MUSHROOM FARMING

1.) Preparation for “substrate”

2.) Composting (21 days)
3.) Fruiting bags
4.) Pasteurizing of fruiting bags (7 hrs.)
(15 psi)
5.) Inoculation
6.) Incubation (45 days)
7.) Fruiting
MATERIALS
 6” x 12” x .03 Polypropylene(pp bags)
 Cotton plugs or cotton waste
 ¾” PVC neck
 Rubber band
 Alcohol lamp
 Steel drum
 Mushroom house
 Sprayer
 Weighing scale
 LPG
PROCESS TO MAKE MOTHER CULTURE
Materials:

 Potato (250g)  Alcohol lamp

 Distilled water (1 lit.)  Inoculation needle
 White or brown Sugar (3 tbsp.)  Blade
 White Gulaman bars (3 bars)  Cotton
 Good & fresh mushroom for culture  Aluminum foil
 Cracked Corn  Flat bottle
 4” x 11” x .03 PP bag  Alcohol

PROCEDURE FOR CULTURE MEDIA

 Boil the 250gpotato in the 1 lit. Distilled water and remove the potato after boiling.
 Add 3 tbsp. sugar and the 3 barsgulaman bars while boiling mix well.
 After boiling about 5 min. transfer the cooked PDA to the flat bottle with about 40ml.
 Sealed the bottle with cotton plug & aluminum foil and sterilize it in pressure cooker with about 20
min. starting from its boiling pressure.
 Let the flat bottle place sided and cool off.
 After coolingdown,wipe the mushroom with alcohol and slice a small piece tissue in the center from
the young mushroom
 Put the tissue inside the bottle with PDA at the center
 Sealed the PDA in the bottle with clean cotton.
 After 2weeks mother culture is ready for transferring to grain spawn
 Boil the cracked corn for about at least 5 min., mix well while boiling.
 Drain and dry the cracked corn for about 60% moist.
 Packed the cracked corn with about 250g in the PP bag.
 Sterilize it in pressure cooker with about 20 min. starting from its boiling pressure.
 Transfer the culture media to the grain spawn with just a small amount by the process of heating the
bottle mouth before and after transferring.
 After 2-3 weeksGrain spawn is already ramified and ready for inoculation

Note:
 Must use clean chamber for processing
 Must be sanitized hands, no accessories and clean nails.
 Must be medium fire only for sterilizing.
 Don’t open the pressure cooker after sterilizing the bottle it might blow the bottle, cool down
first.
 Make sure the cotton plug must be extended from the mouth of the bottle for the holder.
 Remove water from the PDA after sterilizing and before transferring tissue.
 Don’t hold the cotton plug of mother culture when transferring the culture media to grain
spawn.
 Make sure when transferring culture media it’s not too much away from the fire
 1 Mother Culture bottle can produce 30 packed 250gGrain spawn
 1 lit. of Distilled water can produce 18 bottle of Mother Culture
Ang Oyster ayisanguringkabutenamgapinapapatubosamga fruiting bags naang substrate ay kusot, dayami, dahonngsaging at iba pa.
Angmgasangkap, gamit at hakbangsapaghahandang fruiting bags nito ay angmgasumusunod:

Mgamateryales (Kusotna Substrate)

78 kg. tuyongkusot (good or coco lumber)

20 kg. rice bran o darak
1 kg. brown sugar
1 kg. lime stone mulasamgakabebe (sea shell)
200 pcs. 6” x 12” x .03 polypropylene (pp) bags
200 pcs .cotton plugs
200 pcs. Pvc neck ¾” diameter x 5mm length
200 pcs. Ordinary rubber band
Steamer 200 liter steel dram

Paghaluinangmgasangkapnakusot, darak, lime at brown sugar. Haluingmabutiangmgasangkap.

Basainangmgasangkapnaumaabotsa 60 % moisture content (mc) angkailangansaisang proportion na halo.
Maaringilagaysa fruiting bag. Kung hindi pa gagamitinmaarininyongisako at ilagaysalugarnahindinababasa. Siksikinang substrate

 Punuinnginimbaknakompostnakusotangpp bag, siksiking mabutisapamamagitanngpansiksiknabote. Naglalamanng 1kg.

bawat fruiting bag para sa GanodermaLucidum at 800 grams namansa Oyster Mushrooms.
 Lagyanng PVC pipe angbibigng plastic bag nasyangdaananng mycelium o daananngmagigingbunganito.
Takpanngbulakangbibigng bag.
 Takpanng plastic angbibigng fruit bag sapamamagitanngpagtalinggumasailalalimngpvc,
upanghindimatanggalangnakatakipna plastic sabibigng fruit bag.
 Maglagayngpatungan o strainer saloobng dram na may sukatna 6” angtaas, lagyanngtubigna may sukatna 5” anglalim.
Isalansanangmga fruiting bag saloobng dram, takpanng plastic at sakoangbibigng dram at lutuinang fruiting bag saloobng 5 hours.
 Pagkatapos ay palamiginangmga bag, gawinangpaglagayngbinhisaisangmalinis at sterilisadonglugar. (inoculation )

Inoculation o PagtatanimngBinhisa Fruiting Bags

Mgamateryales at kagamitan

Sterilized fruiting bags

Spawn ngpleurotus
Alcohol lamp
Lastiko o guma
Papel o dyaryo

Paraanngpagtatanim

 Sapaglalagayngbinhisa fruiting bags itapatangbibigngboteng planting spawn saibabawngapoy. Initinsaapoyangspatsula at

palamiginbagoipasoksabibigngboteng spawn.
 Paghiwahiwalayinangmgabutona may amagsapamamagitanng transfer needle o spatsula. Magbukasng fruiting bag at
ibuhosangmgabinhisaibabawnito at punuinangbutasnito.
 Lagyanngpvc neck at takpankagadng cotton plug at lagyanngpapelangtinanimang fruiting bag
upangmaiwasanangkontaminasyon.
Incubation at pamumungasa Fruiting Bags

 Pananatilihinangmga fruiting bag saloobngmadilimnasilidsaloobng 45 days hanggangsakumalatangputing mycelium o amag

ay kumalat o bumabanghustosaloobng bag na may temperaturanaumaabotsa 28-32 degresscelcius.
 Hantayinang putting pustula (molds) nasiyangtandanaangmga fruiting bags ay handangmamunga o
magkaroonngtuboansyangtinatawagnakabute (mushroom).
 Sa ika-45 hanggang 48 naaraw ay maarinaitongipahiga o isalansansa cabinet hanggangito ay mamunga.
 Saika 50 daystanggalinangbulak at papel, kapag may lumusotngbungasapapelantayinmunanglumaki at anihin,
bagotanggalinangbulak at papel.
 Diliganito 3 times a day lalonasa tag-init at 2 besesnamansapanahonng tag-lamiggamitanghandspray o atomizer.
 Kapagmakalimangani (flushing) maarinangbuksanang fruiting bag sapamamagitanngpag-alisnangpvc, at
hayaangnakabukasang fruiting bags.
 Simulapagbukasng fruiting bags ito ay mag-produce ngkabutesaloobngisangaraw at ito ay lalaki at pedenangpitasinsaloobng
3 araw.
 Pagkatapospitasin, maghintayngtatlohangganglimangaraw at sisibolngmuliangkabutenatinatawagna (flushing). Sa pag-
aanihuwagputulinngkutsilyo o anumangmatalasnabagay, kailanganwalangmaiwanna stalk o sangasaloobng fruiting bag sanhi
itosapagkamatayngsusunodnabunga, dahilansapagkabulokngmgaito at simulanngkontaminasyon.
Angtamangpamamaraansapagpitas, hawakanangtangkayngkabute at marahanitongbunutin.
 Pagkatapospitasindiligankaagadang fruiting bag, kayangumaning 350 to 500 grams bawatisang fruiting bag na may lamang
1 to 1.5 kilo ng substrate saloobng 3 nabuwansimulangito ay sumibol.

MUSHROOM CHICHARON
Ingredients:

Oyster mushroom (you can have as much as you want)

1 cup cornstarch
2 tbsp salt
2 tbsp powdered garlic
Dash of ground pepper