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Tran Thi Ngoc Son , Vu Van Thu , Duong Van Chin and H. Hiraoka
Abstract
The field experiment on soybean was conducted at Phuoc Thoi village- O Mon district- Cantho province
with different fertilizer doses application to study the influence of organic and bio- fertilizer on the growth
and grain yield of soybean and soil fertility. The experimental results showed that the application of
organic and bio -fertilizer could be substantiated for the N inorganic fertilizer to an extent of 40 kg N ha-1
while the agronomic characteristic and grain yield of soybean were comparable to the control
(conventional dose applied by farmers). The contents and uptake of soybean with reference to N, P, and K
and soil available P and K were significantly improved by the application of composted paddy straw and
inoculants viz., SB 83 (Rhizobium fredii) and SB 177 (Bradyhrizobium sp). The highest N use efficiency
by rice obtained under succeeding of treatment composted paddy straw.
1. Introduction
Farming practices which involve heavy application of chemical fertilizers may cause depletion of certain
nutrients in soil and certain others would generally accumulate in excess resulting in nutrient imbalance
which effects the soil productivity. Among the means available to achieve sustainability in agricultural
production, organic manure and bio-fertilizer play an important and key role because they possesses many
desirable soil properties and exerts beneficial effect on the soil physical, chemical and biological
characteristics of the soil. However the most optimum organic and bio-fertilizer doses as well as their
effectiveness for upland crops have not been studied in details. In this regard an attempt has been made to
study on the influence of organic and bio-fertilizer on soybean under rice based cropping system.
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Table 1 : Treatment structure
The NPK were applied in the form of urea, single super phosphate and muriate of potash , respectively.
The composted paddy straw was incoporated in the soil one day prior to sowing at the rate of 2 t / ha . The
inoculants viz., SB 83 (Rhizobium fredii) and SB 177 (Bradyhrizobium sp.) were inoculated with soybean
seeds at the ratio of 1:10 (1g inoculants and 10 g of soybean seeds). One third of the N and full dose of
P2O5, K2O were applied basally. The remaining two third dose of N was applied at 20 th and 35 days after
sowing (DAS). The soybean seeds were sown adopting a plant spacing of 40 x 20 cm, 3 plants/hill (plant
population = 375,000 plants/ha). The residual crop viz., rice was sown as such without disturbing the lay
out adopting a seed rate of 100 kg ha-1 by row seeding. Cultural practices and plant protection measures
were followed as per the packages of practice to soybean and rice crops as conventional recommendation.
Plant height, no. of soybean leaves and SPAD value were measured at two weeks interval beginning from
two weeks and four weeks after sowing, respectively.
Collection of soil samples: Composite representative soil samples were collected from the field for
evaluating initial analysis. Then the soil samples were collected from each plot at harvest stage for
soybean. The soil samples were air dried and gently beaten with a wooden mallet and sieved through two-
mm nylon sieve and stored in polythene bags.
Collection of plant samples: Plant samples were collected at the harvest stage for soybean. The plant were
washed with diluted H2SO4 at 0.1 % rinsed with distilled water, air-dried and then oven dried at 60 oC for
12 hours. The dried samples of straw and grain were ground in mechanical grinder and stored. The
samples thus prepared were taken for the chemical analyses. Uptake of nutrients viz., N, P, K was
calculated by multiplying the content of the nutrients (expressed in percentage) with the respective
weights of dry matter of the plant samples at the harvesting stage. The data obtained from the present
investigation were subjected to statistical scrutiny following the methods of Snedecor and Cochran
(1967) and Panse and Sukhatme (1967) and the results were interpreted
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3. Results and discussions
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among treatments and it was on a par with T7 (Inoculants +30-30-30 ) and T1 (100-60-30) . This may
be due to the activity of Rhizobium fredii microorganism helping in enhancement of N fixation process
In general the electrical conductivity of soil was marked influenced by treatments Howerever the EC of
soil was attended at suitable level for crop growth.
Organic carbon: It was observed that the maximum value obtained under treatment T8 (C+60-60-30) and
significantly different from control T1 (100-60-30). A build up of organic carbon due to organic waste
+NPK application from 0.034 to 0.067 % might be due to the inorganic fertilizer supplying the needed
energy and nutrient for the decomposition of complex organic matter and converting them to mineralized
organic colloids which are added to the soil organic matter reserves and rapid multiplication in the
microbial population.
Soil available nitrogen: Marked changes were observed on soil available nitrogen due to influence of
treatments. Higher soil N value observed under T8 (C+60-60-30). This was due to the inherent N content
of the waste material incorporated and transformation during composting and after application in the soil.
The available P was found to be higher under T8 (C+60-60-30) and T9 (C+30-60-30). The organic acids
released during decomposition of organic influences the pH from stable complexes or chelates with
cations responsible from P fixation and inturn increases P availability. The application of organic manure
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significantly reduced the fixation of added as well as native P, making P more available to plant. (Ghosh
et al., 1981, Son and Ramaswami, 1997).
2.50
2.00
1.50
1.00
0.50
0.00
0
30
30
30
30
30
00
0
0
0
3-
-3
-3
-3
-0
-3
0-
0-
0-
0-
0-
0-
0-
60
60
60
00
60
-6
-6
-6
-6
-6
-6
-0
0-
0-
0-
0-
0-
60
60
30
00
60
30
00
+3
+6
+3
+0
10
I+
I+
I+
+I
C
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5) Nutrient content in soybean grain (Table 6)
Nitrogen content in grain: the N content in soybean grain was the highest due to the application of
composted paddy straw + 60-60-30 kg NPK (T8). The treatment T1 (100-60-30) registered the lowest N
content application of composted paddy straw and inoculants incresead the N content in soybean by 5.26
to 9.78 % over control for T10 (I+00-00-00) and T8 (C+60-60-30) , respectively. (Fig. 2)
N content 5.9
(%)
5.8
5.7
5.6
5.5
5.4
5.3
5.2
5.1
5
T1 T2 T3 T4 T5 T6 T7 T8 T9 T10 T11 T12
Treatments
Fig.2 Treatmental influence on N content in
soybean grain
Nitrogen content in straw: the mean value of N content in soybean straw varied from 0.6629 to 0.7367 %.
The lowest value recorded under treatment T1 (100-60-30). There was no significant different between
treatments. The application of composted paddy straw and inoculants incresead the N content in straw of
soybean by 1.21 to 9.84 % over control for T12 (C+I+30-60-30) and T8 (C+60-60-30), respectively.
Phosphorus content in grain: the P content in soybean grain was the highest due to the application of
composted paddy straw+ 30-60-30 kg N -P2O5K2O (T9). The treatment T1 (100-60-30) registered the
lowest P content Application of composted paddy straw and inoculants increased the P content in
soybean by 0.86 to 9.86 per cent over control for T10 (I+00-00-00) and T9 (I+ 30-60-30) , respectively.
Phosphorus content in straw: the mean value of P content in soybean straw ranged from 0.1013 to 0.1333
per cent. The was the lowest value recorded under treatment T11 (C+00-00-00). The application of
composted paddy straw and inoculants incresead the P content in straw of soybean by 2.70 to 21.5 per
cent over control for T12 (C+I+30-60-30) and T9 (I+ 30-60-30) , respectively.
Potassium content in grain: the K content in soybean grain was the highest due to the application of
composted paddy straw+ 30-60-30 kg NPK (T9). The treatment T1 (100-60 –30) registered the lowest K
content. Application of composted paddy straw and inoculants incresead the P content in soybean by
11.88 to 23.81 per cent over control for T6 (I+60-60-30) and T9 (I+ 30-60-30) , respectively.
Potassium content in straw: the mean value of K content in soybean straw ranged from 2.073 to 2.323 per
cent. The was the lowest value recorded under treatment T11 (C+00-00-00) . There was no significant
increment due to application of composted paddy straw and inoculates, probably due to low dose of
composted paddy straw.
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Table 4 : Treatmental influence on soil nutrient availability of soybean at harvest stage
C: composted paddy straw; I : Inoculants SB 83 (Rhizobium fredii) and SB 177 (Bradyhrizobium sp)
The treatment means with the same letter are not significantly different using LSD at 5 %
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Table 6 : Treatmental influence on nutrient content (%) in grain and straw of soybean crop
P uptake: Significant differences were noticed in uptake of P due to treatments. The highest P content
was observed under application of composted paddy straw (T8). An increase of 6.74 % for P uptake over
NPK alone T1 (100-60-30). This is in accordance with the finding of Sharma and Mittra ,1991
K uptake :Marked variation were observed in K uptake by soybean crop due to influence of treatments.
There were significant differences in the uptake among treatments. Application of Inoculants and
composted paddy straw had substantially higher K uptake. The uptake of K was enhanced to the extent of
17.76 % over T1 (100-60-30). This result is also in harmony with of Sharma and Mittra ,1990.
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Table 7 : Treatmental influence on nutrient uptake of soybean grain at harvest stage (Kg/ha)
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Table 8 : Treatmental influence on the yield , yield components and N use efficiency of succeding rice crop
S. Soybean Rice No. of No. of Unfilled 1,000 Grain yield N use efficiency
No. (Spring- Summer) (Summer- panicle filled grain grain (T ha-1) (KgN / Kg rice)
- 2
Autumn) m grains/ percentage weight
panicle (%) (g)
T1 100- 60 –30 70-60-30 425 a 45.37 a 16.53 a 25.30 a 2.613 d 37.32
T2 60 - 60 - 30 70-60-30 433 a 45.97 a 17.30 a 25.20 a 2.527 cd 36.10
T3 60 - 60 – 30 50-40-30 385 a 45.77 a 19.03 a 25.27 a 2.037 ab 40.74
T4 30 - 60 –30 50-40-30 399 a 44.60 a 17.57 a 25.13 a 2.073 ab 41.46
T5 00-60-30 50-40-30 385 a 42.70 a 18.97 a 25.27 a 2.067 ab 41.34
T6 I+60-60-30 50-40-30 388 a 43.67 a 17.87 a 25.30 a 2.287 bc 45.74
T7 I+30- 30-30 50-40-30 380 a 44.97 a 18.07 a 25.20 a 2.187 ab 43.74
T8 C+60-60-30 50-40-30 392 a 44.67 a 21.20 a 25.53 a 2.280 bc 45.60
T9 C+30 –60-30 50-40-30 369 a 43.53 a 16.47 a 25.40 a 2.267 bc 45.34
T10 I +00 –00-00 50-40-30 380 a 40.93 a 19.83 a 25.07 a 2.073 ab. 41.46
T11 C+00 – 00- 00 50-40-30 389 a 41.17 a 19.37 a 25.37 a 1.947 a 38.94
T12 C+I +30 -60-30 50-40-30 397 a 42.20 a 19.30 a 25.50 a 2.113 ab 42.26
LSD 5% 14.3 6.58 4.27 0.690 0.233
CV % 95.55 8.90 13.7 1.6 6.10
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4. Conclusions
The fertilizer dose for soybean could be at the rate of 60N- 60 P205- 30 K20 combined with composted
paddy straw or inoculants or both composted paddy straw and inoculants which could be obtained the
same yield and agronomic characteristics of soybean as that of the rate of higher dose of inorganic
fertilizer as T1 (100-60-30) as farmer dose application. At the same time, the experimental results
showed that the application of organic and bio -fertilizer could be substantiated for the N inorganic
fertilizer to an extent of 40 kg N ha-1 while the agronomic characteristic and grain yield of soybean
were comparable to the control (conventional dose applied by farmers). The contents and uptake of
soybean with reference to N, P, and K and soil available P and K were significantly improved by the
application of composted paddy straw and inoculants viz., SB 83 (Rhizobium fredii) and SB 177
(Bradyhrizobium sp). The highest N use efficiency by rice obtained under succeeding of treatment
composted paddy straw.
Literature Cited
Ghosh, SC, PK Omanwar,RS Sachen and RB Sharma.1981. Reserves of organic and total phosphorus
in cultivated and Brorested mothosols. J. Indian Soc. Soil Sci.., 29: 332-336
Narayanamma, MS, Rami Reddy, MG Ramakrishna Reddy and P. Ramachadra Reddy.1985. Studies
on improving the productivity of groundnut on problem soils. Andhra agric. J.32: 6-8
Ramaswami,PP and TTN Son. 1996. Quality compost from agricultural wastes. Paper presented at the
national workshop on “ Organic farming for sustainable agriculture”. Water and land mangement
training and Research Institute- Hyderabad, India.
Sharma AR and BN Mittra.1988. Effect of combinations of organic materials and nitrogen on uptake
of rice. J. Agric. Sciences: 495-501
Sharma AR and BN Mittra.1990. Complementary effect of organic , bio and mineral fertilizer in rice
based cropping system. Fert. News., 35(2): 43-51
Sharma AR and BN Mittra.1991. Direct and residual effect of organic materials and phosphorus
fertilizer in rice based cropping system. Indian J. Agron.,36 : 299-303
Son, TTN and PP Ramaswami. 1997. Effect of organic wastes application on physical and chemical
properties of heavy clay soil .Omon Rice 5 :48-55
Thanikachalam, A and Ragarajan. 1992. Bioconversion of rice straw into protein rich feed. Madras
Agric.J. 79 (3): 38-141
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