Maranan, Yvonne R.

October 12, 2018

HUB 44

Experiment No. 5: Reactions of Carbohydrates

SCHEMATIC DIAGRAM
Procedures
A. Identification of Unkonwn Carbohydate Samples

Transfer 1.00 mL of
Add 1.00 mL of
the known
Molisch reagent then Observe for any
carbohydrate samples
1.00 mL of change and record
and the two unknown
concentrated 𝐻2 𝑆𝑂4 to obeservation.
samples on separate
each sample.
labelled test tubes.

Using fresh batch of samples each time, conduct the remaining tests.
a. Iodine Test: add 1.00 mL of iodine reagent to each sample.
b. Benedict's Test: add 1.00 mL of Benedict reagent then heat using water bath.
c. Barfoed's Test: add 1.00 mL of Barfoed's reagent then heat using water bath.
d. Seliwanoff's Test: add 1.00 mL of Saliwanoff reagent then heat using water bath.
e. 2,4-DNP Test: add 1.00 mL of 2,4-DNP and heat using water bath.

Determine the identity of the unknown samples by comparing it to the

known carbohydrate samples.
B. Hydrolysis of Starch

Add 5.00 mL of Cover with an

Transfer 50.0 mL of
concentrated aluminum foil and
5% starch solution
sulfuric acid or heat to boiling
ina 100-mL beaker.
hydrochloric acid. using water bath.

Add1.00 mL of
Transfer two 1.00
iodine reagent to
mL volume of the
one tube and 1.00 Observe reaction.
sample in a test
mL of Benedict's
tube.
reagent to the other.

Transfer two 1.00 mL volume of the sampe every 5

minute interval and test with iodine and Benedict's
Continuue heating
reagent as above until formation of blue-black
sample.
complex in iodine stops and formation of brick red
color in Benedict's reagent ensues.