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ChemStation E-Seminar
Page 1
Dec, 2008
ChemStation E-Seminar Agenda
Schedule Short description Agilent
Speaker
OpenLAB Overview
C lib ti Setting
Calibration S tti
Multilevel calibration
Questions
ChemStation E-Seminar
Page 3
Dec, 2008
Q
Quantification
tifi ti - Calibration
C lib ti
Page 4
In this Section, We Will Discuss:
5
Page 5
Building a Calibration Table
4 IIntegrate
4. t t eachh additional
dditi l standard
t d d level
l l andd add
dd to
t the
th
calibration table.
0.747
mAU
100
021
1.0
80
60
Standard
ISTD
2.565
40
5.837
7
20
0
1 2 3 4 5 6 min
3 Levels
ng/μL ng/μL
1. Standard 30.0 ISTD 50.0
2. Standard 50.0 ISTD 50.0
3 Standard 75.0
3. 75 0 ISTD 50.0
50 0
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Signal Details
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Calibration Settings
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Load the Signal(s) for the Low Level Standard and
Integrate
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Create a Calibration Table: Level One
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Calibration Table Overview: Fill in the Dialog
Fill in:
Compound Name
Amount
Check if:
Reference Peak
ISTD
Indicate ISTD #
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Demo Calibration
ChemStation E-Seminar
Page 13
Dec, 2008
Load the Signal - Add Level Two
Add Level 3
and fill in the
amount
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Calibration Table Options
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Compound Details
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Peak Details
By default
default, the largest peak of a compound is the Main peak.
peak
Others are set to Ignore.
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Qualifiers
The ratio of 230 to 254 is 38% The ratio of 230 to 254 is 224%.
224%
for Biphenyl The peak would be rejected
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Edit Calibration Table Options
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Quantification Tools
Calibration
C lib i TaskT k Add New Level Edit Calibration
Edi C lib i
Tool Table Options
Add New Peaks Signal Details
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Save Method
• M
Method
th d Development
D l t changes
h (i.e.
(i calibration
lib ti
table creation / updates) should be saved to the
Current Method (Master Method).
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Information on ChemStation
Documentation can be found on the Agilent
g website
http://www.chem.agilent.com/Scripts/PDS.asp?lPage=61233
• Product Datasheet
• Specification
• Application Notes
• Manuals
ChemStation E-Seminar
Page 25
March,
Dec, 2008
Learning Products – North America Course
Catalog
Users needing to increase productivity in the lab by utilizing both standard and
advanced features available in the Agilent GC/LC ChemStation may want to
attend one of the following courses:
Course Features
For more information concerning course content, dates and locations, please visit:
http://www chem agilent com/en-US/education/en-us/classroomtraining/Pages/Courselisting
http://www.chem.agilent.com/en US/education/en us/classroomtraining/Pages/Courselisting.aspx
aspx
ChemStation E-Seminar
Page 26
Dec, 2008
Learning Products – European Course Catalog
Users needing to increase productivity in the lab by utilizing both standard and
advanced features available in the Agilent GC/LC ChemStation may want to
attend one of the following courses:
¾ H4033A – Agilent HPLC (3D) Method&Run Control, Data Analysis and Reporting (4 days)
Course Features
For more information concerning course content, dates and locations, please visit:
htt //
http://www.chem.agilent.com/en-US/education/pages/homepage.aspx
h il t / US/ d ti / /h
ChemStation E-Seminar
Page 27
Dec, 2008
Who to contact for more information about
OpenLAB and Agilent software products
1. Agilent Account Managers (sales)
2 Agilent Informatics Product Specialists
2.
Eastern US - Marc Mandelbaum – 302-683-7149
Pacific Coast (CA, WA, OR) - Spencer Tse 408-553-3655
Midwest and South - Johnathon McSayles 847-944-6019
Midwest /West/Latin America – Stephen Brown 847-944-6461
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QUESTIONS?
ChemStation E-Seminar
Page 29
Dec, 2008