a feasible alternative for the conversion of lignocellulosic
material into fermentable sugars and ethanol. Cellulose is the principal constituent of the
cell wall of most terrestrial plants. Despite a worldwide and enormous utilization of natural
cellulosic sources, there are still abundant quantities of cellulose-containing raw materials
and waste products that are not exploited or which could be used more efficiently.
Pineapple is a tropical plant and fruit. Its scientific name is Ananas comosus and it belongs
to the family Bromeliaceae. The agro-waste can be effectively utilized for the production
and characterization of cellulases by Aspergillus niger on pineapple agro-waste .
Cellulase production from cellulosic pineapple waste using Aspergillus niger was assessed.
The pineapple peel waste were dried, pre-treated with alkali and re-dried and then
blended. The powdered waste was then used as substrate in separate shake flask which
contain mineral salt medium MSM and inoculi of Aspergillus Niger. Fermentation were
carried out in flask containing the MSM (Mineral Salt Medium), the waste substrate and the
inoculumn at pH 5.0, 1% substrate concentration, 10% inoculum size (1 ml) and cultured
on a rotary shaker (250 rpm) at 29±1˚C initially for 5 days to verify cellulase production by
the organism from the waste substrate, the for 7 days, while varying different fermentation

parameters. Cellulase activity and amount of glucose produced by the test organism’s i.e
from the waste substrate were determined and compared.
The amount of glucose produced was optimized by varying the fermentation parameters:
Time, pH, Substrate concentration, Inoculum size and Temperature. The studies showed
that 5gm substrate at 8th day of fermentation has higher specific activity of the enzyme and
for pH, the optimum is pH 6 on 6 th day, inoculums size is 5ml and temperature is 45˚C.
Though the present study is a small scale process, yet by using the optimum parameters, it
revealed that the following study can help to enhance the production of cellulase. The
study revealed that pineapple peel which is an examples of domestic and industrial
agro-waste , produce large amounts of cellulase enzyme when hydrolysed by cellulolytic
microorganisms and instead of being left behind for natural degradation can be utilized
efficiently under the above mentioned conditions to produce enhanced amount of cellulase
which has massive role in various industries.
OP-LS-10
BIOREMEDIATION OF COPPER AND ZINC FROM AUTOMOBILE EFFLUENTS BY USING

FUNGAL FLORA
B.K.Chikkaswamy and Rabin Chandra paramanik and Prasad M.P

Department of Biotechnology, SRN Adarsh College of Science
5th Main, Chamarajpet, Bangalore-560018
Sigma BioScience Research center, 2 nd stasge, Indiranagar, Bangalore-38
ABSTRACT
The present work was carried out to study the physico-chemical nature of a autobmobile
industry treated and untreated effluent and also to identify the fungi in the samples. For
bioremediation studies, live selected fungal mats and dead powdered fungal mycelia
materials were used for biosorption of copper and zinc contents which revealed there was
a significant absorption of these contents. The results indicated that the dead pretreated
biomass of A. niger reduced copper content in solution by 72.66% followed by the dead
biomass which resulted in 44.86% reduction. Similarly, the dead biomass of P.
ochrochloron and other species reduced copper content in solution by 48.88% followed by

the dead pretreated biomass which resulted in 41.4% reduction. The same results were
obtained in the reduction of zinc content also. The effect of pollutants from diffuse sources
from various industries can be hazardous since most of them contain extremely high levels
of suspended solids, dissolved salts, particulate putrescible organic matter and high pH.
The analysis of treated and untreated effluents from autobmobile industries in Bangalore ,
Karnataka has revealed varied amounts of chemical substances over various durations. The
untreated effluent recorded a higher BOD and COD levels at all duration when compared to
the treated effluent. Further the microflora studied in the untreated effluent recorded the
presence of variety of fungi than in the treated effluent samples.
OP-LS-11
BIOCHEMICAL CHANGES DURING ALUMINIUM STRESS IN Amaranthus tricolor L.
Bhanuprakash KS1, Sumit Jha2, Neeraj Jha2, Moumita Sardar2, Harika.A2, Devika
Mohan2, Prakash Bapodara2, Sangita Roy*
1 Assistant Professor, Department of Biochemistry, The Oxford College of Science,
Bangalore, Karnataka.
2 M.Sc. IV semester Student, Department of Biochemistry, The Oxford College of Science,
th
Bangalore, Karnataka.
* Corresponding author: sangitaroy1973@yahoo.com
ABSTRACT
Environmental contamination of soil by trace metals is a serious problem worldwide due to
their accumulation in food chain. Many plant species are sensitive to micro molar
concentration of aluminum (Al) when present in acidic soil and it is one of the potential
toxic metal for human health. Al induces the oxidative damage in the plants during
accumulation. Plants exhibit some defence mechanism to overcome the stress due to metal
exposure through their antioxidants. In this study, the effect of Al stress on growth and
other related antioxidants and scavenging enzymes were investigated in Amaranthus
tricolor L. Al induced growth retardation observed more in roots compared to shoots. The
antioxidants and enzymes exhibited initial increase and subsequent decrease in response
to Al stress. Accumulation of Al has found predominantly in root compared to shoot.
Key Word: Amaranthus tricolor, Aluminum, Antioxidants, Scavenging enzymes

OP-LS-12
IN-VITRO AND IN-SITU STUDIES ON MICROBIAL DETOXICATION OF PLASTIC WASTE

FROM THE DUMPING SITES OF BANGALORE CITY
Hima CS, Hitha CS, Deepthi Arun Varier and Bharathi S

Department of Microbiology, The Oxford College of Science, Bangalore – 560102
Corresponding E-mail ID – himacs.90@gmail.com
Plastics are synthetic substances produced by chemical reactions. The issue of plastic waste
management is a complex process and there is no simple solution for reducing this waste.
Plastic causes serious damage to the environment, both during its production and disposal.
Biodegradation of plastic waste using microbial strain could offer a solution to this
problem. Microorganisms have been a good resource for solution to this problem due to
their diverse metabolic capability, adaptability to different environment and possibility of
isolation using artificial growth media for utilization in situ.
In the present study, potent microbial strains degrading plastics constituting poly vinyl
chloride (PVC) and low density poly ethylene (LDPE) were isolated using soil burial
technique. To increase the chances of isolating such strains which could have adapted to
metabolize plastic constituting polymers, samples were collected from Mavalipura
dumpsite, Bangalore that was rich in plastic waste. At the end of the study,
Kingellasp.,Actinomycessp,,Alkaligens sp.,Methylobacillussp.was identified. The microbial
species associated with PVC were Kingella sp., Actinomyces sp. and LDPE were Alcaligens
sp., Methylo bacillus sp.The biodegradation of the respective plastics was analyzed for after
2 months, 3 months and 4 months of incubation in invitro condition. The degradation was
obtained as 2% weight loss for PVC sample and 6% weight loss for LDPE after 2 months,
10% weight loss for PVC and 15% weight loss for LDPE after 3 months, 21.3% weight loss
for PVC and 26.1% weight loss for LDPE after 4 months. Optimization methods were
employed to monitor different parameters like pH, temperature and concentration of
plastics for the increased biodegradation of the respective plastics. Optimum pH was found
to pH 7, temperature was found to be 37°C and plastic concentration was found to be

0.50% for both bacterial and fungal isolates identified. The soil sample analyzed for the
Micronutrients, Macronutrients &also heavy metals. The soil sample collected was alkaline .
The electrical conductivity was found to be ideal. Organic matter was deficient.
Phosphorous was also found deficient. Potash level was found ideal. Iron content was high
.Manganese, Zinc and Copper level also found high. Secondary Nutrient and heavy metal
also found low.The Chromosomal DNA isolated from the bacterial isolates and Plasmid
DNA isolation also done and plasmid DNA were isolated from Methylobacillus sp. and
Alcaligens sp. confirmed the degradation capacity reside in the Plasmid DNA in these two
isolates and no plasmid were isolated from Kingella sp. and Actinomyces sp. and confirmed
the degrading gene is located in the chromosomal DNA. The degrading ability of microbial
strains was evaluated by performing colonization studies and sturm test analysis.
Colonization studies on plastic film was performed over a period of one month by
measuring the optical density of the microbial culture. Plastic degradation by the microbial
strains was further evaluated by measuring the CO 2 evolved which was calculated
volumetrically by sturm test. The isolated bacterial strains showed a promising
degradation pattern as analysed by the above mentioned methods.
Keywords: Biodegradation, LDPE and PVC, Sturm test, Plastics.
OP-LS-13
MICROBIAL UTILIZATION OF DIARY WASTE FOR THE PRODUCTION OF VALUE

ADDED PRODUCT – LACTIC ACID
Hitha CS, Hima CS, Sekar KV and Bharathi S

Corresponding E-mail ID – sekar.kv@gmail.com
ABSTRACT
Lactic Acid Bacteria (LAB), which are considered to be the most useful microorganisms to
society, are involved in the manufacture of thousands of fermented foods. They are also
beneficial in flavouring foods, inhibiting pathogenic as well as spoilage bacteria in these
products.The aim of this study was to isolate LAB and optimization of lactic acid production
using whey as carbon source from immobilized Lactic acid bacteria. Lactic acid can be
produced by either microbial fermentation or chemical synthesis. The widely used choice
substrates for lactic acid fermentation are refined sugars, which are inexpensive.Lactic acid
can also be produced from abundantly available cheaper substrate, such as whey. The lactic
acid bacteria were isolated from various sources such as curd, idle dough, pickles and
yoghurt.The identification tests were performed based on their morphological
characteristics and biochemical reactions.Four potential isolates were screenedout for the
production of lactic acid. Immobilized cells of LAB isolated from curd (LAB-C) was found to
produce of maximu amount of Lactic acid (127.8 g/L) followed by LAB isolated from pickle
(118.8 g/L), yoghurt (92.8 g/L) and idle dough (68.0 g/L). This study focuses on the
production of lactic acid from whey by LAB. Whey is a by product of dairy industry which
contains lactose. Maximum lactose conversion to lactic acid was observed with
immobilized cells compared to free cells.
Key Words: Lactic Acid Bacteria, Fermentation, Fermented Foods, Immobilization.
OP-LS-14
BIOFABRICATION OF ZINC OXIDE NANOPARTICLES USING Macrophomina phaseolina
AND ITS ANTIMICROBIAL POTENTIAL
Lakshmeesha T. R1., Priyadarshini K1., Sumanth B1., Nalini Y. S1., Rashmi T. N1.,
Vedashree S1., Shafi sofi Mohammed1 and Sateesh M. K1*, Nagabhushana H2.
1Molecular Diagnostic Laboratory, Department of Microbiology & Biotechnology, Jnana
Bharati Campus, Bangalore University, Bengaluru -560056, India.
2Prof. C.N.R. Rao Centre for Advanced Materials, Tumkur University, Tumkur-572 103,
India.
ABSTRACT
In the present work Zinc Oxide nanoparticles (ZnO NPs) were successfully synthesized
using Macrophomina phaseolina by low temperature combustion method at 400 0C. Zinc
nitrate hexahydrate (Zn (NO3)2.6H2O) was used as a precursor material. The optical
properties of the samples were investigated by UV/vis spectroscopy, Fourier transform
infrared spectroscopy (FTIR), Raman spectroscopy. The structural characteristics were
determined by Scanning Electron Microscopy (SEM), Transmission electron microscopy
(TEM) and Powder X-ray diffraction (P XRD). TEM image shows agreement with the PXRD
data and indicates that the average size of the synthesized nanoparticles were 30 to 50 nm.
Using molecular techniques Macrophomina phaseolina and Cladosporium cladosporioides

were identified. Experiment was conducted to determine the antibaercterial activity by

broth microplate dilution method using 96-well plates against Staphylococcus aureus
(MTCC 96), Pseudomonas aeruginosa (MTCC 429), Escherichia coli (MTCC 443) and Bacillus
cereus (MTCC 432). Subsequently, the antifungal activity was evaluated against soybean
seed-borne fungi Cladosporium cladosporioides by food-poison method. The antimicrobial
activity was found to be enhanced with the increase in concentration of ZnO NPs in dose
dependent manner.
Keywords: ZnO NPs, Macrophomina phaseolina. Cladosporium cladosporioides, molecular

identification.
OP-LS-15
VALIDATION OF TEST METHODS IN MICROBIOLOGY IN A NEURO CARE CENTRE
Latha.G, Nishitha, Sahana.A.V, Sonhita Chel and Ravikumar.R
Department of Microbiology, The Oxford College of Science,Bangalore-560102
Corresponding email id:sonhitachel@gmail.com
ABSTRACT
Validation is the cognitive process of establishing a valid proof. It can also be called as
confirmation or corroboration or the declaration of validity. The process mainly includes
confirmation of reality by comparision .The present study was designed to compare fully
automated methods in Microbiology for its efficiency in rapid and accurate identification
and to determine the percentage of error seen in conventional methods in National
Institute of Mental Health and Neuro Sciences(Department of Neuromicrobiology).
The study consisted of the following samples: Blood(8),Urine(7),Tracheal secretion(9),

Sputum(4), Stool(2), Pus(3), Wound swab(3), Nasal(1), Shunt Tip(2) and 8 other samples.
The various tests included in the study were Gram Staining, Culturing, Identification of
Microorganisms and its Antibiotic Sensitivity Test, Detection of pathogens in blood
samples, Detection of M.tuberculosis. The automated instruments used in the study was
Previ Color Gram, Previ isola, MGIT, BacT/Alert , Vitek 2C60.
From the validation done during the period of two months, 50 different samples were
obtained from patients with different clinical diagnosis and results of various test methods
in Microbiology were analysed and validated. It was observed that automated methods

were much better than conventional methods in giving rapid and more reliable results.
Since both the methods had their own advantage and disadvantage we conclude that
automation was used in tandem to obtain accurate result and to increase the probability of
isolation and identification of bacteria in order to diagnose clinical samples.
OP-LS-16
ACTINOMYCETES PRODUCING MULTIPLE ENZYMES FROM WESTERN GHAT SOIL
Mamatha B Salimath 1, Soumya. S. Rao, Apoorva. M, R. Onkarappa2.

P.G. Department of Microbiology, Dr. C.D.Sagar Research Center for Life Sciences,
Dayananda Sagar Institutions, Kumar Swamy Layout, Bangalore-560078.
P.G. Department of Studies and Research in Microbiology, Sahyadri Science College
(Autonomous), Kuvempu University, Shivamogga-577203
ABSTRACT
Actinomycetes are gram positive bacteria characterized by characterized by the formation

of substrate and aerial mycelium on solid media, presence of spores and a high GC content
of the DNA (60-70 mol %). In nature, biodegradation by actinomycetes plays an extremely
useful role in waste removal and is an integral part of recycling of materials in nature.
Actinomycetes decompose many substrates found in soil and act as scavenger which
explains its potential to use diverse substrates by producing specific enzymes. 20 different
Actinomycetes were isolated from the soil of Western Ghats. They were tested to produce
enzymes such as cellulase, amylase, gelatinase, L-Asparginase, Pectinase, lecithinase, lipase,
lysine decarboxylase, oxidase, catalase, caseinase. The specific substrates were given as the
sole carbon source in respective medium.
The utilization of substrates by the Actinomycetes isolates was tested using a simple
sensitive agar plate with specific media. The positive result was shown by the presence of
zones and the specific color produced by the colonies of isolates. It was found that
Actinomycetes utilized almost all of the substrates. Maximum results were found in the
utilization of cellulose, starch, casein, lipids, and gelatin. More than half of the isolates were
able to utilize lysine, egg yolk and showed positive result. Very few isolates produced
specific enzymes to utilize L-aspargine and pectin.

Among isolates, V4 produced 10 enzymes except for L-asparginase. Isolates V1, V2, V3, V5,
V7, V11, V21; V22 produced 7-8 enzymes. These isolates can be used as a source of
multiple enzymes. The results prove that Actinomycetes isolates were diverse. They are a
source of industrially important enzymes for biodegradation of plant material, clearing of
oil spills, treatment of cancer, clarification of juices etc.
OP-LS-17
ANTI-CANCER ACTIVITY OF METHANOL EXTRACTS OF THE LATEX OF Michelia
champaca AND Calotropis gigantea using HUMAN BREAST CANCER CELL LINE ZR-75-
1 AND HUMAN LEUKEMIA CELL LINE HL60
Mehul Patel2, Vinit Rambhia1, Savan Savaliya2, Sam Balu1.

1 Biotechnology Finishing School, The Oxford College of Science, Bangalore 560102.
2 Department of Biotechnology, The Oxford College of Science, Bangalore 560102.
Corresponding Author: vrambhia@gmail.com
ABSTRACT
Cancer is the uncontrolled growth of abnormal cells in the body. Leukemia is a type of fluid
cancer while Breast cancer is one of the most commonly found solid tumour. Even though
pharmacological industries have produced a number of new drugs in the last few decades
against cancer, the requirement of a high potency compound still exists. Latex is a complex
emulsion consisting of proteins, alkaloids, sugars, oils, tannins, resins and gums. It serves
mainly as defense mechanism in plants. Anticancer activities of various extracts of the
medicinal plants have been widely demonstrated and are mainly associated with the
presence of different bioactive polysaccharides and tri-terpenoids. In the current study, we
have evaluated and compared in vitro the antitumor effects of methanol extract of the latex
of local plants against Human Breast Cancer Cell Line Zr-75-1 and Human Leukemia Cell
Line HL60. Experiments were carried out using various concentration of the compound
ranging from 10µg/ml to 80µg/ml. LC50, TGI and GI50 values were determined from the
observations and statistical calculations. SRB assay was employed to detect the cell growth
after the effect of the extracts. Adriamycin (Doxorubicin) was used as the positive control.

Results showed significant GI50 value below 10µg/ml for both the plants in comparison to
Adriamycin.
Keywords: Breast Cancer, leukemia, Calotropis gigantea, Michelia champaca, GI50,

Adriamycin.
OP-LS-18
PROPHAGE MEDIATED SERO-DIVERGENCE IN SALMONELLA SPECIES
Nancy Khess, Priyanka Bokade, Sangita Thounaojam, Sridhar J*, Sekar K V, Bharathi
S
Department of Microbiology, The Oxford College of Science, Bangalore, Karnataka.
*Faculty, NRCBS, Madurai Kamaraj University, Madurai, Tamilnadu.
Corresponding mail ID: nnckhess@gmail.com
ABSTRACT
Till date 45 Salmonella genomes have been sequenced completely. The objective of our
study is to analyse the prophage region, its annotated features, their role in virulence and
host specificity in all these genomes. Over extensive studies these Prophages were found in
mediating serovar based divergence in Salmonella species. After computational analysis of
the Prophages, our study revealed that prophage regions are present along with Salmonella
genome which were found to encode structural proteins as well as other proteins which
contributes to Salmonella pathogenicity. Through computational analysis 52 non-
redundant prophages like regions, among them some prophage elements like Gifsy_2,
Entero_2 etc were found at multiple locus within Salmonella genome. Out of fifty two, 20
were serovar specific and 22 were found to be strain specific Prophages. 5 Prophages like
entero_ES18, Salmon_1, Yersin_413C, Salmon_SPN3UB and Stx2_c_1717 were found to
encode host specific proteins. Genomic constructions in Salmonella serovars and their
distinct pathogenic features make them excellent models for studies of Salmonella host
adaptation and pathogenic divergence.The Prophages which were not revealed in the
earlier studies examples Phage_Escher_HK639, PHAGE_Yersin_413C and
PHAGE_Pseudo_F116 were observed during our study. These are the Phages derived from
different bacterial species and upon infection to salmonellaleads to divergence in its
serovars.S. enterica split from S. bongori as S. enterica encodes two complete type III

secretion systems encoded by SPI-1 (T3SS-1) and SPI-2 (T3SS- 2) whereas S. bongorilacks
SPI-2, which is required for optimal replication within macrophages Several characteristics
of S. bongori suggest that this species may, in evolutionary terms, lie somewhere between
E. coli and S. enterica. Similarly 2 unique prophages, entero_ES18 and ST64T of Salmonella
strains can sometimes leads to sero-divergence. On Blast analysis we found that they are
homologous to prophage entero_P22.
OP-LS-19
A FIELD STUDY ON COMPLEX INHERITANCE PATTERN OF DIABETES MELLITUS
Mrs Pavana Kamath, Athulya Mohan, Gayatri R.Iyer, Manasa Mahadevprasad, Chitra
Nambidi, Naveen V, Nijitha K, Aishwarya Panicker, Bushnin Patel, Shruthi R.
Department of Genetics, The Oxford of College of Science. HSR Layout, Bangalore 560 102
ABSTRACT
Urban and stressful standard of living can make us susceptible to a number of lifestyle
related disorders. India has more diabetics than any other country in the world, according
to the International Diabetes Foundation. The disease affects more than 62 million Indians,
which is more than 7.1% of India's Adult Population. An estimate shows that nearly 1
million Indians die due to Diabetes every year. The average age on onset is 42.5 years. The
high incidence is attributed to a combination of genetic susceptibility plus adoption of a
high-calorie, low-activity lifestyle by India's growing middle class. The Part 1 study
conducted in the year 2013 revealed that incidence of diseases like diabetes mellitus,
cardiovascular disorders and arthritis is more prominent in urban population than in rural
masses. A Part 2 study was conducted in the year 2014 to highlight on the link between the
genetic and the lifestyle component in these diseases. Aspects such as stress levels,
alcoholism and fitness were taken into consideration. The only chosen subjects were cases
with familial diabetic history and the additional health complications they suffer were
noted. It was seen that the genetic predisposition with modern lifestyle reduces the age of
onset of the diseases in subsequent generations – a phenomenon termed as Genetic
Anticipation.
Keywords – Diabetes, Genetic susceptibility, Lifestyle disorders.

OP-LS-20
COMPARATIVE MORPHOMETRIC ANALYSIS OF DIFFERENT SPECIES OF TERMITES

(ISOPTERA) FROM BANGALORE
Pranesh M. K1 and Harini B. P2
1Centre for Applied Genetics
2Department of Zoology, Bangalore University, Bangalore-560 056, Karnataka, India
ABSTRACT
Morphological study of termites is the most neglected criteria for assessing variation. In
this study 33 samples were collected from different localities of Bangalore which revealed
five widely distributed species Odontotermes ceylonicus (Wasmann), O. horni (Wasmann), O.
obesus (Rambur), O. redemanni (Wasmann) and Trinervitermes biformis (Wasmann). The
morphological data was subjected to different modes of statistical analysis. Manhattan
distance was constructed by coding mean values of different traits among the studied
populations. The Manhatten distance was compared with the dendrogram obtained by
coding Tukey-Kramer test (post hoc test) results from McQuitty distance. According to
Manhattan distance the similarity between the populations of the species was as expected.
In this analysis O. redemanni and O. obesus shows 100% similarity because the traits by
which the species are distinguished are not considered. The reason behind this is that not
all the species in this study contains mandible and left madibular tooth. The Tukey-Kramer
analysis O. redemanni and O. obesus show 50.31% of similarity which is an actual indication
of correlation coefficient. The correlation coefficient values were significant enough even
though the traits distinguishing the species were not considered. Due this reason for the
present study involving post-hoc test followed by correlation coefficient distance method
served more accurate results than Manhattan distance using the coded mean values.

OP-LS-21
STUDIES ON LOW COST MEDIUM IN PRODUCTION OF Chlorella Species USING COW
URINE FOR THE PRODUCTION OF BIO-DIESEL
Prasad .M.P
Sangenomics Research Lab, K.R.Colony, Domlur Layout, Bangalore 560071, India.
* Corresponding Author: E Mail- drprasadmp@gmail.com; Ph.: 9844357929
ABSTRACT
Microalgae are seen as an alternative and renewable feedstock for bio-diesel production.
The present work aims to study the growth of unicellular green algae Chlorella (C. vulgaris,
C. sorokiniana, C. heliozoae, C. lobophora and Chlorella pyrenoidosa) comparing Fogg’s
medium and cow’s urine as a low cost medium. Maximum biomass production was
achieved using 7.5% cow urine as the growth enhanced more than double in comparison to
normal Fogg's medium. The pH of both the medium was found to be between 7-8 which is
found to be favorable for Chlorella growth. Various parameters such as lipid, protein and
carbohydrate content was determined quantitatively and compared with normal grown
cells. The lipid content was found to be 34.7 % when 10 % of cow urine was used and
22.7% for 7.5% of cow urine used, which is found to be much higher than the Chlorella sp.
grown under normal Fogg’s media (7%). The protein content was also enhanced when cow
urine was used to grow the algal cells (50.17%) in comparison to normal grown cells
(38.24%). Carbohydrate content was reduced approximately to half in cow urine medium.
The fatty acids produced were also shown to differ in its composition when characterized
by GC-MS. The study confirms the use of Cow’s waste (cow urine) that is a rich source of
carbon, nitrogen, phosphorus and other micronutrients as a favorable medium for
cultivation of microalgae and bio-diesel production.
Keywords: C. vulgaris, C. sorokiniana, C. heliozoae, C. lobophora and Chlorella pyrenoidosa,

bio-diesel, cow waste, lipid content,

OP-LS-22
ANTIQUORUM SENSING ACTIVITY OF QUERCETIN AGAINST MULTIDRUG RESISTANT
Pseudomonas aeruginosa
Prity Lata., Joy Hoskeri H* and Mamatha J.*

Bangalore 560102, Karnataka, India.
E-mail: preetylatabiotech@gmail.com, joybioinfo@gmail.com
*Corresponding author: mamathaj.raj@gmail.com
ABSTRACT
Pseudomonas aeruginosa is a gram negative bacterium whose preferred mechanism of
metabolism is respiration. Thus it uses animals as host and its versatility enables it to infect
damaged tissues or even human with reduced immunity. P. aeruginosa are much more
complicated and can be life-threatening. Hence there is an immediate need for an efficient
drug or agent that can act as a magic bullet for the treatment of MDR P. aeruginosa
infections. Quercetin is a flavonoid it is found in fruits, vegetables, leaves and grains. So far
there are no reports available on antiquorum sensing activity of Quercetin. Hence in the
present investigation, we made an efficient attempt in evaluating the antiquorum sensing
property of quercetin against MDR P. aeruginosa in a dose dependent manner. Antiquorum
sensing activity of quercetin was studied by minimum inhibition concentration (MIC)
assay, swarming motility assay, flask incubation assay, and agar well diffusion assay. MIC
assay revealed that at160 µg/mL showed singnificant activitiy against MDR P. aeruginosa.
Agar well diffusion method also supported the results of MIC assay by showing 16 mm zone
of inhibition at same concentration. Swarming motility assay and flask incubation assay
where in corroboration with MIC assay and agar well diffusion assay indicating that
quercetin posseses potential antiquorum sensing activity against MDR P. aeruginosa. This
investigation reveals that quercetin is a strong antiquorum sensing agent against P.
aeruginaosa. Future investigation needs to be carried out to evaluate this property using in
vivo models.
Key Words: Pseudomonas aeruginosa, Quercetin, Multiple drugs Resistance (MDR),

Minimum inhibition concentration (MIC) and Antiquorum sensing.

OP-LS-23
ISOLATION OF OIL DEGRADING MICROORGANISMS FROM SOIL SAMPLE COLLECTED

FROM OIL SPILLED AREAS OF GARAGES.
Raji Sukumar,Gayatri R.Iyer,Athulya Mohan,NijithaK.,

Naveen V. and Bushnin Patel.
Department of Genetics,The Oxford college of Science,H.S.R. Layout, Bangalore
ABSTRACT
Oil spills have been recognized as one of the most serious current problem particularly in
industrialized and developing countries. Inevitable spillages, which occur during routine
operations of oil products, refining or as a consequence of acute accidents, lead
hydrocarbons to reach the water table before becoming immobilized in the soil. This study,
thus was aimed to assess the oil degrading potential of selected bacterial strain under in
vitro conditions.The present study is carried out by isolation of oil degrading microbes
from oil contaminated soil from the garages of Bangalore and identification of the
enzymes needed for degradation and the isolation of the degradative plasmids from the
micro organisms. Preliminary study was undertaken to determine the optimal conditions
for the biodegradation of a crude oil. Among 28 oil-degrading bacterial cultures isolated
from oil-contaminated soil samples, Bacillus sp, Pseudomonas sp were selected for the
study based on the efficiency of crude oil utilization. Along with the selected individual
strains, a mixed bacterial consortium prepared using the above strains were also used for
degradation studies. The culture medium included essential nutrients with prime carbon
source as the diesel oil.The mixed bacterial consortium showed more growth and
degradation than did individual strains. Degradation activity was determined using DCPIP
colorimetric analysis. 2,6-dichlorophenolindophenol is a chemical compound used as a
redox dye. Oxidized DCPIP is blue in colour showing maximum absorption at 600nm. At
1% crude oil concentration, the mixed bacterial consortium degraded a maximum of 77%
of the crude oil. Temperature of 35C and pH 7 were found to be optimum for maximum
activity.
Keywords: Bacteria ; oil Degradation; Hydrocarbons; DCPIP

OP-LS-24
HISOTPATHOLOGICAL STUDIES ON HYPOPHYSIS AND OVARY OF FRESH WATER FISH

Glossogobius giuris (HAMILTON)
S.Y. Narayanaswamy and M. Ramachandra Mohan

Department of Zoology, Dayananda Sagar College of Biological Sciences,
Kumaraswamy layout Bangalore: 560 078
Department of Zoology, Bangalore University, Bangalore: 560 056
ABSTRACT
Histopathology of the Hypophysis and Ovary was observed during preparatory phase, the
fish G. giuris on treatment with sublethal concentrations of Malathion ( 0.05 , 0.25 and 0.5
ppm) for 24, 48, 72 and 96 hrs intervals, histology represents a useful tool to assess the
degree of pollution, particularly for sub-lethal and chronic effects. However, a standardized
method for the description and assessment of histological changes, mainly for use in
freshwater fish, is still lacking. In this paper, the present authors propose a standardized
tool for the assessment of histological findings which can be applied to different
organs. The results of the present study showed morphological changes in the cell
structure. Investigation on Hypophysis and Ovary cells showed degranulation and
hypertrophy of cells and nuclei after the fishes were exposed for 24 hrs Malathion
treatment, these changes further intensified with 0.25 ppm treatment through the
appearance of extensive intercellular spaces. In higher concentration of Malathion
treatment with 0.5 ppm for 24 hrs, the cells showed various stages of degranulation which
were distributed sparsely. A reduction in the number and diameter of the Hypophysis and
Ovary cells indicates possible reduction in release of hormones. A higher concentration
brings about rapid changes of Hypophysis and Ovary which indicating a possible reduction
in the synthesis and release of hormones.
Keywords: Malathion , G.giuris, Ovary and Hypophysis

OP-LS-25
PRODUCTION OF STREPTOMYCIN FROM Streptomyces griseus BY SOLID STATE
FERMENTATION & ITS PRODUCTION ENHANCEMENT BY MUTATION & ANALYSIS BY
HPLC
Sudipa Bhadra., Amrutha C.H., and Mamatha J*.

Bangalore 560102, Karnataka, India.
*Corresponding author: mamathaj.raj@gmail.com
ABSTRACT
The actinomycete strain Streptomyces griseus isolated from soil is used for the production
of streptomycin, a potent antibiotic drug which is of great commercial importance. The
strain was identified by morphological features and several biochemical tests. Further they
were grown as a pure culture in starch casein agar media. Mutation was done both by
physical and chemical methods and it was found that the antimicrobial activity was
increased by 10% for the mutated strain kept under UV light for 5minutes. Production was
achieved by solid state fermentation using mousambi, pineapple and sugarcane waste as
the substrate which are cheaply available. Purification was done by filtration with charcoal
and acidified methanol followed by evaporation to produce antibiotic in powder form. High
purity was obtained during analysis by High Pressure Liquid Chromatography (HPLC) and
it is shown that the production increased by 24% with mousambi and 30% increase with
pineapple while there is a 46.77% increase in production with sugarcane using the
mutated sample. Hence it was concluded that mutation with UV light for 5mins and solid
state fermentation using sugarcane as substrate yielded the best results. This paper also
reviews the possible methods of increasing the production efficiency and cost effectiveness
of streptomycin at the industrial scale.
Key Words: Streptomyces griseus, Mutation, High Pressure Liquid Chromatography

(HPLC), Solid State Fermentation, Substrate.

OP-LS-26
IN VITRO QUALITATIVE AND QUANTITATIVE PHYTOCHEMICAL STUDIES OF

JASMINE SPECIES
Sushant Shekhar1*, Prasad M.P2.

1Department of Microbiology, Tumkur University, Tumkur, Karnataka
2Sangenomics Research Labs, Domlur, Bangalore, Karnataka
*Corresponding author. E-mail: shekharsushant21@gmail.com

Ph. No.: +91-9844796120
ABSTRACT
Traditional medical system has great value and also many medicinal plants have
been identified from indigenous pharmacopoeias, because of which plants are still
making imperative contribution to healthcare regardless of modern medicines which
has many advances. The medicinal values of plants lie in their phytochemicals, which
produce definite physiological actions on the human body. Phytochemicals are
compounds present in plants that are used as food and medicine to protect against
illness and to maintain human health. In the present investigation phytochemical
analysis was carried out from eight Jasmine species (Jasminum grandiflorum, J.
sambac, J. humile, J. sambac wild, J. arborescens, J. angustifolium, J. auriculatum, J.
officinale, J sambac L) with a view to access the therapeutic values and safety of the
plant in ethno medicine. The qualitative phytochemical analysis of these plants
showed the presence of saponin, terpenoids, glycosides and proteins in all the
Jasmine species. These Jasmine species showed complete absence of secondary
metabolites such as Phytosterols, tannins and lignin. The percentage of alkaloid was
93.3%± as recorder in J. angustifolium. The flavonoid content in J. sambac wild and J.
sambac cultivar variety was estimated at 55%±. Saponin was recorder at 18%± in J.
sambac wild variety.
Keywords: Quantitative phytochemical analysis, Qualitative phytochemical analysis,

secondary metabolites, Jasmine species, medicinal plants.

POSTER PRESENTATION

PP-LS-01
DIVERSITY OF FUNGI ON SOME SELECTED SPICES FROM BANGALORE RURAL
DISTRICT
B.K.Chikkaswamy Rabin and Chandra paramanik
5TH Main, Chamarajpet, Bangalore-560018
Sigma BioScience Research Center, 2 nd stasge, Indiranagar, Bangalore-38
ABSTRACT
In the present investigation an effort has been made to study fungal contamination of five
selected spices from Bangalore and the study resulted in the isolation of 20 fungal species
belongs to 42 genera out of which 10 species belongs to 5 genera of Zygomycetes, 2 species
belongs to 3 genera of Ascomycetes and 80 species belongs to 32 genera of Mitosporic
fungi. In Zygomycetes genera like Mucor, Absidia Rhizopus, and Syncephalastrum were
common. Genus Chaetomium in Ascomycets and in Mitosporic fungi genera like
Cladosporium, Curvularia, Aspergillus, Alternaria, Penicillium and Fusarium were most
predominant genera. Among the five selected spices Cumin samples shows highest number
of fungal species isolates than Coriander, Pepper, Cardamom and Indian Cassia samples
respectively. Spices are important agriculture commodities, because of their taste and
aroma they are widely used in the food preparations. Fungi are very predominant
contaminants of spices; they deteriorate the quality and taste of spice and it is one of the
major problems in spice trade. Fungal contamination of spices takes place at various levels
that is from field to fork level.
The highest percentage of occurrence was recorded in the genus Aspergillus (20.434%)
and lowest percentage of occurrence was recorded in Rhynchophoma (0.138%) and in
other genera, percentage of occurrence ranges between (0.126% to 24.23%).
Key words: Contamination and Percentage of occurrence. Diversity.

PP-LS-02
LEAF LITTER MYCOFLORA OF SOME MEDICINAL PLANTS FROM BANGALORE RURAL
DISTRICT
B.K.Chikkaswamy, Rabin Chandra paramanik and Prasad M.P
5th Main, Chamarajpet, Bangalore-560018
SigmaBioScience Research center, 2nd stasge, Indiranagar, Bangalore-38
ABSTRACT
The fertility of leaf litter depends upon its chemical components and quantitative nature of
microorganisms which inhabiting it. Among the microorganisms fungi are one of the
dominant groups. Leaf litter has been described as "poor man's rain forest" litter is the
material lying on the soil surface composed of plants and their shed organs like leaves,
twigs, etc which plays a major role in determining the structure and function of the
ecosystem and is a rich habitat for growth of microorganisms. In the present work a
detailed comprehensive survey of leaf litter fungi of medicinal plants viz Tinospora. and
Gymnema sylvestre . was undertaken and the study reveals the isolation of 120 species of
fungi which belongs to 54 genera out of which 22 species belongs to 7 genera of
Zygomycetes, 5 species belongs to 5 genera of Ascomycetes and 965 species belongs to 42
genera of Mitosporic fungi, no Basiodiomycetes were encountered. .Analysis of percentage
frequency reveals among Zygomycetes genera like Rhizopus, Cunninghamella were
dominant; in Ascomycetes Genera like Emerciella and Wentiomyces were dominant and in
Mitosporic fungi genera like Apergillus, Cladosporium, Fusarium were predominant. The
highest percentage of frequency of mycoflora was recorded in Gymnema sylvestre R. Br.
Than Tinospora .
Key words: Medicinal Plants and Percentage Frequency Leaf Litter, Mycoflora.

PP-LS-03
MOLECULAR DIVERSITY OF MUSHROOMS IN AND AROUND BANGALORE RURAL
DISTRICT, KARNATAKA.
B.K.Chikkaswamy Rabin Chandra paramanik
5TH Main, Chamarajpet, Bangalore-560018
Sigma BioScience Research center, 2 nd stasge, Indiranagar, Bangalore-38
ABSTRACT
The present investigation is an attempt to give a broad picture of biodiversity of
mushrooms belonging to the class Basidiomycetes in Bangalore. The surveys were
conducted from June 2012 to November 2013 in 8 different places which included scrub
jungles and urban places in and around Bangalore. A total number of 85 species in 50
genera belonging to 19 families in 05 orders were recorded, 28 species were found to be
recorded for the "first time in India and a new variety of Psathyrella candolleana var. indica
has been proposed. Out of 85 species 12 species were found to be edible and 20 species
have medicinal value. Among the collected species Coprinus disseminates followed by
Coprinus fibrillosis and Schizophyllum commune were found to be abundant in their
occurrence. The Simpson and Sannon diversity biodiversity index was found to be 0.7 and
1.22respectively.
Key words: Mushrooms in Bangalore, Karnataka.
PP-LS-04
MICROBIAL QUALITY ANALYSIS OF LOCALLY AVAILABLE HERBAL PRODUCTS.
Chaithra .U, Kavitha .M .T, Kavya .M, Sekar. K. V, *Asha. M . K and Bharathi . S
PG Department of Microbiology, The Oxford College Of Science,Bangalore.
*Department of Microbiology, Natural Remedies Pvt Limited,Bangalore.
Corresponding mail: kavitha.navya13@gmail.com.
ABSTRACT
Herbal products are preparation of various kinds of medicinal plants involving a dried
plant or any of its parts such as leaf, stem, root, flower and seed. Herbs are becoming more

popular throughout the world, as people strive to stay healthy in the face of chronic stress
and pollution and to treat illness with medicines that work in concert with the body’s own
defenses. The aim of the study was to evaluate the microbial contaminants of locally
available herbal products. Six herbal products were collected from market and microbial
quality control analysis was carried out by plating technique. All the six samples were
tested for the presence of Escherichia coli, Pseudomonas aeroginosa, Staphylococcus aureus
and Salmonella by using selective media. The results revealed that out of six samples two
samples were found to be contaminated with E.coli and one sample was found to be
contaminated with Salmonella. The total bacterial count of all the test herbal products
ranged from 1- 8*102 cfu/ml/g and the total fungal count of all the products were found to
be zero. As it has been noted in present days there is an increasing demand for Ayurvedic
medicines, but some are hesistant to take them because of the fear of contamination due to
microorganisms ,therefore such studies plays an important role in promotion of the herbal
products over the chemically prepared products which are known to have higher side
effects.
PP-LS-05
STUDIES ON AMYLASE PRODUCTION FROM Aspergillus Oryzae BY
SOLID-STATE FERMENTATION
Ankita Dey, Anindita Maji and Abdul Nayeem*,
HSR Layout, Bangalore.
*Coresponding author: nayeemqaadri@gmail.com
ABSTRACT
Amylases (α-amylase, β-amylase and glucoamylase) are among the most important
enzymes in present day biotechnology. Although amylases can be derived from several
sources, microbial enzymes generally meet industrial demands. These enzymes, especially
α-amylase have found wide applications in a number of industrial processes such as in the
food, fermentation, textile and paper industries. Industrially important enzymes including
amylases have traditionally been obtained from submerged fermentation (SmF) because of
ease of handling and greater control of environmental factors such as temperature and pH.

Solid state fermentation (SSF) constitutes an interesting alternative since the metabolites
so produced are concentrated and purification procedures are less costly.
Solid-state fermentation presents a series of advantages over submerged fermentations

and these have been widely discussed in the literature. The culture conditions are more
similar to natural habitat of filamentous fungi, so that they are able to grow and excrete
large quantities of biomaterials. Products concentration after extraction is usually higher
than those obtained by submerged fermentation and the quantity of liquid waste generated
during the fermentation process is lower. SSF has gained renewed interest from
researchers for enzymes production in view of its economic and engineering advantages
and is often employed to produce amylases. Filamentous fungi have a number of properties
which make them important both scientifically and industrially. They are also suitable
microorganisms for SSF, especially because their morphology allows them to colonize and
penetrate the solid substrate has been successfully used to express high levels of
heterologous proteins and industrial enzymes.
The present study was made to assess the effect of wheat bran and other environmental
parameters such as pH, temperature, culture age etc. on the production of amylases, in
particular, α-amylase. Aspergillus oryzae was collected from Dept. of Microbiology, The
Oxford College of Science, Bangalore and pure culture was maintained on MRBA medium.
Wheat bran was autoclaved and different ratios of minimal sat medium (1:1 and 1:5) were
mixed and 1 ml of A. oryzae spore suspension was inoculated into the flask
containing wheat bran and minimal salt medium. The production of α-amylase was
determined at different time intervals of 48h, 72h, 96h and 120h and different inoculums
size of 1 ml, 2 ml and 3 ml. The highest production of α-amylase was recorded at 120h with
1.0 ml inoculum. The effect of pH and temperature are being carried out.

PP-LS-06
EFFECT OF USE OF DIFFERENT LOW CALORIE INTENSE SWEETENERS AND OVERALL
QUALITY OF YOGHURT, BIOYOGHURT AND PROBIOTIC MISHTI DAHI.
Chethana.B, Monica Devi.V, Poornima.S, Sowmya.V, Bharathi.S, Pramod Taranth and
Natarajan.A
Department of Microbiology, The Oxford of College Of Science, B’lore-90
Corresponding mail: drpramodoxford@gmail.com
ABSTRACT
The aim of this investigation was to enhance the nutritive, therapeutic value and effect of
addition of synthetic sweetener on the overall quality of probiotic fermented milks and also
developing low calorie probiotic yoghurt fortified with the functional ingredients.In recent
years there has been a sea change in the public preferences for foods. As a result, a number
of foods containing probiotic have been introduced. A number of beneficial effects have
been listed caused by the lactic probiotic cultures delivered mostly through fermented
milks. These probiotic lactic organisms are termed as “Colonic foods”, as these upon
entering the colon, establish in the intestinal tract, and thereby provide the host with a
number of metabolic compounds which are involved in providing various health benefits.
Such probiotic organisms in the form of bio yoghurt consisting of these lactic acid bacteria
namely, S.thermophilus, B.bifidus and L.acidophilus were used in the preparation of
fermented milk, and used as a base for developing value added full and low calorie
probiotic Mishti dahi.The nutritive and therapeutic value of the bio yoghurt Mishti dahi was
thus developed to benefit the consumer further by fortifying it with functional ingredients
such as grape seed extract, green tea extract, premix etc. to help address current health
issues and in part health benefits.The outcome of this study was the development of a
beverage with low and full calorie probiotic Mishti dahi with enhanced therapeutic and
health benefits to the common man who consumes this beverage on a regular basis.The
findings of this investigation would benefit a large section of population by contributing
today to the health benefits of the common man. The proverb “Health is Wealth” aptly
ensures the importance of health in our day today life.

PP-LS-07
COMPARATIVE STUDY OF ANTI-OXIDANT AND ANTI-DIABETIC ACTIVITY OF Kigelia
pinnata
Dhriti V1., Chowdary P. V. V1., Rahul J1., Vishank G1., Shivaji B. Bole1*.
Dept of Biotechnology, Biotech Finishing School, The Oxford College of Science, 17 th B, HSR
layout, Bangalore.
*Shivaji.B. Associate Professor, Department of biotechnology,
The Oxford College Of Science, Bangalore, Karnataka-560102
Email:shivajibio@gmail.com, +91 9611922895, Fax: (080) 30266349
ABSTRACT
Medicinal plant plays an essential role in the management of various diseases. Kigelia
pinnata commonly known as the Sausage tree, it is a multipurpose medicinal plant with
many characteristics and considerable potentials. The plant has traditional uses which
consist of anticancer, antiulcer, anti-aging, antioxidant, and anti malarial, in the treatment
of genital infections, gynaecological disorders, renal ailments, fainting etc., this work
represents the recent profile of applications of Kigelia pinnata as examined by different
contemporary scientific researches. In this study, the in vitro anti-oxidant activity, anti-
diabetic activity and phytochemical evaluation of four different solvent extracts, from the
leaves of Kigelia pinnata were determined. Phytochemical screening of the extracts of the
leaves showed the presence of flavanoids, alkaloids, etc. Anti-oxidant activities of these
extracts were evaluated through DPPH assay, reducing power assay and phospho-
molybednum assay. The anti-diabetic activities of these extracts were determined by
amylase inhibition assay. The anti oxidant and anti-diabetic activity of extracts was found
to be promising camparble with standard. IC50 was calculated for both antioxidant and
antidiabetic activity.The results obtained showed that the leaves of Kigelia pinnata has
anti-oxidant and anti-diabetic properties, which helps in providing a basis for the
traditional use of medicinal plant.
Key words: Bignoniaceae, Kigelia pinnata, anticancer, anti-oxidant, anti-diabetic, DPPH.

PP-LS-08
BIOSORPTION OF ZINC BY HEAVY METAL RESISTANT FUNGI ISOLATED FROM
BELLANDUR LAKE
Ganesha R, Dhondup Namgyal, Kiran Kumar S, Atul Chandra and Abdul Nayeem*,
P.G. Department of Biotechnology, The Oxford College of Science, HSR Layout, Bangalore.
*Corresponding author: nayeemqaadri@gmail.com
The discharge of heavy metals into aquatic ecosystems has become a matter of concern in
India over the last few decades. These pollutants are introduced into the aquatic systems
significantly as a result of various industrial operations. The pollutants of concern include
lead, chromium, mercury, uranium, selenium, zinc, arsenic, cadmium, gold, silver, copper
and nickel. These toxic materials may be derived from mining operations, refining ores,
sludge disposal, fly ash from incinerators, the processing of radioactive materials, metal
plating, or the manufacture of electrical equipment, paints, alloys, batteries, pesticides or
preservatives. Heavy metals such as zinc, lead and chromium have a number of
applications in basic engineering works, paper and pulp industries, leather tanning,
organochemicals, petrochemicals fertilizers, etc. Major lead pollution is through
automobiles and battery manufacturers. For zinc and chromium the major application is in
fertilizer and leather tanning respectively.
Over the few decades, several methods have been devised for the treatment and
removal of heavy metals. Bioremediation consists of a group of applications, which involve
the detoxification of hazardous substances instead of transferring them from one medium
to another, by means of microbes and plants. This process is characterized as less
disruptive and can be often carried out on site, eliminating the need to transport the toxic
materials to treatment sites. The biosorption (sorption of metallic ions from solutions by
live or dried biomass) offers an alternative to the remediation of industrial effluents as well
as the recovery of metals contained in other media. Biosorbents are prepared from
naturally abundant and/or waste biomass. Due to the high uptake capacity and very cost-
effective source of the raw material, biosorption is a progression towards a perspective
method. The mechanism by which microorganisms take up metals is relatively unclear, but
it has been demonstrated that both living and non-living biomass may be utilized in

biosorptive processes, as they often exhibit a marked tolerance towards metals and other
adverse conditions.
In the present study, soil samples were collected from Bellandur Lake, serially
diluted and inoculated on Tryptone glucose extract agar plates with various concentrations
of zinc ranging from 100 to 1000 mg/l. The minimum inhibition concentrations (MICs)
revealed that four isolates were able to tolerate a high concentration of zinc (1000mg/l).
The isolates were maintained by weekly subculturing on Tryptone glucose extract agar
medium. All the isolates were inoculated to Tryptone glucose extract liquid medium and
incubated on a rotary shaker at 280 C for 72 hours. The flasks were taken out after 72 hours
of inoculation and were centrifuged at 10,000 rpm for 10 min at 4°C. After centrifugation
the supernatant (three times washed with NaOH 1N) were digested with HNO 3 67% and
H2O2 (30% v/v), and metal concentration was determined by atomic absorption
spectrometry.
The results showed that all the fungal isolates showed efficient Biosorption
property. The isolate 8, showed maximum Biosorption compared to other isolates in the
order 148>134>85>36 mg/l for the isolates Zn8>Zn4>Zn6>Zn1 respectively. The
characterization and identification of the fungi is in progress.
PP-LS-09
ALTERNATIVE METHOD OF EXTRACTION OF EUGENOL FROM SYZYGIUM
AROMATICUM AND ITS APPLICATIONS
Hima CS, Hitha CS, Bharathi S and *Walter Schrenk
Department of Microbiology, The Oxford College of Science, Bangalore
*Amrita – Agilent Research Lab, Amritapuri, Kerala
Corresponding E-mail: himacs.90@gmail.com
ABSTRACT
Eugenol is the main ingredient of the essential oil that can be isolated from the spice
Syzigium aromaticum (cloves). The clove is the bud of an East Indian ever green tree.
Phenolic phytochemicals are a broad class of nutraceuticals found in plants which have
been extensively researched by scientists for their health-promoting potential. One such a
compound which has been comprehensively used is eugenol (4-allyl-2-methoxyphenol),

which is the active component of Syzigium aromaticum. Aromatic plants like nutmeg, basil,
cinnamon and bay leaves also contain eugenol. Eugenol has a wide range of applications
like perfumeries, flavorings, essential oils and in medicine as a local antiseptic and
anesthetic. Increasing volumes of literature showed eugenol possesses antioxidant,
antimutagenic, antigenotoxic, anti-inflammatory and anticancer properties. In the present
study eugenol was extracted by steam distillation method. Analytical methods such as
HPLC and UV Spectrometry were used for further analysis of eugenol.
Keywords: Eugenol, Cloves, Anticancer and HPLC.
PP-LS-10
IN-VITRO AND IN-SITU STUDIES ON MICROBIAL DETOXICATION OF PLASTIC WASTE
FROM THE DUMPING SITES OF BANGALORE CITY
Sujan, Lakshmi C, Deepthi Arun Varier and Bharathi S
Corresponding E-mail ID – hithacs.90@gmail.com
ABSTRACT
Plastic waste is accumulating in the environment are causing an ever increasing ecological
threat. Plastic causes serious damage to the environment: both during its production and
disposal. Biodegradation of plastic waste using microbial strain could offer a solution to
this problem. Microorganisms have been a good resource for solution to this problem due
to their diverse metabolic capability, adaptability to different environment and possibility
of isolation using semi synthetic minimal media.
In the present study, potent microbial strains degrading plastics (LDPE and PVC) were
isolated using soil burial technique. To increase the chances of isolating such strains which
could have adapted to metabolize plastic constituting polymers, samples were collected
from Mavallipura dumpsite, Bangalore, which were rich in plastic waste. The end of the
study, 2 fungal species and 4 bacterial species were isolated on MSV medium supplemented
with emulsified plastic. The microbial species associated with the, PVC materials were
identified as Kingella, Actinomyces, Penicillium and LDPE materials were identified as
Alcaligens, Methylobacillus, Aspergillus flavus. The biodegradation of the respective
plastics was analyzed for after 2,3 and 4 months of incubation in soil. The degradation was
obtained as 2% weight loss for PVC sample and 6% weight loss for LDPE after 2 months,
10% weight loss for PVC and 15% weight loss for LDPE after 3 months, 21.3% weight loss
for PVC and 26.1% weight loss for LDPE after 4 months. Optimization methods were
employed to monitor different parameters like pH, temperature and concentration of
plastics for the increased biodegradation of the respective plastics. Optimum pH was found
to pH 8, temperature was found to be 37°C and plastic concentration was found to be
0.50% for both bacterial and fungal isolates identified. The soil sample analyzed for the
Micronutrients, Macronutrients & also heavy metals. The soil sample collected was alkaline
. The electrical conductivity was found to be ideal. Organic matter was deficient.
Phosphorous was also found deficient. Potash level was found ideal. Iron content was high
.Manganese, Zinc and Copper level also found high. Secondary Nutrient and heavy metal
also found low.
PP-LS-11
COMPARATIVE EVALUATION OF THREE MEDICINAL PLANTS FOR ANTIBACTERIAL
ACTIVITY AGAINST IMPORTANT SPECIES OF BACTERIA.
John Louis.A1, Catherine Albina.A2 , Kiran.B3
1PG
Department of Biochemistry, CMR Institute of Management Studies
(Autonomous) Bangalore -560043.
E.mail: johnlouis88@gmail.com., P.No. 8123482470.
2PG Department of Biochemistry, CMR Institute of Management Studies
(Autonomous) Bangalore -560043. E.mail: catz4alb@gmail.com., P.No. 7204286503.

3Head of the Department, PG Department of Biosciences, CMR Institute of
Management Studies (Autonomous) Bangalore -560043.

E.mail: bkiran2702@gmail.com., P.No. 9379267558.
ABSTRACT
in vitro evaluation of three medicinal plants viz., Ficus microcarpa L., Antidesma acidum
Retz., and Valeriana hardwickii Wall. against three bacterial species Escherichia coli,
Bacillus subtilis and Staphylococcus aureus tested at 10, 20, 30 40 and 50 microliter
concentration. F. microcarpa recorded a maximum inhibition of 34.0mm at 50 µl
concentration against S. aureus. 26.0 µl against B. subtilis at 50 µl concentration and
20.0mm against E. coli. V. hardwickii recorded a significant activity and showed 24.0mm,

20.0 and 12.00mm inhibition against B. subtilis , E. coli and S. aureus respectively tested at
50 µl concentration. Compared to synthetic antibiotic chloramphenicol tested at 25mg
concentration, significant activity was observed against all the test bacteria and recorded
18.0mm to 31.0mm inhibition.
Key words: Ficus microcarpa , Antidesma acidum , Valeriana hardwickii, antibacterial

activity, synthetic antibiotic.
PP-LS-12
HYDROXYL RADICAL SCAVENGING AND 1, 1-DIPHENYL-2-PICRYLHYDRAZYL (DPPH)
RADICAL SCAVENGING ACTIVITY OF Psoralea corylifolia L .
Kiran.B1 Lalitha.V2and Raveesha.K.A3
Head of the Department, PG department of Biosciences, CMR Institute of Management

Studies (Autonomous) , Bangalore -560043. E.mail: bkiran2702@gmail.com,
P.No. 9379267558.
Assistant Professor, Department of Studies in Botany and Microbiology, Maharanis Science
College for Women, Bangalore-560001. E.mail: lalithavkiran@gmail.com , P.No.
9844142520.
Professor, Department of Studies in Botany, Manasagangotri, University of Mysore, Mysore
-570 006. E.mail: karaveesha@gmail.com , P.No. 9845481329.
ABSTRACT
Among the various extracts tested, AWEP (Alcohol Water (1:1) Extract of P. corylifolia)
showed highest hydroxyl radicle scavenging activity of 87.0% at 20µg. Compared to
standard BHA and α- tocopherol at 80µg showed 87.46% and 88.13% respectively. AWEP
is followed by AEP(Alcohol Extract of P. corylifolia seeds) (70.0% at 20µg), WEP(Water
Extract of P. corylifolia) (60.0% at 20µg), HEP(Hexane Extract of P. corylifolia seeds)
(55.0% at 20µg) and CEP(Chloroform Extract of P. corylifolia seeds) (52.0% at 20µg).
Hence AWEP could be an effective nutraceutical antioxidant and hydroxyl radicle
scavenger at very low concentration. Compared to other extracts of P. corylifolia(Seed) and
standard antioxidants. Among the different extract of P. corylifolia (Seed), AWEP showed
maximum and powerful DPPH radicle scavenging activity by 89.0% at 25µg. This is
followed by AEP (63.3% at 100µg), HEP (50.2% at 100µg), WEP (50.0% at 100µg) and CEP

(49.0% at 100µg) respectively. When compared with nutraceutical antioxidants α-

tocopherol (76.3% at 85.0µg) where as non-nutraceutica antioxidants BHA showed 82.0%
at 100µg).
Key words: Hydroxyl radical scavenging, 1, 1-diphenyl-2-picrylhydrazyl (DPPH), radical
scavenging , Psoralea corylifolia L ., Antioxidant activity.
PP-LS-13
PRODUCTION OF BIODIESEL FROM MICROALGAE, EDIBLE AND NON-EDIBLE OILS
Lakshmi.C, Lavanya.R, Swetha Rani.S.R, Sujan R.A, Bharathi.S and Harini Kumar.K.M
Department of Microbiology, The Oxford College of Science, Bangalore-560102
Corresponding e-mail id- bartiravi@gmail.com
ABSTRACT
Biodiesel is an alternative biofuel which is produced by a mono-alcoholic
transesteriﬁcation process, in which triglycerides reacts with a mono-alcohol with the
catalysis of alkali, acids, or enzymes. As known, biofuel can be produced from various
plants. But their increased use for the production of biofuel may create an ecological
imbalance and also affects the food security. This limits the use of plants and increases the
use of microorganisms such as Microalgae in the biofuel production. Micro-algae are the
fastest growing photosynthesizing unicellular organisms and can complete an entire
growing cycle every few days. Some algae species have high Oil content and can produce up
to 15,000 gallons of oil per acre per year under optimum conditions. Photosynthesis is the
first step in the conversion of light to chemical energy and ultimately responsible for
supporting all biofuel synthetic processes, converting solar energy into biomass, carbon
storage products and hydrogen. Bio-Lipid oil can also be used to produce one of the lowest
cost and most environmentally friendly fuels. Bio-diesel made from bio-lipid oil, from
micro-algae, burns cleaner, produces less CO 2 than petroleum based diesel fuels, and it's
renewable and sustainable. The algae used to produce the bio-lipid oil actually absorb CO2
from the atmosphere, thereby reducing dangerous, greenhouse gases that cause global
warming.
The aim of our project was to extract biodiesel from microalgae collected from the mass
cultivation unit of Bangalore (GKVK) and from edible & non edible oils collected from oil
extraction unit (GKVK) and few oil samples from provision stores. The isolates of
microalgae were obtained by culturing the water samples on BG11 media & SP media.
Microscopic observation was carried out to confirm the growth of microalgae. Lipid
estimation was carried out to determine the highest lipid content in the samples. BG11
media (A1) sample proved to have highest lipid content of 0.25083mg/ml compared to
other samples. Micro algal biomass was used for bio diesel extraction. Edible & Non edible
oils were also used for the extraction of biodiesel. The composition of the biodiesel was
analyzed by performing several confirmatory tests for all the oils.
PP-LS-14
EFFECT OF CULTURE FILTRATE OF Trichoderma species AGAINST IMPORTANT SEED
BORNE FUNGAL PATHOGENS OF PADDY
Lalitha.V1, Kiran.B2 and Raveesha.K.A3
Assistant Professor, Department of Studies in Botany and Microbiology, Maharanis Science
College for Women, Bangalore-560001, E.mail: lalithavkiran@gmail.com., P.No.
9844142520.
Head of the Department, PG department of Biosciences, CMR Institute of Management
Studies (Autonomous) Bangalore -560043. E.mail: bkiran2702@gmail.com.,
P.No. 9379267558.
Professor, Department of Studies in Botany, Manasagangotri, University of Mysore, Mysore
-570 006. E.mail: karaveesha@gmail.com , P.No. 9845481329.
ABSTRACT
Culture filtrate of all the Trichoderma sps tested showed significant activity against test
pathogens of paddy. The percent inhibition of the test pathogens in T. viride treatment
varied between 13.7%(Fusarium oxysporum) to 82.0% (Pyricularia oryzae), In case of T.
harzianum, it varied between 13.0% (F. oxysporum) to 82.0% (Curvularia lunata). In case
of T. virens, the inhibition percentage varied between 24.44% (F. solani) to 87.36%
(Drechslera halodes). Similarly in T. koningii treatment, the percent inhibition ranged
between 69.0% (T. padwickii) to 100.0% inhibition (Pyricularia oryzae and F. oxysporum).
Among the treatments, total inhibition was observed only in case of T. koningii against
P. oryzae and F. oxysporum and none of the other treatments recorded total inhibition.
Among the culture filtrates of Trichoderma sps tested it is observed that culture filtrates of

T. koningii recorded significantly higher percentage of inhibition against all the test
pathogens followed by T. viride, T. virens and T. harzianum. More than 80% inhibition of
the test pathogens was observed in case of T. koningii treatment except T. padwickii and D.
tetramera.
Key words: Biocontrol, antimicrobial activity, Paddy, seed borne pathogens.
PP-LS-15
SOLID WASTE TREATMENT AND ITS CONVERSION TO AN ECO-FRIENDLY PRODUCT
BY EFFECTIVE MICROORGANISM (EM) TECHNOLOGY
Madhumita Ghosh Dastidar, Shankar BS
Department of Microbiology, Vijaya College, R.V.Road, Bangalore
Email- madhumita.dastidar@gmail.com
ABSTRACT
In a modern city life we have to deal with enormous wastes that generate everyday through
industrial wastes and domestic wastes. The quantity of wastes generated due to
urbanization pose a major problem of proper and adequate treatment and has an adverse
effect on environment. Many scientific techniques have been discovered for proper waste
management followed by its disposal. But today it is necessary to use such a method which
can convert wastes to reusable products, thus aim to combat environmental pollution. The
kitchen waste is one of the typical household generated waste shows a major impact on
municipalities for the treatment. The waste management by Effective Microorganism (EM)
technology has been adapted by many countries.
The aim of this study is to find an easier solution to treat solid wastes by EM technology
and conversion of the wastes to eco friendly products. This study has a number of
applications including agriculture, livestock, gardening, landscaping, composting,
bioremediation etc. The wastes have been successfully converted by EM methods. Also
when analyzed the content of carbon, nitrogen and phosphorous of the wastes after EM
treatment, it has been observed that these elements are increased which can be utilized as
source biofertilizers.

PP-LS-16
A STUDY ON THE EFFECT OF SALON WASTES ON HUMAN HEALTH AND A NOVEL WAY
TO UTILIZE THE WASTE HAIRS
Madhumita Ghosh Dastidar, Pooja Y.V, Rashmi M, Shalini S.G, Varsha N
Department of Microbiology, Vijaya College,R.V.Road
Email - madhumita.dastidar@gmail.com
ABSTRACT
Salon and spa are mushrooming everywhere and doing brisk business too. Salon waste
products if disposed untreated causes adverse effects on health and surrounding
environment which enhances the growth of various pathogens especially related to skin.
The major health issues like skin rashes, eye irritations, scalp itching are caused by salon
wastes like hair clippings, various chemicals, dyes and also used cotton, tissue paper etc.
The aim of the experiments to analyze the indoor micro flora and also to find applications
of salon wastes.
We collected various samples like hair, nail scalp and skin swabs of salon worker and
conducted various microbiological tests to analyze the internal environment of a salon.
The industrial growth has accelerated the generation of various oily wastes especially from
petrochemical, metallurgical industries and transportation and domestic sewage wastes.
These wastes are major pollutant for aquatic environment. Industrial waste contain 75%
free oil, 20% emulsified oil and 5% soluble oil. Separation of oil from surface of a large
body of water is necessary otherwise aquatic life will be under threat.
Everyday thousands of tons of hair are generated as wastes from salon which are thrown to
landfills with no direct benefit. Moreover hair is a slow degradable product. Our aim is to
find an effective means of utilizing the salon generated wastes hair to clear the oil spills.
Human hair can adsorb various potential hazardous oils like crude oil, motor oil which
upon spillage causes environmental pollution. We have tested the human hair oil
adsorption capability by testing with various types of hair. The proportion of oil adsorption
is quantified by considering various properties of oil adsorption. Finally we have converted
the oil laden hair to an eco friendly bio-compost.

PP-LS-17
ANTIBACTERIAL STUDIES OF Caesalpinia bonducella SEED EXTRACTS
Mousami Gosh, Wepfule Kapfo and Abdul Nayeem
P.G. Department of Biotechnology, The Oxford College of Science, HSR Layout, Bangalore.
ABSTRACT
The toxicity to humans and other animals from antibiotics is generally considered to be
low. However, prolonged use of certain antibiotics can decrease the number of gut flora,
which can have a negative impact on health. The inevitable consequence of widespread and
injudicious use of antibiotics has been the emergence of antibiotic-resistant pathogens,
resulting in a serious threat to global public health. The resistance problem demands that a
renewed effort be made to seek antibacterial agents effective against pathogenic bacteria
resistant to current antibiotics. Possible strategies towards this objective include the
increased sampling from diverse environments and identification of bioactive compounds
produced by plants and other sources.
Caesalpinia bonducella, commonly known as Gray Nicker, is a species of flowering
plant of family Caesalpiniaceae, that has a pantropical distribution. C. bonducella Fruit is
also known as a Fever Nut, due to its antipyretic properties. The seeds contain an alkaloid
caesalpinine, bitter principles such as bonducin, saponins and mixed oils. These
compounds render the herb its therapeutic properties. The plant treats skin diseases,
ulcers and leprosy, gastrointestinal problems like, dysentery and diarrhea.
C. bonducella seeds were collected from Junagadh forest, Gir area (Gujarat). C. bonducella
seeds were crushed in a mixer & dry powder formed. The powder was subjected to
soxhalet extraction. All the extracts at various concentrations were tested for antibacterial
studies on various strain of microorganisms like Pseudomonas aeruginosa, Shigella
dysentery, Proteus mirabilis, Staphylococcus aureus, Enterococcus faecalis. chloroform
extract have showed good antibacterial activity against Shigella dysentery, Proteus mirabilis,
pseudomonas aeruginosa, Enterococcus faecalis. Methanol extract have showed good
antibacterial activity against, Proteus mirabilis, pseudomonas aeruginosa. Petroleum ether
extract have showed good antibacterial activity against Proteus mirabilis. Aqueous extract

have showed good antibacterial activity against Proteus mirabilis. There is no overcoming
of growth in the zone of inhibition with prolonged incubation.
PP-LS-18
ELISA- BEST ADVANCED HANDY DETECTION TEST OF PICKING THE SPECIFIC
PROGESTERONE METABOLITE IN FECAL SAMPLES OF WILD ASIAN ELEPHANTS!
S.B.Chethna
MSc. Biological Sciences, Bangalore University, Bangalore 560008
ABSTRACT
Asian elephants (Elephas maximus) are prominent “flagship species”, are listed under the
category of endangered species (EN-A2c, ver.3.2; IUCN Red list 2009) and there is need for
their conservation. This requires understanding demographic and reproductive dynamics
of species. Traditionally invasive blood sampling is carried out but this is restrictive for
large animals and hence non- invasive methods are ideal to assess the reproductive
cyclicity of elephants to detect the concentration of catabolised progesterone levels in
female elephants. The diet and the ecological parameters are vital for normal progesterone
synthesis. These are poly breeders and come into estrous about thrice a year due to longest
estrous cycle of about 16 weeks. And only during estrus are the chances of pregnancy,
which is directly proportional to progesterone levels. So assaying this technique by Enzyme
linked immune sorbent assay (ELISA) is the latest and handy method. This advanced
technique has ample advantages over Radio Imuno assay (RIA) and is highly specific and
sensitive shows 90% binding to pick up this progesterone metabolite. The purpose of this
study is to estimate the concentration of one of the progesterone metabolites i.e
allopregnanolone (5α-P-3OH) in different fresh fecal samples of the random population
subjected to a representative block of Asia. The results will help us out to predict the
reproductive status for the localized population of female elephants, thereby helping to
plan their breeding strategy.
Keywords: Flagship, Non-invasive and reproductive cyclicity.

PP-LS-19
EFFECT OF AQUEOUS AND SOLVENT EXTRACT OF Piper longum L. ROOT AGAINST
IMPORTANT SPECIES OF BACTERIA
Sakchi Bhushan1, Madhubala Shriram2 and Kiran.B3
1PG Department of Biotechnology, CMR Institute of Management Studies (Autonomous) ,
Bangalore -560043. E.mail: 4sakchi8bhushan@gmail.com P.No. 9535601823.
2PG Department of Biotechnology, CMR Institute of Management Studies (Autonomous) ,

Bangalore -560043. E.mail: shriram.madhu@gmail.com., P.No. 9738430337.
3Head of the Department, PG Department of Biosciences, CMR Institute of Management

Studies (Autonomous) , Bangalore -560043. E.mail: bkiran2702@gmail.com.,
P.No. 9379267558
ABSTRACT
Evaluation of antibacterial activity of roots of Piper longum L. belongs to family piperaceae
against four Gram negative human bacteria viz., Escherichia coli, Pseudomonas aeruginosa,
Salmonella typhi and Klebsiella pneumonia and two Gram positive bacteria viz., Bacillus
subtilis and Staphylococcus aureus in vitro condition. The aqueous extract was tested at 10,
20, 30, 40, 50, 60, 70, 80, 90 and 100 % concentration . Significant activity was observed in
70, 80, 90 and 100 % concentration and recorded 25.0mm to 36.0mm inhibition. Among
the different solvent extract viz., petroleum ether, chloroform, benzene, methanol and
ethanol tested at 10 to 100% concentration, maximum activity was observed in petroleum
ether extract and recorded 28.0mm to 36.0mm inhibition against test bacteria, followed
by methanol, chloroform and ethanol. The Minimum Inhibitory Concentration (MIC) was
also determined for all the species of bacteria. All the result was compared to synthetic
antibiotics Gentamicin (25mg), Tetracycline (25mg) and Streptomycin (25mg).
Key words: Piper longum L, antibacterial activity, synthetic antibiotic

PP-LS-20
EFFECT OF INTERACTION OF SOLUBLE STARCH AND STABILIZER ON THE GROWTH
BEHAVIOUR OF LACTIC ACID BACTERIA
Saurav kumar, prince kumar, Krishna kumar, Bharthi.S
Department of Microbiology, The Oxford College of science, Bangalore
ABSTRACT
Soluble starch is a high molecular- weight, water- soluble dextrin produced by the partial
acid hydrolysis of starch. Stabilizers are complex polysaccharide which are added to food
stuff like yogurt to enhance its texture, flavour. Lactic acid bacteria(LAB) are gram +ve, rod
shaped bacteria which are mainly present in milk and its product like curd, yogurt etc.
some of the species of LAB are considered to be Probiotic cultures. Soluble starch and
stabilizer mainly contribute to firmness, viscosity, water holding capacity and protein
stability in Yogurt. Soluble starch and Stabilizer were added to Yogurt with an aim to get
Yogurt product with firmness, viscosity, flavour having considerable health benefits in
condition like Antibiotic-associated diarrhoea(AAD), Pseudomembranous colitis etc.
The objective of the work was to observe the growth of lactic acid bacteria after
interaction of Soluble starch and Stabilizer. Different commercially available from of
soluble starch were used out of which Curia-2 was screened and 1% concentration of
Curia-2 was found to be the best and Sodium alginate was found to be the best out of
different stabilizers used such as Pectin, Locust Bean Gum(LBG.)Raw milk sample used for
Yogurt preparation contained 4% fat, 9.065% SNF, and 13.065% TS. Culture used for
Yogurt preparation was Lactobacillus bulgaricus and Streptococcus thermophilus.The
physicochemical characteristics of yogurt with interaction of soluble starch and stabilizer
differ significantly In comparison with control sample. Addition of soluble starch and
stabilizer enhances the consistency, firmness and adhesion of product. By the help of
growth curve it was concluded that interaction of soluble starch and stabilizer enhance the
product quality and increased growth rate of LAB.

PP-LS-21
BACTERICIDAL, FUNGICIDAL AND ANTHELMINTIC ACTIVITIES OF Alstonia scholaris
BARK EXTRACTS
Naveen Kumar H.N.,1Badarinath D.K.,1 Joy Hoskeri H.,1,2Tarveen Jandoo,1 Savaliya
Savan and Vedamurthy A.B.,3*
1Department of Biotechnology, The Oxford College of Science,
Bangalore – 560 102, Karnataka, India.

2Department of P.G. Studies and Research in Biotechnology and Bioinformatics,
Kuvempu University, Shimoga, Karnataka, India.

3Department of Biotechnology and Microbiology, Kanatak University, Dharwad,
Karnataka India
ABSTRACT
Plant based medicines are effective against many human infectious diseases either by
paralysing or killing the pathogen. In the present study, petroleum ether, chloroform,
ethanol and aqueous extracts of Alstoniascholaris bark were screened for their bactericidal,
fungicidal and anthelmintic properties. Antibacterial activity revealed that chloroform
extract at the 20 mg/ml showed significant antibacterial effect. Nevertheless, petroleum
ether, ethanol and aqueous extracts also showed antibacterial effect against E. coli and S.
dyscentreae, but less effective than chloroform extract. All the extracts were not as potent
as the standard drug ciprofloxacin. Fungicidal activity revealed that among all the test
extracts, ethanol extract at 20 mg/ml showed significant fungicidal effect against Rhizopus. .
Interestingly, petroleum ether and ethanol extracts at 20 mg/ml showed more significant
fungicidal action when compared to standard drug sulphamethoxazole. Anthelmintic
activity of A. scholaris extracts was carried out at four different concentrations viz., 2.5, 5.0,
10.0and 20.0 mg/ml to evaluate their effect in inducing paralysis and death in
Pheretimaposthuma. Anthelmintic activity revealed that petroleum ether extract at 20
mg/ml induced paralysis in worms within 12 min and death within 25.33 min. However,
chloroform, ethanol and aqueous extracts at 20 mg/ml also showed significant
anthelmintic activity. Among all the extracts of A. scholaris, chloroform extract was most
potent at concentration 20 mg/ml but less efective than standard drug albendazole. This
investigation revealed that all the extracts of A. scholaris showed efficient bactericidal,

fungicidal and anthelmintic activity against the test pathogens indicating the medicinal
property of A. scholaris.
KEYWORDS: Bactericidal activity; Fungicidal activity; Anthelmintic activity; Alstonia.
PP-LS-22
ISOLATION, PURIFICATION AND CHARACTERIZATION OF PROTEASE .
Shivaji Bole*1., Prity Lata1., Madhu Singh R1.
1Dept. of Biotechnology, The Oxford College of Science, 19 th Main, 17th B cross, HSR Layout,
Bangalore.
*Shivaji.B.Associate Professor, Department of biotechnology,
The Oxford College Of Science, Bangalore, Karnataka-560102
Email:shivajibio@gmail.com, +91 9611922895, Fax: (080) 30266349
ABSTRACT
Among different types of enzymes obtained from microbial sources, protease are the most
widely used in industries. In the present study, bacteria were isolated from air exposure
and screened for the production of protease. The bacterial isolate was identified as Bacillus
sp. and was later used for further characterization. Maximum yield of Protease was
obtained after 48hrs of incubation. Enzyme activity of Protease was found to be
0.7917µmol/ ml/min and specific Activity was found to be 0.425 µmol/ mg/min. . The
optimum temperature for enzyme activity was found to be at 30 0C and the optimum pH for
enzyme activity was found to be at pH 7.6.
Keywords: Protease, Casein, Cultural characterization, protease activity, pH, Temperature.
PP-LS-23
NOVEL DIAGNOSTIC METHOD FOR TUBERCULOSIS
Solanki Mukherjee, Bhanushree Das, Sevenky Sver, Praveen Kumar, Prajeesh P.
The Oxford College of Science, 19th Main, 17th B cross, HSR Layout, Bangalore.
ABSTRACT
M. tuberculosisis an acid fast bacilli. When stained by Ziehl-Neelsen stain, they appear
straight or slightly curved rod, about 3 µm × 0.3µm, occurring singly, in pairs or insmall

clumps when observed under microscope. Selective media of M. tuberculosis is egg based
Lowenstein-Jensen mediumand the Colonies appear luxuriantly and bunch of cauliflower
shape.It is the major cause of tuberculosis. One third of the world population is estimated
to have been infected with Mycobacterium tuberculosis and million new cases of
tuberculosis arise each year. The majority of tuberculosis patient are 15-45 years of age
andnewly emergence of Multi Drug Resistant TB (MDR TB) and Extremely Drug Resistant
TB (XDR TB). Tuberculosis (TB) remains one of the major causes of death from a single
infectious agent worldwide. Hence several measures and diagnostic techniques have been
developed for its detection. Microscopically by Ziehl-Neelsen staining method, culturally by
L.J solid media and Middle-brook broth media (MGIT7H9 BACTEC), molecular techniques
such asGenexpert, LPA(Line Probe Assay) and Immunochromatographic method(CAPILIA).
PP-LS-24
EVALUATION OF FORMONONETIN AND BIOCHANIN A AS SELECTIVEESTROGEN
RECEPTOR MODULATORS (SERM) - POTENTIAL IMPLICATIONS IN THE THERAPY OF
POST-MENOPAUSAL OSTEOPOROSIS.
Sowmya Kumar * and M Sreepriya **
Department of Microbiology and Biotechnology, BangaloreUniversity, JB Campus
Bangalore – 560 056
ABSTRACT
Osteoporosis is a silently progressing metabolic bone disease, targeting mainly the women
population. It is the major cause of morbidity, mortality and significantly affects the quality
and life expectancy of elderly women. Ovarian hormone deficiency is the major risk factor
for decreased bone mass and increased bone fragility in osteoporosis. HRT (Hormone
replacement therapy) is considered as the mainstay treatment for osteoporosis but this is
reported to increase the risk of breast cancer incidence. Other antiosteporotic drugs
available in the market also causes an array of painful side effects. This has stimulated the
interest in the clinical development of SERMs as antiosteoporotic agents. The aim of the
present investigation is to study the effect of Formononetin and Biochanin-A on the
proliferation of primary culture of osteoblasts isolated from the femur and on estrogen
receptor positive human breast adenocarcinoma cell line MCF-7. Cell proliferation was
assessed by MTT assay and cell morphology by phase contrast microscopy analysis. Results
indicate the prostimulatory effects of formononetin and biochanin A on osteoblast cultures
but the absence of the same on MCF-7 cells thereby implicating the possibility that they
could exert tissue selectivity in estrogenic action and could be potential SERMs. Further
studies are ongoing in the laboratory to support the hypothesis.
* Presenting Author: email: sowmyang11@gmail.com Mob: +91973124855.
** Corresponding author
PP-LS-25
PHENOTYPIC AND MOLECULAR EVALUATION OF F 7 TOMATO RIL’S FOR EXTENDED
SHELF LIFE
*Sukanta Sinha,Sujeet Kumar, Mallikarjuna N MandRamanjiniGowda P H
Department of Plant Biotechnology, University of Agricultural Sciences,
Bangalore -65
*corresponding author:sukantaaaa@gmail.com
ABSTRACT
Tomato is the most important crop in term of nutrition, health and economic value. It
experiences great postharvest losses due to natural perishability, precarious
transportation, lack of proper storage condition in Indian situation. In India, losses of up to
40 percent of produce occur because of excessive fruit softening. Among all strategies
available for minimizing the postharvest losses, genetics approach is the best way. Among
genetic approaches, marker assisted breeding is the promising one as transgenic is not
reasonable in India. With the objective of selecting tomato lines with high shelf life F 7 lines
were derived from crosses between L121 × Vaibhav on the basis of shelf-life and fruit yield.
L121 is (alc gene) showing high shelf-life; Vaibhav is having good agronomic cultivar
released by the UAS Bangalore. We have obtained lines which showed high shelf-life more
than 70 days and mean of F7lines were 45 days. These lines were evaluated at molecular
level using SSR Markers and confirmed the presence of marker Tom184 and Tom144
which are linked to high shelf life in tomato. The tomato lines like RIL 7-3, RIL 34-2, RIL
110-2, RIL 182-4 have shown shelf life of 70 days. These lines with high shelf life can be

used further in crop improvement programme to increase the keeping qualityof tomato so
that post-harvest losses can be minimized.
PP-LS-26
SCREENING OF Streptomyces Spp FOR LIPASE INHIBITORS
Sukrutha Suresh, Venkata Krishna Bayineni, Gurmeet Singh and Ravi-Kumar
Kadeppagari
NanoBiosciences, Centre for Emerging Technologies, Jain University, Jain Global Campus,
Jakkasandra post, Kanakapura Main Road, Ramanagara Dist., Karnataka, India, 562112
ABSTRACT
Cases of obesity are increasing at alarming rate in our country due to the improved socio-
economic status of the people. Obesity is primarily related to the lipid metabolism and
enzymes involved with this metabolism can be selectively targeted for developing anti-
obesity drugs. Orlistat is the only commercially available lipase inhibitor for treating
obesity. However, it has adverse side effects like acute kidney injury, gastric problems and
acute oxalate nephropathy. Hence there is a need for lipase inhibitors that has no or
reduced side effects. One way of obtaining new inhibitors is by screening un-explored
species. We have screened un-explored species of Streptomyces for lipase inhibitors since
Streptomyces were shown to produce novel metabolites. The un-explored Streptomyces
species like S.annulatus, S.albaduncus, S.clavifer, S.coelicolor, S.peuceticus etc. were screened
for checking the lipase inhibition activity and S.abraviensis was used as a positive control
while screening since it was already reported to produce lipase inhibitors, Ebelactones. The
species of Streptomyces were grown on the medium containing soluble starch, glucose,
soya bean meal , yeast extract, CaCO 3, NaCl, MgSO4 at 30°C and 140-150rpm for 5-7days.
Biomass was separated from the medium by centrifugation and supernatant was used for
estimating the extracellular lipase inhibition activity. In order to check intracellular lipase
inhibition activity the biomass was macerated using appropriate volume of water and
extracted by using equal volume of ethyl acetate. S. coelicolor showed lipase inhibition
activity in the both extra and intracellular extracts. Inhibition activity was around 65-
100%. Other species didn’t show significant lipase inhibition activity.

PP-LS-27
ORAL PATHOGENS IN HIV INFECTED PATIENTS WITH SPECIAL REFERENCE TO
CANDIDA
Sumalatha.S, AshaNinan
Department of Microbiology,The Oxford College of Science, Bangalore.
ABSTRACT
Candida species are the most common fungal pathogens isolated from the oral cavity. Their
oral existence both as a commensal and an opportunist pathogen has intrigued clinicians
and scientists for many decades, and recent investigations have revealed many attributes
of this fungus contributing to its pathogenicity. . Many factors contribute to the
development of oropharyngeal candidiasis (OPC) including malnutrition, poor oral hygiene,
dental malocclusion, and immuno suppression . In addition, the advent of the human
immunodeficiency virus infection and AIDS has resulted in a resurgence of oral Candida
infections. Therefore, this review is an attempt at detailing the current knowledge on
Candida and oral candidoses.Oral candidiasis, which is also called thrush, is a fungal
infection of the mouth and/or throat.
Till date, CD4 cell count and viral load are recognized and widely used as markers of
HIV-related disease progression. However, laboratory parameters will only partially reflect
disease stage and progression, the additional clinical marker will more accurately
represent the patient’s overall disease status.Considering the individual significance of CD4
cell count and oral lesions in assessing the disease status, it was thought worthwhile to
correlate the two parameters, viz. CD4 cell count and oral lesions, in HIV/AIDS patients.
This study was undertaken to assess the efficacy of oral changes as an indicator of disease
status in HIV/AIDS.
The study included 205 subjects were used for this study. Two samples (blood and oral
wash) were collected from each subject. Each specimen was processed using standard
microbiological techniques and emergent Candida isolates were identified with
CHROMagarTM Candida and germ tube test. Venous blood samples (5 ml) were collected
into ethylene diaminetetraacetic acid (EDTA) containers and mixed. The blood specimens
were used for CD4 counts using flow cytometry following the manufacturer’s
instructions.In patients without oral candidiasis, samples from saliva were collected by oral
rinses in salinefor one min.InoculatedonSabouraud dextrose agar and HiCromeCandida
agar After 48 hours of incubation at 37 °C.Yeast isolates were selected for species level
identification by colony morphology on Sabouraud agar and colony color on HiCrome
Candida agar. In clinical samples with different colonies, one isolate of each colony was
obtained for species identification.Identification of the species of the isolates was
performed by standard methods (germ-tube formation).Direct Examination of the
Specimens (Gram stain and wet mount technique).
Out of 295 patients, 72 people had oral candidiasis,The majority of Candida species among
the Candida isolates were Candida albicans followed by Candida tropicalis ,Candida krusie
and Candida guilliermondii .
PP-LS-28
PROTECTIVE EFFECTS OF RESVERATROL AGAINST Β-ADRENERGIC AGONIST
INDUCED MYOCARDIAL STRESS IN DIABETIC RATS
Uma .S and Sreepriya M *
*Department of Microbiology and Biotechnology, Bangalore University,
Jnana Bharathi Campus, Bangalore – 560 056
ABSTRACT
India is notoriously known as the diabetes capital of the world. Diabetes superimposed
with macrovascular cardiac complications is the major cause of morbidity and mortality
worldwide. Till today its management remains a clinical challenge and a problem of great
importance. Despite understanding the mechanisms contributing to the development of
cardiovascular diseases in diabetic patients, currently available conventional treatments
for preventing the same are severely limited. Hence there is increased interest and
renewed research focus on investigating the potential of natural products in the prevention
of cardiovascular disease during diabetic conditions through novel strategies. Phytoalexins
are chemcial substances produced by plants as a defensive response against infection by
pathogenic microorganisms such as fungi. Resveratrol ( 3,4,5 trihydroxystilbene) is a
naturally occurring phytoalexin reported to possess antidiabetic and cardioprotective
properties. Scientific studies implicating the effects of resveratrol in preventing the

cardiovascular complications during diabetic conditions are lacking. Hence the present
investigation was carried out to study the effects of resveratrol supplementation (5mg/kg
body wt/day orally for 21 days) to combat myocardial stress induced by isoproterenol
(150 mg/kg body wt single i.p) in diabetic rats. Biochemical and pathological analysis were
performed to evaluate the same. In vitro studies were carried out on cardiomyocytes to
complement the in vivo findings. Results confirm the protective effects of resveratrol
treatment in abrogating isoproterenol-induced cardiac damage during diabetogenic
conditions both in vivo and in vitro.
PP-LS-29
Couroupita guianensis FLOWER EXTRACT (NAGALINGA PUSHPA): A POTENTIAL
NATURAL REMEDIES FOR DENTAL CARIES
Umesh R, Vijaya R, Bhanuprakash K S, and Dr. Sangita Roy.
Department of Biochemistry, The Oxford College of Science, Bangalore, Karnataka.
Dental caries are one of the most common chronic infectious diseases in the world.
Bacterial plaque accumulated on dental surfaces and composed of native oral flora are the
primary etiologic agent of dental caries. It is a major health problem and comprises 60-
90% children and vast majority of adult. Herbal remedies have a long history of use for
gum and tooth problems such as dental caries. In vitro studies indicate that a variety of
common oral bacteria are especially sensitive to the antimicrobial activities of many plant
extract. Couroupita guianensis, commonly named as Cannonball tree, belong to the family
Lecythidaceae is an indigenous tropical rainforest tree of South America and India. The
antimicrobial, anti-inflammatory properties of the flower of Couroupita guianensis
(nagalinga pushpa) have already been reported. In Ayurveda, nagalinga flower extracts
were used as a pain reliever for dental diseases, but there is no such report of its role in
dental caries and other periodontal diseases. This study is aimed to elucidate the function
of Couroupita guianensis flower as a potential candidate for in vitro treatment of dental
caries and a future natural medicine for dental caries.

PP-LS-30
ISOLATION AND SCREENING OF HEAVY METAL RESISTANT BACTERIA
Usha M. Subbaiah*, Harshitha S. Chandrakumar and Harshitha Badrinath
Department of Microbiology, CPGS, Jayanagar, Jain University,

Bangalore 560011, India.
Email: bg.ushams@gmail.com, Ph. No. +91 8041210691, Fax. +91 8041210692.
ABSTRACT
Out of 6 samples collected from different areas of Peenya around 55 isolates were obtained.
All these 55 isolates were tested for resistance against 5 different metal salts i.e. Cadmium
acetate, Ferrous sulfate, Mercuric chloride, Lead acetate and Zinc sulfate at 0.1%
concentration. All the 55 isolates (100%) showed complete resistance to Ferrous sulfate.
Similarly 51 isolates (93%) showed complete resistance to Zinc and 4 (7%) isolates
showed 8 mm diameter of zone of inhibition. When resistance against Lead was checked
44 (80%) isolates showed complete resistance and 11 (20%) isolates showed 10 mm
diameter of zone of inhibition. Among 55 isolates, 3 (5%) isolates showed complete
resistance to Cadmium; 6 (11%) isolates showed 10 mm diameter of zone of inhibition
against Cadmium, the rest 46 (84%) isolates showed diameter of zone of inhibition
between 10-41 mm. Five (9%) out of 55 isolates showed 11 mm diameter of zone of
inhibition against Mercuric chloride and the rest 50 (90%) isolates showed 12-40 mm
diameter of zone of inhibition. The isolates JUHc1 and JUHd5 showed 10 mm diameter of
zone of inhibition against Cadmium and Mercury, while complete resistance was shown
against Lead, Ferrous and Zinc. Hence these two isolates have been selected for further
studies.
Keywords: Heavy metal, resistant, Bacteria

PP-LS-31
SEROPREVALENCE SURVEY OF RUBELLA INFECTION AMONG FEMALES OF
REPRODUCING AGE (15-40) YEARS ATYTENDING RIMS, RANCHI
Anupama Kumari, Suprava Sona, Natasha Rawat
Department of Microbiology, The Oxford College of Science
ABSTRACT
Clinical or subclinical infection of rubella in pregnant mother gives rise to a handicap baby
to the society. Rubella is normally a self limiting febrile illness without significant long term
morbidity. Infection during pregnancy, may lead to miscarriage, fetal death or the birth of
an infant with congenital rubella syndrome (CRS).It is estimated that worldwide more than
100 000 children with CRS are born each year.single blood sample was taken from 120
females of reproducing age (15-40) years visiting RIMS hospital over a period of one
month. All the sera was examined by Sandwich enzyme linked immunosorbant assay
(ELISA) for rubella specific IgG and IgM and Chemiluminescence technique and compared
the techniques for its efficiency. A questionnaire was used to collect demographic data
including age, sex, previous history, previous vaccination and knowledge about rubella.
Among 120 females tested for rubella infection, 95 of them was found positive for rubella
IgG, representing the high level of exposure to this infection. Around 43 females were
positive for IgM, representing the recent infection. And 20 of pregnant females were found
to be IgM positive in the first trimester and thus a high risk of CRS. This pilot study done in
one month in 120 females represent most of the district of Jharkhand. It was found that the
percentage of IgG positive females were more in Jharkhand compared to Delhi and Kashmir
based on the article referred. This shows low immunization coverage rate in Jharkhand.

PP-LS-32
AGRO WASTE AS SOURCE OF ECO-FRIENDLY NATURAL DYE FOR SILK
V. Narayanaswamy1, K.N. Ninge Gowda2 & R. Sudhakar2

1Dept. of Fashion & Apparel Design, The Oxford College of Sciences, Bangalore, India
2Dept. of Studies in Apparel Technology & Management, Bangalore University, Bangalore,
India
Corresponding Author: gvnnarayan@gmail.com
ABSTRACT
Dyeing component has been extracted from the bark of Swietenia macrophylla for its
application on natural textile substrates viz., cotton, silk and wool. Color values and color
co-ordinates were examined. The extracted dye showed excellent wash, rub and
perspiration fastness and good light fastness on all the substrates. The dye was tested for
some of the eco-parameters using atomic absorption spectrophotometer and Gas
Chromatography/Mass Spectra. The test results were compared with the set standards to
determine the eco-friendliness of natural dye. Their concentrations were much below than
the stipulated limits. Dyed samples were also tested for antimicrobial activity against gram-
positive and gram-negative bacteria and are found to possess antibacterial activity. The dye
may be considered as an eco-friendly alternative for dyeing natural textile fabrics.
Key words: antimicrobial activity, dyeing, heavy metal, Swietenia macrophylla, textile.

PP-LS-33
ANTIDERMATOPHYTIC PROPERTIES OF TURMERIC OIL, AND CURCUMA LONGA

PREPARATIONS
Suhasini Badigar
Department of Microbiology, the Oxford College of Collge
ABSTRACT
Curcuma longa L. or turmeric of the family Zingiberaceae is widely used in Thai traditional
medicines for the treatment of rash, itching, tinea, and ringworm. Previous studies on
turmeric oil reported effective antifungal activity against dermatophytes, a group of fungi
that causes skin diseases. In this study, turmeric creams containing 6 and 10% w/w
turmeric oil were prepared and tested against clinical strains of dermatophytes using broth
dilution technique. Minimum fungicidal concentrations of 6 and 10% w/w turmeric creams
were found to be 312 μg/mL. Ar-turmerone, a major compound separated from turmeric
oil, promoted more effective antidermatophytic activity with the MICs of 1.56–6.25 μg/mL,
compared to 3.90–7.81 μg/mL of standard ketoconazole. The results indicated that
6% w/w turmeric oil in the cream was suitable to be formulated as antidermatophytic
preparation. Further research should be done on long-term chemical and antifungal
stabilities of the preparation.

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Email: aegroups@yahoo.co.in
Mob: 9448133690, 9741117179, 080-23640735
With Best Compliments from………….
Shri. H. K. Raja Gopal (Rajanna)

Chikkanayakanahalli, Cormalaran Post, Bengaluru – 560 035

Systronics India Limited

No. 146/4, Shantala Plaza,
II floor, 8th Main, 14th Cross, Malleshwaram,
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JNANARJANA 2K14, 6 th National Scinece Conference on “Beyond the frontiers in Science & Technology”

The Oxford College of Science, Bangalore 1

ABOUT BANGALORE CITY

ABOUT THE CONFERENCE

The Oxford College of Science, Bangalore 3

ABOUT THE OXFORD COLLEGE OF SCIENCE

The Oxford College of Science, Bangalore 4

The Oxford College of Science, Bangalore 5

Estd. 1974 Ph: 26630855,

CHILDREN’S EDUCATION SOCIETY (Regd.)

It gives me immense pleasure to acknowledge Jnanarjana 2K14, 6 th

Convergence of all the fields of science on a single platform for the

The Oxford College of Science, Bangalore 6

Estd. 1974 Ph: 26630855,

CHILDREN’S EDUCATION SOCIETY (Regd.)

On the occasion of the 6th ‘Jnanarjana’, National Conference on

S.N.V.L. NARASIMHA RAJU

The Oxford College of Science, Bangalore 7

Estd. 1974 Ph: 26630855,

CHILDREN’S EDUCATION SOCIETY (Regd.)

I congratulate the faculty and students of The Oxford College of Science

The Oxford College of Science, Bangalore 8

The Oxford College of Science, Bangalore 9

COMMITTEE IN-CHARGE MEMBERS

Programme Committee Ms.Vaidhehi Jois , Ms. Deepti Varier & Ms.Muthamma

Registration Committee Mr Prajeesh P, Ms. Sonhita & Ms Asha Ninan

Speaker Hospitality Committee Ms Madukala , Ms Vatsalya Krupa, Ms.Pallavi

Stage Committee Ms Vijaya, Ms.Mamata, Ms Deepa Kumari, Ms

Accommodation Committee Dr.Prakash, Mr.Nagaraju, Dr.Arpita, Ms.Lakshmi Priya,

Food Committee Dr Narayana Swami, Mr Veerendra, Ms.Indu, Ms.Swetha,

Printing Committee Mr Sekar.K.V & Dr.Pramod T

Cultural Program Committee Ms. Pavana, Ms. Gayathri

Scientific Committee Dr.Abdul Nayeem, Mr.Ramanuja H S, Mr.Prajeesh.P,

Ms.Chetana Heroor, Ms.Pavana, Ms.Shantala, Ms.Sathya

The Oxford College of Science, Bangalore 10

Abstract No. Title of Abstract

The Oxford College of Science, Bangalore 11

OP-LS-18 Prophage Mediated Sero-Divergence in Salmonella Species.

Comparative Morphometric analysis Of Different Species of Termites

Studies on Low Cost Medium in Production of Chlorella Species Using Cow

Antiquorum Sensing Activity of Quercetin against Multidrug Resistant

The Oxford College of Science, Bangalore 12

Abstract No. Title of Abstract

Leaf Litter Mycoflora of Some Medicinal Plants from Bangalore Rural

PP-LS-17 Antibacterial Studies Of Caesalpinia Bonducella Seed Extracts

The Oxford College of Science, Bangalore 13

ELISA- Best Advanced Handy Detection Test of Picking the

The Oxford College of Science, Bangalore 14

The Oxford College of Science, Bangalore 15

IMPACT OF CIGARETTE SMOKE ON HUMAN PERIPHERAL BLOOD LYMPHOCYTES –

Anupama Singh, Uthayasuriya, Anjali , Bhavya and Sreepriya M *

The Oxford College of Science, Bangalore 16

Jyothi S. C., Akshatha Avadhani, Surendra N. S.

The Oxford College of Science, Bangalore 17

Sumalatha.K.R and M.Sreepriya*

The Oxford College of Science, Bangalore 18

FABRICATION AND ANTIPSEUDOMONAL EVALUATION OF PLANT EXTRACT LOADED

Adheena K.S 1, Ibrahim V 1, Asha ninan 1, Jayachandran V.P 2

Chitosan (CH) is a natural mucopolysaccharide of marine origin consists of a linear (1-4)

The Oxford College of Science, Bangalore 19

concentrations of CH (4% 6% 10%). On freeze drying, using more concentrated CH

ETIOLOGY OF ORGANISMS IN ICU IN TERTIARY CARE HOSPITAL AND ITS ANTIBIOTIC

Ahuti Debasmita, Amrita Raj, Basim Sufi, Prajeesh P