ABSTRACT

Sterilization of a process to eliminate, kill or destroy all forms of living

myroorganisms, whether pathogenic or not, either in vegetative or spore form of an object or
material. Washing and sterilizing is carried out with the aim that microorganisms in the
preparation equipment and packaging can be sterile and not contaminated with other
microorganisms from outside. There are three types of sterilized equipment, including
glassware with steam sterilization method, metal tools with dry heat sterilization method and
rubber tools with aseptic sterilization method. Sterility tests were carried out in a study to
determine the growth of bacteria before and after the sterilization process. Based on the
results of research, bacterial growth cannot be detected.

Keywords: sterilization method, sterility test, sterile instrument