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2- suitable medium consistency From a liquid medium can change its consistency
by adding products such as albumin, gelatin or agar, which would obtain media
semisolid or solid state.
3- presence (or absence) of oxygen and other gases lot of bacteria can grow in an
atmosphere with normal oxygen tension . Some may get oxygen directly from
various substrates. But strict anaerobes only properly developed in an atmosphere
without oxygen environment .
5- Most microorganisms grow much better in the dark than in the presence of
sunlight . There are obvious exceptions as in the case of photosynthetic
microorganisms.
8- Sterility of media All culture media must be perfectly sterile to prevent the
emergence of life forms that may alter , mask or even prevent normal or specimens
in the media inoculated microbial growth.
semisolid media They are prepared from liquid media , adding a solidifying agent
thereto in a minor proportion to prepare solid media . One of its uses is
investigating the mobility of bacteria.
ACCORDING TO USE :
1) Common means: Those who possess the minimum components for the growth
of bacteria that do not need special requirements can be produced
2) Media enrichment: Those who, in addition to the normal nutrients, incorporate a
number of essential factors for the growth of fastidious microorganisms. This
enrichment is made by addition of blood or other biological products (blood, serum,
milk, egg, bile, etc.) that provide such factors
3) Selective media: media are used to promote the growth of certain bacteria in
polymicrobial population. The foundation of these means is nutritionally facilitate
the growth of a specific microbial population. An example of selective medium is
selenite, which is used to promote the growth of salmonellae
6) Means of identification: They are designed to test a specific quality that can help
us to recognize the identity of a microorganism. These means must possess the
necessary elements to ensure the growth of microorganisms, the specific substrate
that will be metabolised and the indicator showing us the result.
8) Media storage: Used to keep a strain for various reasons keep us interested.
Mainly they used as quality control tests and reagents used in elLaboratorio
Microbiology. In the laboratory strains can be preserved in three ways:
a) making periodic passes from plate to plate,
1) Complex Media : They were the first used , and the employees are prepared
from animal tissue , and rarely vegetables.
2) synthetic Media : Those containing in its composition only known chemicals and
dissolved in distilled water in certain proportions , resulting in an average of well
defined composition.
3) semisynthetic Media : The large number of growth factors required for certain
germs makes manufacture of a synthetic medium for these organisms is
impossible or too expensive . In this case the growth factors in the form of a
complex organic extract (yeast extract , tissue extract , etc. ) is provided.
BY ORIGIN :
Agar Hektoen: It is a differential and less selective than the SS agar medium. It is
used to facilitate isolation of enterobacteria. The presence of three sugars (lactose,
sucrose and salicin) can extend the differential power of this medium. This medium
is capable of detecting germs SH2 trainers,
C.P.S. ID3. : Chromogenic medium developed by Biomerieux, which allows the
identification of E. coli, P. E. faecalis mirabilisy with simple visualization of the color
change of the colony on the medium (burgundy red, brown blue A).
Agar Trypticase Soy: It is a medium used for the growth of fastidious germs, such
as Brucella, Neisseria or Streptococcus. It is a medium highly enriched, but not
differential. Thioglycollate broth with resazurin: It is a recommended for medium
sterility controls. It allows the cultivation of aerobic, microaerophilic anaerobiosy. It
has a low redox potential that increasing, is manifested by a medium pink.
Chapman agar or saline mannitol salt agar or acronym MSA (Mannitol salt agar)
Mannitol is a culture medium which is normally used in microbiology. It allows the
growth of a particular group of bacteria while inhibiting the growth of others. This
medium is important in the clinical laboratory because it is able to distinguish
pathogenic microorganism in a short time
Baird-Parker Agar: Allows the growth of coagulase-positive staphylococci, while
the difference from the rest.
Loeffler medium: It is a specific medium for the culture of Corynebacterium
diphtheriae.
c ) Salmonella typhi causes typhoid fever glucose (but not lactose ferments ) , does
not produce gas, produce H2S Far Far inferior.- superior.- Red Black and Yellow
e) Escherichia coli is the most common bacteria of the intestinal flora ferments
glucose and lactose , produces gas , and no H2S superior.- Far End Yellow Yellow
inferior.- elevation means
Schaedler Agar : A blood medium and vitamin K very suitable for growing
anaerobes.
Gardnerella Agar : Agar with human blood over a mixture of antibiotics that allow
observation of beta-hemolytic colonies Gardnerella vaginalis Campylosel
characteristics : It is used for isolation of intestinal Campylobacter growing at 42 °
C in microaerophilic .
Agar BCYE (Buffer Charcoal Yeast Extract ): Used for the isolation of Legionella
bacteria
Lowenstein Jensen : This is a basis for the preparation of various media for the
isolation , cultivation and differentiation of mycobacteria.
Bibliography