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ELISA kits and reagents

for Immuno-Oncology

www.abcam.com/IO-ELISA
ELISA kits and reagents for Immuno-oncology
Our range of ELISA kits include our 90-min, single-wash SimpleStep ELISA® kits and fluorescent
CatchPoint® SimpleStep ELISA® (SSE) that contain recombinant monoclonal antibodies for
specific and sensitive results.

Our matched antibody pair kits for ELISA are made from recombinant monoclonal
antibodies for consistent, specific, and sensitive. They include a titrated capture and
biotinylated detector antibody pair and a calibrated protein standard.

B7-CD28 ligand-receptor family ELISA kits


Target name Species abID SSE Sensitivity Range Assay Validated sample types
(pg/mL) (pg/mL) time
CS S HP EP CP U Sv M CE TE CL TL TH CSF CM

B7-H4 Hu ab233633 0.03 0.023 - 1.5 1h 30m ●

CD28 Hu ab119514 0.18 0.78 - 50 3h 30m ● ●


CD80 (B7-1) Hu ab171342 0.01 0.062 - 4 3h 30m ● ●
CD80 (B7-1) Ms ab119578 0.01 0.062 - 4 3h 30m ● ●
CD276 (B7-H3) Hu ab221831 0.178 0.578 - 37 1h 30m ● ● ● ● ● ●
CTLA-4 Ms ab113346 <0.005 0.005 - 1.2 4h 45m ● ● ● ● ●
PD-1 Ms ab210971 0.021 0.125 - 8 1h 30m ● ● ●
PD-1H (VISTA) (B7-H5) Ms ab231933 0.044 0.391 - 25 1h 30m ● ● ● ● ● ● ● ● ●
PD-L1 (B7-H1) Hu ab214565 0.003 0.022 - 1.4 1h 30m ● ● ● ● ● ● ● ●
PD-L1 (B7-H1) Hu ab229414 0.002 0.003 - 2.8 1h 30m ● ● ● ● ● ● ● ●
PD-L2 (B7-DC) Hu ab231928 0.003 0.015 - 1 1h 30m ● ● ● ● ● ● ● ●

B7-CD28 ligand-receptor family matched antibody pair kits


Target name Species abID Range (pg/mL)

CD276 (B7-H3) Hu ab222281 0.250 - 16


PD-1 Ms ab217609 0.250 - 16

Chemokines and chemokine ligands ELISA kits


Target name Species abID SSE Sensitivity Range Assay Validated sample types
(pg/mL) (pg/mL) time
CS S HP EP CP U Sv M CE TE CL TL TH CSF CM

CXCL5 Hu ab212163 0.002 0.015 - 1 1h 30m ● ● ● ● ●


CXCL5 Ms ab100719 <0.035 0.031 - 2 4h 45m ● ● ● ● ●
CXCL5 Rt ab100774 <0.02 0.025 - 6 4h 45m ● ● ● ● ●
CXCL5 Rt ab100775 <0.015 0.008 - 6 4h 45m ● ●
IL-8 Hu ab214030 <0.002 0.004 - 0.25 1h 30m ● ● ● ● ●
IL-8 Hu ab229402 <0.001 0.0005 - 2 1h 30m ● ● ● ● ●
IL-8 Hu ab46032 <0.025 0.062 - 2 1h 40m ● ● ● ● ●
IL-8 Hu ab100575 <0.001 0.001 - 0.6 4h 45m ● ● ● ● ●
IL-8 Hu ab100576 <0.001 0.001 - 0.6 4h 45m ● ●
RANTES (CCL5) Hu ab174446 0.002 0.003 - 0.2 1h 30m ● ● ● ● ● ●
RANTES (CCL5) Hu ab100633 <0.003 0.003 - 2 4h 45m ● ● ● ● ●
RANTES (CCL5) Ms ab215537 0.005 0.008 - 0.5 1h 30m ● ●
RANTES (CCL5) Ms ab100739 <0.001 0.0008 - 0.2 4h 45m ● ● ● ● ●
RANTES (CCL5) Rt ab213907 <0.001 0.016 - 1 3h 30m ● ● ● ● ●

All ELISA in the list are coated ELISA kits, and are available as 1x96 tests. Overall, listed ELISA kits have intra- and inter-assay precision lower than 10% (CV).

Validated sample types: CS=Cell culture supernatant, S=serum, HP=heparin plasma, EP=EDTA plasma, CP=citrate plasma , U=urine, Sv=saliva, M= milk , CE=cell
culture extract, TE=tissue extract, CL=cell lysate, TL=tissue lysate, TH=tissue homogenate, CSF=Cerebrospinal fluid and CM=cell culture media

= colorimetric SimpleStep ELISA®, = fluorescent Catchpoint® SimpleStep ELISA®, SQ: semi-quantitative


Chemokines and chemokine ligands matched antibody pair kits
Target name Species abID Range (pg/mL)

CXCL5 Hu ab215074 0.004 - 0.25


IL-8 Hu ab215402 0.002 - 0.125
RANTES (CCL5) Ms ab213736 0.008 - 0.5

Cytokines and cytokine receptors ELISA kits


Target name Species abID SSE Sensitivity Range Assay Validated sample types
(pg/mL) (pg/mL) time
CS S HP EP CP U Sv M CE TE CL TL TH CSF CM

IFNg Hu ab174443 0.47 0.468 - 30 1h 30m ● ● ● ● ●


IFNg Hu ab229415 0.025 0.060 - 30 1h 30m ● ● ● ● ●
IFNg Hu ab46025 <0.005 0.012 - 0.4 2h 40m ● ● ● ● ●
IFNg Hu ab46048 <0.0007 0.0007 - 0.025 3h 00m ● ● ● ● ●
IFNg Hu ab100537 <0.015 0.02 - 15 4h 45m ● ● ● ●
IFNg Ms ab100689 <0.005 0.003 - 2 4h 45m ● ● ● ● ●
IFNg Ms ab100690 <0.01 0.003 - 2 4h 45m ● ● ● ● ● ●
IFNg Ms ab46081 <0.015 0.031 - 1 3h 45m ● ●
IFNg Rt ab46107 <0.01 0.031 - 1 3h 45m ● ● ● ●
IL-1b Hu ab214025 0.005 0.014 - 0.9 1h 30m ● ● ● ● ●
IL-1b Hu ab100562 <0.0003 0.048 - 0.1 4h 45m ● ● ● ●
IL-1b Hu ab46052 0.006 0.016 - 0.5 3h 45m ● ● ● ● ●
IL-1b Ms ab197742 0.001 0.001 - 0.1 1h 30m ● ● ● ● ● ● ● ●
IL-1b Ms ab100704 <0.005 0.003 - 2 4h 45m ● ● ● ●
IL-1b Ms ab100705 <0.005 0.003 - 2 4h 45m ● ●
IL2-R (CD122) Hu ab46036 0.032 0.069 - 2.22 3h 45m ● ● ● ● ●
IL-4 Hu ab215089 0.001 0.006 - 0.4 1h 30m ● ● ● ● ●
IL-4 Hu ab46063 0.0003 0.0003 - 0.1 3h 15m ● ● ● ● ●
IL-4 Hu ab100570 <0.005 0.003 - 0.2 4h 45m ● ● ● ● ●
IL-4 Ms ab221833 0.006 0.018 - 1.2 1h 30m ● ● ● ●
IL-4 Ms ab100710 <0.001 0.0009 - 0.2 4h 45m ● ●
IL-4 Rt ab100770 <0.001 0.0007 - 0.16 4h 45m ● ● ● ● ●
IL-4 Rt ab100771 <0.001 0.0007 - 0.16 4h 45m ● ●
IL-4 Rt ab46073 <0.015 0.031 - 1 4h 45m ● ● ● ●
IL-6 Hu ab178013 0.0016 0.008 - 0.5 1h 30m ● ● ● ● ●
IL-6 Hu ab46042 <0.0008 0.001 - 0.05 3h 45m ● ● ● ● ●
IL-6 Hu ab46027 <0.002 0.006 - 0.2 1h 45m ● ● ● ● ●
IL-6 Hu ab100572 <0.003 0.001 - 1 4h 45m ● ● ● ● ●
IL-6 Ms ab222503 0.011 0.015 - 1 1h 30m ● ● ● ● ●
IL-6 Ms ab100712 <0.002 0.0008 - 0.6 4h 45m ● ● ● ● ●
IL-6 Ms ab100713 <0.002 0.0008 - 0.6 4h 45m ● ●
IL-10 Hu ab185986 0.006 0.006 - 5 1h 30m ● ● ● ● ●
IL-10 Hu ab229436 0.005 0.006 - 45 1h 30m ● ● ● ● ●
IL-10 Hu ab46059 <0.001 0.001 - 0.05 3h 15m ● ● ● ● ●
IL-10 Hu ab100549 <0.001 0.002 - 0.15 4h 45m ● ● ● ● ●
IL-10 Hu ab46034 <0.005 0.012 - 0.4 2h 45m ● ● ● ● ●
IL-10 Ms ab46103 <0.02 0.031 - 1 3h 45m ● ●
IL-10 Ms ab100697 <0.045 0.078 - 5 4h 45m ● ● ● ● ●
IL-10 Ms ab108870 0.03 0.031 - 2 5h 00m ● ● ● ● ● ●
IL-10 Rt ab214566 0.005 0.031 - 2 1h 30m ● ● ●
IL-10 Rt ab100764 <0.01 0.008 - 6 4h 45m ● ● ● ● ●
IL-10 Rt ab100765 <10 0.082 - 6 4h 45m ● ●
IL-12-p70 Hu ab223592 0.014 0.031 - 2 1h 30m ● ● ● ● ●
IL-12-p70 Hu ab213791 <0.002 0.008 - 0.5 3h 30m ● ●
IL-12-p70 Hu ab100552 <0.001 0.0008 - 0.6 4h 45m ● ● ● ●

All ELISA in the list are coated ELISA kits, and are available as 1x96 tests. Overall, listed ELISA kits have intra- and inter-assay precision lower than 10% (CV).

Validated sample types: CS=Cell culture supernatant, S=serum, HP=heparin plasma, EP=EDTA plasma, CP=citrate plasma , U=urine, Sv=saliva, M= milk , CE=cell
culture extract, TE=tissue extract, CL=cell lysate, TL=tissue lysate, TH=tissue homogenate, CSF=Cerebrospinal fluid and CM=cell culture media

= colorimetric SimpleStep ELISA®, = fluorescent Catchpoint® SimpleStep ELISA®, SQ: semi-quantitative


Cytokines and cytokine receptors ELISA kits (continued)
Target name Species abID SSE Sensitivity Range Assay Validated sample types
(pg/mL) (pg/mL) time
CS S HP EP CP U Sv M CE TE CL TL TH CSF CM

IL-12/IL-23 p40 Hu ab220656 0.006 0.031 - 2 1h 30m ● ● ● ● ●


IL-13 Hu ab178014 0.039 0.078 - 5 1h 30m ● ● ● ● ●
IL-13 Hu ab46038 <0.001 0.003 - 0.1 2h 10m ● ● ● ● ●
IL-13 Hu ab100553 <0.00015 0.0001 - 0.04 4h 45m ● ● ● ● ●
IL-13 Ms ab219634 0.001 0.002 - 0.125 1h 30m ● ● ● ● ●
IL-13 Ms ab100700 <0.006 0.008 - 2 4h 45m ● ● ● ● ●
IL-13 Rt ab100766 <0.02 0.027 - 20 4h 45m ● ●
IL-21 Hu ab119542 0.02 0.078 - 5 4h 10m ● ●
IL-21 Ms ab100707 <0.006 0.008 - 2 4h 45m ● ● ● ●
IL-21 Ms ab171180 <0.01 0.062 - 4 3h 45m ● ●
IL-23 Hu ab221837 0.016 0.039 - 2.5 1h 30m ● ● ● ● ●
IL-23 Hu ab64708 <0.02 0.156 - 5 2h 45m ● ● ● ● ●
MCP1 (CCL2) Hu ab179886 0.001 0.005 - 0.3 1h 30m ● ● ● ● ● ●
MCP1 (CCL2) Hu ab100586 <0.002 0.002 - 0.5 4h 45m ● ● ● ● ●
MCP1 (CCL2) Ms ab208979 0.0005 0.001 - 0.1 1h 30m ● ● ● ● ●
MCP1 (CCL2) Ms ab100721 <0.003 0.0027 - 2 4h 45m ● ● ● ● ●
MCP1 (CCL2) Ms ab100722 <0.003 0.0027 - 2 4h 45m ● ●
MCP1 (CCL2) Rt ab219045 0.006 0.013 - 0.815 1h 30m ● ● ● ● ●
MCP1 (CCL2) Rt ab100777 <0.015 0.025 - 18 4h 45m ● ● ● ● ●
MCP1 (CCL2) Rt ab100778 <0.015 0.025 - 18 4h 45m ● ●
TSLP Hu ab192149 0.003 0.016 - 1 1h 30m ● ●
TSLP Hu ab155444 <0.003 0.003 - 0.8 4h 45m ● ● ● ● ●
TSLP Ms ab155461 <0.001 0.001 - 0.2 4h 45m ● ● ● ● ●

Cytokines and cytokine receptors matched antibody pair kits


Target name Species abID Range (pg/mL)

IL-1b Hu ab217608 0.031 - 2


IL-1b Ms ab210895 0.007 - 0.5
IL-4 Hu ab218800 0.004 - 0.25
IL-4 Ms ab222274 0.001 - 0.125
IL-6 Ms ab213749 0.015 - 0.1
IL-10 Ms ab214473 0.008 - 0.5
IL-10 Rt ab218796 0.031 - 2
IL-12/IL-23 p40 Hu ab222270 0.016 - 1
IL-13 Hu ab224648 0.016 - 1
IL-13 Ms ab221432 0.001 - 0.125
IL-23 Hu ab222278 0.016 - 1
MCP-1 (CCL2) Hu ab214476 0.004 - 0.25
MCP-1 (CCL2) Ms ab210894 0.003 - 0.2
MCP-1 (CCL2) Rt ab221434 0.004 - 0.25

All ELISA in the list are coated ELISA kits, and are available as 1x96 tests. Overall, listed ELISA kits have intra- and inter-assay precision lower than 10% (CV).

Validated sample types: CS=Cell culture supernatant, S=serum, HP=heparin plasma, EP=EDTA plasma, CP=citrate plasma , U=urine, Sv=saliva, M= milk , CE=cell
culture extract, TE=tissue extract, CL=cell lysate, TL=tissue lysate, TH=tissue homogenate, CSF=Cerebrospinal fluid and CM=cell culture media

= colorimetric SimpleStep ELISA®, = fluorescent Catchpoint® SimpleStep ELISA®, SQ: semi-quantitative


Immunoglobulin superfamily ELISA kits
Target name Species abID SSE Sensitivity Range Assay Validated sample types
(pg/mL) (pg/mL) time
CS S HP EP CP U Sv M CE TE CL TL TH CSF CM

CD4 Hu ab234569 0.013 0.47 - 30 1h 30m ●


CD86 Hu ab45921 0.6 U/mL 1.187 - 38 U/mL 2h 40m ● ● ● ● ●
CD86 Rt ab171388 <0.01 0.062 - 4 3h 30m ● ●
LAG-3 Hu ab193707 0.045 0.045 - 12 4h 45m ● ● ● ● ●

MHC class I ELISA kits


Target name Species abID SSE Sensitivity Range Assay Validated sample types
(pg/mL) (pg/mL) time
CS S HP EP CP U Sv M CE TE CL TL TH CSF CM

B2M Hu ab181423 0.031 0.031 - 2 1h 30m ● ● ● ● ● ●


B2M Hu ab99977 <0.006 0.015 - 1 4h 45m ● ● ● ● ●
B2M Hu ab108885 0.19 1 - 20 4h 00m ● ● ● ● ● ● ● ● ●

TIM family ELISA kits


Target name Species abID SSE Sensitivity Range Assay Validated sample types
(pg/mL) (pg/mL) time
CS S HP EP CP U Sv M CE TE CL TL TH CSF CM

GAL9 Hu ab213786 <0.01 0.094 - 6 3h 30m ● ● ● ● ● ●


TIM-1 (KIM1) Hu ab235081 0.001 0.008 - 0.5 2h 00m ●
TIM-1 (KIM1) Ms ab213477 0.001 0.006 - 0.4 1h 30m ● ● ● ● ● ● ●
TIM-1 (KIM1) Ms ab119596 <0.002 0.031 - 2 3h 40m ● ● ● ● ● ●
TIM-1 (KIM1) Rt ab119597 <0.002 0.031 - 2 3h 40m ● ● ● ● ● ●
TIM-3 Hu ab231932 0.015 0.078 - 5 1h 30m ● ● ● ● ● ● ●

TIM family matched antibody pair kits


Target name Species abID Range (pg/mL)

TIM-1 (KIM-1) Ms ab216792 0.008 - 0.5

TNF and TNF R superfamilies ELISA kits


Target name Species abID SSE Sensitivity Range Assay Validated sample types
(pg/mL) (pg/mL) time
CS S HP EP CP U Sv M CE TE CL TL TH CSF CM

APRIL Hu ab119505 0.4 0.78 - 50 3h 10m ● ● ● ● ●


BAFF Hu ab188391 0.013 0.08 - 5 1h 30m ● ● ● ●
BAFF Ms ab216948 0.007 0.031 - 2 1h 30m ● ● ● ●
BCMA (TNFRSF17) Hu ab213840 <0.01 0.031 - 2 3h 30m ● ● ● ● ● ●
BCMA (TNFRSF17) Ms ab213893 <0.01 0.031 - 2 3h 30m ● ● ● ● ● ●
CD137 Hu ab229892 0.008 0.047 - 3 1h 30m ● ● ● ● ● ●
CD137 Ms ab205577 0.014 0.078 - 5 1h 30m ● ● ● ● ● ● ●
CD154 (CD40L) Hu ab196268 0.006 0.015 - 1 1h 30m ● ● ● ● ●
CD154 (CD40L) Hu ab99991 <0.006 0.008 - 6 4h 45m ● ● ● ●
CD154 (CD40L) Ms ab119517 0.14 0.31 - 20 3h 30m ● ●
CD27 (TNFRSF7) Ms ab233619 0.011 0.031 - 2 1h 30m ● ● ● ● ●
CD27 (TNFRSF7) Ms ab213895 <0.01 0.062 - 4 3h 30m ● ●
CD40 Hu ab210970 0.005 0.031 - 2 1h 30m ● ● ● ● ● ● ● ● ● ●

All ELISA in the list are coated ELISA kits, and are available as 1x96 tests. Overall, listed ELISA kits have intra- and inter-assay precision lower than 10% (CV).

Validated sample types: CS=Cell culture supernatant, S=serum, HP=heparin plasma, EP=EDTA plasma, CP=citrate plasma , U=urine, Sv=saliva, M= milk , CE=cell
culture extract, TE=tissue extract, CL=cell lysate, TL=tissue lysate, TH=tissue homogenate, CSF=Cerebrospinal fluid and CM=cell culture media

= colorimetric SimpleStep ELISA®, = fluorescent Catchpoint® SimpleStep ELISA®, SQ: semi-quantitative


TNF and TNF R superfamilies ELISA kits (continued)
Target name Species abID SSE Sensitivity Range Assay Validated sample types
(pg/mL) (pg/mL) time
CS S HP EP CP U Sv M CE TE CL TL TH CSF CM

CD40 Hu ab99990 <0.05 0.055 - 40 4h 45m ● ● ● ● ●


CD40 Hu ab119516 0.001 0.007 - 0.5 3h 30m ● ● ● ●
CD40 Ms ab206307 0.01 0.062 - 4 1h 30m ● ● ● ● ● ● ● ●
CD40 Ms ab100674 <0.003 0.002 - 1.5 4h 45m ● ● ● ● ●
CD70 Ms ab213854 <0.010 0.031 - 2 3h 30m ● ● ● ● ● ●
GITR (TNFRSF18) Hu ab100527 0.015 0.014 - 10 4h 45m ● ● ● ● ●
GITR (TNFRSF18) Hu ab100528 0.015 0.014 - 10 4h 45m ● ●
GITRL Hu ab113323 <0.025 0.041 - 10 4h 45m ● ● ● ● ●
HVEM (TNFRSF14) Hu ab216950 0.009 0.031 - 2 1h 30m ● ● ● ● ● ● ●
HVEM (TNFRSF14) Hu ab113335 <0.03 0.033 - 8 4h 45m ● ● ● ● ●
HVEM (TNFRSF14) Ms ab213892 <0.001 0.016 - 1 3h 30m ● ● ● ● ● ●
OX40 (CD134) Hu ab231926 0.023 0.047 - 3 1h 30m ● ● ● ● ● ● ●
OX40 (CD134) Hu ab119510 0.002 0.078 - 5 3h 10m ● ●
OX40 (CD134) Ms ab213894 <0.010 0.031 - 2 3h 30m ● ● ● ● ● ●
OX40L (TNFSF4) Hu ab213842 <0.010 0.156 - 10 3h 30m ● ● ● ● ● ●
OX40L (TNFSF4) Ms ab193729 0.03 0.03 - 8 4h 45m ● ● ● ● ●
TNF alpha Hu ab181421 0.014 0.031 - 2 1h 30m ● ● ● ● ●
TNF alpha Hu ab229399 0.002 0.004 - 8 1h 30m ● ● ● ● ●
TNF alpha Hu ab46087 <0.010 0.025 - 0.8 3h 40m ● ● ● ● ●
TNF alpha Hu ab100654 <0.030 0.024 - 6 4h 45m ● ● ● ● ●
TNF alpha Hu ab108908 0.016 0.016 - 1 5h 00m ● ● ● ● ●
TNF alpha Ms ab208348 <0.009 0.047 - 3 1h 30m ● ● ● ● ●
TNF alpha Ms ab229393 0.008 0.011 - 6 1h 30m ● ● ● ● ●
TNF alpha Ms ab100747 <0.06 0.094 - 6 4h 45m ● ● ● ●
TNF alpha Ms ab46105 <0.025 0.031 - 1 3h 45m ● ●
TNF alpha Rt ab100785 <0.025 0.082 - 20 4h 45m ● ●
TNF alpha Rt ab46070 <0.015 0.031 - 1 3h 45m ● ●
TNF alpha Rt ab100784 <0.025 0.082 - 20 4h 45m ● ● ● ● ●
TNFRII Hu ab100643 <0.005 0.003 - 2 4h 45m ● ● ● ● ●
TNFRII Ms ab202412 0.001 0.006 - 0.4 1h 30m ● ● ● ● ●
TWEAK Ms ab113320 <0.0003 0.0003 - 0.2 4h 45m ● ● ● ● ●
TWEAK Rt ab213915 <0.01 0.015 - 1 3h 30m ● ● ● ● ● ●

TNF and TNF R superfamilies matched antibody pair kits


Target name Species abID Range (pg/mL)

CD137 Ms ab216788 0.031 - 2


CD40 Hu ab216055 0.016 - 1
CD40 Ms ab213469 0.016 - 1
HVEM (TNFRSF14) Hu ab220130 0.008 - 0.5
TNF alpha Ms ab212073 0.008 - 0.5
TNF RII Ms ab214482 0.008 - 0.5

All ELISA in the list are coated ELISA kits, and are available as 1x96 tests. Overall, listed ELISA kits have intra- and inter-assay precision lower than 10% (CV).

Validated sample types: CS=Cell culture supernatant, S=serum, HP=heparin plasma, EP=EDTA plasma, CP=citrate plasma , U=urine, Sv=saliva, M= milk , CE=cell
culture extract, TE=tissue extract, CL=cell lysate, TL=tissue lysate, TH=tissue homogenate, CSF=Cerebrospinal fluid and CM=cell culture media

= colorimetric SimpleStep ELISA®, = fluorescent Catchpoint® SimpleStep ELISA®, SQ: semi-quantitative


VEGF and receptor ELISA kits
Target name Species abID SSE Sensitivity Range Assay Validated sample types
(pg/mL) (pg/mL) time
CS S HP EP CP U Sv M CE TE CL TL TH CSF CM

VGEF Hu ab222510 0.003 0.012 - 0.8 1h 30m ● ● ● ● ● ● ● ●


VGEF Hu ab100662 <0.01 0.008 - 6 4h 45m ● ● ● ● ●
VGEF Hu ab100663 <0.01 0.008 - 6 4h 45m ● ●
VGEF Hu ab119566 0.008 0.016 - 1 4h 30m ● ● ● ● ●
VGEF Ms ab100751 <0.002 0.004 - 1 4h 45m ● ● ● ● ●
VGEF Ms ab209882 0.0003 0.006 - 0.37 1h 30m ● ● ●
VGEF Ms ab100752 <0.002 0.004 - 1 4h 45m ● ●
VGEF Ms ab119565 0.02 0.062 - 4 4h 30m ● ● ●
VGEF Rt ab100786 0.002 0.0008 - 0.2 4h 45m ● ● ● ● ●
VGEF Rt ab100787 0.002 0.0008 - 0.2 4h 45m ● ●
VGEF R2 Hu ab213476 0.01 0.117 - 7.5 1h 30m ● ● ● ● ●

VEGF and receptor matched antibody pair kits


Target name Species abID Range (pg/mL)

VEGF Ms ab220118 0.008 - 0.5


VEGF R2 Hu ab221427 0.062 - 4

Other protein and enzyme ELISA kits


Target name Species abID SSE Sensitivity Range Assay Validated sample types
(pg/mL) (pg/mL) time
CS S HP EP CP U Sv M CE TE CL TL TH CSF CM

Arginase 1 Hu ab230930 0.017 0.187 - 12 1h 30m ●


Arginase 1 Rt ab231923 0.022 0.156 - 10 1h 30m ●
CD26 Hu ab119513 0.007 0.015 - 0.5 4h 15m ● ●
CD26 Ms ab155455 <0.45 0.41 - 100 4h 45m ● ● ● ● ●
CD73 Hu ab213761 <0.01 0.031 - 2 3h 30m ● ● ● ● ● ●
Granzyme B Hu ab235635 0.106 0.156 - 10 1h 30m ● ● ● ●
Granzyme B Hu ab46142 <0.02 0.031 - 1 3h 45m ● ● ● ● ●
L Selectin Hu ab45917 <1 1.6 - 60 1h 45m ● ● ● ● ●
L Selectin Ms ab199085 <0.001 0.015 - 1 1h 30m ● ● ● ●
L Selectin Ms ab155448 <0.035 0.041 - 10 4h 45m ● ● ● ● ●
L Selectin Rt ab100776 <0.006 0.008 - 6 4h 45m ● ● ● ● ●
Perforin Hu ab46068 <0.04 0.062 - 2 2h 50m ●
STAT3 Hu ab176655 15000 SQ 1h 30m
STAT3 (pY705) Hu ab176654 30000 SQ 1h 30m ●
STAT3 (pY705) Hu, Ms, ab126458 SQ SQ 5h 00m ●
Rt
STAT3 (pY705) + total Hu ab176666 SQ SQ 1h 30m ●
STAT3 (pY705) + total Hu, Ms, ab126459 SQ SQ 5h 00m ●
Rt

Other protein and enzyme matched antibody pair kits


Target name Species abID Range (pg/mL)

L-selectin (CD62L) Ms ab216050 0.031 - 2

All ELISA in the list are coated ELISA kits, and are available as 1x96 tests. Overall, listed ELISA kits have intra- and inter-assay precision lower than 10% (CV).

Validated sample types: CS=Cell culture supernatant, S=serum, HP=heparin plasma, EP=EDTA plasma, CP=citrate plasma , U=urine, Sv=saliva, M= milk , CE=cell
culture extract, TE=tissue extract, CL=cell lysate, TL=tissue lysate, TH=tissue homogenate, CSF=Cerebrospinal fluid and CM=cell culture media

= colorimetric SimpleStep ELISA®, = fluorescent Catchpoint® SimpleStep ELISA®, SQ: semi-quantitative


Not finding what you are looking for?
Our custom services can help designing and producing matched antibody-pairs and ELISA kits for your
target(s) of interest.

For enquiries, please contact custom@abcam.com

Reagents for assay development


With a continuously-expanding catalog of stable, sensitive and specific recombinant antibodies and pairs
that are provided in a format ready for development, we support innovative platform and instrument
providers in expanding their assay offerings, at speed. Our recombinant antibodies are fully characterized
and supplied with a full data package, helping to reduce assay development costs and time.

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