ESTIMATION OF ASCORBIC ACID

A) STANDARDIZATION

Into an Erlenmeyer flask pipette Titrate with ascorbic acid until

Add the standard ascorbic acid there is a disapearance of pink
into a burette 20 mL of dichlorophenol-
indophenol sol'n colour.

Repeat the titration for atleast 3

Calculate mean and standard times,recording observation.
deviation (ascorbic acid calculate mean and standard
equivalence per 1 mL dye) deviation (ascorbic acid
equivalence per 1 mL dye)
 B) ESTIMATION OF ASCORBIC ACID IN CANNED JUICE

Measure 25mL of the preparation

Into a 500mL beaker pour 2 mL
using measuring cyclinder Make up volume to 50 mL by the
of canned juice and acidify with
tranferring to a 50 mLconical addition of distilled water and
0.2 mL glacial acetic acid,make
flask,followed by addition of 5 % transfer to burette
up volume with distilled water.
meta phosphoric acid.

In mg per mL of canned orange For the dichlorophenol

juice calculate ascorbic acid -indophenol solution titrate
content. atleast three (3) ,5 mL volumes.

Put 100mL of distilled water
an approx. 50g banana in
ziplock bag and crush.
Place test tube with alcohol
and filtrate it in a test tube
holder,let it sit undisturbed for
4 mins.Dip glass rod to spool
out banana's DNA.
C) EXTRACTING DNA FROM BANANAS
Mix 2mL od soap with 0.5g
salt in small ziplock bag +
4mL of distilled water .Mix
gently to avoid creating
foam ; continue until soap and
salt dissolve.
Remove the filter and set aside
then get a test tube of cold
alcohol ,use a pipette or
eyedropper to collect fiiltrate
and add it to alcohol.
Add 10 mL of banana mix for
atleast 5 mins.
Insert a filter in a clean plastic
cup so it doesn't touch the
bottom of cup + pour mixture
from step 3 into filter,after 5
mins,some liquid called filtrate
should have collectted at the
bottom.