GTB 204/3 TEKNIK BIOLOGI MOLEKUL

Final exam (2002/03)

Part A
Each question 5 minutes. Answer only six questions.

1. What are the properties of IPTG and X gal and its use in experiment?

2. What are the properties and advantages of eukaryotes expression vector?

3. What are the properties and use of M13 filamentous phage?

4. List down the comparison between Northern blot and Microarray.

5. Huraikan struktur sekunder protein.

6. What is inteference RNA-Irna

7. With the aid of diagram, explain cis acting sequence and trans acting
protein

8. What is the 4 main reason of DNA sequencing?

Part B
Each question 15 minutes. Answer only 2 questions

1. You are going to clone human growth hormone. Design an experiment till
you get the protein ( you are given some key word related to the
experiment- Cloning simulation project)

2. Explain the organization of lac operon and trp operon, and with diagram,
state out their difference regarding regulation.

3. A molecular biologist wants to clone some biological factor regarding

blood clotting. He or she only knows about the small sequence –
ACTGACGCGC. Design steps in getting the whole sequence

Part C
Each question 15 minutes. Answer only 2 questions

1. a) What is codon bias? What are the impacts of codon bias in cloning and
gene expression?

b) Briefly describe assembly PCR and its application

2. a) Design experiment SDS-PAGE and WESTERN blot to separate protein

that found in membran bacteria X.
 b) What are proteomics?

c) With aid of flow chart, list out the steps in proteomics

OSPE (2002/03)

1. Station 1 – you will be given a whole sequence of a gene and ask to

calculate the amino acid molecular weight. What’s the program that do
multiple alignment, define ORF and and list what’s the program that
search for ORF.

2. Station 2 – you are given one SDS page chamber and ask you to label 4
parts and the function of them. Then ask you what the technique that
uses this apparatus is. List out 4 composition of SDS-PAGE gel.

3. Station 3 – you be given one SDS-PAGE gel and ask you what is the stain
that being use and what is the approximate molecular weight of certain
band. What is the component of sample buffer of SDS-PAGE? Way to
destain it?

4. Station 4- given the picture of DNA sequencing and ask you base on the
picture to write down the sequence of that gene from 5’ to 3’. Then ask
you the component of DNA sequencing and then how many primers to
use.
5. Station 5 given a chart about PCR, ask u to write down the 3 steps and the
temperature of the steps and calculate the annealing temperature of
primer and also write down the component of PCR.

6. Station 6 – given you the result of western blot ask u the technique and
then ask you about the membrane, the application of western blot. Stain
of western blot.
7. Station 7- given the agarose gel result, ask the how many base pair of the
result, then ask why got 2 band which is due to contamination, then ask
what is the gel being use, stain of agarose gel

8. Station 8- give you the picture of blue white phenotype, ask you the
function of x gal. the significance of blue colony, 2 other methods of
screening, count the blue colony,

9. Station 9 – given a picture of vector, ask to list the part of certain function
out, then what is the function of lac I gene.

10.Station 10- give you the picture of restriction enzymes out then ask to
calculate how many fragments and then the size and then what is the
enzyme for ligation and also 2 methods of transformation and then. What
is the software for restriction enzyme?