Catalogo CECIL

ADVANCED - LOW COST - UV / VIS
High Quality Low Cost

When cost is a primary consideration of selection, the
Series 1000 provides low cost, high specification,
quality instruments for both the UV and visible
ranges.
The new lightweight designs offer an ideal

combination of performance, quality and reliability for
educational use; as is witnessed by the large number
of Universities, Polytechnics and Colleges who are
Ease of Operation
multiple users of the range.
With only six, carefully programmed operating keys,
Wavelength Precision the Series 1000 spectrophotometers are exceptionally
easy to use. Up-down keys, with automatic slew rate
Very precise wavelength selection, with readout to acceleration, make wavelength selection, to 0.1nm,
0.1nm, provides the high degree of reproducibility convenient and rapid. Absorbance, Transmittance or
needed for accuracy, particularly when measuring on Concentration may be measured; the latter using
a sloping background. standards or by entering a factor.
Convenient Operation Deuterium Lamp Save

Design advantages of the Series 1000 instruments UV-visible instruments allow the Deuterium lamp to
include self test and calibration at switch on, be switched off when working in the visible region, to
automatic lamp and filter selection coupled to extend lamp life. Switch on is automatic if a UV
wavelength and high overall stability eliminating wavelength is selected. Lamps are guaranteed for
frequent resetting of the absorbance zero when 1500 hours and are fitted with an hours of use
making extended measurements. indicator.
2
FLEXIBLE SAMPLE HANDLING
Peak Seek Sample Temperature Control USER BENEFITS
The measurement of an Temperature control by water Easy Use
absorption band should always be circulation is possible with the
carried out at the wavelength of Series 1000 for a single cell, SIP
Low Cost
peak absorption to obtain results flowcell, or for 4 cells mounted on
of the highest accuracy and the cell changer. A special sample
Self Test and Calibration
reproducibility. compartment lid and pip port are
provided for use with water
An automatic peak seek facility is temperature control. Auto Lamp and Filter
provided. To enhance dis- Selection
crimination and accuracy
automatic compensation of the Convenient Sampling Simple Kinetics
reference energy background -
The large and accessible sample
baseline flattening - is carried out, UV Lamp Save
compartment accommodates a
followed by precise peak location.
wide range of sample holders in
The instrument is set to the peak
addition to the standard single cell UV Lamp Hours Indicator
wavelength, ready for use.
holder. These include a 4 cell
changer, water thermostatted cell 1500 Hours UV Lamp
holders and a SIP sampling system Guarantee
which fits within the sample
compartment.
SIP Flowcell Sampling SAMPLING

Used with Series 1000 instruments
Single Cell Holder
the SIP sampling accessory allows
samples to be measured without
the inconvenience of cell handling, 4 Cell Changer
or danger of cell breakage.
Samples are drawn into the cell by 4 Microcell Changer
depressing the sample key on the
control panel, to displace the SIP Flowcell Changer
previous sample.
Cell Temperature Control
The pump unit is mounted within
the cell compartment. Cells of up to
100mm Cell Holders
40mm pathlength are available and
samples as small as 300µl may be
measured in a 10mm pathlength 50µl Cell Holder
cell.
Film or Filter Holder
3
ORDERING ACCESSORIES
SAMPLE HOLDERS Part No. SAMPLE STIRRING Part No.
Single cell holder 1010 31 00 Electronic controller for sample stirring 2010 31 00
Universal cell holder, 10mm cells upwards 1010 38 00 Cell holder for sample stirring 2021 32 00
Long pathlength cylindrical cell holder 1010 39 00 Water thermostatted holder for stirring 2010 33 00
Holder for solid samples or films 2020 33 00 LAMPS
Ultra-microcell holder : 50µl or less 2020 39 00 Deuterium lamp with hours indicator 2202 01 42
CELL CHANGERS AND HOLDERS Tungsten halide lamp - in pairs 2303 01 40
4 cell manual changer (no holder) 1010 34 00 CABLES AND COMPUTERS
4 x 10mm cell holder for manual charger 1010 24 00 RS232c cable for PC with 25 pin outlet and
Up to 4 x 50mm holder for manual charger 1010 36 00 protocol manual 2021 26 00
4 microcell manual changer and holder 2020 38 00 RS232c cable for PC with 9 pin outlet and
SIP SAMPLING protocol manual 2021 83 00
Holder for 10mm SIP cell 1010 32 00 CALIBRATION FILTERS
Holder for SIP cell up to 50mm 2020 37 00 Holmium filter in holder 202 01 44
THERMOSTATTING Didymium filter in holder 303 01 30
Water flow holder for manual 4 cell changer 1010 36 15 Set of 4 certified absorbance filters in holders 594 44 00
Water flow holder for single, SIP cell 1010 33 00 Set of 6 certified absorbance filters in holders 594 66 00
Cell compartment lid and water pipe port 1010 05 00 Set of 2 certified wavelength filters in holders 303 40 00
Specification ORDERING
Optical System Littrow monochromator using 1200L/mm
Spectrophotometers are supplied
holographic grating; coated optics
complete with single cell holder,
Optical Bandwith 8nm
power cable, operators manual and
Wavelength Selection Keyboard controlled by up-down keys short form instructions.
Wavelength Range 200-1100nm CE1021
325-1100nm CE1011
Wavelength Accuracy Within 1nm VISIBLE RANGE
Wavelength Precision 0.1nm
Wavelength Display Large seven segment bright LED’s CE 1011
Photometric Ranges -0.3 to 3.0A, 0-200%T, 0-9999C 325-1100nm Wavelength range
8nm Bandpass
Photometric Accuracy 1% or 0.005A whichever is greater
Photometric Noise Less than + 0.001A/Hr.
Straylight Less than 0.05% at 220nm and 340nm CE1021
Absorbance Zero Stability Better than + 0.001A/Hr. UV/VISIBLE RANGE
Absorbance Zero Setting 0A and 100%T automatic by press button
CE 1021 Spectrophotometer
Peak Seek Automatic with baseline flattening
200-1100nm Wavelength range
Filter and Lamp Selection Automatic - coupled to wavelength 8nm Bandpass
Self Test and Calibration Automatic at switch on
Computer Interface Bi-directional serial RS232c Option F
Sample Compartment Dimensions 196 x 105 x 107mm (l x d x h) Sip Sampling : Factory fitted only
Size 480 x 340 x 200mm (l x d x h)
System 1
Power Requirement 110-250V, 50/60Hz, 120W CE1010, 170W CE1021 Spectrophotometer (with option), 2
Weight 10kg CE1011; 17kg CE1021 cells, spare lamp(s), spare fuses
Cecil Instruments policy is one of continuous development. We therefore reserve the right to change specification without notice.
CECIL INSTRUMENTS LIMITED MILTON TECHNICAL CENTRE CAMBRIDGE CB24 6AZ ENGLAND
TELEPHONE: 01223 420821 FAX: 01223 420475 ISO 9001 : 2000
CERTIFIED COMPANY
E-MAIL: info@cecilinstruments.com WEB SITE: www.cecilinstruments.com
4
UV-VISIBLE SPECTROPHOTOMETERS
The Choice
Aurius Series Super Aurius Series
With an optical bandwidth of 4nm and superb stability, The Super Aurius instruments are unique for their
accuracy and precision these very easy to use very high performance scanning, with derivatives,
instruments are ideal general purpose overlay, extended analytical power and multi-cell
spectrophotometers. Kinetics. They cover the full 190-1100nm wavelength
Excellent for routine and quality control laboratories, range with a narrow 1.8nm optical bandwidth.
they offer a wide range of techniques with wavelength With straylight of 0.01%, wavelength precision of
scanning as an option. Both UV and Visible versions 0.1nm and baseline stability better than ±0.001A/hr,
are available with full validation procedures. the most demanding analysis may be undertaken.
Performance with Convenience Ease of Operation

The ease of operation makes even complex tasks The large screen displays all menus and prompts. All
simple. An exceptional range of software and curves, scans and data are screen displayed and may
accessories makes the Aurius and Super Aurius be reprocessed before printing.
instruments superb multi-user instruments, ideal for
multi-discipline laboratories. Software Enhancement by E-SEF
E-SEF - Encoded Software Enhancement Facility - is an
Absorbance Accuracy important new Cecil design feature. Optional software
The low straylight of the instruments, less than 0.01%, modules are enabled by entering correctly allocated
allows a measurement of 3 Absorbances to be made encoded numbers. These numbers can be provided by
with an error close to 1%. This should be compared telephone, fax or E-Mail. Exact software requirements
with errors of 26%, 10% and 6% which result from may therefore be tailored after instrument purchase
straylight of 0.5%, 0.1% and 0.05% respectively for the without incurring delay before use.
same measurement.
Integral Printer
Six Screen width Viewing The integral printer versions provide hard copy of
All scans, curves and data produced by the spectra, curves and time course plots, as well as timed
spectrophotometers may be scrolled across the screen and dated reports of all experiments. An external,
providing a very wide 6 screen width - 550mm - for Epson compatible, printer may be used providing large
viewing of extended displays. high quality plots and documentation of assays.
2
SIX SCREEN WIDTH VIEWING
Accurate Quantification
For optimum accuracy when measuring concentration, a calibration
line may be fitted to a series of up to 30 standards or replicates.
Accuracy is improved over using a single known concentration.
Concentration Curve Fitting

When concentration is not linearly related
to Absorbance a quadratic or cubic curve
may be fitted to up to 30 standards or
replicates, with or without an intercept. A
straight line may be fitted when a linear
relationship exists. Curves are displayed
with data for each standard documented.
Editing of Data
Suspect standards revealed by the initial fit
may be deleted or replaced for a new fit. Up
to 30 curves may be stored security
protected.
Wavelength Scanning
Wavelength scanning from 190-1100nm is standard
on Super Aurius instruments with a narrow 1.8nm
optical bandwidth. For the Aurius range scanning
software is optional with a 4nm optical bandwidth.
Scans at up to 4000nm/min are displayed with

peaks and valleys tabulated and scans of up to 6
screen widths may be viewed by scrolling.
Spectra may be printed on either the integral
printer, as shown below, or an external printer.
The Super Aurius range offers in addition:

Scanning, derivitatives and time plots as standard.
Spectral storage, overlay, band quantification,
stripping and differencing are also available.
Cell and Wavelength Programming

A cell program software module enables up to 4, 6 A combined cell and wavelength program may be run for
or 8 cells to be programmed with the appropriate up to 8 cells and 10 wavelengths.
cell changer. A cell and wavelength program may be used for
Programming of up to 10 wavelengths is also calculations using complex formulae entered using the
provided by the wavelength program software Assay program. Results are automatically formatted and
module. printed.
3
FAST : EASY TO USE :
SCANNING QUANTITATION
NOTE: This column applies only to Super Aurius.
Scan is an option on the Aurius range.
Wavelength Scan Assay Program

Scans at up to 4000nm/min are presented on screen or Formulae for measurements at up to 10 wavelengths and 8
printed with all peaks annotated with wavelength and cells may be entered rapidly. Programming and formatting of
Absorbance. Tables are also presented listing all peaks and results is automatic at entry without user intervention. Multi-
troughs with values. sample assays may be stored as methods.
Derivative Spectra Concentration Measurement

Derivative spectra are readily produced, with selectable In the concentration measurement mode samples may be
smoothing, either alone or superimposed on the measured either by using a calibration standard, or entering
fundamental, with or without an offset. Scaling is automatic, a known factor relating concentration to Absorbance.
with all peaks and troughs quantified.
Overlayed Spectra Curve and Line Fitting

Up to six scans, including stored scans, may be overlayed, A straight line, quadratic or cubic curve may be fitted to up to
with or without offsets for convenient comparisons or further 30 standards, with or without an intercept at the origin.
manipulation. Standards may be deleted and replaced and a new curve fitted.
Dynamic Scan Recall Reaction Kinetics

The last eight scans are automatically retained for recall by Single and multi-cell kinetic measurements are possible with
selection from a list and may be reprocessed, overlayed or plots and data displayed on screen. Data may be reprocessed
transferred to protected memory for future use. and curves stored, security code protected against tamper or
deletion.
Difference Spectra Multi Wavelength Assays
New scans or scans from store may be presented on screen Assays are provided using either two or three wavelengths.
and the difference spectra produced. The scans may be A wine colour assay is provided which may use up to ten
normalised enabling spectral stripping to reveal impurities or different wavelengths. Multi-wavelength computations are
additives. possible up to 10 wavelengths.
Band Area Height Computation DNA and Protein Assays

Band areas with correction for sloping background Protein analyses may be made using either the 2 or 3
absorption are readily quantified, with all details and wavelength methods of Warburg and Christian. Also
construction displayed. Band areas may also be computed programmed are techniques and analytical procedures for
for derivative spectra to facilitate difficult quantification. assays by Lowry, Bradford, Biuret and BCA methods.
Scan Storage Ratio and Difference

Spectra may be stored in safe memory for future recall. Each Absorbance ratioing at 280/260nm, or two user selected
spectrum is automatically allocated a recall number, when wavelengths, is provided for DNA purity assays. The scaled
stored, and may be security code protected against tamper difference of Absorbance values at two wavelengths is also
or deletion. provided.
Programmed Scans Cell Program

Scans, automatic, single or repeat may be made of a cell Using the various cell changers, measurements may be
program of up to 4, 6 or 8 cells. A programmed sequence of made on up to 4, 6 or 8 cells at a single wavelength with cycle
scans may also be made of a single sample. time and number of cycles entered by the operator. A cell
and wavelength program may be combined.
Time Course Plots Wavelength Program

Changing samples or flows may be examined and displayed Measurements may be programmed for 10 different operator
by making a time course plot. Data may be reprocessed or selected wavelengths with the cycle time and number of
stored, security code protected, with an automatically cycles selected by the operator. A wavelength and cell
assigned recall number. program may be combined.
4
POWERFUL SOFTWARE
SYSTEM FUNCTIONS SAMPLING
DataStream - Fast Data Transfer and Control Cells and Flowcells

Cecil DataStream software provides fast transfer of all data As well as 10mm cells and flowcells, holders are available for
to a PC for incorporation into a spread sheet or mathematical rectangular and cylindrical cells up to 100mm pathlength. A
package of the users choice. Fast and easy to use. holder for solid samples is also available.
Spectrophotometers may also be controlled from the PC.
Self Test and Calibration Automatic Cell Changers

An automatic test procedure, at switch-on, checks all Automatic changers are available for 4, 6 or 8 cells, providing
instrument functions and calibrates wavelength against a rapid sample changing or full programming.
deuterium lamp emission line. Performance is confirmed by
a timed and dated report including instrument serial number.
Method Operation and Storage Sipette Sampling

Up to 30 sets of instrument parameters, including concentration Samples down to 300 µL may be measured in a 10mm cell.
curves may be stored, security code protected. When recalled, Sampling is under control of the instruments microprocessor
instrument parameters are automatically set, avoiding errors. system. Samples may be saved or pumped to waste.
Software Enhancement by E-SEF Micro Sampling

E-SEF - Encoded Software Enhancement Facility - allows Small or valuable samples of 50µL may be measured using a
software modules to be enabled using encoded numbers specially designed cell and a precision holder.
provided by telephone, fax or E-mail. Software requirements
may be tailored, at any time, as required.
Data Storage with Security Ultra-Micro Sampling

Data, including spectra, concentration curves, kinetics and A special precision cell holder is available for ultra-microcells
time course plots may be stored in safe memory, security using as little as 5µL or 10µL of valuable samples. Samples
code protected against tamper or deletion. are loaded into the cell by pipette.
Display Scrolling for Wide Viewing Sample Temperature Control

Use of the display scrolling facility enhances the effective Water and thermoelectric temperature control is available for
screen width to 550mm and enables spectra and data up to single cells and automatic cell changers. Temperature is set
six screen widths to be viewed. from the control panel and displayed on the screen.
Reprocessing Temperature Programming

Scans, time course plots, and reaction curves may all be Temperature programming of samples using a single cell or
reprocessed to any required format on the display screen 4 cell changer is possible using a Thermoelectric controller
and then plotted on an integral or an external printer. with a range of -10˚-110˚C producing temperature
programmes at up to 30˚C per minute.
Multi-Language Operation Batch Sampling

The language of operation may be chosen by the operator Automatic batch sampling for up to 100 samples is available.
from several including: English, French, German, Italian and The pump fits within the sample compartment and volume
Spanish. Menus, prompts, results and printouts are all in the etc is set from the control panel and displayed on screen.
chosen language. Batch number entry is provided.
Performance Validation Dissolution Testing

Software and certified standards enable wavelength Versions of the instruments fitted with an 8 cell changer,
accuracy, Absorbance accuracy, bandwidth etc. to be special piping port and lid, are available for dissolution
validated. A timed and dated report includes the serial testing using Cecil TD software and a PC.
number of the instrument.
5
FAST PROGRAM ANY ASSAY
Fast Program Any Assay
Any quantitative assay may be rapidly input for automatic

computation using the Cecil Assay Program software module.
No programming skill is required by the operator.
Transcription and calculation errors are avoided and validation

is simple as no PC is used. Calculations for a series of Carboxy
Haemoglobin samples is shown below with the formulae used.
Assay Program
Complex assays only require text entry of
any formula with up to 76 characters. For
complex calculations up to 20 such formulae
may be entered and linked for formidable
computing power without a PC.
Assays may use up to 10 wavelengths and 8

cells in any automatic programmed method.
Once entered programs may be stored, as
methods, security protected.
Tabulation of results is automatic and the

ease and speed of use makes Assay program
a very powerful tool.
Multi-Wavelength Assays Flexibility

Absorbance ratios are standard and Simplicity of operation, for even complex tasks, coupled with a
quantitative assays using both two and wide range of software and accessories, makes the Aurius and
three wavelengths are available, including Super Aurius instruments ideal multi-user instruments, and
the methods of Warburg & Christian for excellent for multi-discipline laboratories.
nucleic acids and proteins, shown here.
6
FOR MORE POWER - PERFORMANCE
Super Aurius Performance
The Super Aurius range offers uncompromising
performance and analytical power only available in
much larger and more expensive instruments. With
1.8nm optical bandwidth even closely spaced or narrow
bands may be resolved, as illustrated by the benzene
vapour spectrum shown here.
With the added power of the ‘Quant S’ software
module, scans may be overlayed and differencing,
stripping and band area computation carried out.
Storage for 100 spectra, with full security, is provided.
Derivative spectra are also standard, with multi-cell
Kinetics provided by the ‘Kinetics M’ module.
Derivative Spectra
Spectral information is often masked by overlapping

absorption bands, sloping background absorption, or
the occurrence of a small peak or shoulder on the side
of a broad absorption band. Derivative spectroscopy
may be used to enhance fine detail, reduce the effects
of a sloping background and accurately locate a masked
band for measurement.
Derivatives may be displayed alone or superimposed

on the fundamental, with or without an offset.
Band Quantification
For accurate quantitative work it is often required to

determine the area or height of an absorption band
corrected for background absorption.
A very easy to use programme is provided for this

purpose. The analytical result is displayed on the
screen, with construction lines, enabling the quality of
the chosen parameters to be assessed.
Band quantification may be stored as a method

security protected.
Multi-Cell Kinetics
The Super Aurius Series offers multi-cell Kinetics as
well as single cell Kinetics as available on the Aurius
Series. Plots are displayed on the screen and printed.
Regression analysis is applied to the user selected

portion of the reaction curves (t 1-t 2) and results are
reported in Units/Litre.
Data may be reprocessed and curves stored security
protected.
Water and thermoelectric sample temperature control

are available, also sample stirring.
7
FAST DATA TRANSFER TO PC
DataStream
Cecil DataStream provides rapid transfer of
raw data, or data processed and formatted
by the spectrophotometer, to a PC for
instant display. Scans, measurements,
programmed measurements and
sophisticated analytical procedures may all
be transferred with great ease.
Transferred data may be saved on disk or
incorporated into a spread sheet or maths
pack. Incorporation into Microsoft Excel is
shown here for display, manipulation,
graphical presentation, archiving or report
generation. Full PC control of the
instrument is provided.
Simplicity of operation is a key feature as
any familiar spreadsheet or maths package
- Excel, Lotus 123, Grafit etc - may be used,
avoiding a learning cycle and saving
valuable time. Reports are printed using
any printer supported by Windows.
Advanced Kinetics
For more advanced reaction kinetics data
manipulation, raw kinetics data generated
by the spectrophotometer may be captured
and transferred by DataStream into Grafit
Software.
Using a suitable template the Lineweaver
Burk transformation, shown here, was
automatically plotted and the values of Km
and Vmax extracted. Other transformations
such as Eadie-Hofstee etc. may be used.
Linear, non linear, or polynomial curve
fitting are easily performed together with
linear regression analysis.
Dissolution Assay
Both Aurius and Super Aurius

instrument ranges are offered
equipped for Dissolution Assay
applications when using an
autochanger for up to 8 cells and
come equipped with a piping
port.
Complete systems are offered, as

shown in the picture here,
including dissolution baths
pumps and a choice of
dissolution software.
The Super Aurius should be used

when a narrow 1.8nm bandpass
is required.
8
PERFORMANCE VALIDATION : GLP
Performance Validation Optical Bandwidth

Instrument performance may be validated using a The very accurate method for validating optical
comprehensive software module available on all bandwidth uses the narrow deuterium emission line at
instruments and which may be released by E-SEF. 656.1nm. The width, at half height, of the measured
band due to the emission line measures the actual
Tests are provided for laboratories subject to bandwidth. The band is drawn and the half width
regulatory control or following G.L.P. These include a automatically documented.
range of Pharmacopoeia tests (EP/BP/USP), a selection
of which are shown here.
Absorbance Validation
The Pharmacopoeia (EP/BP) test for absorbance
accuracy is one of the tests provided. The test
measures the values of peaks and valleys in the
absorbance spectrum of a 60mg/L solution of
Potassium Dichromate in a 10mm pathlength cuvette.
The results of such a test run are shown here.
Validation Using Filters
Absorbance and Wavelength validation are most

conveniently carried out using filter sets calibrated by
Cecil Instruments on its reference spectrophotometer
against standards certified by the NPL. Full validation
programmes for these tests are provided.
Straylight
Straylight may be measured using the Pharmacopoeia

(EP/BP) test shown here. This test uses a 1.2% W/V
solution of Potassium Chloride in water, measured
against water in the reference beam. An Absorbance in
excess of 2A must be measured at a wavelength of
200nm.
The ASTM tests at 220nm and 340nm are more widely

used and are provided.
9
UNIVERSAL ACCESSORY RANGE
Micro Sipette Sampling SOFTWARE MODULES
For rapid measurement of samples Assay Program

as small as 300µL with low cross Construction and entry of mathematical
contamination this is the ideal formulae involving measurements of 10
sampling system. wavelengths and 8 cells with automatic
computation of assay results
Without cell handling all
measurements are made in the DataStream
Fast data transfer to PC for use with
same cell, enhancing accuracy and
spread sheets; PC control of instrument
eliminating breakages.
Quant M
The pump, mounted in the sample Multi wavelength assays at 2 or 3
compartment, is controlled by the wavelengths, Warburg and Christian
instrument with control information assays at 2 or 3 wavelengths, wine
and data shown on screen. Fully assay at up to 10 wavelengths
documented printouts are provided.
Quant P
Ultra-Micro Sampling Micro Sampling Protein assay using the methods of
Lowry, Bradford, Biuret and BCA
For the ultimate in small sample For analytical measurements of
Quant S - Super Aurius only
measurement, a special precision minute samples, such as the purity
Quantification of corrected bands,
cell holder is available which of nucleic acid in a biological
difference spectra, spectral stripping,
accommodates a pipette loading sample, or samples prepared with overlayed spectra, spectral storage.
cell with a 10µL chamber volume costly reagents, measurements may
and a 10mm pathlength. A similar be made on samples as small as Program C
cell is also available offering a 50µL, or even less, using a special Cell program for up to 4, 6 or 8 cells
chamber volume of 5µL and 5mm cell and holder. Program W
pathlength. Wavelength program for up to 10
The optical system provides a small
wavelengths and timed interval
image at the sample so that a large
Thermoelectric Temperature proportion of the available energy
measurements at a fixed wavelength
Control passes through the sample, giving Scan - Aurius only
Thermoelectric temperature control accurate, low noise measurements Wavelength scans with peak labelling
is provided by an internal controller. with excellent linearity. and peak tables. Time course plots
The controller is factory fitted and Kinetics
must therefore be specified at the Kinetic measurements with plots,
time of purchase. reprocessing of data
Kinetics M
Temperature control is provided for Multi-cell Kinetics linked with autocell
a single cell, sipette cell or a four changing, and plotting of reaction
cell changer. Provision is also curves
made for work below ambient
temperature. Temperature program- Validation
ming is also provided for thermal Absorbance and Wavelength validation
melt experiments. using certified standards or liquids.
Validation of optical bandwidth, etc.
Automatic Cell Changers Water Thermostatting Sampling Accessories

Four, six and eight cell changers are Water thermostatted holders are A complete range of sampling
available. A long pathlength holder available for single cells, sipette accessories is available, some of
is available for four cells. Water and cells, long pathlength cells and the these are shown and include cell
Thermoelectric temperature controlled four cell changer. A holder with pipe changers, 50µL and 5µL cell holders,
holders may also be used, as shown. port and special lid fitted is shown . film holder and microcell changer.
10
ORDERING
Spectrophotometers are supplied complete with cell AURIUS
holder, power cable, instruction manual and short
form operating instructions. VISIBLE RANGE
SUPER AURIUS 325-1100nm Wavelength range; 4nm Bandpass
325-1100nm Wavelength range; 4nm Bandpass.
1.8nm Bandpass, 190-1100nm wavelength range,
With integral printer
scanning.
1.8nm Bandpass, 190-1100nm wavelength range, UV/VISIBLE RANGE
scanning. With integral printer. CE 2021 Spectrophotometer
190-1100nm Wavelength range, 4nm Bandpass
CE 3021D and CE 3041D Tablet Dissolution
Spectrophotometers CE 2041 Spectrophotometer
Special versions suitable for tablet dissolution 190-1100nm Wavelength range, 4nm Bandpass.
applications, using TD software. With integral printer
System 1 : Cells and Spares Spectrophotometers
Spectrophotometer with two silica cells, spare lamps, Special version suitable for tablet dissolution
spare fuses and dust cover. applications, using TD software
Example: CE2021 system 1 is a CE 2021 with spare System 1
deuterium and tungsten lamps, spare fuses, 2 silica Spectrophotometer, spare lamp(s) spare fuses,
cells and dust cover. 2 cells and dust cover.
Cell Holders Part No. Temperature Control - Water Part No.

Adjustable holder for sipette or single 10mm cell 1010 32 00 Holder for single or sipette cell 1010 33 00
Universal cell holder, 10mm cells upwards 1010 38 00 Holder for 4 cells - for autochanger 2020 36 15
Long pathlength cylindrical cell holder 1010 39 00 Lid and pipe port for water thermostatting 1010 05 00
Holder for solid samples or films 2020 33 00 Temperature Control - Thermoelectric
Ultra-microcell holder : 50µL cells 2020 39 00 Thermoelectric programme controller 7400 30 30
Ultra-microcell holder : 5 and 10µL cells 2021 30 00 Holder for single or sipette cell 2020 31 20
Cell Changers and Holders Holder for 4 cells with autochanger 2021 32 20
4 cell automatic changer (no holder) 2021 34 00 Temperature Programming
4 x 10mm cell holder for auto changer 2010 24 00 Thermoelectric programme controller 7400 30 30
4 x 50mm cell holder for auto changer 2010 36 00 Single 10mm cell holder 2020 31 20
6 cell automatic changer and holder 2021 36 00 Sample Stirring
8 cell tablet dissolution autochanger system 3021 37 00 Electronic controller for sample stirring 7200 31 01
4 microcell manual changer and holder 2020 38 00 Cell holder for sample stirring 2010 32 00
3 x Flowcell changer for water analysis 2020 35 00 Water thermostatted holder for stirring 2010 33 00
Sipette and Batch Sampling 4 cell holder for sample stirring 2010 34 00
Holder for 10mm sipette cell 1010 32 00 Lamps
Holder for sipette cells up to 50mm 2020 37 00 Deuterium lamp with hours indicator 2202 01 42
Batch sampler for 100 samples with pump 2021 82 02 Tungsten halide lamp - in pairs 2303 01 40
Return micro-sipette system no cell or holder 2021 21 00 Printers and Cables
Calibration Filters RS232c cable for 25 pin PC 2021 26 00
Holmium filter in holder 202 01 44 RS232c cable for 9 pin PC 2021 83 00
Didymium filter in holder 303 01 30 Parallel printer connection cable 8000 71 00
Set of 2 certified wavelength filters in holders 303 40 00 Dot matrix printer 8000 70 01
Set of 4 certified Absorbance filters in holders 594 44 00 Colour inkjet printer 8000 72 01
Set of 6 certified Absorbance filters in holders 594 66 00 Print rolls and ribbon for integral printer 2020 03 20
11
SPECIFICATIONS HIGHLIGHTS
Monochromator Littrow, 1200 lines/mm holographic grating
Optical Bandwidth 4nm - Aurius; 1.8nm Super Aurius BENEFITS
Wavelength Range 190-1100nm; 325-1100nm visible versions
Wavelength Accuracy Better than ±1nm High Performance Scans
Wavelength Reproducibility 0.1nm
Straylight Typically less than 0.01% at 220 and 340nm
Derivative Spectra
Display Screen - LCD High resolution backlit with contrast control
Display Scrolling Provides 6 screen widths for viewing plots etc. Time Plots
Autozero Automatic by press button
Photometric Ranges Digital display of -0.3-3A, 0-200%T, 0-9999C Assay Formula Program
Photometric Accuracy ±0.005A or 1% whichever is greater
Photometric Noise Less than ±0.00004A (500nm RMS)
Stability Better than 0.001A/hour at constant temp. DataStream - Fast Data to PC
Multi-Wavelength Analysis 2 and 3 wavelength methods
Assay Program Enter multi-wavelength formula for automatic Display Scrolling - 6 Screens Wide
computation and formatting of any assay
Curve Fitting and Editing Linear, quadratic or cubic; up to 30 standards
Cell and Wavelength Programming Up to 8 cells and 10 wavelengths combined E-SEF Software Call-up
Method Storage Up to 30 methods stored in protected memory
Absorbance Ratioing 260/280nm or any two selected wavelengths Integral Printer Versions
Peak Seek Automatic search, baseline corrected
Reaction Kinetics Tabulated results, reaction plots, reprocessing
Method Storage
Batch Sampling 100 samples, internal control, screen display
Real Time Clock Timed and dated reports
Computer/printer interfaces Bi-directional serial RS232 and parallel ports Cell and Wavelength Programs
Sample Temperature Displayed and set from keyboard
Size 480 x 340 x 205mm Kinetics, Single/Multi-cell
Weight 16.8kg Visible; 18.6kg UV; + 0.9kg with printer
Power Requirements 110-250V, 50/60Hz; 120W/170W UV versions
Multi-Wavelength Assays
UV Lamp One Year Guarantee

SUPER AURIUS ADDITIONAL SPECIFICATION
Optical Bandwidth 1.8nm
Wavelength Scanning and Range 1-4000nm/min; 190-1100nm Compact - Only 48cm Wide
Wavelength Scale Expansion Selectable by keyboard entry 1-100nm/cm
Scan Speed Selectable by keyboard entry 1-4000nm/min
Photometric Plotting Ranges Keyboard entry of limits; or automatic
optimum PERFORMANCE
Baseline Flatness Better than ±0.002A (300nm/min) most of
range
Overlayed Spectra Up to 6 Spectra and derivatives with or Optical Bandwidth 1.8nm
without offsets
Spectral Reprocessing Spectra manipulated and replotted over any Straylight 0.01%
range
Dynamic Scan Recall Instant, automatic recall of last 8 scans
Spectral Storage Up to 100 spectra may be stored security Wavelength Precision 0.1nm
code protected
Derivative Spectra 1st and 2nd derivatives
Multi-cell Kinetics Up to 4 or 6 cells, with with plots and Stability ±0.001 A/hour
reprocessing
Band Quantification Automatic band area and height correction Accurate to 3A
Time Course Plotting Plots may be reprocessed and stored
Cecil Instruments policy is one of continuous development. We therefore reserve the right to change specification without notice. Full Validation Procedures
CECIL INSTRUMENTS LIMITED MILTON TECHNICAL CENTRE

CAMBRIDGE CB24 6AZ ENGLAND ISO 9000
TEL: 01223 420821 FAX: 01223 420475 CERTIFIED COMPANY
E-MAIL: info@cecilinstruments.com WEBSITE: www.cecilinstruments.com
January 2016
™
DNA / RNA
ANALYSER
™
DEDICATED
TED TO DNA / RNA
Simplicity of Operation
Insert the reference, press ZERO, then insert the
sample and press RUN to obtain a fully documented
report complete with header, sample number and
space for the operators name etc. Operation could not
be more simple.
Operators may create and store up to 30 of their own
methods with the method storage option.
Automatic Wavelength Selection

All the fully programmed methods are selected from a
menu and displayed with headers, prompts and details
on the large display screen on which information may
be scrolled for viewing.
All wavelengths for any given assay are automatically
selected and measured. Results are displayed on the
screen and a timed and dated printout is available on
an external printer, or the internal printer of the
Dedicated to Molecular Biology CE2302.
The GeneQuest™ Analyser is a spectrophotometer
dedicated to the requirements of the Molecular Enhanced Performance
Biology Laboratory. Fully programmed methods The excellent accuracy of the GeneQuest™ for normal
include those for RNA, ds DNA, ss DNA, samples is further enhanced for poorly transmitting
oligonucleotide primer, proteins, bacterial growth and samples by an automatic integration process initiated
cell culture measurements and molecular calculations, in response to an alert message.
including theoretical thermal melt.
Validation
Ultra Micro-Samples To fulfil legislative requirements and GLP, onboard
Measurements are possible for a wide range of sample software allows for performance validation using filter
volumes, from 2000µL to 5µL using an appropriately sets calibrated by Cecil Instruments and supplied with
selected cell in the ultra microcell holder fitted as calibration certificates traceable to NPL standards.
standard in the GeneQuest™. Methods for liquid samples are also provided.
STANDARD SOFTWARE
● A260/A280 Ratio ● ss DNA concentration
● A260/A280 with background correction at 320nm ● ds DNA concentration
● A260/A230 Ratio ● RNA concentration
● A260/A230 with background correction at 320nm ● Direct UV method for protein at 280nm
SAMPLE CELL SELECTION Volume Pathlength Cell Description Cell Type Part Number
(µL) (mm)
2000 10 Standard A 202 07 26
A range of UV transmitting silica cells is
500 10 Semi-micro, thick base B 202 07 57
available. The listing given here enables the
250 10 Micro, thick base C 202 07 38
cell appropriate to the sample volume to be
70 10 Ultra micro D 202 07 15
chosen. All cells except the standard must be
50 10 Ultra micro E 2020 07 01
of the self masking type.
use holder 2020 39 00
10 10 Ultra micro, pipette filling F 202 07 64
Using a 10mm pathlength cell an absorbance A
1000 5 Standard - use spacer 202 07 25
value of 1 is estimated for a concentration of F
5 5 Ultra micro, pipette filling 202 07 63
50µL/mL of ds DNA
A B C D E F
2
AND MOLECULAR BIOLOGY
Assays Displayed on Screen

Shown here are three screen displays of
typical assays, for protein by the direct UV
method, ratios at 260/280nm and a theoretical
melt Tm calculation.
Printout of Assays
The printouts shown here are for typical
assays using the Bradford method at 595nm,
the determination of ds DNA, and the results
of the Kalb Bernlohr determination of nucleic
acids and proteins.
A plot of any curve or straight line fitted to a

series of standards is always available to
verify the fit.
The printout on the left of this page shows a

list of all the available methods offered by the
GeneQuest™.
3
SPECIFICATION
™
OPTIONAL SOFTWARE - E-SEF Enabled ORDERING

SM1 Nucleic Acid and Protein - more advanced calculations:
BENEFITS
Warburg and Christian
DNA 260/280
DNA 260/280, 320 ACCESSORIES
Full screen display of menus,AND S
Protein 260/230 prompts and results
Protein 260/230, 320
Kalb and Bernlohr SPECIFICATION

Software options password
DNA 260/280 enabled at any time by E-SEF
DNA 260/280, 320
Protein
Protein
260/230
260/230, 320
OPTIONAL SOFTWAR
Storage for 30 user methods,
password protected
SM2 Molecular calculations
Molecular concentrations
Recovery of oligonucleotides Warning of poor sample
Phosphate concentration transmission
Protein purity
Theoretical thermal melt
Signal enhancement for poor
SM3 Protein Assay - Bradford 595nm sample transmission
ORDERING
SM4 Bacterial Growth
Optical density at 600nm Plot of curve fitted to standards
SM5 Validation: Wavelength, Absorbance, Bandwidth etc

BENEFITS Full UV range available to create
SM6 Method Storage for 30 methods security protected methods
ORDERING
ACCESSORIES AND SPARES
Cell holder for 50µL cells - type E 2020 39 00
Printer connection cable 8000 71 00
BENEFITS
SPECIFICATION
Dot matrix printer 8000 70 01
Spare deuterium lamp 2202 01 42
2 Certified wavelength filters with certificate 303 40 00
ACCESSORIES
OPTIONAL AND SPARES
SOFTWARE - E-SEF Enabled
4 Certified Absorbance filters with certificate 594 99 00
Dust cover 2020 01 23
SPECIFICATION ORDERING
Wavelength Range 190-370nm, 595 and 600nm
Wavelength Accuracy ±1nm
OPTIONAL SOFTWARE - E-SEF Enabled
Wavelength Reproducibility ±0.1nm
Each GeneQuest analyser is
BENEFITS
supplied complete with an ultra
Wavelength Calibration Automatic at switch on
microcell holder, power cable,
Wavelength Selection Automatic for assay selected
operator’s manual and short
Monochromator
Optical Bandwidth
Littrow 1200 lines per mm holographic grating
4nm
ACCESSORIES AND S
form operating instructions.
Straylight Less than 0.02% at 280nm (Acetone)
Photometric Range -0.3 - 3 Absorbance CE 2301 Analyser
Photometric Linearity ±1% or ±0.005, whichever is greater SPECIFICATION
190-370nm, 595 and 600nm
4nm optical bandwidth
Stability Better than 0.001A/hour at 240nm constant T.
Light Source Deuterium lamp with life indicator CE 2302 Analyser
Printer Output Centronics parallel port OPTIONAL
190-370nm, 595 and SOFTWARE
600nm
Power Requirements 110-240V, 50/60Hz, 100W 4nm optical bandwidth
Size and Weight 480 x 340 x 205, 17.6Kg, (18.5kg printer version) With integral printer
GeneQuest™ is a registered Trade Mark of Cecil Instruments Ltd.
ISO 9000
TELEPHONE: 01223 420821 FAX: 01223 420475
CERTIFIED COMPANY
E-MAIL: info@cecil-instruments.co.uk WEB SITE: www.cecil-instruments.co.uk
4
TM TM
ANALYSIS
BIO ANAL YSIS
BioQuest TM
Compact design, 4nm optical bandwidth. Fully programmed for
bioanalysis, scanning, integral printer version.
BioAquariusTM
Symmetrical double beam design, 1.8nm optical bandwidth. Fully
programmed for bioanalysis. A very capable general purpose scanning
double beam spectrophotometer.
Bio Spectrophotometers Reaction Kinetics

Specially pre-programmed for bioanalysis and Both single and up to 6 cell multi-cell Kinetics are
molecular biology, the BioQuest and BioAquarius also readily carried out. Water or thermoelectric
have all the normal functions of UV visible temperature control is available also sample stirring.
spectrophotometers, scanning, time course plots,
Reaction curves are displayed on screen and
single wavelength measurements in Absorbance,
regression analysis applied to the user selected part of
Concentration or Transmittance, cell and wavelength
the curves to calculate results.
programming, method storage etc.
Advanced Kinetics is possible providing such plots as
Protein Analysis Michaelis Menton and Lineweaver Burk etc., from
Preprogrammed methods for all the normal protein which Km and Vmax are automatically calculated.
analyses are provided, these include the following
methods:– Small Sample Measurements
A return Micro Sipette system is available which uses
Bradford Biuret a 75µL cell, measurements on samples of 300µL or less
Lowry (low sens.) BCA may be made with low levels of cross contamination.
Lowry (high sens.) Direct UV A special holder accommodates cells of 50µL for
measuring valuable small samples. These cells may be
Assays may also be carried out using the Warburg and used in both sample and reference beams in the
Christian 2 or 3 wavelength methods and the Kalb and BioAquarius instrument.
Bernlohr procedures, all of which are preprogrammed.
For the ultimate in small sampling a nano cell or special pipette
loaded 5µL cell with 5mm pathlength are available.
Nucleic Acid Assays
Concentration and purity may be determined by the Experimental Thermal Melt (Tm)
methods of Warburg and Christian, Kalb and Bernlohr
Thermoelectric temperature programming is available
or using user selected parameters.
for thermal melt (Tm) determinations.
DNA, ssDNA, dsDNA, RNA and oligonucleotides may A fully annotated plot of Absorbance against
be quantified and examined for purity using one temperature is displayed. Data may be reprocessed,
wavelength, or two or three wavelength ratio methods. derivatised, overlayed, stored or transferred to a PC.
Molecular concentration may be determined for Oligo Method Generation and Storage
Samples and theoretical melting points for DNA All preprogrammed methods may be modified by the
samples calculated using the nearest neighbour user to meet their requirements. Any modified method
interaction model. may then be stored as an additional method in code
protected memory.
For more accurate measurements and for DataStream - Data to PC
measurements where Beer’s law is not obeyed a The DataStream system allows all data to be exported
straight line quadratic or cubic curve may be fitted to rapidly and with great ease, to a PC for incorporation
up to 30 standards or replicate standards. Editing of into a spreadsheet or mathematical package of the
suspect standards may be carried out and the final users choice - Excel, Grafit etc - for report generation
curve stored in code protected memory for future use. and mathematical manipulation.
2
MOLECULAR BIOLOGY
Program Any Assay Application Example
The Cecil Assay program software module enables users to A typical experiment is illustrated here.
mathematically formulate any assay for measurement at up to 10 The scans of protein, DNA and a sample
wavelengths. Rapid text entry of any formula and setting a mixture are shown overlayed over the
wavelength program is all that is required for automatic range 230-330nm.
measurement of any sample. A cell program may be incorporated for
multiple automatic sample measurements. Up to 20 formulae, each An automatic calculation of the ratios
with up to 76 characters, may be combined for the most complex of and the DNA purity of the sample was
assays. made using the pre-programmed three
Never before has programming assays for automatic measurement wavelength ratio method. The results
and computation been so easy. were automatically tabulated as shown.
BioQuestTM BioAquariusTM BioQuestTM BioAquariusTM
Cell Holders Temperature Control - Water
Micro sipette 10mm cell holder 1010 32 00 503 26 00 Single or Sipette cell holder 1010 33 00 503 36 00
Adjustable holder for micro cell 6600 57 00 4 cell holder 2020 36 15 202 36 16
50µl ultra microcell holder 2020 39 00 8020 39 00 2 x 4 cell holder, sample and ref. 202 36 15
Rectangular cells up to 100mm 1010 38 00 595 27 00 6 cell holder 5500 36 15
Cylindrical cells up to 100mm 1010 39 00 595 26 00 Thermoelectric Control
Cell Changers Thermoelectric Controller CE 2024 CE 2024
4 cell auto changer, no holder 2021 34 00 9070 31 00 Single or sipette cell holder 2021 31 20 8020 56 00
Holder for 4 x 10mm cells 2010 24 00 2010 24 00 4 cell holder 2021 32 20 9070 57 00
6 cell auto changer, no holder 9070 34 00 2 x 4 cell holder 9070 58 00
6 cell auto changer and holder 2021 36 00 6 cell holder 9070 59 00
Holder for 6 x 10mm cells 5500 33 00 Gel Scanning
8 cell auto changer with holder 3021 37 00 9070 32 00 Gel scanner - no trough 9070 39 00
4 position microcell changer 2020 38 00 505 38 00 Gel trough - 5 x 100mm, silica 570 07 02
Sipette and Batch Sampling Printers and Cables
Sipette system, no holder or cell 2021 21 00 9070 21 00 RS232 cable for 25 pin PC 2021 26 00 2021 26 00
10mm sipette cell 202 07 02 202 07 02 RS232 cable for 9 pin PC 2021 83 00 2021 83 00
40 Batch sampler with pump 2021 82 00 2021 82 00 Printer connection cable 8000 71 00 8000 71 00
100 Batch sampler with pump 2021 82 02 2021 82 02 Dot matrix printer 8000 70 01 8000 70 01
Lamps Colour ink jet printer 8000 72 01 8000 72 01
Deuterium lamp with indicator 2202 01 42 2202 01 42 Certified Calibration Filter Sets
Tungsten halide lamp - in pairs 2303 01 40 2303 01 40 2 Wavelength filters 303 40 00 303 40 00
Sample Stirring 4 Absorbance filters 594 44 00 594 44 00
Electronic stirrer 2010 31 00 2010 31 00 6 Absorbance filters 594 66 00 594 66 00
Cell holder 10mm cell 2010 32 00 579 02 00 2 Wavelength and 6 Abs. filters 594 77 00 594 77 00
3
TM
ORDERING
Spectrophotometers are supplied
complete with cell holder, power
SPECIFICATIONS cable, operators manual and short
BioQuestTM BioAquariusTM form operating instructions.
Optical System Single Beam Symmetrical Double Beam BioQuestTM

Monochromator Littrow, 1200 L/mm grating Czerny Turner 1200 L/mm grating
Wavelength Range 190 - 1100nm 190 - 1100nm CE 2501 Spectrophotometer
Wavelength Accuracy Better than 1 nm Better than 0.5nm 190-1100nm wavelength range
4nm Optical bandwidth.
Wavelength Reproducibility Better than 0.1nm
Scan Speeds 1-4000nm/min CE 2502 Spectrophotometer
Photometric Range -0.3 - 3A, 0-200% T and C
190-1100nm wavelength range
Photometric Noise Better than 0.00004A at 1A, 500nm RMS
4nm Optical bandwidth with
Photometric Accuracy Better than 0.005A at 1A Better than 0.004A at 1A integral printer.
Baseline Flatness Better than ±0.002A p.t.p. Better than ± 0.001A p.t.p.
Method Storage 30 methods security code protected BioAquariusTM

Spectral Storage 100 spectra, code protected CE 7450 Spectrophotometer
Derivative Spectra 1st to 4th 190-1100nm wavelength range
Overlayed Spectra Up to 6 1.8nm Optical bandwidth.
Concentration Curve Fitting Linear, quadratic and cubic curves, to 30 concentration
standards or replicates
Display Screen With Scrolling Large LCD with 6 screen widths of scrolling for wide view SOFTWARE MODULES
Program C
Stability Better than 0.001A/hr Better than 0.0001A/hr
Cell program for 4, 6 or 8 cells,
Computer Interface Bi-directional serial RS232 and USB ports may be combined with Wavelength
Program.
Size and Weight 480 x 340 x 205; 18.6 Kg 635 x 410 x 210; 33Kg
19.5 Wavelength Program
Up to 10 wavelengths may be
PRE-PROGRAMMED BIO-ASSAYS programmed and the program
Protein may be combined with a Cell
Lowry Low Sensitivity Program.
Lowry High Sensitivity
Bradford Quant S
Biuret Quantification of corrected bands,
BCA difference spectra, spectral
Direct UV stripping.
Warburg and Christian 260/280
Warburg and Christian 260/280 : 320 Background Kinetics M
Kalb and Bernlohr 260/230 Multi-cell Kinetics for up to 4 or 6
Kalb and Bernlohr 260/230 : 320 Background cells.
DNA
Ratio at 260/280 Validation
Ratio at 260/280 : 320 Background Validation of Absorbance,
Ratio at 260/230
wavelength, bandwidth, straylight
Ratio at 260/230 : 320 Background
etc. using liquid samples or
Warburg and Christian 260/280
Warburg and Christian 260/280 : 320 Background certified standards.
Kalb and Bernlohr 260/230
Kalb and Bernlohr 260/230 : 320 Background DataStream
ss D N A Fast data transfer to PC for use
ds D N A with Excel or other spread sheets.
RNA
Thermal melt Tm Assay Program
Molecular concentration Construction and entry of
Percent recovery mathematical formulae involving
Phosphate concentration measurements at multiple
Ratio at two user selected wavelengths wavelengths with automatic
Difference at two user selected wavelengths computation of assay results.
Multi-Wavelength Assay using user selected wavelengths
Concentration Curve Fit : linear, quadratic, cubic to 30 standards and replicates
All user specific assays may be stored as protected methods
ISO 9001 : 2008
TELEPHONE: 01223 420821 FAX: 01223 420475
CERTIFIED COMPANY
4
UV-VISIBLE SPECTROPHOTOMETERS
The Choice
Aurius Series Super Aurius Series
With an optical bandwidth of 4nm and superb stability, The Super Aurius instruments are unique for their
accuracy and precision these very easy to use very high performance scanning, with derivatives,
instruments are ideal general purpose overlay, extended analytical power and multi-cell
spectrophotometers. Kinetics. They cover the full 190-1100nm wavelength
Excellent for routine and quality control laboratories, range with a narrow 1.8nm optical bandwidth.
they offer a wide range of techniques with wavelength With straylight of 0.01%, wavelength precision of
scanning as an option. Both UV and Visible versions 0.1nm and baseline stability better than ±0.001A/hr,
are available with full validation procedures. the most demanding analysis may be undertaken.
Performance with Convenience Ease of Operation

The ease of operation makes even complex tasks The large screen displays all menus and prompts. All
simple. An exceptional range of software and curves, scans and data are screen displayed and may
accessories makes the Aurius and Super Aurius be reprocessed before printing.
instruments superb multi-user instruments, ideal for
multi-discipline laboratories. Software Enhancement by E-SEF
E-SEF - Encoded Software Enhancement Facility - is an
Absorbance Accuracy important new Cecil design feature. Optional software
The low straylight of the instruments, less than 0.01%, modules are enabled by entering correctly allocated
allows a measurement of 3 Absorbances to be made encoded numbers. These numbers can be provided by
with an error close to 1%. This should be compared telephone, fax or E-Mail. Exact software requirements
with errors of 26%, 10% and 6% which result from may therefore be tailored after instrument purchase
straylight of 0.5%, 0.1% and 0.05% respectively for the without incurring delay before use.
same measurement.
Integral Printer
Six Screen width Viewing The integral printer versions provide hard copy of
All scans, curves and data produced by the spectra, curves and time course plots, as well as timed
spectrophotometers may be scrolled across the screen and dated reports of all experiments. An external,
providing a very wide 6 screen width - 550mm - for Epson compatible, printer may be used providing large
viewing of extended displays. high quality plots and documentation of assays.
2
SIX SCREEN WIDTH VIEWING
Accurate Quantification
For optimum accuracy when measuring concentration, a calibration
line may be fitted to a series of up to 30 standards or replicates.
Accuracy is improved over using a single known concentration.

When concentration is not linearly related
to Absorbance a quadratic or cubic curve
may be fitted to up to 30 standards or
replicates, with or without an intercept. A
straight line may be fitted when a linear
relationship exists. Curves are displayed
with data for each standard documented.
Editing of Data
Suspect standards revealed by the initial fit
may be deleted or replaced for a new fit. Up
to 30 curves may be stored security
protected.
Wavelength Scanning
Wavelength scanning from 190-1100nm is standard
on Super Aurius instruments with a narrow 1.8nm
optical bandwidth. For the Aurius range scanning
software is optional with a 4nm optical bandwidth.
Scans at up to 4000nm/min are displayed with

peaks and valleys tabulated and scans of up to 6
screen widths may be viewed by scrolling.
Spectra may be printed on either the integral
printer, as shown below, or an external printer.
The Super Aurius range offers in addition:

Scanning, derivitatives and time plots as standard.
Spectral storage, overlay, band quantification,
stripping and differencing are also available.

A cell program software module enables up to 4, 6 A combined cell and wavelength program may be run for
or 8 cells to be programmed with the appropriate up to 8 cells and 10 wavelengths.
cell changer. A cell and wavelength program may be used for
Programming of up to 10 wavelengths is also calculations using complex formulae entered using the
provided by the wavelength program software Assay program. Results are automatically formatted and
module. printed.
3
FAST : EASY TO USE :
NOTE: This column applies only to Super Aurius.
Scan is an option on the Aurius range.
Wavelength Scan Assay Program

Scans at up to 4000nm/min are presented on screen or Formulae for measurements at up to 10 wavelengths and 8
printed with all peaks annotated with wavelength and cells may be entered rapidly. Programming and formatting of
Absorbance. Tables are also presented listing all peaks and results is automatic at entry without user intervention. Multi-
troughs with values. sample assays may be stored as methods.
Derivative Spectra Concentration Measurement

Derivative spectra are readily produced, with selectable In the concentration measurement mode samples may be
smoothing, either alone or superimposed on the measured either by using a calibration standard, or entering
fundamental, with or without an offset. Scaling is automatic, a known factor relating concentration to Absorbance.
with all peaks and troughs quantified.
Overlayed Spectra Curve and Line Fitting

Up to six scans, including stored scans, may be overlayed, A straight line, quadratic or cubic curve may be fitted to up to
with or without offsets for convenient comparisons or further 30 standards, with or without an intercept at the origin.
manipulation. Standards may be deleted and replaced and a new curve fitted.

The last eight scans are automatically retained for recall by Single and multi-cell kinetic measurements are possible with
selection from a list and may be reprocessed, overlayed or plots and data displayed on screen. Data may be reprocessed
transferred to protected memory for future use. and curves stored, security code protected against tamper or
deletion.
New scans or scans from store may be presented on screen Assays are provided using either two or three wavelengths.
and the difference spectra produced. The scans may be A wine colour assay is provided which may use up to ten
normalised enabling spectral stripping to reveal impurities or different wavelengths. Multi-wavelength computations are
additives. possible up to 10 wavelengths.
Band Area Height Computation DNA and Protein Assays

Band areas with correction for sloping background Protein analyses may be made using either the 2 or 3
absorption are readily quantified, with all details and wavelength methods of Warburg and Christian. Also
construction displayed. Band areas may also be computed programmed are techniques and analytical procedures for
for derivative spectra to facilitate difficult quantification. assays by Lowry, Bradford, Biuret and BCA methods.
Scan Storage Ratio and Difference

Spectra may be stored in safe memory for future recall. Each Absorbance ratioing at 280/260nm, or two user selected
spectrum is automatically allocated a recall number, when wavelengths, is provided for DNA purity assays. The scaled
stored, and may be security code protected against tamper difference of Absorbance values at two wavelengths is also
or deletion. provided.

Scans, automatic, single or repeat may be made of a cell Using the various cell changers, measurements may be
program of up to 4, 6 or 8 cells. A programmed sequence of made on up to 4, 6 or 8 cells at a single wavelength with cycle
scans may also be made of a single sample. time and number of cycles entered by the operator. A cell
and wavelength program may be combined.

Changing samples or flows may be examined and displayed Measurements may be programmed for 10 different operator
by making a time course plot. Data may be reprocessed or selected wavelengths with the cycle time and number of
stored, security code protected, with an automatically cycles selected by the operator. A wavelength and cell
assigned recall number. program may be combined.
4
POWERFUL SOFTWARE
DataStream - Fast Data Transfer and Control Cells and Flowcells

Cecil DataStream software provides fast transfer of all data As well as 10mm cells and flowcells, holders are available for
to a PC for incorporation into a spread sheet or mathematical rectangular and cylindrical cells up to 100mm pathlength. A
package of the users choice. Fast and easy to use. holder for solid samples is also available.
Spectrophotometers may also be controlled from the PC.
Self Test and Calibration Automatic Cell Changers

An automatic test procedure, at switch-on, checks all Automatic changers are available for 4, 6 or 8 cells, providing
instrument functions and calibrates wavelength against a rapid sample changing or full programming.
deuterium lamp emission line. Performance is confirmed by
a timed and dated report including instrument serial number.
Method Operation and Storage Sipette Sampling

Up to 30 sets of instrument parameters, including concentration Samples down to 300 µL may be measured in a 10mm cell.
curves may be stored, security code protected. When recalled, Sampling is under control of the instruments microprocessor
instrument parameters are automatically set, avoiding errors. system. Samples may be saved or pumped to waste.
Software Enhancement by E-SEF Micro Sampling

E-SEF - Encoded Software Enhancement Facility - allows Small or valuable samples of 50µL may be measured using a
software modules to be enabled using encoded numbers specially designed cell and a precision holder.
provided by telephone, fax or E-mail. Software requirements
may be tailored, at any time, as required.
Data Storage with Security Ultra-Micro Sampling

Data, including spectra, concentration curves, kinetics and A special precision cell holder is available for ultra-microcells
time course plots may be stored in safe memory, security using as little as 5µL or 10µL of valuable samples. Samples
code protected against tamper or deletion. are loaded into the cell by pipette.
Display Scrolling for Wide Viewing Sample Temperature Control

Use of the display scrolling facility enhances the effective Water and thermoelectric temperature control is available for
screen width to 550mm and enables spectra and data up to single cells and automatic cell changers. Temperature is set
six screen widths to be viewed. from the control panel and displayed on the screen.
Reprocessing Temperature Programming

Scans, time course plots, and reaction curves may all be Temperature programming of samples using a single cell or
reprocessed to any required format on the display screen 4 cell changer is possible using a Thermoelectric controller
and then plotted on an integral or an external printer. with a range of -10˚-110˚C producing temperature
programmes at up to 30˚C per minute.
Multi-Language Operation Batch Sampling

The language of operation may be chosen by the operator Automatic batch sampling for up to 100 samples is available.
from several including: English, French, German, Italian and The pump fits within the sample compartment and volume
Spanish. Menus, prompts, results and printouts are all in the etc is set from the control panel and displayed on screen.
chosen language. Batch number entry is provided.
Performance Validation Dissolution Testing

Software and certified standards enable wavelength Versions of the instruments fitted with an 8 cell changer,
accuracy, Absorbance accuracy, bandwidth etc. to be special piping port and lid, are available for dissolution
validated. A timed and dated report includes the serial testing using Cecil TD software and a PC.
number of the instrument.
5
FAST PROGRAM ANY ASSAY
Fast Program Any Assay
Any quantitative assay may be rapidly input for automatic

computation using the Cecil Assay Program software module.
No programming skill is required by the operator.
Transcription and calculation errors are avoided and validation

is simple as no PC is used. Calculations for a series of Carboxy
Haemoglobin samples is shown below with the formulae used.
Assay Program
Complex assays only require text entry of
any formula with up to 76 characters. For
complex calculations up to 20 such formulae
may be entered and linked for formidable
computing power without a PC.
Assays may use up to 10 wavelengths and 8

cells in any automatic programmed method.
Once entered programs may be stored, as
methods, security protected.
Tabulation of results is automatic and the

ease and speed of use makes Assay program
a very powerful tool.
Multi-Wavelength Assays Flexibility

Absorbance ratios are standard and Simplicity of operation, for even complex tasks, coupled with a
quantitative assays using both two and wide range of software and accessories, makes the Aurius and
three wavelengths are available, including Super Aurius instruments ideal multi-user instruments, and
the methods of Warburg & Christian for excellent for multi-discipline laboratories.
nucleic acids and proteins, shown here.
6
FOR MORE POWER - PERFORMANCE
Super Aurius Performance
The Super Aurius range offers uncompromising
performance and analytical power only available in
much larger and more expensive instruments. With
1.8nm optical bandwidth even closely spaced or narrow
bands may be resolved, as illustrated by the benzene
vapour spectrum shown here.
With the added power of the ‘Quant S’ software
module, scans may be overlayed and differencing,
stripping and band area computation carried out.
Storage for 100 spectra, with full security, is provided.
Derivative spectra are also standard, with multi-cell
Kinetics provided by the ‘Kinetics M’ module.
Derivative Spectra
Spectral information is often masked by overlapping

absorption bands, sloping background absorption, or
the occurrence of a small peak or shoulder on the side
of a broad absorption band. Derivative spectroscopy
may be used to enhance fine detail, reduce the effects
of a sloping background and accurately locate a masked
band for measurement.
Derivatives may be displayed alone or superimposed

on the fundamental, with or without an offset.
Band Quantification
For accurate quantitative work it is often required to

determine the area or height of an absorption band
corrected for background absorption.
A very easy to use programme is provided for this

purpose. The analytical result is displayed on the
screen, with construction lines, enabling the quality of
the chosen parameters to be assessed.
Band quantification may be stored as a method

security protected.
Multi-Cell Kinetics
The Super Aurius Series offers multi-cell Kinetics as
well as single cell Kinetics as available on the Aurius
Series. Plots are displayed on the screen and printed.
Regression analysis is applied to the user selected

portion of the reaction curves (t 1-t 2) and results are
reported in Units/Litre.
Data may be reprocessed and curves stored security
protected.
Water and thermoelectric sample temperature control

are available, also sample stirring.
7
FAST DATA TRANSFER TO PC
DataStream
Cecil DataStream provides rapid transfer of
raw data, or data processed and formatted
by the spectrophotometer, to a PC for
instant display. Scans, measurements,
programmed measurements and
sophisticated analytical procedures may all
be transferred with great ease.
Transferred data may be saved on disk or
incorporated into a spread sheet or maths
pack. Incorporation into Microsoft Excel is
shown here for display, manipulation,
graphical presentation, archiving or report
generation. Full PC control of the
instrument is provided.
Simplicity of operation is a key feature as
any familiar spreadsheet or maths package
- Excel, Lotus 123, Grafit etc - may be used,
avoiding a learning cycle and saving
valuable time. Reports are printed using
any printer supported by Windows.
Advanced Kinetics
For more advanced reaction kinetics data
manipulation, raw kinetics data generated
by the spectrophotometer may be captured
and transferred by DataStream into Grafit
Software.
Using a suitable template the Lineweaver
Burk transformation, shown here, was
automatically plotted and the values of Km
and Vmax extracted. Other transformations
such as Eadie-Hofstee etc. may be used.
Linear, non linear, or polynomial curve
fitting are easily performed together with
linear regression analysis.
Dissolution Assay
Both Aurius and Super Aurius

instrument ranges are offered
equipped for Dissolution Assay
applications when using an
autochanger for up to 8 cells and
come equipped with a piping
port.
Complete systems are offered, as

shown in the picture here,
including dissolution baths
pumps and a choice of
dissolution software.
The Super Aurius should be used

when a narrow 1.8nm bandpass
is required.
8
PERFORMANCE VALIDATION : GLP
Performance Validation Optical Bandwidth

Instrument performance may be validated using a The very accurate method for validating optical
comprehensive software module available on all bandwidth uses the narrow deuterium emission line at
instruments and which may be released by E-SEF. 656.1nm. The width, at half height, of the measured
band due to the emission line measures the actual
Tests are provided for laboratories subject to bandwidth. The band is drawn and the half width
regulatory control or following G.L.P. These include a automatically documented.
range of Pharmacopoeia tests (EP/BP/USP), a selection
of which are shown here.
Absorbance Validation
The Pharmacopoeia (EP/BP) test for absorbance
accuracy is one of the tests provided. The test
measures the values of peaks and valleys in the
absorbance spectrum of a 60mg/L solution of
Potassium Dichromate in a 10mm pathlength cuvette.
The results of such a test run are shown here.
Absorbance and Wavelength validation are most

conveniently carried out using filter sets calibrated by
Cecil Instruments on its reference spectrophotometer
against standards certified by the NPL. Full validation
programmes for these tests are provided.
Straylight
Straylight may be measured using the Pharmacopoeia

(EP/BP) test shown here. This test uses a 1.2% W/V
solution of Potassium Chloride in water, measured
against water in the reference beam. An Absorbance in
excess of 2A must be measured at a wavelength of
200nm.
The ASTM tests at 220nm and 340nm are more widely

used and are provided.
9
UNIVERSAL ACCESSORY RANGE
Micro Sipette Sampling SOFTWARE MODULES
For rapid measurement of samples Assay Program

as small as 300µL with low cross Construction and entry of mathematical
contamination this is the ideal formulae involving measurements of 10
sampling system. wavelengths and 8 cells with automatic
computation of assay results
Without cell handling all
measurements are made in the DataStream
Fast data transfer to PC for use with
same cell, enhancing accuracy and
spread sheets; PC control of instrument
eliminating breakages.
Quant M
The pump, mounted in the sample Multi wavelength assays at 2 or 3
compartment, is controlled by the wavelengths, Warburg and Christian
instrument with control information assays at 2 or 3 wavelengths, wine
and data shown on screen. Fully assay at up to 10 wavelengths
documented printouts are provided.
Quant P
Ultra-Micro Sampling Micro Sampling Protein assay using the methods of
Lowry, Bradford, Biuret and BCA
For the ultimate in small sample For analytical measurements of
Quant S - Super Aurius only
measurement, a special precision minute samples, such as the purity
Quantification of corrected bands,
cell holder is available which of nucleic acid in a biological
accommodates a pipette loading sample, or samples prepared with overlayed spectra, spectral storage.
cell with a 10µL chamber volume costly reagents, measurements may
and a 10mm pathlength. A similar be made on samples as small as Program C
cell is also available offering a 50µL, or even less, using a special Cell program for up to 4, 6 or 8 cells
chamber volume of 5µL and 5mm cell and holder. Program W
pathlength. Wavelength program for up to 10
The optical system provides a small
image at the sample so that a large
Thermoelectric Temperature proportion of the available energy
Control passes through the sample, giving Scan - Aurius only
Thermoelectric temperature control accurate, low noise measurements Wavelength scans with peak labelling
is provided by an internal controller. with excellent linearity. and peak tables. Time course plots
The controller is factory fitted and Kinetics
must therefore be specified at the Kinetic measurements with plots,
time of purchase. reprocessing of data
Kinetics M
Temperature control is provided for Multi-cell Kinetics linked with autocell
a single cell, sipette cell or a four changing, and plotting of reaction
cell changer. Provision is also curves
made for work below ambient
temperature. Temperature program- Validation
ming is also provided for thermal Absorbance and Wavelength validation
melt experiments. using certified standards or liquids.
Validation of optical bandwidth, etc.
Automatic Cell Changers Water Thermostatting Sampling Accessories

Four, six and eight cell changers are Water thermostatted holders are A complete range of sampling
available. A long pathlength holder available for single cells, sipette accessories is available, some of
is available for four cells. Water and cells, long pathlength cells and the these are shown and include cell
Thermoelectric temperature controlled four cell changer. A holder with pipe changers, 50µL and 5µL cell holders,
holders may also be used, as shown. port and special lid fitted is shown . film holder and microcell changer.
10
ORDERING
Spectrophotometers are supplied complete with cell AURIUS
holder, power cable, instruction manual and short
form operating instructions. VISIBLE RANGE
SUPER AURIUS 325-1100nm Wavelength range; 4nm Bandpass
325-1100nm Wavelength range; 4nm Bandpass.
1.8nm Bandpass, 190-1100nm wavelength range,
With integral printer
scanning.
1.8nm Bandpass, 190-1100nm wavelength range, UV/VISIBLE RANGE
scanning. With integral printer. CE 2021 Spectrophotometer
190-1100nm Wavelength range, 4nm Bandpass
Spectrophotometers CE 2041 Spectrophotometer
Special versions suitable for tablet dissolution 190-1100nm Wavelength range, 4nm Bandpass.
applications, using TD software. With integral printer
System 1 : Cells and Spares Spectrophotometers
Spectrophotometer with two silica cells, spare lamps, Special version suitable for tablet dissolution
spare fuses and dust cover. applications, using TD software
Example: CE2021 system 1 is a CE 2021 with spare System 1
deuterium and tungsten lamps, spare fuses, 2 silica Spectrophotometer, spare lamp(s) spare fuses,
cells and dust cover. 2 cells and dust cover.
Cell Holders Part No. Temperature Control - Water Part No.

Adjustable holder for sipette or single 10mm cell 1010 32 00 Holder for single or sipette cell 1010 33 00
Universal cell holder, 10mm cells upwards 1010 38 00 Holder for 4 cells - for autochanger 2020 36 15
Long pathlength cylindrical cell holder 1010 39 00 Lid and pipe port for water thermostatting 1010 05 00
Holder for solid samples or films 2020 33 00 Temperature Control - Thermoelectric
Ultra-microcell holder : 50µL cells 2020 39 00 Thermoelectric programme controller 7400 30 30
Ultra-microcell holder : 5 and 10µL cells 2021 30 00 Holder for single or sipette cell 2020 31 20
Cell Changers and Holders Holder for 4 cells with autochanger 2021 32 20
4 cell automatic changer (no holder) 2021 34 00 Temperature Programming
4 x 10mm cell holder for auto changer 2010 24 00 Thermoelectric programme controller 7400 30 30
4 x 50mm cell holder for auto changer 2010 36 00 Single 10mm cell holder 2020 31 20
6 cell automatic changer and holder 2021 36 00 Sample Stirring
8 cell tablet dissolution autochanger system 3021 37 00 Electronic controller for sample stirring 7200 31 01
4 microcell manual changer and holder 2020 38 00 Cell holder for sample stirring 2010 32 00
3 x Flowcell changer for water analysis 2020 35 00 Water thermostatted holder for stirring 2010 33 00
Sipette and Batch Sampling 4 cell holder for sample stirring 2010 34 00
Holder for 10mm sipette cell 1010 32 00 Lamps
Holder for sipette cells up to 50mm 2020 37 00 Deuterium lamp with hours indicator 2202 01 42
Batch sampler for 100 samples with pump 2021 82 02 Tungsten halide lamp - in pairs 2303 01 40
Return micro-sipette system no cell or holder 2021 21 00 Printers and Cables
Calibration Filters RS232c cable for 25 pin PC 2021 26 00
Holmium filter in holder 202 01 44 RS232c cable for 9 pin PC 2021 83 00
Didymium filter in holder 303 01 30 Parallel printer connection cable 8000 71 00
Set of 2 certified wavelength filters in holders 303 40 00 Dot matrix printer 8000 70 01
Set of 4 certified Absorbance filters in holders 594 44 00 Colour inkjet printer 8000 72 01
Set of 6 certified Absorbance filters in holders 594 66 00 Print rolls and ribbon for integral printer 2020 03 20
11
SPECIFICATIONS HIGHLIGHTS
Monochromator Littrow, 1200 lines/mm holographic grating
Optical Bandwidth 4nm - Aurius; 1.8nm Super Aurius BENEFITS
Wavelength Accuracy Better than ±1nm High Performance Scans
Straylight Typically less than 0.01% at 220 and 340nm
Derivative Spectra
Display Screen - LCD High resolution backlit with contrast control
Display Scrolling Provides 6 screen widths for viewing plots etc. Time Plots
Photometric Ranges Digital display of -0.3-3A, 0-200%T, 0-9999C Assay Formula Program
Photometric Accuracy ±0.005A or 1% whichever is greater
Photometric Noise Less than ±0.00004A (500nm RMS)
Stability Better than 0.001A/hour at constant temp. DataStream - Fast Data to PC
Multi-Wavelength Analysis 2 and 3 wavelength methods
Assay Program Enter multi-wavelength formula for automatic Display Scrolling - 6 Screens Wide
computation and formatting of any assay
Curve Fitting and Editing Linear, quadratic or cubic; up to 30 standards
Cell and Wavelength Programming Up to 8 cells and 10 wavelengths combined E-SEF Software Call-up
Method Storage Up to 30 methods stored in protected memory
Absorbance Ratioing 260/280nm or any two selected wavelengths Integral Printer Versions
Peak Seek Automatic search, baseline corrected
Reaction Kinetics Tabulated results, reaction plots, reprocessing
Method Storage
Batch Sampling 100 samples, internal control, screen display
Computer/printer interfaces Bi-directional serial RS232 and parallel ports Cell and Wavelength Programs
Sample Temperature Displayed and set from keyboard
Size 480 x 340 x 205mm Kinetics, Single/Multi-cell
Weight 16.8kg Visible; 18.6kg UV; + 0.9kg with printer
Power Requirements 110-250V, 50/60Hz; 120W/170W UV versions
Multi-Wavelength Assays
UV Lamp One Year Guarantee

SUPER AURIUS ADDITIONAL SPECIFICATION
Optical Bandwidth 1.8nm
Wavelength Scanning and Range 1-4000nm/min; 190-1100nm Compact - Only 48cm Wide
Photometric Plotting Ranges Keyboard entry of limits; or automatic
optimum PERFORMANCE
Baseline Flatness Better than ±0.002A (300nm/min) most of
range
Overlayed Spectra Up to 6 Spectra and derivatives with or Optical Bandwidth 1.8nm
without offsets
Spectral Reprocessing Spectra manipulated and replotted over any Straylight 0.01%
range
Dynamic Scan Recall Instant, automatic recall of last 8 scans
Spectral Storage Up to 100 spectra may be stored security Wavelength Precision 0.1nm
code protected
Derivative Spectra 1st and 2nd derivatives
Multi-cell Kinetics Up to 4 or 6 cells, with with plots and Stability ±0.001 A/hour
reprocessing
Band Quantification Automatic band area and height correction Accurate to 3A
Cecil Instruments policy is one of continuous development. We therefore reserve the right to change specification without notice. Full Validation Procedures

CAMBRIDGE CB24 6AZ ENGLAND ISO 9000
E-MAIL: info@cecilinstruments.com WEBSITE: www.cecilinstruments.com
January 2016
REFLECTANCE
SPECTROPHOTOMETERY
INTEGRATING
INTEGRATING SPHERE
Diffuse Reflectance Measurements Universal Reflectance Measurements
The integrating sphere allows a wide range of measurements. The ReflectaScan™ Spectrophotometer is
designed as a universal diffuse and
■ Diffuse and specular samples specular reflectance measuring instrument
■ Total hemispherical reflectance capable of scanning samples over the
wavelength range 190-1100nm. An integral
■
■
Specular excluded diffuse reflectance
Transmittance of turbid or scattering samples
SPECULAR REFLECTANCE printer plots results and an external printer
may be used.
■ Diffuse reflectance of powder samples
Integrating Sphere
Measurements of total hemispherical reflectance are made with or
without the spectral component eliminated. Spectral reflectance A dedicated, compact high performance
and sample transmission may also be measured. Spectralon™ integrating sphere is used for
diffuse reflectance and diffuse transmittance
measurements. The sphere unit has its
own silicon diode detector which uses a
separate signal channel within the
Spectrophotometer.
Colour Computations Using Diffuse

Reflectance
Onboard software provides automatic
analysis and computation of colour
samples when scanned.
Overlaid scans of two blue samples from
the Dulux paint range are shown; each is
specified by its British Standard number
shown on the chart.
The automatic analysis of the scans of two
very similar pink colours, also from the
Dulux range, is shown on the lower graph
which displays the overlaid scans. The
Peach Medley V Peach Swirl automatic analysis of each scan is shown
and the automatically generated table of
colour difference is also shown.
The specular component was excluded
from all scans.
Turbid or Scattering Samples

The sphere unit is equipped with a 10mm
pathlength cuvette holder so that
transmittance measurements may be
made for both turbid or normal samples.
Scattering solid films may also be
accommodated for measurement.
SCAN 105 SCAN 106 COLOUR DIFFERENCE

Interval: 5 nm Interval: 5 nm CIE dE*uv: 4.307
Illuminant: C Illuminant: C CIE dH*uv: 4.047
Observer: 2 degree Observer: 2 degree CIE dE*ab: 3.453
Tristimulus: X Y Z Tristimulus: X Y Z CIE dH*ab: 3.221
57.335 50.84 38.156 56.35 50.615 35.574
Chromaticity: x y z Chromaticity: x y z
0.392 0.347 0.261 0.395 0.355 0.25
CIE: L* a* b* CIE: L* a* b*
76.582 19.022 22.437 76.445 17.203 25.369
CIE: L* u* v* CIE: L* u* v*
76.582 44.353 28.665 76.445 43.23 32.821
Saturat. uv: 0.69 Saturat. uv: 0.71
Chroma uv: 52.81 Chroma uv: 54.277
HueAngle uv: 0.574 HueAngle uv: 0.649
Chroma ab: 29.415 Chroma ab: 30.652
HueAngle ab: 0.703 HueAngle ab: 0.596
SPECULAR REFLECTANCE
REFLECTANCE
Coated Lens Reflectance Specular Reflectance
The optical performance of the anti-reflection coatings on A dedicated specular reflectance unit is
opthalmic lenses is specifically catered for by the specular used for specular reflectance measurements
reflectance unit. A conical rest is provided to accommodate lenses of surfaces and coated opthalmic lenses.
up to 90mm diameter. Measurements are made with reference to The unit is ideal for quality assurance in
an uncoated lens and the system is excellent for the production the production of anti-reflection coatings
control of vacuum coated lenses. and the measurement of thin film
thickness, for instance in the semi-
conductor industry. Many of these systems
are now in use around the world.
Opthalmic Lens Transmission

The transmission characteristics of tinted
opthalmic lenses may readily be measured
using the CE 3077 lens mounting system.
The lens is accurately centred on a carrier
plate which is kinematically mounted on
the body of the lens holder.
Automatic Thin Film Measurement

The measurement of thin deposited films
is important in many optical and electronic
industries.
The CE3075 Specular Reflectance unit is

valuable for measuring the thickness of
thin transmitting films such as epitaxial
films of silicon oxide on a silicone wafer.
A reflectance scan against wavelength

produces a series of reflectance maxima
and minima, due to optical interference,
from which the ReflectaScan instrument
automatically calculates the film thickness
for films down to 0.1 microns thick.
Petrochemical Applications
The scan shown here gave a film thickness
Calculations using the transmission characteristics of
petrochemical samples is widely used in the industry. of 1.05 microns.
The overlaid transmission spectra of two petrochemical samples
are shown here as recorded by the CE3055.
Scan 100 is of a highly refined, water white, oil used as a reference Automatic Colour Computations
and scan 101 is of a pale yellow mineral oil.
On board software provides a wide range
The instrument automatically calculates the Saybolt and ASTM
colour reference as printed out below. of colour calculations including tristimulus
values, chromaticity, CIE L* a* b*, CIE L*
Oil Colour u* v*, whiteness etc.
Standard illuminant tables include CIE, a, b

and c and CIE D50, D65 and D75. Observer
tables include 2º and 10º angles.
SCAN 100 SCAN 101

Interval: 5 nm Interval: 5 nm
Illuminant: C Illuminant: C
Observer: 2 degree Observer: 2 degree
ASTM Colour: 0.2 ASTM Colour: 0.8
Saybolt: 28.3 Saybolt: -16
ORDERING
ORDERING
CE 3055 ReflectaScan™ Reflectance
Spectrophotometer
SPECIFICATION
SPECIFICATIONS 4nm bandpass, 190-1100nm
wavelength range, built in integrating
Optical Monochromator Littrow using 1200 L/mm holographic grating
Optical Bandwidth 4nm SPECIFICATIONS
sphere signal channel, cell holder,
power cable and operators manual.
Wavelength Range and Accuracy 190-1100nm, better than ±1nm
Wavelength Reproducibility ±0.1nm
CE 3073 Integrating Sphere Unit
Spectralon™ sphere with integral
silicon detector, 10mm turbid sample
cuvette holder, film holder, 0˚ and 11˚
Straylight Typically <0.01% at 220nm and 340nm
sample rests and internal rotatable
Display Screen - Backlit LCD Displays menus, plots etc with six screen mirror system.
widths of viewing available by scrolling
Photometric Ranges Display of -0.3-3A, 0-200%T, 0-9999C, %R CE 3075 Specular Reflectance Unit
Photometric Accuracy ±0.005A or 1% whichever is greater With flat sample rest, conical lens
Photometric Noise Less than ±0.0002A (500nm) mounting rest, mounted front surface
Baseline Flatness Better than ±0.002A most of range aluminised plain reference mirror and
Baseline Stability Better than 0.001A/hour, 500nm mounting thumb screws.
Overlayed Scans Scans and derivatives with or without offset
Spectral Reprocessing Scans manipulated; replotted over any range CE 3077 Opthalmic Lens Holder
Integral Printer Prints scans and data Holder for transmission measurements
Scan Storage Up to 100 stored security code protected with lens centring and mounting plate,
Curve Fitting and Editing Linear, quadratic or cubic; up to 30 standards and mounting thumb screws.
Wavelength Programming Up to 10 wavelengths
Method Storage Up to 100 methods stored in safe memory 8000 70 01 Dot matrix printer
Derivative Spectra 1st and 2nd derivatives Includes cable.
Real Time Clock Timed and dated reports 8000 72 01 Colour Ink Jet printer
Computer/printer interfaces Bi-directional serial RS232C and parallel port Includes cable.
Size & Weight 480 x 340 x 205mm, 19.5kg
Power Requirements 110-250V, 50/60Hz, 170W 8000 73 01 Laser printer
Integrating Sphere Unit OPTIONAL SOFTWARE

2” diameter Spectralon™ sphere with integral silicon diode detector, lever
operated internal beam reflecting mirror, 0˚ sample rest for specular excluded DataStream
measurements, 11˚ sample rest for total hemispherical reflectance measurements, Fast transfer of data to a PC for use
10mm cuvette holder for turbid and scattering samples, and film holder. Mounted with Excel or other spread sheets.
Spectralon standard.
Colour Scan Software
Specular and Lens Reflectance Unit For calculation of tristimulus
Horizontal sample stage with rest for flat samples, conical rest for lenses, integral chromaticity, CIE L*a*b*, CIE L*u*v*
beam polarizer and mounted front aluminised mirror reference standard. and thin film thickness etc.
Opthalmic Lens Holder
Quant S
Holder for transmission measurements. Kinematically mounted lens centring plate
Quantitation of corrected bands,
with lens clamping facilities.
overlayed spectra, spectral storage.
ORDERING ACCESSORIES AND SPARES

SPARES Program W
Lamps Wavelength program for up to 10
Deuterium lamp with hours indicator 2202 01 42
measurements at a fixed wavelength.
Tungsten halide lamp - in pairs 2303 01 40
Printers, Cables
Validation
RS232 9 pin PC cable and protocol manual 2021 83 00
Absorbance validation using certified
Dot matrix printer, includes cable 8000 70 01
standards, wavelength validation
Colour ink jet printer, includes cable 8000 72 01
using certified standards, optical
Printer connection cable 8000 71 00
bandwidth, etc.

CAMBRIDGE CB24 6AZ ENGLAND ISO 9001 : 2000
E-MAIL: info@cecilinstruments.com Email: www.cecilinstruments.com
FOOD ANALYSIS
BEVERAGE ANALYSIS
™
™
PROGRAMMED FOOD AND
DietQuest ™ for Food and Beverage Analysis
Cecil Instruments offer three DietQuest™ spectrophotometers
fully pre-programmed for both food and beverage analysis.
Methods include single and multi-wavelength measurements
and spectral scanning. The DietQuest™ UV and the VIS
versions are as shown on the right.
All the pre-programmed methods are defined by national and
international bodies and trade associations including the
following:
AACC, AOAC, ALVA, APA, ASBC, BS-EN, CAA, EBC, DIN, FDA,
FIL-IDF, GUdRI, ICC, IFU, IoB, IOCCC, ISO, MAFF, MEBAFA,
MEBAK, NEN, NF, OIV, OJEC.
Simple Rapid Operation

Even the most complex assay is carried out simply by
selecting from the displayed menu the required method, Concentration Curve Fit
inserting blanks, references and samples as prompted. Just Where concentration is not linearly related to Absorbance, a
press the run key for automatic measurement and calculation quadratic or cubic curve may be fitted to up to 30 standards
of the result. All results are displayed on screen and printed, or replicates and edited before use. A straight line may be
timed and dated, on the integral or external printer. fitted to linear results and 30 curves may be stored security
protected.
User Programming
Never before has user programming of assays for automatic
Sipette Sampling
measurement and computation been so simple and rapid. Samples may be handled rapidly and without cell handling
using the sipette sampling system. Sample volume is user
Cecil Assay Programme software allows any assay to be selected and low cross contamination occurs even with
formulated for up to 10 wavelengths. Rapid entry of text and samples as small as 300µL.
formulae and setting a wavelength program is all that is
required.
Up to 20 formulae, each with up to 76 characters, may be

combined for the most complex method and cell
programming may be incorporated for multi-sample
measurements.
Flexibility of Application
DietQuest™ spectrophotometers additionally have all the
functions of a high quality spectrophotometer and may
therefore find wide application in a testing laboratory. Where
only visible determinations are required the ‘DietQuest : VIS’
is offered. The high performance double beam instrument
‘DietQuest : D/B’ completes the range and is shown below.
Reaction Kinetics
Enzyme reaction methods using kits supplied by Boehringer
- Mannheim, Roche, Diogen, Megazyme etc. have been pre-
programmed for a single cell or for 4 or 6 cells in an
automatic changer.
Reaction curves are displayed on screen and regression
analysis applied to the user selected part of the curve. A
number of the assays are simple end point measurements.
Sample temperature control, where required, is available
using either water circulation or Peltier Thermoelectric cell
holders and controller.
DataStream - Data to a PC
Both raw, and processed and formatted data may be rapidly
exported to a PC for incorporation into a spreadsheet or
mathematical package of the users choice, e.g. Excel, Grafit
etc. for manipulation and report generation.
2
D BEVERAGE ANALYSIS
Pre-programmed Convenience
The versatility of the DietQuest™ spectrophotometers is illustrated by the partial list of pre-programmed tests
shown. Tests may be modified, or new tests programmed and stored by the user.
Food Analyte Method Beverage Analyte Method

Baby food Nitrate MEBAFA Beer Copper EBC
Barley β-Glucan EBC Beer Carbohydrate EBC
Barley α-Amylase ICC Beer Nitrate MEBAK
Caramel Colour EBC Beer Bitterness AOAC
Cereal adjuncts Colour EBC Beer Colour AOAC
Cheese Citric acid AOAC Beer D-Glucose MEBAK
Egg Carotenoids AOAC Beer Bitterness EBC
Egg, yolk Colour AOAC Beer Colour EBC
Flour Pigment AOAC Beer Polyphenols EBC
Foodstuffs Sulphite UDC Beer Vicinal Diketones EBC
Macaroni Carotenoids AOAC Beer, low alc. Ethanol EBC
Malt Colour EBC Beverages, coffee Caffeine AOAC
Malt β-Glucan EBC Beverages, non alc . Caffeine AOAC
Malts Colour EBC Beverages, tea Caffeine AOAC
Milk, dried Lactic acid IDF Diabetic beer D-Glucose MEBAFA
Olive oil Quality OJEC Fruit juice Glycerol MEBAFA
Preserves Glucose, Fructose MEBAFA Fruit & veg. juice L-Lactic acid BS-EN
Seafood Histamine AOAC Malt beer Colour EBC
Spice, pepper Piperine AOAC Milk L&D-Lactic acid DIN
Sugar Colour ICUMSA Orange juice Adulteration AOAC
Tomato ketchup Acetic acid MEBAFA Spirits, distilled Fusel oil AOAC
Wheat α-Amylase ICC Wine Colour OIV
Application : Adulteration of processed Florida Orange Juice

Juice must not contain pulpwash solids. Using method AOAC 986.14 adulteration is measured by comparison of
spectra, and using the adjusted sum of Absorbances at 443, 325 and 250nm and their ratio at 443 : 325nm.
SOFTWARE EXTENSION MODULES

Program C Kinetics M
Cell program for 4, 6 or 8 cells Multicell Kinetics
Program W Quant S
Wavelength program for up to 10 wavelengths Spectral storage, overlayed spectra, quantification of
corrected bands etc.
Scan - DietQuest : VIS only DataStream
Wavelength scan with peak labelling and time-course plots Fast data transfer to PC; PC control of instrument
Kinetics Validation
Kinetic measurements with plots and reprocessing Validation of Absorbance, wavelength, bandwidth, straylight etc .
3
™
SPECIFICATIONS
CE 3410 CE 2410 CE 7210
DietQuest : UV DietQuest : VIS DietQuest : D/B
Optical System Single beam Single beam Double beam

Grating Monochromator Littrow, 1200 L/mm Littrow, 1200 L/mm Czerny Turner 1200 L/mm
Wavelength Range 190-1100nm 325 - 1100nm 190-900nm
Wavelength Accuracy Better than ±1nm Better than ±1nm Better than ±0.5nm
Optical Bandwidth 1.8nm 4nm 1.8nm
Wavelength Precision Better than 0.1nm

Photometric Noise Better than 0.00004A at 1A, 500nm, RMS
Photometric Range -0.3-3A
Scan Speed 1-4000nm/min 1-4000nm/min

Baseline Flatness Better than ±0.002A p.t.p. Optional scan available Better than ±0.001A p.t.p.
Photometric Accuracy Better than ±0.005 at 1A Better than ±0.004A at 1A
Method Storage 30 user defined methods security code protected

Curve Fit Linear, quadratic and cubic curves, to 30 concentration standards or replicates
Computer/Printer Interface Bi-directional serial RS232 and parallel ports
Stability Better than 0.001A/hr Better than 0.001A/hr Better than 0.0001A/hr
Size and Weight 480 x 340 x 205; 19.5kg 480 x 340 x 205; 17.7kg 635 x 410 x 210; 33kg
ORDERING INSTRUMENTS
Instruments are supplied complete with cell holder(s), power cable, manual and brief operating instructions.
DietQuest : UV, CE 3410 DietQuest : VIS, CE 2410 DietQuest : D/B, CE 7210

190-1100nm range, 1.8nm 325-1100nm range, 4nm 190-900nm range, 1.8nm
bandwidth, integral printer bandwidth, integral printer bandwidth, double beam
CE3410 / CE2410 CE7210 CE3410 / CE2410 CE7210
Cell Holders Temperature Control - Water
Micro sipette 10mm cell holder 1010 32 00 503 26 00 Single or sipette cell holder 1010 33 00 503 36 00
Adjustable holder for micro cell 6600 57 00 4 cell holder 2020 36 15 202 36 16
Rectangular cells up to 100mm 1010 38 00 595 27 00 2 x 4 cell holder, sample and ref. 202 36 15
Cylindrical cells up to 100mm 1010 39 00 595 26 00 6 cell holder 5500 36 15
Cell Changers Thermoelectric Control
4 cell auto changer, no holder 2021 34 00 9070 31 00 Thermoelectric Controller CE 2024 CE 2024
Holder for 4 x 10mm cells 2010 24 00 2010 24 00 Single or sipette cell holder 2031 31 20 8020 56 00
6 cell auto changer, no holder 9070 34 00 4 cell holder 2021 32 20 9070 57 00
6 cell auto changer and holder 2021 36 00 2 x 4 cell holder, sample & ref. 9070 58 00
Holder for 6 x 10mm cells 5500 33 00 6 cell holder 9070 59 00
4 position microcell changer 2020 38 00 505 38 00 Printers and Cables
Sipette and Batch Sampling RS232 cable for 25 pin PC 2021 26 00 2021 26 00
Sipette system, no holder or cell 2021 21 00 9070 21 00 RS232 cable for 9 pin PC 2021 83 00 2021 83 00
10mm sipette cell 202 07 02 202 07 02 Printer connection cable 8000 71 00 8000 71 00
40 batch sampler with pump 2021 82 00 2021 82 00 Dot matrix printer 8000 70 01 8000 70 01
100 batch sampler with pump 2021 82 02 2021 82 02 Colour ink jet printer 8000 72 01 8000 72 01
Lamps Print rolls and ribbon 2020 03 20
Deuterium lamp with indicator 2202 01 42 2202 01 42 Certified Calibration Filter Sets
Tungsten halide lamp - in pairs 2303 01 40 2303 01 40 2 Wavelength filters 303 40 00 303 40 00
Sample Stirring 4 Absorbance filters 594 44 00 594 44 00
Electronic stirrer 7200 31 01 7200 31 01 6 Absorbance filters 594 66 00 594 66 00
Cell holder 10mm cell 2010 32 00 579 02 00 2 Wavelength and 6 Abs. Filters 594 77 00 594 77 00
ISO 9001:2008
TELEPHONE: +44 (0) 1223 420821 FAX: +44 (0) 1223 420475 CERTIFIED COMPANY
4
UV:SPECTROPHOTOMETERS
TRUE SYMMETRICAL DOUBLE BEAM
POWERFUL INTEGRAL SOFTWARE
FULL REFERENCE BEAM SPACE
DISPLAY SCROLLING OF SCANS
CHOICE OF OPTICAL BANDWIDTH
SUPERB VERSATILITY
ACCURACY : FLEXIBILITY : FAST-OPERA
FULL VALIDATION PROCEDURES
WIDEST ACCESSORY RANGE

Variable or Fixed Bandwidth Powerful Integral Control
The Aquarius spectrophotometers offer very high Rapid operation with ease of use is provided by the
performance with fixed optical bandwidth of less powerful software built into the computing system of
than 2nm pharmacopoeia compliant, 1nm, or the Aquarius instruments. The standard software is
variable optical bandwidth of 0.5, 1, 2 and 4nm for remarkably comprehensive but may be expanded, at
high resolution applications. any time, by E-SEF to release additional software
modules when required.
The spectrophotometer's performance and powerful
software are only normally available with much Rapid Menu Operation
more expensive instruments.
The large backlit high resolution LCD screen displays
Double Beam Optical System menus for all applications, with clear prompts for all
entries and procedures. Scans, plots, curves and data
The fully symmetrical double beam system of the are all displayed on the screen and may be reprocessed
Aquarius instruments provides the highest accuracy or manipulated before storing or producing a printout.
of measurement coupled with very high stability
with time. Secure Integral Memory
Generous built in secure memory allows scans, curves,
Both sample and reference beams are provided with plots, kinetic curves, as well as methods to be stored
the same space facilities, thus catering for the tamper proof protected by personal security codes.
widest possible range of experimental requirements
and not limited to just a simple cell in the reference Any scans, plots or data may be recalled from memory,
beam. with assured integrity, for reprocessing, manipulation
and printing as required.
Scans Viewed By Scrolling
By scrolling information across the display screen,
Dynamic Scan Recall
the effective width of the screen is a massive The Aquarius instruments are provided with the ability
430mm. to store the last 25 or more scans or plots without the
need to carry out a scan storage procedure. The scans
Long scans, plots and other data may be viewed in or plots are automatically stored as they are made and
much more detail, than with other instruments. may be recalled from a list on the display.
2
TRUE SYMMETRICAL DOUBLE BEAM

SOFTWARE

Software Enhancement By E-SEF Benefits
This unique design feature - Encoded Software Enhancement Facility Variable or Fixed Bandwidth
- provided by Cecil Instruments, enables any required additional
software module to be released by an encoded entry. The encoded
entry required is obtained by telephone, fax or E-mail, enabling you
Large Backlit LCD Screen
to expand your library of methods, without delay, when a new Screen Scrolling
application arises within your laboratory.
CHOICE OF OPTICAL BANDWIDTH Scrolled Screen Width
Method Operation 430mm
SUPERB VERSATILITY
Any procedure may be stored as a method in the security code
protected integral memory of the Aquarius instruments. When a
Dynamic Scan Recall
method is recalled, all instrument parameters are automatically set, Full Reference Sample Space
without operator intervention, thus ensuring that the original
ACCURACY : FLEXIBILITY : FAST-OPERATION
conditions are exactly replicated. Method Storage
Scan Storage Spectral Storage

Scans, plots and curves may all be stored, for future use, with Curve Storage
confidence in the integrity of the data. Integral memory is provided
for up to 100 scans, protected by the operators personal security Password Protected Storage
codes.
Multi/Single Cell Kinetics
Reprocessing Operates in 6 Languages
Although scans may be in absorbance, transmittance or derivative
format, any scan may be transformed to an alternative format by the
very rapid reprocessing facility provided. Performance
Bandwidth < 2nm or 1nm
Fully Documented Scans
Variable 0.5, 1, 2, 4nm
As shown below all scans are fully documented on screen and in
hard copy. Stability ±0.0001A/H
Tables of all peaks and valleys are produced together with Scan rate 1 to 4000nm/min
instrument, sample and operator details. Scans recalled from the
instruments integral memory or by dynamic scan recall are also fully Wavelength Precision 0.1nm
documented.
Straylight <0.02%
The screens shown are not two separate displays but part of a
continuous display viewed by scrolling. Validation Software
3

SPACE
Sampling SCROLLING
DISPLAY Flexibility OF SCANS
The design of the double beam optical sampling allows
almost identical space and facilities in both the sample and
reference beams. Multi-cell reference sampling, with
temperature control is therefore possible.
Operation is not limited to a single standard cell in the

SUPERB VERSATILITY
reference beam as in some other instruments.
Reflectance and Gel Scanning

Accessories include fixed angle and variable angle
ACCURACY : FLEXIBILITY
reflectance units and a gel scanning system for:gels
FAST-O
up to
100mm long.
DataStream
Data may be transferred from the spectrophotometer to a
PC, or the software used to control the instrument from a
Multi-User Convenience WIDEST ACCESSORY RANGE
PC. Datastream software, developed by Cecil Instruments,
has a wide range of facilities including graphical and
Aquarius double beam instruments were designed
tabular presentation, normalisation of overlayed traces,
for the greatest convenience in a laboratory where
point and peak pick using a cursor, library facilities, data
multi user operation is required.
storage in files on disk, history logging and copy and paste
All methods, stored scans, plots, curves and data to other applications such as Excel, Word etc.
remain in secure integral memory, tamper
protected by passwords, readily accessible to all
operators without the possibility of mislaid disks The photograph below shows an Aquarius instrument in
or plug in card modules. use in Cecil Instruments applications laboratory.
4
DISPLAY
CHOICE
Curve and LineOF OPTICAL BANDWIDTH
Fitting
A straight line, quadratic or cubic curve may automatically be fitted to a
set of up to 30 standards. Fits may be made with an intercept or forced
SUPERB VERSATILITY
through zero. Replicate standards may be used for any given
concentration. All entries are prompted for simplicity of operation. A
quadratic curve fit is shown here.

Editing Suspect Standards and Curve Storage STANDARD SOFTWARE
Suspect standards Wavelength Scan
may be observed
by inspection of the
screen display and
WIDEST ACCESSORY RANGE either deleted or
Peak and Valley Tabulation
substituted by a
replicate standard. Derivatives 1st-4th
Up to 30 curves
may be stored in Time/Rate Plot
integral memory,
security code
protected for future Dynamic Scan Recall
use.
Auto Sequential Scans
Derivative Spectra Scan Storage
Derivative spectroscopy is a powerful technique for revealing hidden

information or enhancing fine detail in a spectral scan. Bands masked Scan Overlay
by other overlapping bands or distorted by a sloping background may
be accurately located for quantification.
Scan Reprocessing
Derivative plots may be displayed on their own or superimposed on a
wavelength scan, with or without an offset. The degree of smoothing
used is operator entered and ranging is normally selected to be Method Storage
automatic.
Curve/Line Fit
Curve Storage
Editing of Standards
Absorbance Ratio
Absorbance Difference
Measurements in A, C, %T
5
SUPERB VERSATILITY
Optical Bandwidth
The Aquarius range provides the choice of fixed
bandwidth of less than 2nm, 1nm, or variable
ACCURACY : FLEXIBILITY : FAST-OPE
bandwidth of 0.5, 1, 2 and 4nm. The variable
resolution performance of the CE 7500 is ideal for
samples with sharper features requiring high
resolution - particularly vapours and some liquid
samples.
The performance of the Aquarius CE 7500 is

illustrated in the plots shown here of Benzene
Vapour at two different bandwidths, 0.5nm and
2nm overlayed.
Also shown here is the spectrum of iodine vapour

near 560nm.
The broad flat bands resulting from a scan at 2nm

bandwidth are well resolved when scanned at
0.5nm bandwidth.
A 1 nm optical bandwith is provided by the CE 7400S

version of the Aquarius, for applications requiring
this particular performance.
Micro-Sipette Sampling
Samples of 300µl or less, and multiple samples, may be
measured with very low cross contamination.
Samples are measured in the same cell enhancing

accuracy and speed of measurement. The pump, in the
sample compartment, is controlled by the instrument's
microprocessor.
Ultra-Micro Sampling
Valuable samples, such as biological samples prepared
with costly reagents, may be measured using a special
ultra-microcell and holder. With the system, samples of
50µl or even less may be accurately measured.
Tablet Dissolution
The CE 7460 and CE 7560 versions of Aquarius are fitted
with a special cell compartment and pipe port for tablet
dissolution assays.
The instrument may be used with either a six or eight

cell automatic changer.
6
SUPERB VERSATILITY
VERSATILITY

Reaction Kinetics
Both single cell and multi cell Kinetics are available with reaction plots to screen and printer. Regression analysis
is applied to the user-selected portion of reaction curves and results are reported in Units/Litre. Data may be
reprocessed as required and curves stored. Sample temperature control is available for single cells and four and
six cell changers.

Advanced Kinetics
An example of advanced kinetics is shown below where time based reaction data generated by the
spectrophotometer has been transferred into Grafit, fitted to a curve and transformed into a more suitable format
for mathematical interpolation and calculation. In this particular example, the basic data plot can yield the limiting
rate (Vmax), the Michaelis constant (Km) and the initial linear portion (Vmax/Km).
This has been transformed into the Eadie-Hofstee format and plotted to give better precision in the calculation of
these important factors and constants.
Other transformations are available such as Lineweaver-Burk and the Hill plot for allosteric enzyme systems.
PC Data Manipulation
Data may be transferred to a PC for use with spread

sheets and other mathematical software packages.
More advanced reaction kinetics data manipulation

may be readily performed with Grafit software. Linear,
non-linear or polynomial curve fitting is easily
accomplished together with non-linear regression
analysis. A wide selection of standard equations are
provided, together with an equation editor for
customised equation fitting.
Data may also be transformed into other mathematical

formats for plotting and calculation.
Band Quantification
Accurate quantitative assays using band area or height,
corrected for background absorption, are exceptionally
easy to carry out with the easy to use Aquarius
instruments.
Analytical results are displayed and printed with

construction lines enabling the quality of the
parameters to be assessed.
The procedure may be stored as a method with security

code protection in the integral memory of the
instrument.
7
SUPERB VERSATILITY
ACCURACY : FLEXIBILITY
FULL VALIDATION PROCED

WIDEST ACCESSORY RAN

Double Beam Wavelength Scans Powerful Quantification
Scans at up to 4000nm/min are presented on screen or Many quantitative methods are available including band
printed with all peaks annotated with wavelength and area computation with background correction, differencing
absorbance. Tables are also presented listing all peaks and and stripping of spectra, wine and colour assays,
troughs with values. mathematical calculations between several wavelengths,
protein and DNA assays.
Display Scrolling Curve and Line Fitting

Use of the display scrolling facility enhances the effective A straight line, quadratic or cubic concentration curve may
screen size and enables spectra and data up to 430mm wide be fitted to up to 30 standards, with or without an intercept at
to be viewed. the origin. Standards may be deleted and replaced and a
new curve fitted.

The last 25 or more scans or plots are automatically retained Single cell kinetic measurements are possible with plots and
for recall by entering their scan sequence number and may data displayed on screen. Data may be reprocessed,
be reprocessed, overlaid or transferred to protected memory regression analysis applied and curves stored, security code
for future recall. protected against tamper or deletion.
Overlaid Spectral Scans Multi-Cell Reaction Kinetics

Up to eight scans, including stored scans, may be overlaid, Up to four or six cells are automatically measured with all
with or without offsets for convenient comparisons or reaction curves displayed on screen. Each curve may be
further manipulation. selected for processing with the results of regression
analysis printed.

New wavelength scans or scans from store may be Assays are provided using either two or three wavelengths.
presented on screen and the difference spectra produced. A wine colour assay is provided which may use up to ten
The scans may be normalised enabling spectral stripping to different wavelengths.
reveal impurities or additives.
Derivative Spectra DNA and Protein Assays

Derivative spectra, up to fourth are readily produced, with DNA and Protein assays may be made using either the 2 or 3
selectable smoothing, either alone or superimposed on the wavelength methods of Warburg and Christian. Also
fundamental, with or without an offset. Scaling is automatic programmed are techniques and analytical procedures for
with all peaks and troughs quantified. assays by Lowry, Bradford, Biuret and BCA methods.
Scan Storage Band Area and Height Computation

Up to 100 spectra may be stored in safe memory for future Band areas with correction for sloping background
recall. Each spectrum is automatically allocated a recall absorption are readily quantified, with all details and
number, when stored, and may be security code protected construction displayed. Band areas may also be computed
against tamper or deletion. for derivative spectra to facilitate difficult quantification.

Automatic scans, single or repeat may be made of a cell Using the various cell changers, measurements may be
program of up to 4, 6 or 8 cells. A programmed sequence of made on up to 4, 6 or 8 cells at a single wavelength with
scans may also be made of a single sample. cycle time and number of cycles entered by the operator. A
cell and wavelength program may be combined.

Changing samples or flows may be examined and displayed Measurements may be programmed for 10 different
by making a time course plot. Data may be reprocessed and operator selected wavelengths with the cycle time and
stored, security code protected, with an automatically number of cycles selected by the operator. A wavelength
assigned recall number. and cell program may be combined.
8
:F
FAST
AST-OPERA
-OPERATION
TION
EDURES
NGE
Integral Control System Variable or Fixed Bandwidth
All control software is within the instrument, tamper proof, Optical bandwidth selectable down to 0.5nm is provided by
providing easy menu driven operation with massive safe the CE7500 and allows the fine structure of narrow bands to
storage for scans, plots, curves etc. PC control and data be resolved which remain unresolved at just under 2nm
transfer to a PC are provided. bandwidth in instruments such as the CE7400.
Equal Sample and Reference Facilities Double Beam Optical System

Identical and generous sample and reference sampling The fully symmetrical optical system measures sample and
facilities widens the scope of measurement, for example, to reference simultaneously for ultimate accuracy, precision
enable multi-cell sampling in both beams and accommodate and baseline flatness. Long term stability is up to ten times
bulky accessories in the reference beam. better than single beam systems.
Method Operation and Storage Automatic Cell Changers

Up to 30 sets of instrument parameters, including Automatic changers are available for 4, 6 or 8 cells,
concentration curves may be stored, security code protected. providing rapid sample changing or the facility for full
When recalled, instrument parameters are automatically set, programming.
avoiding errors.
Multi-Language Operation Sipette Sampling

The operator may select from the six operating languages Samples down to 300 microlitres may be measured in a
provided. Full Alpha Numeric entry is provided so that 10mm cell. Sampling is under control of the instrument’s
results may be fully documented for GLP. microprocessor system. Samples may be saved or pumped
to waste.
Formula Entry and Computation Ultra-Micro Sampling
Mathematical formulae may be entered enabling complex Samples of 50µl and less may be accurately measured
calculations involving many wavelengths and cells to be assisted by the very small beam size of only 7 x 1mm. All
carried out automatically. normal cells up to 100mm pathlength are accommodated.
Software Enhancement By E-SEF Sample Temperature Control

E-SEF - Encoded Software Enhancement Facility - allows Water and thermoelectric temperature control are available
software modules to be enabled using encoded numbers for single cells and automatic cell changers. Temperature is
provided by telephone, fax or E-mail. Software set from the control panel and displayed on the screen in the
requirements may be tailored, at any time, as required. latter case.
Data Storage and Protection Dissolution Testing

Data, including 100 spectra, concentration curves, kinetics Versions of the instruments fitted with a special cell
and time course plots may be stored in safe memory, compartment with piping port, are available for dissolution
security code protected against tamper or deletion using a testing using Cecil TD software and a PC. Six and eight cell
four digit password. changers are available.
Reprocessing Batch Sampling

Scans, time course plots, reaction curves and gel scans may Automatic batch sampling for up to 100 samples is available.
all be reprocessed to any required format on the display The pump fits within the sample compartment and volume
screen and then plotted. etc is set from the control panel and displayed on screen.
Batch number entry is provided.
Performance Validation Specular Reflectance

Software and certified standards enable wavelength Special accessories enable specular reflectance
accuracy, absorbance accuracy, bandwidth, stray light etc. to measurements to be made. Both fixed angle and variable
be validated. A timed and dated report includes the serial angle reflectance accessories are available. The latter is
number of the instrument. particularly useful for film thickness measurements.
9
ACCURACY : FLEXIBILITY : FAST-OP
FULL VALIDA
VALIDATION
TION PROCEDURES
Physical Sciences Applications

The near infrared region to 1100nm is covered by both Absorbance accuracy is most conveniently measured
instruments maintaining excellent performance. using certified filter sets calibrated by Cecil
Measurements beyond 900nm can be of value in Instruments against NPL certified standards.
physical science applications such as the Wavelength calibration is also carried out using
measurement of optical filters, heat absorbing filters
and semi-conductors. certified filters.
The transmission spectra of two different filters is

shown here overlayed, demonstrating their
characteristics beyond 900nm.
Straylight
Straylight is measured following the Pharmacopoeia
(EP/BP) test, using a 1.2% w/v solution of Potassium
Chloride in water measured against water in the
reference beam.
The more widely accepted ASTM tests at 220nm and
340nm are also available.
Instrument Validation
Assessment of instrument performance is readily
carried out using the validation software module built
into all Aquarius instruments and released by E-SEF,
‘Encoded software enhancement facility’.
Laboratories subject to regulatory control, or following

G.L.P. are provided with a range of Pharmacopoeia
(EP/BP/USP) tests.
As well as these tests, tests using filters traceable to

national standards with certificates of calibration
issued by Cecil Instruments are available.
Pharmacopoeia and other tests are shown here.
Optical Bandwidth Absorbance Accuracy
The optical bandwidth of the instrument may be The Pharmacopoeia (EP/BP) test for absorbance
validated by measuring the half height width of the accuracy, using a 60mg/L solution of Potassium
narrow deuterium emission line at 656.1nm. The Dichromate in a 10mm pathlength cuvette, is shown
measurement is carried out automatically. here.
10
WIDEST ACCESSORY
ACCESSORY RANGE

All Aquarius instruments are fully programmable. Cell programmes
may use four, six or eight cell changers, with a single autozero or
individual zeros for each cell.
Wavelength programmes are provided for up to ten different
wavelengths and cell and wavelength programmes may be
combined.
Sampling and Temperature Control
A complete range of sampling accessories is available. A selection

of auto-sample changers, some with water temperature control, is
shown on the left. Thermoelectric single, four, four plus four and
six cell changers are shown above. Control is through the
instrument microprocessor system with screen display of
temperature.
Cell Holders Temperature Control - Water Circulation
Single cell holders - sample or reference 505 26 00 Single or micro sipette cell holder 503 36 00
Micro sipette 10mm cell holder 503 26 00 4 cell holder 202 36 16
Adjustable holder for micro cell 6600 57 00 2 x 4 cell holder, sample or reference 202 36 15
50µl ultra microcell holder 8020 39 00 6 cell holder 5500 36 15
Holder for rectangular cells up to 100mm 595 27 00 Temperature Control - Thermoelectric
Holder for cylindrical cells up to 100mm 595 26 00 Thermoelectric Control System 7400 31 00
Cell Changers Holder for single or sipette cell 8020 56 00
Auto changer for 4 or 4 + 4 cells, requires holder 9070 31 00 4 cell holder 9070 57 00
Holder for 4 x 10mm cells sample or reference 594 54 00 2 x 4 cell holder 9070 58 00
Auto changer for 6 cells, requires holder 9070 34 00 6 cell holder 9070 59 00
Holder for 6 x 10mm cells 5500 33 00 Sample Stirring
Holder for 6 x 20mm cells 5500 34 00 Electronic stirrer and sample cell holder 7200 31 00
Automatic 8 cell changer with holder 9070 32 00 Electronic stirrer and sample and reference holder 7200 32 00
4 position microcell holder 505 38 00 Gel Scanning
Sipette and Batch Sampling Gel scanner - requires trough or film holder 9070 39 00
Return sipette system, requires holder and cell 9070 21 00 Gel trough - 5mm x 100mm silica 570 07 02
10mm sipette cell 202 07 02 Printers and Cables
20mm sipette cell 202 07 51 RS232 cable for 25 pin PC with protocol manual 2021 26 00
40mm sipette cell 202 07 54 RS232 cable for 9 pin PC with protocol manual 2021 83 00
Sipette 10mm cell holder 503 26 00 Laser Printer 8000 73 01
Batch sampler for 40 samples with pump 2020 82 00 Dot matrix printer 8000 70 01
Batch sampler for 100 samples with pump 2020 82 02 Colour ink jet printer 8000 72 01
Reflectance Calibration Filters
Fixed angle reflectance accessory - pair CE 575 Holmium filter in holder 202 01 44
Variable angle reflectance accessory CE 574 Didymium filter in holder 303 01 30
Lamps Set of 2 certified Wavelength filters in holders 303 40 00
Deuterium lamp with hours indicator 2202 01 42 Set of 4 certified Absorbance filters in holders 594 44 00
Tungsten halide lamp - in pairs 2303 01 40 Set of 6 certified Absorbance filters in holders 594 66 00
11
ORDERING
ORDERING
< 2nm pharma-compliant Optical
bandpass. Wavelength range 190-1100nm.
SPECIFICATIONS
SPECIFICATION
Optical System Fully symmetrical double beam system CE 7400S Spectrophotometer
1 nm Optical bandpass.
All mirrors coated for long life SPECIFICATIONS
Wavelength range 190-1100nm.
Monochromator Modified Czerny-Turner design using 1200
L/mm holographic diffraction grating 0.5, 1, 2 and 4nm Optical bandpass.
Detectors Two silicon diode detectors Wavelength range 190-1100nm.
Self Test and Calibration Automatic at switch on CE 7200 Spectrophotometer
Wavelength Range and Accuracy 190 - 1100nm; 0.5nm <2 nm Optical bandpass.
Wavelength range 190-900nm.
Wavelength Readout Digital display on screen CE 7460/CE 7560 Dissolution Spectros
Wavelength Selection By GOTO Key and keypad entry Suitable for tablet dissolution monitoring
using a choice of dissolution software and
Optical Bandwidth < 2nm CE7400; 0.5, 1, 2, 4nm CE7500 an automatic six or eight cell changer.
Stray Light Less than 0.02% at 220nm, NaI; and 0.02%
8000 70 01 Dot Matrix Printer and Cable
at 340nm, NaNO2
Display Screen Large backlit LCD displays spectra, menus, 8000 72 01 Colour Ink Jet Printer and Cable
data, prompts and instrument status. 8000 73 01 Laser Printer and Cable
Display Scrolling Provides viewing width of 430mm
Derivative Spectra 1st to 4th derivatives SOFTWARE MODULES
Program C
Photometric Readout Digital display of A, %T and C on screen Cell program for up to 4, 6 or 8 cells
Photometric Accuracy ±.004A at 1A
Program W
Photometric Noise Less than ±0.0001A at 550nm, 2nm b/width Wavelength program for up to 10
Baseline Stored in non-volatile memory wavelengths and time interval
Baseline Flatness ±0.001A (300nm/min, 200-1100nm) 2nm
bandwidth Quant M
Baseline Stability Better than ±0.0001A/hour Multi-wavelength assays at 2 or 3
wavelengths, Warburg and Christian
Computer Interface Bi-directional RS 232 c assays at 2 or 3 wavelengths, wine/colour
Scan Speeds 1-4000nm/min assay at up to 10 wavelengths
Wavelength Scale Expansions 1-100nm/cm Quant P
Method Storage 30 methods may be stored and retrieved Protein assays using Lowry, Bradford,
Method Protection Each protected by 4 digit passcode Biuret, BCA and direct UV
Reaction Kinetics With plots, reprocessing of data as Quant S
required, for single cell or up to 6 cells Quantification of corrected bands,
difference spectra, spectral stripping
Overlaid Scans Up to 8 scans may be overlaid
Cell Programming Up to 4, 6 or 8 cells Kinetics
Programme Cycles From 1 to 9999 Kinetic measurements with plots and
reprocessing of data
Programme Delay (cycle time) From 0 seconds to 4 days (1 sec resolution)
Wavelength Programming Up to 10 wavelengths. May be combined Kinetics M
Multi-cell Kinetics linked with
with a cell programme autochanging of cells and plotting of
Dynamic Scan Recall Last 25 scans automatically stored on board reaction curves
Storage of Spectra 100 spectra may be stored code protected
Formulae
Concentration Curve Fitting Linear, quadratic or cubic with editing of Construction and entry of mathematical
standards. 30 curves may be stored formulae with multiple wavelengths and
cells; automatic computation of results
Multi-language Operation Six languages provided
Formula Entry and Computation Complex formulae may be computed Validation
Batch Sampling Up to 100 samples under internal control Validation of absorbance, wavelength,
bandwidth, stray light etc. using liquid
Date and Time Information Printed from real time clock samples or certified standards
Power Requirements 110-250V, 50/60Hz, 170 watts.
DataStream
Dimensions and Weight 635 x 410 x 210mm; 33kg Data transfer to, or control by a PC.
Graphical presentation, transfer of data to
Cecil Instruments policy is one of continuous development. We therefore reserve the right to change specification without notice. Excel, Word etc.
CECIL INSTRUMENTS LIMITED MILTON TECHNICAL CENTRE CAMBRIDGE CB24 6AZ ENGLAND ISO 9001
TEL: 01223 420821 FAX: 01223 420475 E-MAIL: info@cecilinstruments.com WEB SITE: www.cecilinstruments.com CERTIFIED COMPANY
12
ULTRA FAST SCANNING
UV-VISIBLE DETECTOR
1
Universal UV/Visible Detector
WaveQuest the ultra fast scanning Dynamic Absorbance
Detector (DAD) is a major advance in UV-Visible detector
design, offering the capabilities of a Photo Diode Array
(PDA) detector with greatly improved performance when
used for chromatogram monitoring.
Very high speed scanning of eluting peaks, “on-the-fly”,

makes purity checks, the determination of optimum
monitoring wavelengths for unfamiliar peaks (lambda
max scans), and identification of peaks using library
spectra all possible, with considerable cost saving.
New Super Fast Performance

CE4300 Dynamic Absorbance Detector
WaveQuest the CE4300 Dynamic Absorbance Detector
(DAD), is an ultra high speed scanning powerful WaveQuest the new CE4300 Dynamic Absorbance
alternative to Photo Diode Array (PDA) detectors. HPLC Detector for ultra fast scanning, part of the Adept HPLC
method development, purity determination, R & D and range from Cecil Instruments, offers all the spectral
Variable Wavelength Monitoring are all possible to the facilities of Photo Diode Array detectors, with better
highest quality. Dual wavelength monitoring and noise and drift performance, particularly important when
wavelength programming are also provided. used to monitor and quantify chromatograms.
The design is based on a double beam The CE4300 has a double beam optical system in which
spectrophotometer concept, with ultra fast wavelength the dispersed radiation is dynamically scanned across
scanning over the range 190-700nm. the detectors, in the manner of a UV-Visible double beam
spectrophotometer, at ultra fast speeds – up to
This dynamic scanning provides UV spectra in only 1000nm/second.
200ms, previously only possible using the static optical
system of a PDA detector. All components of the detector – monochromator, optical
system, lamp optics, detector optics and signal
Powerful signal processing software has been developed
processing software have been carefully optimised to
which enables very high quality “on-the-fly” scans to be
provide unprecedented performance in both wavelength
triggered by eluting peaks without peak degradation.
scanning and single or multi-wavelength monitoring.
Spectral data is transferred by Cecil’s DataStream
software to a PC workstation, where the same software The lower noise level when monitoring a chromatogram
offers spectral manipulation and processing, library makes accurate measurements at the highest
facilities and the export of data to applications such as sensitivities possible when measuring very low
Excel, Word etc. All data may be stored on disk. concentrations.
Eluting peaks may be integrated at the same time as The lower drift, as much as 20 times lower, gives flat
purity and peak identification is carried out. Quantitative chromatogram baselines making integration of peaks
evaluation and reporting is carried out by Cecil’s CE4900 more accurate, particularly at high sensitivities.
PowerStream software, which is combined with
DataStream software. Taper Beam Optics
Taper beam optical imaging at the flowcell, with all
One Detector Covers All UV Applications transmitted energy reaching the detector, provides
maximum energy collection. The result is minimal
A single beam Photodiode Array Detector (PDA), used for
baseline wander due to changes in the solvent refractive
method development and R & D, will often be replaced by
index during gradient formation.
a dual beam UV variable wavelength detector (VWD), for
routine monitoring, when the developed method is
adopted, because of the poor drift performance of single Performance Validation
beam PDA detectors. Their performance can be as much
as 20 times worse. WaveQuest has a double beam optical Validation of detector performance is carried out
system with very high all round performance and the conveniently, using the instruments standard software.
lowest drift available. A Holmium Oxide filter is automatically inserted in the
optical path when the calibration routine is selected.
The CE4300 is used as a high performance UV detector for
variable wavelength monitoring. WaveQuest, which is Bandwidth validation is automatic, using the Deuterium
less expensive than a PDA detector, therefore has a emission line at 656.1nm. Absorbance accuracy is
double cost advantage. checked using filters traceable to NPL standards.
2
PowerStream Software – CE4900 New Advanced Design
PowerStream is a chromatography control and data The design of the WaveQuest, CE4300, Dynamic
processing software and interface developed by Cecil Absorbance Detector is based on a double beam UV/Visible
Instruments to be comprehensive and convenient to use Wavelength Scanning Spectrophotometer concept, with an
from a single PC workstation. It provides powerful data optical system which also provides the highest
handling and system control for isocratic, binary and performance as a dual wavelength, programmable detector.
ternary HPLC systems.
The 4nm optical bandwidth, constant over the 190-700nm
DataStream software provides sophisticated spectral range, provides high quality spectral scans for easy
processing. Spectral data is transferred from the detector identification of unknown components. The digital signal
to the PC where it is graphically displayed, manipulated processing and optical and mechanical systems provide
and exported as required. ultra fast scanning and the best noise and drift
performance available. Accurate measurements at high
Chromatographic peaks are automatically scanned to sensitivities are therefore possible.
determine purity, identify components, and determine
optimum wavelengths for chromatogram monitoring – Ultra Fast Scans of Narrow Peaks
Lambda max scans. Multiple scans of peaks enable
checks to be made of peak homogeneity. The ultra high speed scanning of the WaveQuest is
shown. A very fast binary separation was carried out, on
Wavelength scans are compared with your own library to a Cecil gradient elution system 4S, of sixteen
establish the identity of unknown components. A spectral polyaromatic hydrocarbons, designated by the American
library search facility is provided. Environmental Protection Agency as priority pollutants.
The time for the elution was less than 4 minutes, using a
Visual comparison of spectra is valuable for identifying 5cm column with a 3 micron packing.
components and for comparing scans made for purity
determination. To further assist assessment, a Several of the peaks have widths at half height less than
mathematical calculation is provided, which produces a 1.5 seconds. The spectra of all peaks were displayed in
figure indicating the degree of match or similarity. real time and are shown here overlayed in groups of four.
3
Peak Impurities Revealed Dual High Performance
The outstanding performance of the WaveQuest is When a method has been developed using the CE4300
illustrated by a number of chromatographic Dynamic Absorbance Detector, the detector may be
applications described in this brochure. used as a sensitive and very stable monitor for the
application, with performance much superior to that of
The application shown here is a chromatogram of a a Photo Diode Array detector.
mixture, obtained under isocratic conditions. It
appears to be a simple three component mixture. Should the CE4300 be required for further method
development or R & D it may be replaced by the
Peak purity checks were made for each of the peaks CE4200 Variable Wavelength Monitor. This is a top
with ultra-fast “on-the-fly” scans on both the performance detector with superb noise and drift and
ascending and descending sides of each peak. is therefore recommended for detection at the very
highest sensitivities. Excellent linearity allows
Peaks 1 and 2 gave identical spectral scans but Peak 3 accurate quantification of samples at very low or high,
gave totally different spectra between the “up side” concentrations.
and “down side” scans indicating a major peak
impurity, giving rise to the scan shown in red. WaveQuest offers better sensitivity and lower
detection limits than a Photo Diode Array detector.
Despite the presence of the impurity, peak 3 appears
visually symmetric and indeed the asymmetry factor The WaveQuest represents two instruments in one, a
calculated for the peak was close to 1.00, giving no scanning spectrophotometer and a variable
indication of the presence of an impurity. wavelength detector, resulting in major cost saving.
4
Lambda Max Scans
The same trial mixture as before, which separated into
three peaks under isocratic conditions, separated into four
peaks under binary gradient conditions using an Adept
System 4S.
Each peak was scanned at its maximum - Lambda max scan

– and the spectra displayed whilst the chromatogram was
simultaneously displayed and quantified by integration to
provide baseline corrected peak areas and peak heights.
Peaks 1, 2 and 4 are quantitatively similar and all have peak

maxima close to 222 and 273nm.
Peak 3 scan is clearly very different having maxima near

234, 268, 316, and 331nm.
Optimum monitoring wavelengths for each peak may be

chosen from these scans.
The photo shows an Adept Isocratic System 2S. The

WaveQuest detector, fitted with a column and injector valve
panel, is mounted on the CE4100 pump, with the
PowerStream interface unit at the bottom of the stack.
An optional column oven is fitted on the right. The

system offers Diode Array performance and the very best
dual and variable wavelength monitoring, with
exceptional compactness, as does the Adept Gradient
System 4.
5
Normalised Overlays Confirm Purity
Automatic peak purity checks were made by the same
upside and downside, “on-the-fly”, scans for all four
peaks.
The results are shown for peaks 3 and 4 only, overlayed

before and after normalisation of the spectral scans. Peak
4 is shown in the upper two plots and Peak 3 in the lower
two.
From the overlays, particularly when normalised, it is

seen that a near perfect match is obtained indicating that
these peaks were homogenous and hence pure, as also
were Peaks 1 and 2.
Eight Scans of Peaks

Several means of triggering the wavelength scan of a
peak are user selectable.
In the example shown here, WaveQuest was

programmed to make repetitive scans of pyrene as it
eluted.
The use of the cursor is demonstrated by using it to

measure the absorbance maximum at 326.7nm. The drop
line shows that all the scans have the same wavelength
for this absorption maxim.
6
Spectral Overlay Confirms Identity
A different mixture of the same components was used to
produce the chromatogram shown here with its 4 peaks.
Spectral library searches were made in order to identify

the components, in conjunction with retention time data.
Peaks 1, 2 and 4 can be identified as alkyl phthalates, most

probably dimethyl, dibutyl and di(ethylhexyl) phthalates.
ADVANCED DESIGN Peak 3 is identified as pyrene. The library reference

spectrum of pyrene overlayed on this impurity peak
● 190-700nm shows an almost perfect fit, and together with the
● Ultra fast “on-the-fly” scans retention time data for pyrene provides confirmation of
identity.
● UV scan in 200 ms
● Double beam
● 4nm optical bandpass
● 1nm digital resolution
● Superb stability
● Fast warmup
7
Analysis of Sugar Free Soft Drinks
The new ultra fast scanning detector from Cecil
Instruments, the WaveQuest, was used as part of an
Adept System 4S to separate, identify and quantify the
components of soft drinks.
Using the CE4300, high quality UV-Visible spectra were

obtained “on-the-fly” without the use of an expensive
Photo Diode Array detector. VERSATILITY
The spectra indicate the most appropriate wavelength for ● Highest sensitivity VWD
monitoring any given material. The spectra together with
retention time data are sufficient to provide positive ● Very low noise
identification of eluted peaks.
● Very low drift
The chromatographic separation of commonly occurring
● Method storage
components of soft drinks and fruit juices is shown. The
gradient elution used a C18 column, with a phosphate ● Dual wavelength operation
buffer: methanol binary gradient, monitored at 210nm as
some of the components lack chromophores at longer ● Programmed monitoring of 6 wavelengths
wavelengths.
● Simultaneous 4 wavelength monitoring
The peaks in the chromatogram were scanned “on-the-
fly” during elution and the spectra displayed in adjacent
windows.
Four scans are shown here, overlayed. Sufficient

information is available to identify a component peak
when taken in conjunction with the retention time.
8
Library Search Yields Identification BENEFITS
The use of the Spectral Library for peak identification is
● Spectral Storage
demonstrated by the chromatogram shown, which was ● Simple intuitive menu operation
obtained from a low cost “lemonade” style soft drink.
● Stackable with Adept HPLC modules
Comparisons were made between the UV spectra of the ● Flowcell replacement without adjustment
eluted peaks, with those of reference spectra which had
been previously scanned and stored in the Spectral ● Validation software
Library. ● Spectral library
Overlay of these scans after normalisation indicated that ● Library search
these peaks were identical with Citric Acid (Peak 3),
Saccharin (Peak 5), Aspartame (Peak 7) and Benzoic Acid
(Peak 9).
Retention times for the major components coincided with

those of the library spectra confirming the identifications.
The scan of Peak 9 is shown overlayed with the Library

Spectrum of Benzoic Acid displayed in the upper window.
The very high quality of the spectral match obtained, is
clearly seen.
A statistical calculation is programmed in the DataStream

software, enabling a match index to be calculated
between spectra, for identification and also purity.
9
Spectral Match Index
To confirm the identity of the sixteen separated peaks
shown in the fast separation, known reference standards
were injected and eluted under the same conditions as
the sample mixture.
Comparison of spectra and retention times allows

positive identification of all the components.
The screen display shown here illustrates the process,

showing a reference library spectrum, which is overlayed
on peak 8 in the chromatogram of the sixteen component
mixture.
Applying the mathematical routine for spectral match, or

similarity, to the data for the sample and library spectra
shown here, produced a match index of 996.5, which is
displayed on the screen.
The photograph shows the WaveQuest detector
A figure greater than 990 indicates a good degree of fitted with the column mounting panel and cover. A
match, and a figure above about 900 indicates some Rheodyne injection valve is also fitted to the panel
degree of match.
The arrangement provides close coupling between
A figure less than 100 indicates very little match, or the column and flow cell, for minimum dead
similarity between the scans. volume.
10
Excellent Analytical
Precision
The excellent precision of the
WaveQuest, and indeed the
complete Adept Gradient System
4S, is demonstrated by 7 repeat
Component Summary
injections and elutions of the soft
drink shown here in overlay mode,
each chromatogram being offset
by 50mA.
The phosphate buffer: methanol

baseline was first recorded and is
shown.
Statistical analysis of some of

these results is shown in summary
report comment.
The report shows a % RSD for the

peak caffeine of 0.33.
11
Scanning Specification Ordering
Wavelength Range 190-700nm CE4300 Dynamic Absorbance Detector
Optical Bandwidth 4nm – constant Wavelength range 190-700nm, 4nm constant optical
Digital Resolution 1nm bandpass, ultra fast wavelength scan (on-the-fly)
Wavelength Accuracy 1nm with auto peak triggering.
Wavelength Reproducibility ± 0.1nm Variable wavelength monitoring, dual wavelength
operation and programmable monitoring for up to
Absorbance Range 0.001 to 3A
6 wavelengths.
Scan Speeds Up to 1000nm/s – UV scan in 200ms Complete with instruction manual, power cable,
Baseline Flatness ± 0.002A over most of range signal cable and air test cell.
Second Order Filter Fast automatic insertion at 370nm Requires flowcell to be ordered.
Noise < ± 0.5 x 10–5 A, 230nm, 2 s T/C, using air test cell
Drift < ±0.3 x 10–4 A/h, 230nm, at equilibrium using air test cell CE4051
Overlayed Scans Up to 8 scans may be overlayed Column mounting panel with column cover.
Scan Trigger Start of peak, set Abs value, point of inflection or at peak
Number of Peak Scans Up to 100 peaks for a single elution CE4050
Scan Storage Up to 100 scans password protected Column mounting panel with column cover and
Rheodyne injection valve fitted with 20µL loop.
Multiple Scans of a Peak Up to 8 scans of any peak
Used with DataStream Data to PC, PC control, scan triggering, Lambda max. Flowcells
Software scans, peak purity and purity calculation, graphical
presentation, overlay, normalisation, disk storage, library 1200 07 70 8µL x 10mm pathlength
store and search, export, and many other functions. 1200 07 71 30µL x 10mm pathlength
1200 07 72 4µL x 5mm pathlength
1200 07 73 1mm pathlength preparative flowcell
Variable Wavelength Detection Specification 1200 07 74 12µL x 7mm pathlength
Other flowcells available on request
Wavelength Range 190-700nm
Wavelength Accuracy 1nm Printers
Wavelength Reproducibility ± 0.1nm
Absorbance Range 0.001 to 3A 8000 70 01 Dot matrix printer with signal cable
Absorbance Linearity Better than 1% 0.001 – 2A 8000 72 01 Colour inkjet printer with signal cable
Optical Bandwidth 4nm – constant 8000 73 01 Laser printer with signal cable
Dual Wavelength Operation Simultaneous measurement at 2 wavelengths and ratio CE4900
Wavelength Programming Measurements at up to 6 different wavelengths with a PowerStream software – provides PC workstation
time program and selectable sensitivity control of Cecil HPLC systems and data processing
Noise ± 0.5 x 10–5 A at 230nm, 2s T/C, using air test cell of chromatograms.
Drift ±0.3 x 10–4 A/h at 230nm at equilibrium, using test cell 9000 20 00
Noise – Dual Wavelength ± 3 x 10–5 A at 250/280nm DataStream Software - required for operation of the
Drift – Dual Wavelength ± 3 x 10–4 A/h at 250/280nm, constant temperature CE4300 in conjunction with a PC. No data
processing of chromatograms.
General Specification
Optical System Double Beam
Monochromator Littrow with 1200 l/mm holographic grating
Straylight < 0.02% at 220nm and 340nm
Detectors Two Silicon Diodes
Lamp Deuterium lamp with elapsed time indicator
Taper Beam Optics All energy collected to minimise RI effects
Self Test Automatic at switch-on; continuous monitoring of key
functions
Autozero By control panel or external trigger
Time Constant 0.1 second – 10 seconds, selectable System Requirements
Wavelength Calibration Automatic at switch-on using deuterium emission line.
Display Four-line display of parameters, menus, prompts, ● Computer with Pentium class processor
wavelength, absorbance etc. ● Pentium 200 MHz or better
Fast Number Entry By use of special number keys ● Microsoft Windows XP
Event Marking From keypad or by external trigger ● Microsoft Windows 2000
Method Storage For up to 30 methods, security protected ● 128 MB of RAM or more
Automatic Power-Up/Off Pre-programmed to save warm up time ● Hard-disk space required: 15 MB
Outputs Two absorbance outputs, or absorbance and absorbance ● CD-ROM drive
ratio, parallel printer, data available to PC. ● XGA or higher resolution monitor
Inputs Event, autozero ● Monitor resolution 1024 x 768 or better
Size and Weight 365 x 290 x 140mm, 17kg
● Microsoft Mouse or compatible pointer
Power Requirements 115V or 230V, 50/60Hz, 50VA
ISO 9001: 2000
TELEPHONE: 01223 420821 FAX: 01223 420475
CERTIFIED COMPANY
12
Dedicated to Water Analysis Programmed Methods
The new AquaQuest Series 4000 spectrophotometers have All instruments are completely programmed for approximately
been specially designed to cover the complete requirements 170 water analysis methods based on the proven range of
of water analysis spectrophotometers. Both visible range and Merck 'Spectroquant' test kits.
UV and visible range instruments are offered, each with or
without an integral printer for convenient presentation of Users may in addition develop their own methods and
results without the extra space required by an external calibrations to be stored in security protected memory for
printer. instant recall and use at any time.
Versatility Speed of Operation

Whatever special needs your laboratory may have, the Choosing a test method complete with the appropriate
AquaQuest Series 4000 instruments are likely to offer the sample pathlength could not be simpler, taking only about 15
flexibility, power and performance required. Wavelength seconds for selection and the instrument to be set up ready
scanning, cell programming, batch sampling, wavelength for the measurement. Quick change sample holders each
programming, time course plotting and reaction Kinetics are accommodating more than one sample pathlength further
all available. adds to convenience and speeds up operation.
Performance Software Enhancement by E-SEF

Superb performance is the result of the excellent E-SEF - encoded software enhancement facility - allows
specification of the AquaQuest Series 4000. High stability, optional software modules to be enabled by entering
low straylight, low noise and high wavelength setting encoded numbers. These numbers can be provided by
precision ensure that reliable and accurate measurements telephone, fax or E-mail. Software requirements may be
may be made to 3 Absorbance units. tailored after instrument purchase without incurring delay.
2
000
LARGE SCREEN : COMPACT PRINTING
Informative Graphic Display USER BENEFITS
SUPERB OPERATIONAL SIMPLICITY
Menus, scans, curves and tabulated Pre-programmed Test
results are displayed on the large backlit
LCD display with contrast control. Lists
may be scrolled vertically and plots,
Merck Spectroquant Kits
WIDEST APPLICATION RANGE scans and data horizontally to give an
effective 430mm screen width. Shown User Method Storage
below is a typical wavelength scan which
may be fully viewed by scrolling, a Wavelength Scanning
SIPETTE AND AUTOMATED SAMPLING
printout of the same scan is shown
below. Operation in six languages is
provided for operator convenience. Time Plots
PROGRAMMED SAMPLING Curve and Line Fit

Integral Printer
Kinetics
PREPROGRAMMED ANALYSES
The integral printer may be used to
provide a hard copy of all experimental Large Backlit Screen
results. Time course plots, wavelength
scans and calibration curves may also be Integral Printer
plotted with full documentation, timed
FOR MERCK SPECTROQUANT TESTSand dated for GLP. The printer is of the
dot matrix type and does not require E-SEF Software Call-Up
expensive heat sensitive paper. A typical
printout of results is shown on the left. Only 48cms Wide
PERFORMANCE
Accurate to 3A
Wavelength Setting 0.1nm
Stability ±0.001A/H
Straylight 0.01%
Validation Software
Traceable Standards
3
LARGE SCREEN : COMPACT PRINTING
SUPERB OPERATIONAL SIMPLICITY APPLICATIONS

Waste Water
WIDEST APPLICATION RANGE Boiler Water
Cooling Water
SIPETTE AND AUTOMATED SAMPLING Drinking Water
River Water
PROGRAMMED SAMPLING Beverages
Foodstuffs
PREPROGRAMMED ANALYSES Chemicals
Surface Finishing
FOR MERCK SPECTROQUANT TESTS Agriculture
Rapid Operation Convenient Sampling
Paint
Simply enter the quantitative mode Insert the 16mm tube or 10mm cell in
and follow the menus displayed on the the dual sample holder, press the 'Run' Paper
screen, selecting pathlength and the key and the result is displayed and
required analysis each at the touch of printed as required. Other holders and Pharmaceuticals
a key. The instrument is then set ready sampling techniques may be used as
for the analysis selected. required. Hydroponics
4
SUPERB OPERATIONAL SIMPLICITY
000
WIDEST APPLICATION RANGE
Curve and Line Fitting
SIPETTE AND AUTOMATEDAfitted
SAMPLING
straight line, quadratic or cubic curve may be automatically
to a set of up to 30 standards. Fits are possible either with
an intercept or forced through zero. All entries are prompted for
simplicity of operation. With the possibility of using such a large
PROGRAMMED SAMPLING number of standards, duplicate or multiple standards may be

used for any given concentration. A quadratic curve is shown
here.
Editing and Storage

Any suspect standards observed by inspection of the screen
display may be deleted or replaced and a new fit produced.
FOR MERCK SPECTROQUANT TESTS

Up to 30 curves may be stored, security protected, for future
recall and processing.
Time Course Plotting

The course of any reaction with time may be followed by simply
entering the mode, selecting the wavelength and entering the
duration of the plot, scale and absorbance range as prompted.
The data is displayed on screen or plotted and may be

reprocessed and stored security protected for future use.
A time plot on the screen is shown on the left. A printout is by

pressing the RUN key, or may be made in real time.
Reaction Kinetics
Reaction Kinetics is available with reaction plots to screen and
printer. Data may be reprocessed as required. Regression
analysis is applied to the user selected portion of the reaction
plot and results are reported in Units/Litre.
Sampling Flexibility
Some of the complete range of sampling accessories are
shown. All instruments are fitted as standard with a
special dual sample holder designed to accommodate both
10mm rectangular cuvettes and 16mm reaction tubes as
supplied with many of the Merck test kits; time is saved as
no change of holder is required when changing between
the two.
A holder for 10, 20 and 50mm cells is also available as are

holders for rectangular or cylindrical cells, up to 100mm in
length.
Rapid Change Sample Holders

Sample holders take only seconds to change without the
use of any tool. They are retained by two pillar thumb
screws, and require no adjustment after fitting saving
valuable time.
5
Dedicated with speed and
convenience for Water Analysis.
Designed also for use in a very

wide range of UV/Visible
applications.
WIDEST APPLICATION RANGE

Sipette System
PROGRAMMED SAMPLING
The sipette system shown in the photograph below
may be used to advantage when a number of
samples have to be measured in sequence. All
measurements are made in the same cell improving
accuracy while cell handling and breakage are
avoided. The Sipette pump mounts inside the sample
compartment and operates under control of the
spectrophotometer microprocessor system. Samples
after measurement may be returned and saved, or
pumped to waste.
Multi-Cell Sipette System

A cell changer designed to accommodate three cells,
of 10mm, 20mm and 50mm pathlengths, connected
in series, speeds up the change between cells of
different pathlengths when using a sipette system.
This avoids cell handling and breakage.
Batch Sampling
Where large numbers of similar samples are to be
measured, a batch sampler is available for up to 100
samples. The pump for the system is sample
compartment mounted and under the instruments
internal system control.
8
000
PROGRAMMED SAMPLING
Automatic Cell Changers

For automatic measurements cell changers are available for
4, 6 or 8 cells and may be fully programmed for
measurements.
A wavelength programme for up to 10 wavelengths is also

available and may be used with a single cell or combined with
a cell changer for a cell and wavelength program.
The sample changers are shown above together with a

photograph of the 6 cell changer fitted to a
spectrophotometer.
Ordering Accessories
SAMPLE HOLDERS Part No. THERMOSTATTIIMG
Single cell holder 1010 31 00 Water flow holder for auto 4 cell changer 2020 36 15
Dual holder for 10mm cells and 16mm tubes 4002 32 00 Water flow holder for single, sipette cell 1010 33 00
Holder for 10, 20 and 50mm cells 2010 38 00 Peltier controller and 10mm cell holder 2020 31 00
Universal cell holder, 10mm cells upwards 1010 38 00 Peltier controller and 4 cell auto changer 2021 32 00
Long pathlength cylindrical cell holder 1010 39 00 Cell compartment lid and pipe port for water
CELL CHANGERS AND HOLDERS thermostatting 1010 05 00
4 cell automatic changer (no holder) 2021 34 00 PRINTERS, CABLES AND COMPUTERS
6 cell automatic changer and holder 2021 36 00 RS232c cable for PC with 25 pin outlet and
8 cell auto changer and holder 4000 37 00 protocol manual 2021 26 00
3 x (1-50mm) cell changer for water analysis 2020 35 00 RS232c cable for PC with 9 pin outlet and
4 x 10mm cell holder for auto changer 2010 24 00 protocol manual 2021 83 00
4 x 50mm holder for auto changer 2010 36 00 Printer connection cable 8000 71 00
SIPETTE, BATCH AND SIP SAMPLING Dot matrix printer 8000 70 01
Holder for 10mm sipette cell 1010 32 00 Ink jet printer 8000 72 01
Holder for sipette cells up to 50mm 2010 38 00 Print rolls and ribbon for integral printer 2020 03 20
Batch sampler for 40 samples with pump 2020 82 00 CALIBRATION FILTERS
Batch sampler for 100 samples with pump 2021 82 02 Holmium filter in holder 202 01 44
Return sipette system no cell or holder 2021 21 00 Didymium filter in holder 303 01 30
LAMPS Set of 4 certified absorbance filters in holders 594 44 00
Deuterium lamp with hours indicator 2202 01 42 Set of 6 certified absorbance filters in holders 594 66 00
Tungsten halide lamp - in pairs 2303 01 40 Set of 2 certified wavelength filters in holders 303 40 00
9
PROGRAMMED SAMPLING
ANALYTE RANGE UNIT pl (mm) ANALYTE RANGE UNIT pl (mm)
Aluminium 0.05-0.35 mg/L AI 50

FOR MERCK
COD
SPECTROQUANT
10-150 mg/L COD 16
TES
Aluminium 0.20-1.50 mg/L AI 10 COD 100-1500 mg/L COD 16
Aluminium 2.0-13.0 mmol/m AI 3
50 COD 500-10000 mg/L COD 16
Aluminium 7.4-55.6 mmol/m AI 3
10 COD 50-500 mg/L COD 16
Ammonium 0.010-0.620 mg/L NH4-N 50 COD 300-3500 mg/L COD 16
Ammonium 0.010-0.800 mg/L NH4 50 Copper 0.05-2.00 mg/L Cu 50
Ammonium 0.10-2.70 mg/L NH4-N 10 Copper 0.10-8.00 mg/L Cu 16
Ammonium 0.10-3.50 mg/L NH4 10 Copper 0.20-6.00 mg/L Cu 10
Ammonium 0.20-10.00 mg/L NH4 16 Copper 0.8-31.5 mmol/m3 Cu 50
Ammonium 0.20-7.80 mg/L NH4-N 16 Copper 3-126 mmol/m3 Cu 10
Ammonium 0.6-44.4 mmol/m3 NH4 50 Cyanide 0.002-0.100 mg/L CN 50
Ammonium 0.8-21.7 mg/L NH4-N 16 Cyanide 0.025-0.500 mg/L CN 10
Ammonium 1.0-28.0 mg/L NH4 16 Cyanide 0.025-0.500 mg/L CN 16
Ammonium 4.0-78.0 mg/L NH4-N 16 Fluoride 0.025-0.500 mg/L F 50
Ammonium 5-100 mg/L NH4 16 Fluoride 0.10-1.50 mg/L F 16
Ammonium 6-195 mmol/m NH4 3
10 Formaldehyde 0.025-2.250 mg/L HCHO 50
Ammonium 60-1550 mmol/m NH4 3
16 Formaldehyde 0.1-10.0 mg/L HCHO 16
Boron 0.050-0.800 mg/L B 10 Formaldehyde 0.02-1.80 mg/L HCHO 50
Cadmium 0.005-0.300 mg/L Cd 50 Formaldehyde 1.00-9.00 mg/L HCHO 10
Cadmium 0.025-1.000 mg/L Cd 16 Gold 0.5-12.0 mg/L Au 10
Calcium 1.0-15.0 mg/L Ca 20 Hardness Res 0.50-5.00 mg/L Ca 16
Calcium 5-160 mg/L Ca 20 Hardness Res 0.075-0.750 deg. Hardness 16
Calcium 7-224 mg/L CaO 20 Hardness Tot. 0.7-22.0 deg. Hardness 16
Calcium 13-4000 mmol/m CaCO3
3
20 Hardness Tot. 5-140 mg/L Ca 16
Chloride 0.28-4.23 mol/m Cl
3
10 Hydrazine 0.02-1.00 mg/L N2H4 50
Chloride 1.0-20.0 mg/L Cl 20 Hydrazine 0.20-5.00 mg/L N2H4 10
Chloride 10.0-150 mg/L Cl 10 Hydrazine 0.6-31.2 mmol/m3 N2H4 50
Chloride 28-564 mmol/m3 Cl 20 Hydrazine 6-156 mmol/m3 N2H4 10
Chloride 5-125 mg/L Cl 16 H peroxide 2.0-20.0 mg/L H2O2 16
Chlorine 0.05-1.50 mg/L Cl2 50 Iron 0.01-1.00 mg/L Fe 50
Chlorine 0.05-1.50 mg/L Cl2 50 Iron 0.2-18.0 mmol/m Fe 3
50
Cl dioxide 0.02-1.00 mg/L CIO2 50 Iron 3.6-45.0 mmol/m Fe 3
20
Cl dioxide 0.05-1.50 mg/L ClO2 50 Iron ll/lll 0.2-20.0 mg/L Fe 10
Cl dioxide 0.50-5.00 mg/L CIO2 10 Lead 0.02-1.50 mg/L Pb 50
Chromium 0.010-0.600 mg/L Cr 50 Lead 0.10-5.00 mg/L Pb 16
Chromium 0.05-2.00 mg/L Cr 16 Magnesium 5.0-50.0 mg/L Hg 16
Chromium 0.10-3.00 mg/L Cr 10 Manganese 0.01-2.50 mg/L Mn 50
Chromium 0.2-11.5 mmol/m Cr 3
50 Manganese 0.2-46.0 mmol/m Mn 3
50
Chromium 2.0-58.0 mmol/m Cr 3
10 Manganese 0.5-10.0 mg/L Mn 10
COD 4.0-40.0 mg/L COD 16 Manganese 9-182 mmol/m Mn 3
10
10
000
ANALYTE RANGE UNIT pl (mm) ANALYTE RANGE UNIT pl (mm)
Nickel 0.05-2.00 mg/L Ni 50 Phos(PMB) 0.2-11.5 mg/L P3O5 10

Nickel 0.10-6.00 mg/L Ni 16 Phos(PMB) 0.2-15.3 mg/L PO4 16
Nickel 0.20-5.00 mg/L Ni 20 Phos(PMB) 0.3-15.3 mg/L PO4 10
Nickel 0.4-34.0 mmol/m Ni 3
50 Phos(PMB) 0.6-32.3 mmol/m PO4 3
50
Nickel 3.0-85.0 mmol/m Ni 3
20 Phos(PMB) 1.0-25.0 mg/L PO4-P 16
Nitrate 0.11-3.40 mg/L NO3-N 16 Phos(PMB) 2-160 mmol/m PO4 3
16
Nitrate 0.20-5.60 mg/L NO3-N 50 Phos(PMB) 3-161 mmol/m PO4 3
10
Nitrate 0.25-4.50 mg/L NO3-N 50 Phos(PMB) 3.1-76.8 mg/L PO4 16
Nitrate 0.5-15.0 mg/L NO3 16 Phos(VM) 0.5-25.0 mg/L PO4-P 16
Nitrate 0.5-18.0 mg/L NO3-N 16 Phos(VM) 1.0-30.0 mg/L PO4-P 10
Nitrate 1.0-25.0 mg/L NO3 50 Phos(VM) 1.1-57.0 mg/L P2O5 16
Nitrate 1.1-20.3 mg/L NO3-N 10 Phos(VM) 1.5-77.0 mg/L PO4 16
Nitrate 1.1-22.6 mg/L NO3-N 16 Phos(VM) 2.0-69.0 mg/L P2O5 10
Nitrate 2.0-80.0 mg/L NO3 16 Phos(VM) 3.0-92.0 mg/L PO4 10
Nitrate 5.0-90.0 mg/L NO3 10 Phos(VM) 16-807 mmol/m PO4 3
16
Nitrate 5-100 mg/L NO3 16 Phos(VM) 32-970 mmol/m PO4 3
10
Nitrate 8-242 mmol/m NO33
16 Potassium 5.0-50.0 mg/LK 16
Nitrate 16-403 mmol/m3 NO3 50 Silicon 0.010-0.800 mg/L Si 50
Nitrate 32-1290 mmol/m3 NO3 16 Silicon 0.02-1.70 mg/L Si02 50
Nitrate 80-1450 mmol/m3 NO3 10 Silicon 0.10-5.00 mg/L Si 20
Nitrite 0.010-0.180 mg/L NO2-N 50 Silicon 0.2-10.7 mg/L Si02 20
Nitrite 0.02-1.00 mg/L NO2-N 10 Silicon 0.3-28.5 mmol/m SiO2 3
50
Nitrite 0.020-0.600 mg/L NO2 50 Silicon 3-178 mmol/m SiO2 3
20
Nitrite 0.020-0.610 mg/L NO2-N 16 Silver 0.10-1.00 mg/L Ag 50
Nitrite 0.05-2.00 mg/L NO2 16 Silver 0.50-5.00 mg/L Ag 10
Nitrite 0.05-3.00 mg/L NO2 10 Sulphate 0.21-2.50 mol/m SO4
3
16
Nitrite 0.4-13.0 mmol/m3 NO2 50 Sulphate 20-240 mg/L SO4 16
Nitrogen 0.5-18.0 mg/L N 16 Sulphide 0.020-0.500 mg/L S 50
Oxygen 0.5-12.0 mg/L O2 16 Sulphide 0.25-4.0 mg/L S 10
Ozone 0.05-1.00 mg/L O3 50 Sulphide 0.6-15.1 mmol/m S 3
50
Ozone 0.05-1.50 mg/L O3 50 Sulphide 7.6-121.0 mmol/m S 3
10
Ozone 0.50-5.00 mg/L O3 10 Sulphite 0.05-3.00 mg/L SO3 50
Phenol 0.025-1.500 mg/L PHE 50 Sulphite 1.0-25.0 mg/L SO2 16
Phenol 0.10-2.50 mg/L PHE 16 Surfactants 0.05-2.00 mg/L a-Ten 16
Phos(PMB) 0.01-11.5 mg/L P2O5 16 Tin 0.10-2.50 mg/L Sn 16
Phos(PMB) 0.02-1.00 mg/L PO4-P 50 Zinc 0.05-2.50 mg/L Zn 10
Phos(PMB) 0.05-2.30 mg/L P2O5 50 Zinc 0.050-0.500 mg/L Zn 50
Phos(PMB) 0.05-5.00 mg/L PO4-P 16 Zinc 0.20-5.00 mg/L Zn 16
Phos(PMB) 0.06-3.00 mg/L PO4 50 Zinc 0.8-38.2 mmol/m Zn 3
10
Phos(PMB) 0.10-5.00 mg/L PO4-P 10
11
SPECIFICATION ORDERING
Optical System Littrow monochromator using 1200L/mm Spectrophotometers are supplied

with dual cell holder, power cable
holographic grating; coated optics. and instruction manual
Accommodates 100mm cells
Optical Bandwidth 4nm, all versions VISIBLE RANGE

Wavelength Reproducibility Better than 0.1nm 325-1100nm Wavelength range 4nm
Bandpass
Wavelength Accuracy Within 1nm
Straylight Typically 0.01% at 220nm and 340nm - UV versions
325-1100nm Wavelength range 4nm
Photometric Ranges -0.3 to 3.0A, 0-200%T, 0-9999C Bandpass. With Integral Printer
Photometric Noise Less than ±0.0002A at 500nm
Photometric Accuracy 1% or 0.005A whichever is greater UV/VISIBLE RANGE

Absorbance Zero Stability Better than ±0.001A/Hr. at 500nm
Absorbance Zero Setting 0A and 100%T automatic by press button 190-1100nm Wavelength range 4nm
Bandpass
Filter and Lamp Selection Automatic - coupled to wavelength CE 4004 Spectrophotometer

190-1100nm Wavelength range 4nm
Display Screen - LCD High resolution backlit screen with contrast control, Bandpass. With Integral Printer
displays menus, curves, scans and all data etc. SYSTEM 1

Display Screen Scrolling Effective viewing width of 430mm Spectrophotometer, spare lamp(s)
spare fuses, 2 cells and dust cover.
Wavelength Selection Keyboard entry with GO TO key Example: CE4001 System 1 is a
CE4001 with spare tungsten halide
Reaction Kinetics Tabulation of results; reaction plots, reprocessing lamp, spare fuses, 2 glass cells and
Cell and Wavelength Programming Up to 8 cells and 10 wavelengths in any combination dust cover.
Peak Seek Automatic with baseline flattening
Curve and Line Fitting Linear, quadratic or cubic, with or without SOFTWARE MODULES
intercept, for up to 30 standards with curve editing
Program C
Wavelength Scanning Scan speeds up to 4000nm/min with the scan software Cell program for up to 4, 6 or 8 cells
Method Storage Up to 100 methods and 30 curves, security coded Program W

Wavelength program for up to 10
Sipette Sampling Under internal control with screen display measurements at a fixed wavelength
Batch Sampling For up to 100 samples under internal control Kinetics

Sample Temperature Displayed and set from Keyboard Kinetic measurements with or
without plots, reprocessing of data
Computer Interface Bi-directional serial RS232C and parallel ports
Scan
Sample compartment size 196 x 105 x 107mm (I x d x h) Wavelength scans with peak labelling
Power Requirements 110-250V, 50/60Hz, 170W/120W UV/Visible versions and peak tables. Time course plots
Weight 16.8Kg CE 4001, 18.6Kg CE 4002 Validation

Absorbance, wavelength and
17.7Kg CE 4003, 19.5Kg CE 4004 bandwidth validation using certified
Size 480 x 340 x 205mm (I x d x h) standards.
CECIL INSTRUMENTS LIMITED MILTON TECHNICAL CENTRE CAMBRIDGE CB24 6AZ ENGLAND ISO 9001
TEL: 01223 420821 FAX: 01223 420475 E-MAIL: info@cecilinstruments.com WEB SITE: www.cecilinstruments.com CERTIFIED COMPANY
12
COMPLETE ANALYTICAL SYSTEMS
Precision Temperature
Control
The eluent background conductivity

changes by about 2% for a 1ºC
change in temperature. The cell
temperature controlled enclosure
temperature may be set by the
operator to avoid ambient changes
causing drift. The default value is
set at 35ºC and controlled to better
than ±0.01ºC. Temperature
compensation is not therefore
required.
Pulse Free Pump
Mobile phase pumping is provided

by a dual piston, digitally
controlled, pump. Electronic
feedback control virtually
eliminates pulsing without using
mechanical damping.
Column Oven
The very precisely temperature
controlled oven accommodates
Anion and Cation columns and pre-
columns, as well as a background
suppressor cartridge with its over
pressure protection valve.
Close coupling to the detector

Superb Performance Ion Chromatography provides high stability, low noise
and low dispersion.
The completely new Ion Chromatography system from Cecil Instruments –
IonQuest – is a top performance modular system.
The conductivity cell is enclosed with a heat exchanger in a precisely

Leak Detector
thermostatted enclosure. The temperature of the enclosure is controlled to
better than ±0.01ºC. A specially designed leak detection
system is accommodated within the
The working temperature may be set by the operator to be between 25ºC cell oven enclosure.
and 50ºC and set with a default value of 35ºC during production testing.
The detection system may be used
to shutdown the system if a leak is
Modular Flexibility detected in the enclosure.
The system is easy to use by control panel operation, or by using the

sophisticated Cecil PowerStream software for data acquisition, processing
and reporting. Full system control is also provided by the workstation PowerStream Software
equipped with PowerStream.
This software, developed by Cecil
The modularity of the system enables its configuration to be changed with Instruments, provides system
ease, for instance to use a UV/Visible detector. control and superb data processing,
manipulation and reporting with
Modules may readily be replaced, to reduce down-time, should the need arise. truly unrivalled ease.
2
FOR ION CHROMATOGRAPHY
Detector – Advanced Design Chemically Suppressed Solvent
Background
The CE4710 Conductivity Detector is an entirely new
design from Cecil Instruments providing performance Sensitivity for the measurement of anions is greatly
of the very highest order due to its exceptionally low improved by reducing the conductivity of the eluent,
noise and unrivalled baseline stability. post column.
To achieve this standard of performance a very low An optional Anion Membrane Suppressor - SAMS ™ -
volume, 0.5µL, conductivity cell has been designed is available which is mounted in the column oven, in
and is operated in a precisely thermostatted series with and after the analytical column.
aluminium oven enclosure.
The suppressor may be used continuously and is
A specially designed, low dispersion, heat exchanger, regenerated by a Continuous Anion Regeneration
is also mounted in the cell enclosure preceding the System - CARS ™ - , which pumps the regeneration
detector cell. The exchanger stabilises the fluid through the post column suppressor cartridge.
temperature of the mobile phase entering the cell.
With the enclosed system controlled to better than The suppressed solvent background conductivity leads
±0.01ºC superb performance is always available. to reduced drift and noise and therefore higher
sensitivity for measurements is available.
Autozero and auto-range facilities are provided to
ensure convenience of operation. Built-in diagnostics Close Coupling to Oven
aids instrument evaluation and fault finding.
Close coupling of the detector to the oven is
The temperature controlled cell and heat exchanger important in eliminating the effects of ambient
enclosure is conveniently accessed from the side of the temperature fluctuation on results. The couplers can
detector module, as shown in the photograph. be seen on the side of the detector.
Detector Features
● Full control by PowerStream software workstation

● Keypad and LCD interface, for local operation, when The cell compartment thermostatted enclosure is
required shown in the lower photograph, with the side cover
removed for easy access.
● Large memory for analytical methods
● Autozero for operator convenience
● Operator selectable cell temperature; default 35ºC
● Leak detection system shutdown. Important for
unattended operation.
● Self diagnostics to confirm instrument function
● Mounting facility for Rheodyne injection valve
● Calculation of detector cell constant not required
● Polarity switching
3
DATA PROCESSING + CONTROL
Pump-Proven Performance
The Cecil CE4100 dual piston solvent delivery pump is

well established as very reliable, convenient to use,
with a very high performance specification.
The microprocessor controlled pump uses a precise
stepping motor drive for the dual overlapping pistons
which deliver solvent with very little pulsing.
Electronic feedback is used to control the pump speed
during its cycle to reduce pulsing to a negligible level.
No further mechanical pulse damping is required.
Delivery of mobile phase in the range 0.2 – 2.5mL per
minute, the usually used range, is well within the
ability of the pump, which delivers in the range
0.001mL/min to 10mL/min.
The wetted components of the pump are, sapphire,
The Total I C Concept ruby and ceramic inserts.
● Dual overlapping piston pump ● PowerStream data processing

● Microprocessor control for low pulsing ● PowerStream system control
● Chemically inert flow-path ● In line vacuum degassing
● Precisely thermostatted cell ● Regenerative chemical suppression
● Low dispersion heat exchanger ● IC using UV detector
● Dual column oven ● Suppressor cartridge in oven
● Leak detection ● Modular flexibility
Convenient Column Heater/Chiller (Oven)

It is important that the separation columns should operate at a
constant temperature, matching the temperature of the measuring cell.
The column oven module is a very convenient front

opening design accommodating two analytical columns and pre-
columns and also, when needed, a mobile phase background
conductivity suppressor cartridge and its over-pressure protection
valve.
The oven operates over the range from 5 ºC to 70 ºC

(maximum 12 ºC below ambient).
The default temperature is set at 35ºC.
The actual and set temperatures are displayed by a bright LCD

display and may be controlled by the adjacent keypad, or set by the
PowerStream software. Temperature stability is better than ±0.01ºC.
The flow tubes from the columns and suppressor cartridge enter and
leave the oven enclosure through convenient self-sealing ports. The
output port to the cell thermostatted enclosure lines up with the input
connector of the enclosure, when the modular system is arranged as
shown in the photographs of the system.
The very close coupling to the detector cell leaves only about 2
centimetres of tube between the oven and the cell. With the cell and
oven operated at the same temperature, say 35ºC, drift or noise due
to ambient fluctuations is eliminated.
A column switching valve may be fitted to the oven offering

convenient operation.
4
BY POWERSTREAM SOFTWARE
PowerStream – The Powerful Software PowerStream Features
All the data processing procedures and power you ● Digital and analogue outputs
need are elegantly available in PowerStream
software. Digital system control is also available. ● Process signals from multiple detectors
A typical display is shown here of a waste water ● Comprehensive integration parameters

cation analysis with a superimposed portion of the
chromatogram highlighting sodium. ● Calibration curves with unlimited number of
Detail and clarity are excellent and the software is standards
truly both easy to use and sophisticated.
● Linear, Quadratic and Cubic calibration curves
A fully interactive graphical interface provides for full ● Chromatogram overlays
method development, data capture and display of
chromatograms for live screen display during a run. ● Bracketing of samples
Developed to operate with Windows XP and VISTA, it
may also be used with Windows 7, 8 and 10. ● Digital control of a range of peripherals
5
FAST ACCURATE ANALYSES
Anions in Drinking Water
The Anion Suppressor was used

Column:- Allsep Anion 100
x 4.6mm
Mobile Phase:- 0.85 mmol/L
NaHCO3
0.90 mmol/L
Na 2CO3
Flow Rate:- 1.2mL/min

Injection Volume:- 20 µL
Column
Temperature:- 35ºC
Detector
Temperature:- 35ºC
Retention Time Anion Measured Some Applications

(mm : ss) concentration (ppm) Drinking, surface and waste water
02:27 Unknown -
Sulphites in wine
03:49 Chloride -
04:57 Unknown - Phosphoric and citric acids in soft
07:57 Nitrate 37 drinks
12:13 Phosphate - Organic acids in water
14:01 Sulphate -
Bromide in water
Nucleic acids
Fatty acids and hydroxy fatty acids
Amino acids
Dicarboxylic acids
Amino acids
Weak organic acids
Sodium in foods
Metals in soil
Azide and cyanate in explosives
Alkali metals and alkaline earths in
grain
Chromate and sulphate in electro
plating
Retention Cation Measured

Time concentration
Inorganic Cations in Effluent Water (mm : ss) (ppm)
Column:- Universal Cation 7 µm 100 x 4.6mm 01:54 Na + 72.03

Mobile Phase:- 5mmol Methanesulfonic acid 02:04 NH 4+ 29.39
Flow Rate:- 1.5 mL/min
02:34 K+ 36.30
Injection Volume:- 20 µL
Column and Detector 03:38 Mg 2+ 6.47
Temperatures:- 35ºC 4:17 Ca2+ 106.56
6
WITH HIGH SENSITIVITY
Phosphoric Acid in Soft Drinks
The established method for

determination of Phosphoric Acid in
cola beverages uses the
“molybdenum blue” analysis. This
spectrophotometric method is not
however rapid.
An Ion Chromatography method was

developed, in our applications
laboratory, which is both rapid and
accurate. The Cecil CE4715
suppressor was used to reduce
background conductivity. This
improved sensitivity with reduced
noise and drift. The reduction was
from about 500µS/cm to 20µS/cm.
The flow rate of the eluent: 2 mM

Na 2CO3 / NaHCO 3 buffer, was
1.3mL/min with a 7 micron anion
column.
The determination of Phosphoric acid in a cola drink, as shown here, The column oven and cell were both
required under 5 minutes using a flow rate of 1.3mL/min. at 45ºC.
Versatility
The modular design of the system

leads to complete versatility through
interchange of modules.
For methods requiring the use of a

UV/Visible detector, the conductivity
detector is easily replaced by the
CE4300 variable wavelength
detector, or the fast scanning DAD
WaveQuest detector as shown in the
photograph.
7
SPECIFICATIONS ORDERING
Conductivity Detector CE 4710 IonQuest System 1

Conductance Display Range 0.01 – 5000µS/cm CE 4100 pump, CE 4710 conductivity
Output Signal Selectable for anions or cations detector, conductivity flow cell, CE
Time Constant 0.1, 0.2, 0.5, 1, 1.5, 2, 5s 4601c column oven, CE 4033 solvent
Autozero Range Typically up to 10,000µS/cm bottle and flow pipe co-ordinator,
Synchronous Detection Frequency 5kHz rheodyne injector valve with 100µL
Noise Less than ±1nS/cm, DI water, 1mL/min, 2s loop and mounting bracket, CE 4900
Drift Less than 20nS/cm/h, DI water, 1mL/min, PowerStream software and system
Cell Volume 0.5µL controller, 4150 05 00 installation kit,
Cell Body PEEK P1000 grade interconnecting leads, power cable
Electrodes 316 stainless steel and operators manual.
Cell Pressure Rating 200psi
Cell Enclosure Heater 20 watt IonQuest System 2
Operating Temperature 25 – 50ºC; 35ºC standard As System 1 plus CE 4715 Suppressor system
Temperature Precision ± 0.01ºC For Anion background Suppression.
Warm-up Time 10 minutes typical
IonQuest System 3
LCD Screen 4 lines, back lit, adjustable contrast
As System 2 plus CE 4800-100
Method Storage Up to 100 with password protection
AutoQuest autosampler
Analogue Output 0-1V
Computer Interface RS232 Optional Items
Remote Autozero By contact closure
4720 01 01 Overpressure valve to
Digital Control By PowerStream software protect suppressor cartridge
Keypad Control For entry of values, commands and
programmes CE 4020 Solvent degasser 2 channel
Pump CE 4100 CE 4800 AutoQuest autosampler with valve
Pump Type Dual piston design
Analytical Columns
Flow Rate 0.001 to 10mL/min in 0.001 steps
Flow Reproducibility Better than 0.1% 4100 60 06 Strong Anions 7µm
Maximum Pressure 40MPa – 400 bar 4100 60 09 Strong Cations 7µm
User Interface 4 line back-lit display and full range of keys
4100 60 10 Weak Anions 3µm
Pressure Cut-outs Adjustable for over and low pressures
Pulsation Better than ±0.5% at 2mL/min 4100 60 11 Weak Cations 3µm
Flow Path Materials Ceramic, ruby, sapphire, PEEK Guard Columns
4100 59 06 Strong Anions 7µm
Column Oven
Number of Columns 2 plus suppressor cartridge 4100 59 09 Strong Cations 7µm
Pressure Cut-out Valve Accommodated – Option 4100 59 10 Weak Anions 3µm
Temperature range 5 ºC to 70 ºC (maximum 12 ºC below ambient)
4100 59 11 Weak Cations 3µm
Temperature Stability ±0.01ºC
Temperature Display 3 digit LCD display
Workstation Computer System
Injection Valve
Rheodyne Valve PEEK flowpath, 100mL loop
Solvent Degasser
Degasser – Optional In-line degasser
Number of Flow Paths Two, up to 3 mL/min each
Flow Path Material PTFE, PEEK ● PC with Pentium class processor
● Pentium 1 GHz or higher
System Details ● Microsoft Windows Vista, XP, 7, 8, 10
Power Requirements Total 110V or 240V, 50/60Hz, 200VA ● RAM 512 MB or higher
Stack Size 365 x 290 x 470mm (WDH) ● Hard disk space 150 MB
● CD-ROM drive
● XGA or higher resolution monitor
monitor resolution 1024 x 768 or
better
● Mouse or compatible pointing
device
● Free USB port, or RS232 serial port,
or serial to USB converter

CAMBRIDGE CB24 6AZ ENGLAND ISO 9001 : 2000
E-MAIL: info@cecilinstruments.com Email: www.cecilinstruments.com
8
DISSOLUTION
SYSTEMS
SOLUTION TESTER
ECTROPHOTOMETER CHOICE
UBLE BEAM
NGLE BEAM
LL ACCESSORY RANGE
DERING
ECIFICATIONS
CE 3790 Dissolution Tester CE 3792 Software - WIN DISS
Cecil Instruments offer complete dissolution systems This is a comprehensive dissolution software package
as well as all the components of the system for control and data processing, incorporating the
individually. The dissolution tester is common to the following features:
systems and may be used in conjunction with a choice of
spectrophotometers. It is fully USP, EP and JP compliant. Storage for 100 methods; stores stirrer speed,
temperature, sampling sequence, measuring
The CE 3790 tester is provided with seven stirred wavelength etc.
round bottom, graduated, glass vessels; the eighth
position is used for stirring the thermostatted water in A time run data store for batch number, name,
the perspex bath. The water is heated by a 1000 watt operator ID, calculation details, data correction using
element and the temperature may be set between 30˚C run or pre-run blank, media evaporation etc.
and 45˚C with an accuracy of ±0.2˚C. Calculation details include theoretical maximum
absorbance, measured standard concentration,
* The CE3890 is available with 8 stirred vessels. standard measurements using linear regression and
standard concentration at known testing times.
Stirring speed may be set in the range 20-250rpm.
The stirring head is conveniently pneumatically Results are printed in tabular and graphical form
counterbalanced for easy access to the vessels and including absorbance data, milligrams or % release
paddles for adjustments. The standard paddles are of and a 95% confidence calculation is provided. The
PTFE with PTFE coated spindles. Provision is made printout may be formatted by the operator.
for accurate centring of vessels and paddles, using
test gauges, and gauges are provided for stirrer The statistical programme includes batch comparison
height adjustment. and Q1-Q3 USP acceptance test.
2
This symmetrical double beam spectrophotometer
from the Aquarius series, with an 8 cell changer, has a
1.8nm optical bandwidth and is ideal for dissolution
measurements. Its stability of, ±1 x 10-4A/hour,
approximately ten times better than single beam
instruments, makes it ideal for extended
measurements such as sustained release monitoring.
Full cell and wavelength programming together with
wavelength scanning make the instrument ideal for
dissolution applications and may, when required, be
used as a high quality scanning double beam
spectrophotometer for other applications.
Operation is simple following menus and prompts

displayed on the LCD screen, using the keypad to
interact with the comprehensive embedded software.
Validation software is available. Used in System 1.
This is a top performance double beam spectro-
photometer from the Super Aquarius Series. The
optical system is fully symmetrical and uses a high
performance end window photomultiplier as detector.
This ensures the very best performance with minimum
drift and the best possible noise performance,
particularly for low energy conditions.
Both method and spectral scan storage are provided

together with powerful software for all applications.
For top performance this is the instrument of choice.

Validation software is available. Used in System 3.
CE 3021D Spectrophotometer
This is a high quality single beam spectrophotometer
from the Super Aurius series, with an 8 cell changer
and 1.8nm optical bandwidth. Its stability of 1 x 10-3
A/hour makes it suitable for dissolution monitoring,
and may be used as a general purpose or scanning
spectrophotometer if required.
Full wavelength and cell programming, as well as

wavelength scanning, are possible. Operation is via
embedded software using an LCD screen to display
menus, prompts etc. with entries made through its
comprehensive keypad. Enhanced accuracy is
provided, as with the CE 7260, by the very low, 0.01%,
straylight. Software for validation is available. Used in
System 2.
CE 3791 Peristaltic Pump

The CE 3791 is a multi-cartridge design with good flow
accuracy. Each of the eight channels may be individually
released, or adjusted for roller operating pressure.
The pictures shown here are of the pump with flow

tubes and the pump with one of the cartridges in the
released position.
3
CTROPHOTOMETER CHOICE
UBLE BEAM
Double Beam System
GLE BEAM
Shown here is the dissolution
tester used with the CE 7260
double beam spectrophotometer;
L ACCESSORY RANGE this forms the basis from which
dissolution System 1 is formed.
The compact tester and

DERING spectrophotometer occupy only
a small amount of bench space.
The tester has 7 stirred sample

CIFICATIONS vessels, the eighth position is
used for circulation of the water,
heated by a 1000W element. The
system and data collection are
controlled by the CE 3792 WIN
DISS software which can be
installed in most available PC’s.
Sample Compartment
The sample compartment of the

CE 7260 spectrophotometer is
shown with the eight cell
automatic changer fitted. As
shown, a reference cell may be
accommodated in the reference
beam.
The instrument is fitted with a

light-tight port through which all
the sampling tubes pass for
connection to the flowcells.
Automatic Cell Changer
The cell changer is shown here

removed from the instrument
and fitted with its eight cell
holder.
Fitting the changer to the

instrument is simple and rapid,
as is fitting the flow tubes to the
flow tube port. The same
changer is used with the CE 9260.
4
Single Beam System
This picture shows the CE 3790

dissolution tester coupled to a
CE 3021D single beam
spectrophotometer.
The small size of the

spectrophotometer coupled with
the compact design of the tester
lead to a system requiring only
the minimum of bench space.
This combination is the basis

from which System 2 is formed.
The system uses the same

CE 3792 WIN DISS software used
by the double beam system 1,
collecting data and providing
system control. Also the tester
provides 7 stirred vessels with
the eighth position used for
circulating the temperature
controlled water, heated by a
1000W element.
Sample Compartment
The eight cell auto-changer is

shown here in position in the
single beam spectrophotometer.
The compact compartment
accommodates the holder fitted
with its eight cell changer in a
convenient fashion, with all the
sampling tubes passing through
the special flow tube port.
Automatic Cell Changer
The compact and convenient to

use eight cell changer is shown
here, fitted with the eight cell
holder, before fitting to the
instrument.
The flow tubes are tidily guided

to the flowcells from the flow
tube port fitted to the instrument.
5
E BEAM
CCESSORY RANGE
ING
ICATIONS
Dissolution Tools Dissolution Baskets

A full range of tools is available for USP methods 1, 2 Dissolution baskets are available in a range of mesh
and 6. These include baskets in stainless steel, or gold sizes to meet most requirements. Mesh sizes of 10, 40
plated, and paddles in stainless steel, or Telfon coated. and 100 mesh grades are available in standard form or
Rotating cylinders are available, and devices for gold plated.
Transdermal studies of gels, suspensions and ointments.
Capsule Sinkers Depth Gauges

These devices, ideal for formulations which will not These devices allow rapid and accurate setting of the
sink under gravity, are made from stainless steel with stirring tool depth, and also vessel centring with a
snap tight closures and conform to Japanese centring option. The devices simply snap onto paddle
Pharmacopoeia Specifications. or basket tools.
Wobble Tester Mesh Discs

This tester, consisting of a spindle mounted dial gauge, For paddle over disc methods, (USP method 5), two
enables any rotational wobble of the dissolution tool in sizes of mesh disc are available, 35 and 85mm
its vessel to be measured and adjustments to be made diameter, which fit directly into standard dissolution
to give less than ±0.2mm wobble. vessels.
Dissolution Vessels Manual Sampling

Graduated dissolution vessels, in clear glass, are A syringe system is available for simple and
available in standard or certified form. Vessels are effective manual sampling from dissolution vessels
numbered and specifications are to the USP standard. and can be supplied with either a 5, or 10 micron
UV resistant amber glass vessels are also available. filter.
6
CE 3790 Tablet Dissolution Tester ORDERING SYSTEMS
Supplied complete; includes 7 graduated round
bottom vessels, 7 plastic covers for vessels, perspex For convenience of ordering, Pharma Quest systems
water bath mounted on a stainless steel base, are offered which contain all the items required to
polycarbonate bath cover, water heating coil, water assemble a working system and commence operation
circulation stirrer, drive head with pneumatically when flowcells and a PC with the appropriate
assisted lifting system. 7 PTFE paddles with coated specification are added.
shafts, connecting cable to PC, stainless steel sample
tubing set, 2 sets silicon tube for peristaltic pump, set System 1: With Double Beam Spectrophotometer.
of tygon tubing for connecting to pump and from CE 3790, CE 7260, CE 3791, CE 3792 and all
pump to spectrophotometer, immersion depth gauges. items to complete the system. Requires
flowcells and a PC.
CE 9260 Double Beam Spectrophotometer
190-800nm wavelength range, 1.8nm optical System 2: With Single Beam Spectrophotometer.
bandpass, dissolution sample compartment with flow CE 3790, CE 3021D, CE 3791, CE 3792 and
tube port, 8 position cell changer, 8 cell holder, power all items to complete the system. Requires
cable, cable to PC and operators manual. flowcells and a PC.
CE 7260 Double Beam Spectrophotometer System 3: With Double Beam Spectrophotometer.
190-1100nm wavelength range, 1.8nm optical As System 1, with CE 9260 Super Aquarius
bandpass, dissolution sample compartment with flow spectrophotometer instead of the CE 7260
tube port, 8 position automatic cell changer, 8 cell Aquarius spectrophotometer.
holder, power cable, cable to PC and operators manual.
CE 3021D Single Beam Spectrophotometer Alternative Dissolutions Systems

Systems may be based on the top of the range Super
190-1100nm wavelength range, 1.8nm optical
Aquarius double beam spectrophotometers CE 9260
bandwidth, dissolution sample compartment and lid,
and CE 9560. The CE 9560 is a variable resolution
flow tube port, 8 position cell changer and cell holder,
instrument with band width selectable from 0.5nm to
power cable, cable to PC and operators manual.
4nm and the CE 9260 has a fixed bandwidth of 1.8nm.
CE 3791 Peristaltic Pump Both instruments have a photomultiplier detector and
superb overall performance.
Eight channel variable speed peristaltic pump.
Both of these instruments use the same automatic 8
CE 3792 Dissolution Software cell changer and holder that is used in the Aquarius
For dissolution system control and data processing. CE 7260.
ORDERING ACCESSORIES AND SPARES

Flowcells - UV Grade Silica Depth gauge for paddle stirrers 3790 02 03
1mm pathlength 202 07 80 Capsule sinker set, stainless steel 3790 02 04
2mm pathlength 202 07 81 Spindle with basket set; 10 grade mesh stainless steel 3790 03 01
Flowcells - Optical Glass Mesh disk set, 35mm diameter (USP/5) 3790 04 01
1mm pathlength 303 07 80 Mesh disk set, 85mm diameter (USP/5) 3790 04 02
2nm pathlength 303 07 81 Manual sampling syringe system 3790 04 03
5mm pathlength 303 07 82 Plastic cover for vessels 3790 04 04
10mm pathlength 303 07 83 Cover insert for water circulator position 8 3790 04 05
Spectrophotometer Spares Stirrer shaft plastic adjustment screw 3790 04 06
Deuterium lamp 2202 01 42 Tubing and Filters
Tungsten halide lamp (in pairs) 2303 01 40 Silicone tube kit for pump 3790 05 01
Fuse kit for CE7260 9070 27 00 Tygon tube installation kit 3790 05 02
Fuse kit for CE3021D 2020 27 00 Sintered filters - 10 micron (50 pcs) 3790 05 03
Vessels and Stirrers Stainless steel sampling tubes and connectors set 3790 05 04
Graduated 1L clear glass vessel; certified 3790 01 01 Calibration
Graduated 1L brown glass vessel; certified 3790 01 02 Stirrer wobble gauge, mounted 3790 06 01
PTFE paddles and coated stirrers, batch coded, set 3790 02 01 Opto-electronic tachometer 3790 06 02
Stainless steel paddles and stirrers, batch coded, set 3790 02 02 Digital thermometer and sensor; calibrated 3790 06 03
7
CE 7260 Aquarius and CE 7460 CE 3021D Super Aurius
Optical System Symmetrical double beam Single beam

Monochromator Czerny Turner; 1200 line/mm grating Littrow, 1200 line/mm grating
Wavelength Range CE 7260 190-900nm 190-1100nm
CE 7460 190-1100nm
Wavelength Accuracy 0.5nm 1nm

Optical Bandwidth 1.8nm;
1nm option for CE7460
Straylight Typically less than 0.01% at 220nm and 340nm
Stability Better than 0.0001A/hour Better than 0.001A/hour
Photometric Range - 0.3 to 3A
Photometric Accuracy ±0.004A at 1A The greater of ±0.005A or 1%
Cell Program For up to 8 cells

Method Storage For up to 30 methods, password protected
Wavelength Program For up to 10 wavelengths
Wavelength Scanning Up to 4000nm/min
Computer/Printer Interfaces Bi-directional serial RS232 and parallel ports
Power Requirements 110V-250V, 50/60Hz, 170W
Size and Weight 635 x 410 x 210mm, 35kg 480 x 340 x 205mm, 18kg
The CE 9260 Super Aquarius spectrophotometer is a top performance spectrophotometer equalling or exceeding the
specification of the CE 7260 in all respects.
Dissolution Tester Specification Minimum Computer Specification

Number of Stirred Vessels 7 - CE 3790; 8 - CE 3890
Stirrer Tools Paddles, baskets and ■ Pentium 200MHz or better
cylinders ■ Microsoft Windows 98 or better
Water Bath Heater 1000W; with stirrer
Stirrer Speeds 20-250rpm ■ Microsoft NT 4.0 with service pack 3 or later
Stirrer Speed Accuracy ± 1 rpm
■ 64 MB of RAM or more
Stirrer Wobble Less than ± 0.2mm
Bath Temperature 30-45˚C ■ Hard disk space required : 15MB
Temperature Accuracy ±0.2˚C
Interface RS232 ■ CD-ROM drive
Bath Temperature LED display ■ XGA or higher resolution monitor
Stirrer Speed LED display
Stirrer Head Opening Pneumatically supported ■ Monitor resolution 1024 x 768 or better
ISO 9001 : 2000
TELEPHONE: 01223 420821 FAX: 01223 420475
CERTIFIED COMPANY

Catalogo CECIL

Caricato da

Informazioni sul documento

Copyright

Formati disponibili

Condividi questo documento

Condividi o incorpora il documento

Opzioni di condivisione

Hai trovato utile questo documento?

Questo contenuto è inappropriato?

Copyright:

Formati disponibili

Catalogo CECIL

Caricato da

Copyright:

Formati disponibili

ADVANCED - LOW COST - UV / VIS

High Quality Low Cost

The new lightweight designs offer an ideal

Convenient Operation Deuterium Lamp Save

SIP Flowcell Sampling SAMPLING

Performance with Convenience Ease of Operation

Concentration Curve Fitting

Scans at up to 4000nm/min are displayed with

The Super Aurius range offers in addition:

Cell and Wavelength Programming

Wavelength Scan Assay Program

Derivative Spectra Concentration Measurement

Overlayed Spectra Curve and Line Fitting

Dynamic Scan Recall Reaction Kinetics

Band Area Height Computation DNA and Protein Assays

Scan Storage Ratio and Difference

Programmed Scans Cell Program

Time Course Plots Wavelength Program

SYSTEM FUNCTIONS SAMPLING

DataStream - Fast Data Transfer and Control Cells and Flowcells

Self Test and Calibration Automatic Cell Changers

Method Operation and Storage Sipette Sampling

Software Enhancement by E-SEF Micro Sampling

Data Storage with Security Ultra-Micro Sampling

Display Scrolling for Wide Viewing Sample Temperature Control

Reprocessing Temperature Programming

Multi-Language Operation Batch Sampling

Performance Validation Dissolution Testing

Fast Program Any Assay

Any quantitative assay may be rapidly input for automatic

Transcription and calculation errors are avoided and validation

Assays may use up to 10 wavelengths and 8

Tabulation of results is automatic and the

Multi-Wavelength Assays Flexibility

Spectral information is often masked by overlapping

Derivatives may be displayed alone or superimposed

For accurate quantitative work it is often required to

A very easy to use programme is provided for this

Band quantification may be stored as a method

Regression analysis is applied to the user selected

Water and thermoelectric sample temperature control

Both Aurius and Super Aurius

Complete systems are offered, as

The Super Aurius should be used

Performance Validation Optical Bandwidth

Validation Using Filters

Absorbance and Wavelength validation are most

Straylight may be measured using the Pharmacopoeia

The ASTM tests at 220nm and 340nm are more widely

Micro Sipette Sampling SOFTWARE MODULES

For rapid measurement of samples Assay Program

Automatic Cell Changers Water Thermostatting Sampling Accessories

Cell Holders Part No. Temperature Control - Water Part No.

UV Lamp One Year Guarantee

CECIL INSTRUMENTS LIMITED MILTON TECHNICAL CENTRE

Automatic Wavelength Selection

Assays Displayed on Screen

A plot of any curve or straight line fitted to a

The printout on the left of this page shows a

OPTIONAL SOFTWARE - E-SEF Enabled ORDERING

Kalb and Bernlohr SPECIFICATION

SM5 Validation: Wavelength, Absorbance, Bandwidth etc

Bio Spectrophotometers Reaction Kinetics

Optical System Single Beam Symmetrical Double Beam BioQuestTM