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CÁLCULO MIX DE PCR

no. de amostras 1
Volume Total da Reação

Mix 0 0
Forward 0 0
Reverse 0 0
DNA
Água 0 0

volume por tubo #DIV/0!


DNA por tubo 0
Total por tubo #DIV/0!
µL de sobrenadante µL de isopropanol
100 60
200 120
300 180
400 240
500 300
600 360
700 420
800 480
900 540
1000 600
conversão entre rpm e g p rotor de 7,51 cm
G RPM
300 1890.2522212726
600 2673.2203276295
1000 3451.1126032174
1500 4226.7324613854
2000 4880.6102487468
2500 5456.68814394
3000 5977.5023714139
3500 6456.4404822086
4000 6902.2252064349
4500 7320.9153731202
5000 7716.9223788005
5500 8093.5764715282
6000 8453.4649227709
6500 8798.6452537059
7000 9130.7856945941
7500 9451.2611063628
8000 9761.2204974936
8500 10061.6357865276
9000 10353.3378096523
9500 10637.0434285481
10000 10913.37628788
10500 11182.8829512298
11000 11446.0456141389
11500 11703.2922419716
12000 11955.0047428278
12500 12201.525621867
13000 12443.1634483005
13500 12680.1973841563
14000 12912.8809644172
14500 13141.4452744457
15000 13366.1016381477
15500 13587.0439059177
16000 13804.4504128697
16500 14018.4856636308
17000 14229.3017889658
17500 14437.0398108999
18000 14641.8307462404
18500 14843.7965730333
19000 15043.0510802044
19500 15239.7006171933
20000 15433.8447576009
20500 15625.576888607
RPM G
1000 7.556562
1200 30.22625
1400 83.9618
1500 188.9141
2000 335.8472
2500 524.7613
3000 755.6562
3500 1028.532
4000 1343.389
4500 1700.226
5000 2099.045
5500 2539.844
6000 3022.625
6500 3547.386
7000 4114.128
7500 4722.851
8000 5373.555
8500 6066.24
9000 6800.906
9500 7577.552
10000 8396.18
10500 9256.788
11000 10159.38
11500 11103.95
12000 12090.5
12500 13119.03
13000 14189.54
13500 15302.04
14000 16456.51
14500 17652.97
15000 18891.41
15500 20171.82
16000 21494.22
16500 22858.6
17000 24264.96
17500 25713.3
18000 27203.62
18500 28735.93
19000 30310.21
19500 31926.47
20000 33584.72
20500 35284.95
Cálculo proteinase K/nº de amostras
Nº de amostras SDS 10% (µL) PK (µL)
1 70 6
2 140 12
3 210 18
4 280 24
5 350 30
6 420 36
7 490 42
8 560 48
9 630 54
10 700 60
11 770 66
12 840 72
13 910 78
14 980 84
15 1050 90
16 1120 96
17 1190 102
18 1260 108
19 1330 114
20 1400 120
21 1470 126
22 1540 132
23 1610 138
24 1680 144
25 1750 150
26 1820 156
27 1890 162

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