CLS 262 APPLIED CLINICAL MICROBIOLOGY

FOR DENTAL HYGIENISTS

FALL SEMESTER 2009

Instructor: Dianna Ladesic, MT(ASCP)SM

Office: Bldg. A, Room 171-D, West Charleston Campus

Office Phone 651-5788
Office Hours: Mon /Wed- 9:30-11:30AM, Tues/Thurs- 1:15PM - 1:45PM

Class Time: Monday, 2:00 - 4:50pm

Credit Hours: 1
________________________________________________________________________
Course Description: A laboratory course emphasizing isolation and identification of
pathogenic bacteria, through the use of conventional and commercial methods,
microscopic techniques, and serological tests. Specimen collection and processing of
microbiological samples will be addressed.

Grading Scale: A = 90 - 100%

B = 80 - 89%
C = 70 - 79%
D = 60 - 69%
F = less than 60%

Exams: 17 Lab Reports (10 points

each) = 170 points
11 Lab Quizzes (10 points each) = 110 points
Journal Club Presentation = 100 points
380 points

Attendance: Attendance is mandatory for all laboratory sessions.

There are no makeup labs. If an emergency should arise, the
instructor should be notified in advance if possible. The student
is responsible for all materials covered.

NOTE: Information contained in this syllabus, other than the grading and attendance policies, may be subject to
change with advanced notice, as deemed appropriate by the instructor.
Journal Club Presentation

During the course of the semester, each student will research and present a journal article
pertinent to dental practice. The journal article must have a microbiological emphasis
and be chosen from an appropriate peer reviewed journal. Each student must have their
chosen article approved by the instructor. Each student must also have a set of questions
prepared for their discussion which must be approved by the instructor. The article must
be distributed to your classmates and instructor 7 days prior to the presentation. The
presenting student will be responsible for leading a discussion session on the article,
based on the study questions, during scheduled class time. This is meant to be an
interactive session between you and your classmates. Class participation will be a graded
portion of the exercise.

Course Objectives

Describe and become familiar with a variety of pertinent clinical bacterial isolates.

Identify and/or perform proper collection procedures and transport requirements for clinical
specimens.

Perform and understand principles of microscopy.

Report laboratory data in clear, concise and accurate terms following procedural protocol.

Demonstrate appropriate staining techniques used in the detection of bacteria (including the
Gram stain).

Accurately read and report Gram stain results of microorganisms and host cells.

Properly inoculate suitable media for the cultivation and isolation of clinical bacteria.

Perform biochemical and serologic laboratory procedures used in the identification of bacteria.

Accurately read and record results of identification tests for bacteria and identify the more
commonly seen clinical isolates.

Correlate knowledge if isolated bacteria with specimen normal flora and disease potential.

Perform and read antimicrobial susceptibility testing on appropriate clinical isolates.

Describe and demonstrate the effects of environmental influences (i.e. pH, UV light) on bacterial
growth.
Communicate effectively in written and spoken English.
Demonstrate effective time management skills.

Demonstrate interest in the course material and attend all laboratory sessions.

Project an image of professionalism in appearance and demeanor.

Show respect for self, classmates, and instructors.

Academic Dishonesty

Cheating, plagiarism, and other acts of academic dishonesty are held as serious offenses.
Instructors have the responsibility to report any such incident in writing to the Provost. Serious
penalties may be imposed which, depending on the nature of the incident, could range from loss
of points to permanent expulsion from the class or college.

Classroom Behavior

Instructors have the responsibility to set and maintain standards of classroom behavior
appropriate to the discipline and method of teaching. Students may not engage in any activity
which the instructor deems disruptive or counterproductive to the goals of the class. Beepers,
pagers, and cellular phones can be a nuisance and are not to be brought to the classroom.
Instructors have the right to remove offending students from class. Repetition of the offense
may result in expulsion from the course.

Safety and Security Considerations

Safety and emergency procedures will be discussed during the first class session. Please note the
escape routes posted in the hallways. Ask your instructor where the nearest public telephone is
located. Approved classroom safety procedures are posted in each classroom and are to be
followed.

The CSN Security Office is located in the Main Lobby of the Charleston Campus. Security
Officers provide information, assistance and protection for the students and visitors at CSN. It is
advised that the student make friends and/or family aware of their course schedule in case of an
emergency. Family members may contact the Security Office in the case of an emergency to
contact the student. The phone number for the Security Office is 651-5613.

The Security Officers also patrol the parking areas on a regular basis and are radio dispatched
for emergencies. Escort services are available on request.

Americans with Disabilities Act

If you have a documented disability that may require assistance, you will need to contact the
Disability Resource Center (DRC) for coordination of your academic accommodations. The
phone numbers for each DRC office are: West Charleston 651-5644, Cheyenne 651-4045, and
Henderson 651-3795. For students that would like to perform community service or earn extra
money, stop by the DRC to fill out a job interest card. This office hires students as note takers,
proctors, scribes and lab assistants as needed.

DATE LAB TOPIC

August 31 Introduction, Lab Safety

September 7 LABOR DAY HOLIDAY

September 14 Exercise 1 - Microscopy

September 21 Quiz #1
Exercise 2 - Aseptic Transfer Techniques

September 28 Quiz #2
Exercise 3 - Smear Preparation
Exercise 4 - Gram Stain
Exercise 5 - Capsule Stain (Negative Stain)
Lab Report for Ex. 1 & 2 due

October 5 Quiz #3
Exercise 6 - Pure Culture Isolation Skills
Lab Reports for Ex. 3, 4, & 5 due

October 12 Quiz #4
Exercise 7 - Determination of Oxygen Requirements
Exercise 8 – Temperature and Bacterial growth
Lab Report for Ex. 6 due

October 19 Quiz #5
Exercise 9 – pH and Microbial Growth
Journal Club presentation
Lab Reports for Ex. 7 & 8 due

October 26 Quiz #6
Exercise 10 – Synergism
Exercise 11 - Commensalism
Journal Club presentation
Lab Report for Ex. 9

November 2 Quiz #7
Exercise 12- Antagonism
Journal Club presentation
 Lab Report for Ex. 10 & 11 due

November 9 No Lab
DATE LAB TOPIC

November 16 Quiz #8
Exercise 13 –Effects of UV Radiation
Journal Club presentation
Lab Reports for Ex. 12 due

November 23 Quiz #9
Exercise 14 –Chemical Control of Microorganisms
Exercise 15 –Antibiotic Sensitivity Testing
Journal Club presentation
Lab Report for Ex. 13 due

November 30 Quiz #10

Exercise 16 –Triple Sugar Iron - Fermentation
Exercise 17 – Snyder Test for Dental Caries
Journal Club presentation
Lab Report for Ex. 14 & 15 due

December 7 Quiz #11

Journal Club presentation
Lab Reports for Ex. 15 & 16 due