Document : Standard Test Procedure Date : 01.04.

2013
Department/Function: Quality Rev. No. : 00
Doc. No: YCSR/QD/ STP-01 Page 1 of 6
Title : STP- Determination of the Polarization of Raw Sugar

The Determination of the Polarisation of Raw Sugar by Polarimetry – ICUMSA

Method GS1/2/3/9-1(2011)

1. Field of Application

This method may used to determine the Polarization value of raw sugar based on the
purchasing contracts

2. Reagents required

a) Basic Lead Acetate – Dissolve 560g of basic lead acetate in 1liter of distilled water. Boil
for 30 minutes and allow to settle. Decant the supernatant liquid and dilute to a
density of 1.24 g/ml or 24.2g PbO/100ml with recently boiled water.

3. Apparatus required

Analytical balance readable to 1 mg

Polarimeter
100ml Standard flasks- Class A
Beakers

4. Procedure

4.1 Weigh out accurately 26.000gms of raw sugar sample

4.2 Quantitatively transfer the sample in to 100ml standard flask by adding 60 to 70ml of
distilled water.

4.3 Add basic lead acetate solution according to the expected polarization values. Below
99.3°Z use 1.00ml and above 99.3°Z use 0.50ml.

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 Document : Standard Test Procedure Date : 01.04.2013
Department/Function: Quality Rev. No. : 00
Doc. No: YCSR/QD/ STP-01 Page 2 of 6
Title : STP- Determination of the Polarization of Raw Sugar

4.4 Mix the solution by gentle swirling add water until the bulb of the flask is full.

4.5 Allow to stand for at least 10minutes to attain room temperature. The laboratory
temperature to be controlled at 20 ± 1°C

4.6 Makeup the volume and shake thoroughly.

4.7 Insert a clean dry thermometer in the flask and record the temperature tm

4.8 Allow to stand the solution for 5minutes to settle precipitate

4.9 Filter the solution through whatman 91 filter paper.

4.10 Discard the first 10ml of filtrate and collect 50 to 60 ml filtrate.

4.11 Rinse the pol tube at least twice with the solution to be used and fill in such a way
that no air bubbles are entrapped.

4.12 Place the tube in the polarimeter and close the compartment & record the reading
Ptr.

4.13 Measure the temperature of the solution in the tube tr

4.14 Record the polarimeter reading in air Pa

4.15 Record the standard quartz control plate reading Qtq

4.16 Record the temperature of the quartz control plate tq

4.17 Record the polarimeter reading, pol tube filled with water Pw
4.18 Calculate the polarization corrected to 20°C by applying the below mentioned
formula.

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 Document : Standard Test Procedure Date : 01.04.2013
Department/Function: Quality Rev. No. : 00
Doc. No: YCSR/QD/ STP-01 Page 3 of 6
Title : STP- Determination of the Polarization of Raw Sugar

4.19 Express the results to 2 decimal places as °Z

5. Calculation

P20 = Ptr – Pw + tr corr. – tm corr. – scale & inst. corr.

Where tr corr. = cPtr (tr-20) – 0.004 R(tr – 20 )
c = coefficient in table 1
R = Reducing sugars, % on sample

tm corr. = f Ptr (tm – 20 )

f = coefficient in table 1

Scale & inst. corr = Qtq – Q20 – Pa – 0.000144 Q20 (tq – 20 )

Table -1

Material of Construction Coefficients

Tube Flask C f
BS BS 0.000490 0.000270
N BS 0.000485 0.000270
St BS 0.000478 0.000270
BS N 0.000490 0.000255
N N 0.000485 0.000255
St N 0.000478 0.000255

BS = Borosilicate glass

N = Normal glass

St = Steel

The Determination of the Polarisation of Raw Sugar by Polarimetry – ICUMSA

Method GS1/2/3/9-1(2011)

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 Document : Standard Test Procedure Date : 01.04.2013
Department/Function: Quality Rev. No. : 00
Doc. No: YCSR/QD/ STP-01 Page 4 of 6
Title : STP- Determination of the Polarization of Raw Sugar

Weigh 26 gms of
Sample

Dissolve and make up to

100 ml

Measure the
temperature of the soln.

Add lead acetate soln.

and wait for 5mts

Filter the solution thro’

watmann 91 filter paper

Rinse the pol tube and

fill with solution

Measure the pol in

polarimeter

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Title : STP- Determination of the Polarization of Raw Sugar

Record temperature of
the solution

Record the polarimeter

reading in air

Record the Quartz plate

reading and its temp.

Record polarimeter
reading for water

Calculate the pol value

as mentioned in page 3

Annexure

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Doc. No: YCSR/QD/ STP-01 Page 6 of 6
Title : STP- Determination of the Polarization of Raw Sugar

Amendment Record:

SNo Current Previous Revision Change initiated Brief description of

Revision the changes made

No. Date No. Date By Through

1. 00 01.04.13 - - - - Initial Issue

2.

3.

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