1 Abstract

The goal of the Transpiration lab is to witness transpirations as it take place in a natural setting.

The lab ran four treatment under (1) normal conditions, wind treatment, and humidity

treatment. Each treatment is to show how plant life is more or less affected by water circulation

(transpiration). The treatment took place in the lab and at the end, circulation revealed that less

change occur under each treatment (Rep), and that could be the case, because heat is a major

factor in the environment that affects plants more readily than other factors. But, I stand to

reason that the result would be more substantial under a more calibrated natural treatment.

2 Introductions

Transpiration is the process through which plants loose water. Plants lose water through

different conditions: Wind, Heat, and Humidity. Stomata openings are intrinsic to the

transpiration processes. Stomata’s are minute opening by guard cells in the epidermis of leaves

and stems. There are different aspects of water transpiration throughout the body of plan:

cohesion, adhesion, and tension. Specifically this lab testing Transpiration on Zabrina plants

under four specific treatments: Room temperature, simulated Winds, increase Humidity, and

Heat treatment. As an hypothesis, transpiration will occur especially with Humidity and Heat

treatment.

3 Materials and Method

The group were broken up in two’s (pairs). We are to determine the rate of transpiration under

room temperature. I particularly worked in testing “Wind treatment”. Thus, the wind was

simulated by placing the plants 2 meters away from the fan and the fan is set to medium

settling. Another pair working with humidity ( vapor pressure), sprayed the plant with water
and covered it with a plastic bag. And finally, the temperature of both the leaf and the air were

increased, as well as the light intensity by placing the plant some few meters away from the

flood lamp, somewhere between (1.25 to 1.5m).

4 Results

The result demonstrates that transpiration does occur under these condition, more in some

conditions than the others. Under normal treatment or room conditions, transpiration fluctuates

as changes begin to occur after 20mil ml of 0.010 to 30 minutes ml with 0.01 , averaging 0.004

per hour. Wind treatment did not experience much water vapor loss, in both (Rp1) and in

(Rp2), conditional changes were minimal, according to calculations average water loss were

-.166 per 10 minutes with a difference of 0.01 (Rep1) and .24 per hour. Such was the case in

(Rep2). Heat treatment also did not experience much water transpiration in (Rep1). But, in

(Rep2), there was a significant change after 20min ml with 0.02 to 0.035 in 30 minutes ml to

0.047 in 40 minutes ml to 0.051 in 50min. Much of this is affected by the leaf mass. Finally,

the Humidity treatment, which stayed constant, zero changes. This test prove my hypothesis

wrong, under these conditions : Humidity and Heat treatments. But I stand to reason that my

hypothesis would be more, rather than partially correct if the test were carried out under more

natural settings.

5 Discussions

Considering the result of this transpiration lab; it did partially support my hypothesis, which

states that transpiration is bound to take place with two specific treatment( constant). As

supported by the result: Heat treatment (Rep2) changes significantly on the average of 0.615

per hour. Interestingly, Heat treatment had the most significant change as compared to normal

treatment and the rest of the treatment ( again this is due to the fact that Heat is a significant
 atmosphere factor that controls our environment. This lab is modeled after two specific

resources: (1) Effects of leaflet orientation on Transpiration Rates and Water potential of

Oxalis Montana, and (2) Enhancement of transpiration by ethylene is responsible for

absence of elongation in floating rice at low humidity. These two resources are excellent

examples of how transpiration affects the growth and life of plants across the world.

6 Resources

(1) Comerro, K., Briggs, G. (2003). Effects of leaflet orientation on Transpiration rates and

Water potential of Oxalis Montana.

(2) Azuma, T., Hatanaka, T., Uchida, N., Yasuda, T.(2003). Enhancement of transpiration

by ethylene is responsible for absence of elongation in floating rice at low humidity. J.

Plant Phisiol

(3) Helms, D., Helms, C., Kosinki, R., Cummings, J. (2003). Water movement and mineral

Nutrition in plants. Biology in the Laboratory, 3e (pp 143-156). Jacksonville: W.H.

Freeman and company.

(4) Solomon, E., berg, L., Martin, D. (Eds). (2002). Leaf structure and Function. Biology

6e (pp 692-748). United States: Thompson learning, Inc

7 Graphs
 Transpiration experiment Section 88

Normal treatment

Rep1 Team: Rachel, Robert

mL at start 10min mL 20min mL 30min mL 40min mL 50min mL Leaf mass ( avee/10 ave/hr
0 0.010 0.010 0.01 0.015 0.02 0.175 0.004 0.024

find
difference

Rep2 Team: Lori,

mL at start 10min mL 20min mL 30min mL 40min mL 50min mL Leaf mass ( avee/10 ave/hr
-0.16 -0.15 -0.14 -0.14 -0.14 -0.14 2.81

find
difference

Wind treatment

Rep1 Team: Josh & Chris

mL at start 10min mL 20min mL 30min mL 40min mL 50min mL Leaf mass ( avee/10 ave/hr
-0.2 -0.18 -0.17 -0.17 -0.16 -0.15 0.175 -0.166 0.996

difference 0.18 0.01 0 0.01 0.01 0.01

Rep2 Team: Zuriel & Charlie

mL at start 10min mL 20min mL 30min mL 40min mL 50min mL Leaf mass ( avee/10 ave/hr
1 0.9 0.85 0.85 0.85 0.85 5.75 0.04 0.24

Heat treatment

Rep1 Team: Mary Bonser and Bianca Sandu

mL at start 10min mL 20min mL 30min mL 40min mL 50min mL Leaf mass ( avee/10 ave/hr
2 1.8 1.8 1.7 1.7 1.6 1.69

Rep2 Team: Tiffany Tucker Tu Anh Nguyen

mL at start 10min mL 20min mL 30min mL 40min mL 50min mL Leaf mass ( avee/10 ave/hr
0 0.01 0.02 0.035 0.047 0.051 1.74 0.10 0.615

Humidity treatment

Rep1 Team: Sarah & Alana

mL at start 10min mL 20min mL 30min mL 40min mL 50min mL Leaf mass ( avee/10 ave/hr
0 0.01 0.01 0.01 0.01 0.10 2.42 0.025 0.015
 Rep2 Team: Joe Scarpinato
mL at start 10min mL 20min mL 30min mL 40min mL 50min mL Leaf mass ( avee/10 ave/hr
1 1 1 1 1 1 2.35