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Energy
excited states.
• The particular frequencies at which light
Ground state
n 258 nm
e
r 217 nm
175 nm
g
y
Assumptions:
Wavelength of transition for
Anthracene
in box of length L.
Ignore all coulomb interactions.
L
L6A
Pigments and Quantum mechanics
I0 I
• Various designs of spectrophotometers : Fixed and variable wavelength; Single, split and
double beam
• Cuvettes: Glass or plastic – visible
Quartz – visible, UV
Acrylic – visible, UV
Monochromator
Monochromator
Instrumentation
Absorbance and the Beer – Lambert Law
According to the Beer-Lambert Law, absorbance
is proportional to concentration, and Absorbance
versus concentration plot is a straight line.
Deviation from Lambert’s Beer Law
Electronic levels and transitions
N
Unoccupied
p
levels
N p
N
p
p
Occupied
levels
p
p
σ→σ* Alkanes