5: Microbiology | Gram-Negative Bacilti Questions 161 An organism isolated from the surface of a skin burn is found to produce a diffusible green pigment on a blood agar plate. Further studies of the organism would most likely show the organism to be: a Staphylococcus aureus b Serratia marcescens Flavobacterium meningosepticum Pseudomonas aeruginosa 162 A nonfermenting gram-negative bacillus is isolated from @ wound. The nitrate and oxidase are strongly positive. The growth on sheep blood agar has a grape-like odor. The organism is: Burkholderia cepacia Moraxella lacunata Chryseobacterium (Flavobacterium) meningosepticum Pseudomonas aeruginosa aaoe 163 A small, gram-negative bacillus is isolated from an eye culture. It grows only on chocolate agar and is oxidase-negative. The most likely organism is a Acinetobacter bwoffi b Haemophilus influenzae € Stenotrophomonas maltophilia Pseudomonas aeruginosa 164 A blood culture bottle with macroscopic signs of growth is Gram stained and the technician Shy notes small, curved gram-negative bacilli resembling “gull wings.” It is subcultured to blood and chocolate agar, and incubated aerobically and anaerobically. After 24 hours, no growth is apparent. ‘The next step should be to: subculture the bottle, and incubate in microaerophilic conditions assume the organism is nonviable, and ask for repeat specimen utilize a pyridoxal disk to detect Aeromonas subculture the bottle to a medium containing X and V factors ane 165. The optimal incubator temperature for isolation of the Campylobacter jejuni/coli group is: a 4c b 20°C © 25°C a 47°C 166 A patient with a nosocomial pneumonia has a sputum Gram stain that shows many neutrophils {iy and numerous small gram-negative coccobacilli. The organism grew in 24 hours as a mucoid, hemolytic colony on blood agar and a colorless colony on a MacConkey agar. The organism had the following characteristics: oxidase: negative calalase, positive nitrate: negative ‘ONPG: negative ornithine decarboxylase: negative lysine decarboxylase: negative The organism is a Stenotrophomonas maltophilia b Alcaligenes faecalis € Moraxella lacunata Acinetobacter baumannii 306 The Board of Certification Study Guide