Documenti di Didattica
Documenti di Professioni
Documenti di Cultura
C o n t r o l l e d C o p y No. 1
30 July
1997 :•"
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lURPOSE
1COPE
EFERENCES
\A 4 5 0 0 - H + B Electrometric M e t h o d
it Regional M e t h o d W L 120
INITIONS
lOCEDURE
1. G e n e r a l
C o n t r o l of T e s t E q u i p m e n t
3. Calibration of pH Meters
3.1 P r e p a r a t i o n of Buffer S o l u t i o n s
le values in brackets are interpolated from the 20°C and 25°C values given in the British
3.2 Calibrate o n U s e
Calibrate t h e p H m e t e r s a s per A G A L R e g i o n a l M e t h o d W L 120 with p H 7 a n d p H 4
buffer solutions e a c h d a y before u s e . Calibrate t h e p H meter with p H 7 a n d p H 9.2
buffer solutions if it is e x p e c t e d that t h e s a m p l e p H value will b e g r e a t e r t h a n p H 7.
Record the calibration details in t h e p H m e t e r log book.
3.3 C h e c k R e f e r e n c e J u n c t i o n E l e c t r o d e
Measure t h e p H of t h e phosphate buffer solution a n d c o m p a r e w i t h t h e p H of t h e
s a m e buffer solution diluted 1:10 with w a t e r (i.e. diluted with 9 p a r t s of w a t e r ) . T h e
p H of t h e diluted buffer should be 0.15 - 0.25 p H units higher t h a n t h e o r i g i n a l
buffer. Record t h e a p p r o p r i a t e details in t h e p H meter log book.
3.4 C h e c k S l o p e R e s p o n s e of the E l e c t r o d e S y s t e m
T h e slope r e s p o n s e of t h e electrode s y s t e m shall be 95_percent t o 102 p e r c e n t of
t h e theoretical v a l u e (S) for t h e glass electrode at t h e t e m p e r a t u r e of m e a s u r e m e n t ,
i.e.
58.167 m V / p H unit at 2 0 ° C -
59.157 m V / p H unit at 2 5 ° C ^ -
ApH x S
where
mV7 = e l e c t r o d e potential in p H 6.88 buffer s o l u t i o n , in millivolts . . .
mV4 - e l e c t r o d e potential in p H 4 buffer solution, in millivolts
ApH = n o m i n a l p H of t h e buffer p H 6.88 solution minus n o m i n a l p H of t h e p H
4 buffer solution (e.g.. 6.88 - 4.0 = 2.88)
S = t h e o r e t i c a l slope response at t h e t e m p e r a t u r e of m e a s u r e m e n t ( s e e
above)
3.5 C h e c k C o m m e r c i a l pH B u f f e r s
Calibrate t h e p H meter with either previously verified c o m m e r c i a l p H buffers o r
make the p h o s p h a t e buffer solution a n d t h e phthalate buffer s o l u t i o n as s h o w n
above. M e a s u r e t h e p H of t h e n e w commercial pH buffer (before initial u s e ) . T h e
new buffer is a c c e p t a b l e if it is within + 0.02 pH units of t h e notional value. R e c o r d
the appropriate details in t h e p H meter log book.
3.6 E x t e r n a l C a l i b r a t i o n of pH Meters
Not applicable.
Acceptability Criteria
W h e n t h e p H meter d o e s not meet t h e a c c e p t a b l y criteria as s p e c i f i e d below t h e n
the test should be r e p e a t e d . If t h e p H meter does not c o m p l y o n t h e s e c o n d test t h e n
it should be r e g a r d e d a s n o n c o n f o r m i n g equipment a n d treated a s specified i n
section 2.
4.1 R e f e r e n c e J u n c t i o n E l e c t r o d e
T h e pH of t h e d i l u t e d buffer s h o u l d be 0.15 0.25 pH units higher t h a n the o r i g i n a l
buffer.
4.2 S l o p e R e s p o n s e * /
T h e slope r e s p o n s e of t h e e l e c t r o d e system shall be 9 5 percent t o 1 0 2 percent o f
the t h e o r e t i c a l v a l u e (S) for t h e glass electrode at t h e t e m p e r a t u r e o f
measurement.
4.3 C o m m e r c i a l p H B u f f e r s
T h e n e w c o m m e r c i a l buffer is a c c e p t a b l e if it is within + 0.02 p H units of its n o t i o n a l
value. —
5.1 pH P r o b e S t o r a g e S o l u t i o n
W h i l e the p H p r o b e is not in u s e leave it soaking in the p H probe s t o r a g e s o l u t i o n .
W e i g h approx 0.71 g Na2HPC>4 (or approx 0.88g N a H P 0 . 2 H 2 0 ) , approx 0 . 6 8 g
2 4
5.2 C l e a n i n g the p H P r o b e
The pH probe s h o u l d b e cleaned at least every 6 m o n t h s .
Dip the p r o b e in :
1 % pepsin in 0.1 M H C I for 30 m i n s , (to r e m o v e protein materials),
0.1 M E D T A solution f o r 3 0 mins (to r e m o v e inorganic coatings),
a mild d e t e r g e n t s o l u t i o n (or a solvent) for 2 hours (to remove oils a n d g r e a s e s ) ,
then
drain a n d refill t h e reference c h a m b e r with s a t u r a t e d KCI s o l u t i o n a n d a d d s o m e
KCI granules a n d s o a k t h e probe in t h e p H 7 storage solution o v e r n i g h t .
Recalibrate t h e p H p r o b e as specified in section 3.
Record t h e a p p r o p r i a t e details in t h e p H m e t e r log book.
5.3 Maintenance D e t a i l s
Record any c h a n g e s t o t h e p H meter, e . g . battery replacement, n e w p r o b e i n s t a l l e d
etc in t h e p H meter log book.
PONSIBILITY
ATED P R O C E D U R E S ; "
edure for I n s p e c t i o n , M e a s u r i n g , & T e s t E q u i p m e n t
ERENCE DOCUMENTS
applicable.
WCHARTS
e.
10#1 on k:\quality\procedures
kiV^ualityVproceduiNproqlOS 1 .doc