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Talavera MD
EMBRYOLOGY
Embryology
1. Prophase
2. Prometaphase
3. Metaphase
4. Anaphase
5. Telophase
Mitosis - Prophase
■ chromosomes begin
to coil, contract and
condense
becoming shorter
and thicker
■ each consists of 2
chromatids, which
are joined at the
centromere
Mitosis - Prometaphase
■ chromatids become
distinguishable
Mitosis - Metaphase
■ chromosomes line
up in the equatorial
plane
■ each attached by
mitotic spindles or
microtubules
Mitosis - Anaphase
■ centromeres of
each chromosome
divide
■ migration of the
chromatids to
opposite poles of
the spindle
Mitosis - Telophase
■ chromosomes
uncoil and lengthen
■ nuclear envelope
reforms
■ division of the
cytoplasm
Mitosis
■ Pairing or
synapsis of
homologous
chromosome
(bivalents)
■ 1st characteristic
feature of meiosis
■ each consists of 4
chromatids
st
Meiosis – 1 Division
■ Crossover
■ interchange of
chromatid segments
between bivalents
■ chiasm formation
■ 2nd characteristic
feature of meiosis
■ blocks of genes are
exchanged
st
Meiosis – 1 Division
■ Anaphase
■ each daughter cell
contains 1 member
of the chromosome
pair
nd
Meiosis – 2 Division
• no
DNA synthesis
occurs prior to this
stage
• the 23
double-stranded
chromosomes divide at
the centromere and
each receives 23
chromatids
Meiosis – Result
■ 1 primary oocyte
gives rise to 4
daughter cells = 1
mature oocyte + 3
polar bodies
■ 22 + 1X
chromosome
Meiosis – Result
■ primary
spermatocyte gives
rise to 4 daughter
cells which develop
into 4 mature
gametes
■ 2 = 22 + 1X
chromosome
■ 2 = 22 + 1Y
chromosome
Clinical Correlates
1. Meiotic Nondisjunction
■ no separation occurs
■ 1 cell receives 24 chromosomes and the
other 22
■ At fertilization, normal gamete of 23 fuses
with abnormal gamete producing either
monosomy 45 or
trisomy 47
Clinical Correlates
2. Mitotic Nondisjunction
■ involves chromosome 21 (Down
Syndrome)
■ Mosaicism – few or many
characteristics of Down Syndrome
depending on the # of cells involved
Clinical Correlates
3. Balanced Translocation
■ breakage and reunion of chromosomes
between 2 chromosomes
■ no critical genetic material is lost
■ normal individual
4. Unbalanced Translocation
■ part of 1 chromosome is lost
■ produces an altered phenotype
Clinical Correlates
Spermatogonia
(shortly before puberty, sex cords acquire a lumen=
seminiferous tubules)
Type A Type B
Primary Spermatocyte
meiosis I
Secondary Spermatocyte
meiosis II
Spermatid (haploid)
spermiogenesis
Spermatozoa
Spermiogenesis
■ series of changes
resulting in the
transformation of
spermatids into
spermatozoa
1. Formation of the
acrosome
2. Condensation of the
nucleus
3. Formation of neck,
middle piece and tail
4. Shedding of most of
the cytoplasm
Oogenesis
Oogonia
mitosis
Follicular Cells
mitosis
Primary Oocyte
meiosis
Primordial Follicle
Oogenesis – Postnatal Maturation
Primary Oocyte
-near time of birth = 700T-2M
-starts prophase of the 1st meiotic division
Primary Follicle
Oogenesis – Postnatal Maturation
Primary Follicle
(theca folliculi, zona pellucida)
Secondary Follicle
(antrum, cumulus oopherus)
Tertiary Follicle
= Vesicular or Graaffian Follicle
meiosis I
Secondary Oocyte
+ 1 Polar Body
Ovarian Cycle
= sexual cycle
■ regular monthly cycles of females at
puberty
■ controlled by the hypothalamus
(gonadotropin-releasing hormone)
anterior pituitary gland (gonadotropins
= LH and FSH) stimulate and
control the cyclic changes in the ovary
Estrogen
Stigma
• an avascular spot at the apex of the ovary
• point of rupture of the oocyte during
ovulation
• due to:
1. local weakening and degeneration of the
ovarian surface
2. increase intrafollicular pressure
3. muscular contraction of the ovarian wall
Ovulation
Signs of Ovulation:
1. Middle pain or Mittlesmerz
2. Rise in basal body temperature
3. Thinning of the cervical mucus
Ovulation
B. Acrosome Reaction
- occurs after binding of the sperm to the zona pellucida
of the oocyte
- induced by the zona proteins
- culminates in the release of lysosomal enzymes
(including acrosin and trypsin-like subs.) needed in the
penetration of the zona pellucida (zona reaction)
Phases of Fertilization
Phase I
Penetration of the Corona Radiata
Phase II
Penetration of the Zona Pellucida
Phase III
Fusion of the Oocyte and Sperm
Cell Membrane
3. Initiation of cleavage
Clinical Correlates
Methods of Contraception:
1. barrier techniques – condoms,
diaphragm
2. oral contraceptives or pills
3. Depo-provera – subdermal implant or
intramuscular injection
4. IUDs
5. Vasectomy or tubal ligation
Clinical Correlates
2-cell stage
mitosis
Blastomeres
(increase in cell number)
8-cell stage
16-cell stage
(morula)
Cleavage
Morula
(In the uterine cavity
3 days after fertilization)
blastocyst
embryoblast trophoblast
6th day
implantation
Implantation
blastocyst
embryoblast trophoblast
6th day
implantation
Trophoblast
1. Cytotrophoblast – inner,
mononucleated
2. Syncytiotrophoblast – outer,
multinucleated
Embryoblast
• Exocoelomic or
Heuser’s membrane
• Exocoelomic Cavity
= Primitive Yolk Sac
Uteroplacental Circulation
■ sinusoid (maternal capillaries) eroded by the deep
penetration of the syncytiotrophoblast into the
endometrial stroma
■ establishes maternal blood flow to the embryo
th
13 Day AOG
Ectopic Pregnancy
• extrauterine pregnancy
Hydatidiform mole
• trophoblast develops placental membrane
but little or no embryonic tissue
• benign or malignant (invasive mole,
choriocarcinoma)
• arise from fertilization of an oocyte lacking
in nucleus followed by duplication of the
male chromosome to restore the diploid #
• suggests that paternal genes regulate most
of the development of the trophoblast
Gastrulation
Ethanol
▪ kills cells in the antero-midline of the
germ disc
▪ deficiency of midline craniofacial
structures
▪ eg. holoprocencephaly
Clinical Correlates
Serenomelia
= Caudal Dysgenesis
▪ due to insufficient
mesoderm formed in
the caudalmost region
of the embryo
▪ contributes to lower
limbs, urogenital
system & lumbosacral
vertebrae
▪ risk factor- maternal
diabetes mellitus
Clinical Correlates
Sacrococcygeal Teratoma
▪ remnants of the
primitive streak
persists in the
sacrococcygeal area
▪ contains tissues
derived from the 3
germ layers
▪ most common tumor in
the newborn
Development of the Trophoblast
Primary villi
Secondary villi
Tertiary villi
= definitive placental villus
-differentiation of core into
blood cells & small vessels
forming the villous capillary sys
-establish contact with the
intraembryonic circulatory sys
connecting placenta & the embryo
Development of the Trophoblast
Umbilical Cord
▪ establishes attachment between the
embryo and the placenta as a narrow
connecting stalk at its trophoblastic
shell on Day 19-20 of development
Embryonic Period
Paraaxial Mesoderm
Lateral Plate – divided into 2 layers:
1. somatic or parietal mesoderm – lateral &
ventral body wall
2. splanchnic or visceral mesoderm – wall of
the gut
Intermediate Mesoderm – forms the excretory
units of the urinary system & the gonads
1. nephrotomes – cervical & upper thoracic
regions
2. nephrogenic cord – caudal regions
Somitomeres
forces the blood deep into the intervillous spaces with oxygenated blood
pressure decreases & blood flows back from the chorionic plate
toward the decidua where it enters the endometrial veins
Functions of the Placenta
1. exchange of gases
2. exchange of nutrients & electrolytes
3. transmission of maternal antibodies
4. hormone production
5. protective barrier against damaging
factors
6. detoxification of some drugs
Functions of the Placenta
Gas Exchange
• simple diffusion = O2, CO2 and CO
• at term, fetus extracts 20-30 ml
O2/min from the maternal circulation
• the amount of O2 reaching the fetus is
primarily dependent on placental
blood flow and not diffusion
Functions of the Placenta
Transmission of Maternal
Antibodies
• maternal Ab taken up by the pinocytes
by the syncytiotrophoblast then
transported to fetal capillaries
• mainly IgG class
• provides passive immunity against
diphtheria, smallpox and measles
(fetus has little capacity to produce its
own Ab until after birth
Functions of the Placenta
(Clinical Correlates)
Erythroblastosis fetalis or Hemolytic Disease of
the Newborn
• Fetus = Rh (+) Mother = Rh (-)
• fetal RBC invading the maternal bloodstream may elicit
an Ab response in the mother intrauterine death of
nd
2 child
• diagnosed by analysis of the amniotic fluid
• prevented by: intrauterine blood transfusion or exchange
transfusion after birth; injection of Rh immunoglobulin
(Rhogam) to the mother
Protective Barrier
• viruses which traverse the placenta
without difficulty = rubella (measles),
rubeola (German measles), variola
(smallpox), varicella (chickenpox),
coxsackie, CMV, poliomyelitis
Detoxification of Some Drugs
- fetal drug addiction = heroin
Umbilical Cord
■ 2 arteries and 1 vein
■ Physiologic umbilical hernia – the abdominal
cavity is temporarily too small for the rapidly
developing intestinal loops and some are pushed
into the extraembryonic coelomic space in the
umbilical cord
■ Jelly of Wharton – proteoglycan-rich subs.
which surrounds the umbilical cord & serves as a
protective layer to the blood vessels
■ approx. 2 cm diameter & 50-60 cm long, tortous
– extremely long cord coil fetal asphyxia
– short difficult attachment of the placenta
Amniotic Bands
■ due to tears in the
amion
■ scar-like tissue
constricting part of the
fetus (limbs or digits)
■ origin of the bands is
probably infections or
toxic insults that
involve either the fetus,
fetal membrane or
both
■ may result in :
amputations, ring
constrictions,
craniofacial deformities
Placental Changes at the End of
Pregnancy (indicates reduce
exchange)
1. increase fibrin tissue in the core of
the villus
2. increase thickness of the basement
membrane in the fetal capillaries
3. obliterative changes in small
capillaries of the villi
4. deposition of fibrinoid on the surface
of the villi in the junctional zone & in
the chorionic plate
Amniotic Fluid
Twins
• higher incidence of perinatal morbidity
and mortality
• high tendency toward preterm labor
and low birth weight
Clinical Correlates
Vanishing Twin
• refers to death of 1
fetus
st
• occurs in the 1
trimester or early
2nd trimester
• due to resorption or
formation of fetus
papyraceus
Clinical Correlates
Twin Transfusion
Syndrome
• occurs in 5-15% of
monochorionic,
monozygotic twins
• placental vascular
anastomoses are formed
such that 1 twin receives
most of the blood flow
while the other is
compromised
• results in larger 1 twin
• poor outcome (death of
both twins in 60-100% of
cases)
Clinical Correlates
Conjoined Twins
= Siamese Twins
• incomplete splitting of the axial area of the
germ disc
• classified accdg. to nature & degree of union
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