STUDY OF ANTIBACTERIAL

ACTIVITY OF DIFFERENT PARTS

OF TARO PLANT (Colocasia esculenta)
 A RESEARCH PAPER IS SUBMITTED TO THE

UNIVERSITY OF MUMBAI

FOR THE DEGREE OF

BACHELOR OF SCIENCE (B.Sc.) IN BIOTECHNOLOGY

STUDY OF ANTIBACTERIAL
ACTIVITY OF DIFFERENT PARTS
OF TARO PLANT (Colocasia esculenta)
SUBMITTED BY
MS. RASHMI SANTOSH PANDEY
UNDER THE GUIDANCE OF
MISS. SEEMA D JADHAV

DEPARTMENT OF BIOTECHNOLOGY

P.A.J.B.S.UNNATI MANDAL̓S B.N.N COLLEGE OF ARTS,

SCIENECE AND

COMMERCE

BHIWANDI-421305

(2018- 2019)
 CERTIFICATE
P.A.J.B.S.U. MANDAL’S B.N.N COLLEGE OF ARTS

SCIENCE AND COMMERCE BHIWANDI -421302

This is to certify that Ms. Rashmi Santosh Pandey Of B.S.C Biotechnology,

Seat No. has satisfactorily completed the dissertation on “ STUDY
OF ANTIBACTERIAL ACTIVITY OF DIFFERENT PARTS OF TARO
PLANT (Colocasia esculenta)” as prescribed by the University of Mumbai,
during academic year 2018-19. The above entitled project is enclosed here with
for evaluation at the Annual University Practical Examination.

Date:

Project guide Examiner

Miss. Seema D.Jadhav

Head of Department

Archana Tajane

College Stamp
 DECLARATION

I, here declare that project entitled “STUDY OF ANTIBACTERIAL ACTIVITY

OF DIFFERENT PARTS OF TARO PLANT (Colocasia esculenta)” Submitted
for the partial fulfillment of BSC Biotechnology, BNN college Bhiwandi, is
carried out by me, Department of Biotechnology, under the Guidance of Miss.
Seema Jadhav, lecture, Department of Biotechnology, BNN College Bhiwandi.

DATE

PLACE: BHIWANDI

MISS. RASHMI SANTOSH PANDEY

 ACKNOWLEDGEMENT
I am very thankful to our principal, Dr.Ashok wagh and to the management of B.N.N.
college, bhiwandi for providing me all the facilities during the entire course of this
project.

I owe a deep gratitude to Miss. Archana Tajane, Head department of Biotechnology

for their cooperation and guidance.

I am thankful to my guide Miss. Seema D. Jadhav for putting in her valuable time
and taking interest in my difficulties. Her perfectionist attitude has taught me to never
to settle for anything less than perfect. Without her constant encouragement and
valuable guidance this project work could not have been completed.

A million thanks to all my friends and teacher who helped me in my project work that
has been a very informative and enjoyable experience, for which I am immensely
grateful to them.

There are no words to express my feelings of gratitude and appreciation towards my

family. Without their support, trust and help nothing would have been possible.
Thanks for encouraging me to stay focused during their highlights and lowlights of
my work.

I thank almighty for blessings endowed on me.

Rashmi Santosh Pandey

 INDEX

Sr. No. Title Page No.

List of tables

List of plates

1. Introduction 1

Taro plant (Colocasia esculenta) 1

Antibacterial activity 5

2. Review of literature 8

3. Aims and objectives 12

4. Materials and methods 13

Taro plant extract preparation 16

Agar Well diffusion method 17

5. Results and discussion 18

6. General conclusion 25

7. Bibliography 27
 List of Tables
Sr. No Table Content Page
No
Table 1 Dilution table for extracts of three parts of Taro plant 17

Table 2 Antibacterial activity of Taro leaf extract (distilled water 18

and methanol) by agar well diffusion method on E.coli

Table 3 Antibacterial activity Taro leaf extract (distilled water 18

and methanol) by agar well diffusion method on s.aureus

Table 4 Antibacterial activity of Taro stalk extract (distilled water 20

and methanol) by agar well diffusion method onE.coli

Table 5 Antibacterial activity of Taro stalk extract (distilled water 20

and methanol) by agar well diffusion method on S.aureus

Table 6 Antibacterial activity of Taro tuber extract (distilled 22

water and methanol) by agar well diffusion method on
E.coli

Table 7 Antibacterial activity of Taro tuber extract (distilled 22

water and methanol) by agar well diffusion method on
S.aureus
 List of plates
Sr. No Name of plate Page
No.
Plate 1 Taro plant (Colocasia esculenta) 1

Plate 2 Taro tuber 7

Plate 3 Taro leaf 7

Plate 4 Taro stalk 7

Plate 5 Taro leaf (blended sample) 13

Plate 6 Taro stalk (blended sample) 14

Plate 7 Taro tuber (blended sample) 14

Plate 8 Extracts of three parts of taro plant 16

Plate 9 Plate results for Taro leaf water extract on E.coli and 18
S.aureus

Plate 10 Plate result for Taro leaf methanol extract on E.coli and 18
S.aureus

Plate 11 Plate results for Taro stalk water extract on E.coli and 19
S.aureus

Plate 12 plate results for Taro stalk methanol extract on E.coli and 19
S.aureus

Plate 13 Plate results for Taro tuber water extract on E.coli and 20
S.aureus

Plate 14 Plate results for Taro tuber methanol extract on E.coli and 23
S.aureus