MultiGene Thermal Cycler

TC9600-G/ TC9600/TC6000-G/TC6000

Service Manual
Ver.1.0
PCR Disclaimer:
MultiGene Thermal Cyclers can be used for a variety of molecular biology applications
and techniques, including the Polymerase Chain Reaction (PCR). PCR and other
molecular biology processes and techniques may be protected by patents. Purchase of
this product does not convey a license or right to perform patented PCR processes.

Applied Biosystems holds some of the basic PCR patents that may or may not be
currently valid. For additional information on PCR patents held by Applied Biosystems
and information about acquiring any required licensing for using PCR techniques, please
contact Applied Biosystems at 800-327-3002 or 650-638-5800 or their website:
www.appliedbiosystems.com .
 TABLE OF CONTENTS
1 SAFETY PRECAUTIONS ..................................................................1
2 SYSTEM DESCRIPTION ..................................................................2
2.1 DESIGN CONCEPT ...................................................................................... 2
2.2 ELECTRIC SPECIFICATIONS ....................................................................... 4
2.3 PCBS DESCRIPTION ................................................................................... 5
2.4 WIRE CONNECTION DIAGRAM .................................................................. 6
2.5 PCBS’ CONNECTOR LOCATIONS ............................................................... 7
3 SERVICE MODE...............................................................................10
3.1 DIAGNOSTIC ............................................................................................. 10
3.2 LID CALIBRATION .................................................................................... 10
3.3 BLOCK CALIBRATION .............................................................................. 13
4 SOFTWARE UPGRADE ..................................................................17
4.1 INSTALL THE SOFTWARE.......................................................................... 17
4.2 INSTALL THE HARDWARE ........................................................................ 17
4.3 REFLASH THE NEW SOFTWARE ............................................................... 18
5 TROUBLESHOOTING ....................................................................20
5.1 ERROR MESSAGES ................................................................................... 21
5.2 TROUBLESHOOTING FLOWS .................................................................... 22
5.2.1 Beeper has no sound ..................................................................................22
5.2.2 LCM has no display ...................................................................................22
5.2.3 Buttons are not activated ...........................................................................24
5.2.4 TE Module and PT-1000 Checkup .............................................................25

6 DISASSEMBLY/ASSEMBLY...........................................................26
6.1 FRONT PANEL DISASSEMBLY ................................................................... 26
6.2 SAMPLE BLOCK DISASSEMBLY ............................................................... 27
6.3 TOP CASE DISASSEMBLY ......................................................................... 28
6.4 LID MODULE DISASSEMBLY .................................................................... 30
6.5 LID KNOB DISASSEMBLY ......................................................................... 32
6.6 MCU CIRCUIT BOARD DISASSEMBLY .................................................... 34
6.7 POWER CIRCUIT BOARD DISASSEMBLY ................................................. 35
6.8 SYSTEM FAN DISASSEMBLY ..................................................................... 38
7 SPARE PARTS LIST AND EXPLODED DIAGRAM ...................38
7.1 FRONT PANEL ASSEMBLY ........................................................................ 39
7.2 LID ASSEMBLY ......................................................................................... 40
7.3 TOP CASE ASSEMBLY............................................................................... 42
7.4 BOTTOM CASE ASSEMBLY ...................................................................... 44
7.5 FAN ASSEMBLY......................................................................................... 46
7.6 COMPLETE ASSEMBLY ............................................................................ 47
MultiGene Service Manual

1 Safety Precautions
Before servicing the MultiGene thermal cycler for the first time, please read this entire
service manual carefully. The information contained herein is provided to give an
understanding of the workings of the MultiGene. All service work must be carried out by
authorized service personnel. Labnet will not be responsible for injuries and or damages
caused as a result of unqualified or unauthorized persons performing service. All
necessary safeguards below must be taken when working on equipment operating from
the mains power supply.
Disconnect power supply before opening the machine or performing service on the
machine.
Only use original accessories and spare parts provided by Labnet.
To avoid burns, service the machine after the heating lid and block cool down.
Work at an earthed bench with wrist straps and earthed flooring.
This manual is not subject to the update product revision. If you find the repair
unit’s designs or components are different from those described in this Service
Manual, please ask Labnet’s Service Department for a current Service Manual
version. Please remember to record the serial no. and software version before calling
or e-mail Labnet’s Service Department for assistance.

High Temperature Label: Please be aware of the heated block.

Warning Label: Please be aware of the danger of electric shock or other dangers.

If you need additional assistance while using this service manual, please contact Labnet’s
Service Department for assistance.

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2 System Description
The MultiGene Gradient thermal cycler is a powerful, reliable and affordable thermal
cycler. It is equipped with a gradient function that enables the user to set a maximum
24 °C temperature gradient between the left and right rows in the sample block at each
temperature.
For product specifications, feature and operation procedures, please refer to the
“MultiGene Thermal Cycler Operation Manual” for more information.

2.1 Design Concept

The MultiGene thermal cycler is a Peltier-based device. Peltier(Thermoelectric Module
or TE) is used for heating and cooling of the sample block. A powerful
Microprocessor(MCU) is used to accurately control the system to reach a desired
temperature rapidly and stay for a set period of time. Users can use this unit’s
user-interface to program and store their methods for future usages.

The key components of the Sample Block are shown in Figure 1. The key components’
functions are described below.
Lid Heater Module : is used to create high temperature (60~65 °C or 100 ~ 115 °C )
on the top of plastic tube to prevent the condensation of water.
Tube : Sample storage
Block Module: tube holder and temperature conduction component. The block
module is Aluminum.
Thermoelectric Module (Peltier) : heating and cooling component
Heatsink : Heat dissipation component
Fan : to increase airflow circulation and heat dissipation

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MultiGene Service Manual

Lid Heater

Tube

Block Module

Thermoelectric
Module

Heatsink

Fan

Figure 1. Design of Sample Block

The design block diagram of MultiGene is shown in Figure 2. The temperature control of
Lid Heater Module uses PWM voltage control. The lid temperature is fed back to MCU
by a temperature sensor: NTC(Negative Temperature Coefficient ), while the Block
Module uses three more accurate temperature sensors: PT-1000Ω.
Both the Lid Heater Module and 96-well Sample Block have a Thermal Cutoff Fuse
which is used to protect the unit from overheating. When the MCU is abnormal or
electronic components are damaged, the Lid Heater Module and Thermoelectric Module
can’t be controlled and continue to heat up. To prevent the unit from damage, the Thermal
Cutoff Fuse will fail when the temperature exceeds specification.

Other key components of the System and their functions are described below.
Fan: is used to speed up the heat dissipation of Block module. The speed of fan is
controlled by MCU by a Constant Voltage method.
Key Pad: is used to key in the desired temperature, time and other settings.
Liquid Crystal Module (LCM): is used to display the protocols and settings.
Real Time Clock (RTC): is used to generate date and time info.
EEPROM: is used to store the protocols and settings.
Power Supply: is used to provide the unit the required power. Input voltage: AC100
~ 230V. Output voltage: DC +24V. Max. Output power: 500VA.

Note: Please use 7A fuse in power source 100~120/50~60Hz countries, while 3.15A fuse

3
is for power source 200~220V/50~60Hz countries.

Figure 2. Design block diagram

2.2 Electric Specifications

Parts Name Specifications

Thermoelectric 6 units, Max. Current: 6A, Max. Voltage: 17.5V

Module(TE)
PT-1000(Block 3 units, Platinum, 1000Ω at 0ºC
Temperature Sensor)
Thermal cut-off fuse One on Sample Block, and one on Lid Heater(Max. 157ºC)
Fan DC24V, 0.8A, 19.2W
LCM 240 x 120 pixels, backlight adjustable
Battery Lithium, 3V, 225mAh
Lid Heater Silicon rubber, DC24V, Max. Current: 2.5A
NTC(Lid Heater Sensor) Range: -40~250ºC; 100KΩ at 25ºC
Power Supply Switching; Input: AC85V-260V, Output: DC24V, 20A;
500W

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MultiGene Service Manual

2.3 PCBs Description

Parts Name Part No. Function
Power Board PCB TC96005-RP Provide DC voltages to Peltier, Fan, Lid
Heater and other components.
MCU Board PCB TC96006-RP Control heating and cooling of Sample
Block and Lid Heater Module and Others.
RS-232 PCB 193-pcr150-00 RS-232 port, USB port and LCD
brightness control.

5
2.4 Wire Connection Diagram
The wire connection diagram is shown below. Users can use this diagram to understand
the connection between the wires and the circuit boards.

RS232 Board LCM module Keypad module

(P/N:193-pcr150-00) (P/N:156-07p010-c1) (P/N: 215-000001-00)
CN501

150-p01c10-05 56-07p010-c1 215-000001-00

CN102 CN103 CN105 CN101

MCU Board (P/N:TC96006-RP)

CN107 CN108 CN106 CN104

150-p01c10-07 150-p01c10-06 150-p01c10-04 MSP430 FET

CN301 CN302 CN309

Power Board (P/N:TC96005-RP)

CN308 CN310 CN306 CN303 CN305

CN304
CN307

150-p01c10-03 150-p01c10-02 275-heater-00 123-12412h-f1

Power Supply Slide cover assembly Fan module Block module

(P/N:TC96007-RP) (P/N:TC96012-RP) (P/N:TC96004-RP) (P/N:TC96008-RP)

150-p01c10-01

Power Switch
(P/N:122-42608r-00)
Figure 3. Wire connection diagram

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MultiGene Service Manual

2.5 PCBs’ Connector Locations

Power Board PCB
Connector Link Wire Application
part number
CN301 MCU Board CN107 150-p01c10-07 Controls Signal from MCU
Board.
CN302 MCU Board CN108 150-p01c10-06 Temperature control Signal to
MCU Board.
CN303 Slide cover assembly 275-heater-00 Temperature control Signal from
( Heater module ) Slide cover assy.
CN305 Fan module 123-12412h-f1 Supplies power to Fan module.
CN306 Slide cover assembly 275-heater-00 Supplies power to Heater
( Heater module ) module.
CN308 Power Supply 150-p01c10-03 Supplies power from Power
Supply.
CN309 MCU Board CN106 150-p01c10-04 Supplies power to MCU Board.
CN310 Power Supply 150-p01c10-02 Controls power supply ON/OFF
of Block module.

CN301 CN308

CN309 CN310 CN303

CN302 CN306 CN305
Figure 4. Connectors of Power Board

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MCU Board PCB
Connector Link Wire Application
part number
CN101 Front panel assembly 215-000001-00 Keypad control signal to MCU
( Keypad module ) Board.
CN102 RS232 CN501 150-p01c10-05 Control signal to RS232 Board.
LCM backlight adjustment.
CN103 Front panel assembly 156-07p010-c1 LCM control signal from MCU
( LCM module ) Board.
CN104 MSP430 FET ---------- For firmware update only.
CN105 Front panel assembly 156-07p010-c1 LCM backlight power from
( LCM module ) MCU Board.
CN106 Power Board 150-p01c10-04 Supplies power from Power
Board.
CN107 Power Board CN301 150-p01c10-07 Control Signal to Power Board.
CN108 Power Board CN302 150-p01c10-06 Temperature Control Signal from
Power Board.

CN102
CN107 CN106
CN104

CN108 Pin 1 Pin 1

CN103 CN105
CN101
Figure 5. Connectors of MCU Board PCB
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MultiGene Service Manual

RS232 Board PCB

Connector Link Wire Application
part number
CN501 MCU Board CN102 150-p01c10-05 Controls signal from MCU
Board.
LCM backlight adjustment.

CN501
Figure 6. Connectors of RS-232 Board PCB

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3 Service Mode
In Service Mode, users can perform: 1) Diagnostic function to check the heating rate and
cooling rate of Sample Block, 2) Calibration Lid: to calibrate the lid heater’s
temperature, 3) Calibration Block: to calibrate the 3 different Peltier module set
points(Right, Center and Left).
To enter Service Mode, please choose Service Mode under SYSTEM function option.
The password is “8888”.

3.1 Diagnostic
After entering Service Mode, choose the Diagnostic function to check out the heating rate
and cooling rate of sample block. Press F1:START to start the heating and cooling rate
check. The result will be shown as below screen.

SERVICE>DIAGNOSTICS 2007/01/05 10:30

1 Temp 1 Temp 1 Temp

Temp 20.0 94.0 4.0

Time 0:30 0:30 0:30
Heat rate:03.2℃/S , Cool rate:02.3℃/S

EXIT

F1 F2 F3 F4 F5

Figure 7. Heating/Cooling rate screen

If the heating rate is slower than 3°C /s or the cooling rate is slower than 2°C, calibration
may be necessary.

3.2 Lid Calibration

To calibrate the temperature of lid heater, a calibrated thermometer equivalent to Dual
Channel Thermometer ( Cat. No.: 23609-230 ) from VWR ( http://www.vwr.com ) or
TM-363 from TENMARS ( http://www.tenmars.com/front/bin/home.phtml ) and a K-type

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 MultiGene Service Manual
thermocouple temperature probe are required.
Pierce the flat cap of a 0.2ml plastic tube and insert the thermocouple temperature probe
as shown in figure below. Place the plastic tube with thermocouple temperature probe into
well D6 of the sample block. Place 4 empty tubes at each corner of the block and close
the lid
well D6

Figure 8. Lid temperature probe location

Select “Lid Calibration” under Service Mode, the screen will display as below

CALIBRATION>LID 2007/1/5 10:50

HT105 +0.3 060.0 Time: 02:20

HT60 +0.0 060.0 HT60
ROOM +0.0 024.0
Cal. Lid Timer
Calibration
Value
Measured
HOT! Value
HT60℃ HT105℃ + - Exit

F1 F2 F3 F4 F5
.
Figure 9. Lid Calibration screen

We use a two-point(105 °C and 60°C) curve method to calibrate the temperature curve of
lid heater.

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Another feature of this mode is the ability to calibrate the Room Temperature which is
shown as “ROOM” in the Figure 9 above. If the room temperature is too high( above
30°C ), the unit will show an error message “ROOM > 30 °C” and terminate the
protocol.
Please follow the procedures below to calibrate the lid temperatures and room
temperature.

A. Lid temperature calibration at 105℃

Press F2: HT105℃. The temperature of lid will go up to 105 °C.
When the timer shown in the right-hand side of Figure 9 reaches five
minutes ( Time: 05:00 ), record the readout of the Thermometer.
Using the formula below to calculate the Offset temperature value:
Offset Value = Thermometer Readout - Measured Value (by lid’s
temperature sensor)
Input the sum of factory-calibrated Calibration Value and Offset Value into
HT105°C’s Calibration Value column to calibrate the lid temperature. Press
F3 or F4 to change the Calibration Value to minus or plus. The calibration
procedure might need to be repeated several times to make the Offset Value
under 1°C.
Calibration is complete once the Offset Value is less than 1°C.

B. Lid temperature calibration at 60℃

Press F1: HT60℃. The temperature of lid will go up to 60℃.
When the timer reaches five minutes (Time:05:00), record the readout of
the Thermometer.
Using the formula below to calculate the Offset temperature value:
Offset Value = Thermometer Readout - Measured Value (by lid’s
temperature sensor)
Input the sum of factory-calibrated Calibration Value and Offset Value into
HT60°C’s Calibration Value column to calibrate the lid temperature. Press
F3 or F4 to change the Calibration Value to minus or plus. The calibration
procedure might need to be repeated several times to make the Offset Value
smaller than 1 °C.
Calibration is complete once the Offset Value is less than 1°C.

C. Adjusting ROOM temperature

Place the temperature probe of a thermometer in the front vent as shown in
Figure 10. Move cursor to Room’s Calibration Value column.

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MultiGene Service Manual
Using the formula below to calculate the Offset temperature value:
Offset value = Thermometer readout - Measured value (by temperature
sensor on MCU board)
Input the sum of factory-calibrated Calibration Value and Offset Value into
Room’s Calibration Value column to calibrate the Room temperature. Press
F3 or F4 to change the Calibration Value to minus or plus. The calibration
procedure might need to be repeated several times to make the Offset Value
smaller than 1 °C.
Calibration is complete once the Offset Value is less than 1°C.

Place the thermocouple

temperature probe in the
front vent as shown in the
right to measure the Room
Temperature. Secure the
probe with a piece of tape.

Figure 10. Probe location to detect Room temperature

3.3 Block Calibration

There are 3 sets of two Peltier modules( 40mm x 40mm) under the 96-well sample block.
These 3 sets Peltier modules sit side by side. They are labeled as Left, Center and Right
module set as shown in Figure 11. The Gradient temperature function is created by the
temperature differences between these three Peltier module sets.
To calibrate the sample block, a thermometer and a temperature probe, like Alpha
Technics (http://www.alphatechnicsonline.com ), 4500 thermometer and SP230 0.2ml
well probe, are needed. A multi-channel temperature thermometer system, such as Alpha
Technics, 5020 thermometer (16-channel), or Quanta Biotech
(http://www.quantabiotech.com ), TAS Thermal Cycler Validation Systems or
CYCLERtest (http://www.cyclertest.com/products.asp ), DRIFTCON, is good for
simultaneous temperature recording of different wells.

13
 Right
Peltier set
Left Peltier set

Center Peltier
set

Figure 11. 3 Peltier module sets location

Please follow the procedures below to calibrate the temperature of sample block.
1. Prepare a thermometer and place the temperature well probe in 3 wells (D1, D6,
D12), as shown in Figure 12.

Well probe:
Thermometer:
Alpha Technic,
Alpha Technics,
SP230
4500

Figure 12. Temperature calibration setup

2. Enter System and select Lid Temperature. Select 2 OFF in Lid Temperature function.
Press “Accept”. Press “Exit”, and close the lid.
3. Select “Calibration Block” to display “Calibration Block” screen as shown in Figure
13. Please note “Uniform”(Uniformity) is a factory default setting. No
adjustment is necessary!

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MultiGene Service Manual

CALIBRATION>BLOCK 2007/1/5 11:10

Left +0.4 (D1) 94.0 Time: 02:20

Center +0.1 (D6) 94.0 BLK94
Timer
Right +0.0 (D12) 94.0
Cal. Block
Uniform +0.0 (A1,A12,H1,H12,D6)
(MAX-MIN)/2-0.5 Measured
Calibration Value (D1)
Value
HOT!
BLK94 BLK40 + - Exit

F1 F2 F3 F4 F5

Figure 13 “Calibration Block” screen

A. Block temperature( Left, Center, Right ) calibration at 94℃

Press F1:BLK 94. The temperature of the sample block will go up to 94℃.
When the timer reaches five minutes (Time:05:00), record the
Thermometer Readout of well D1 to calibrate the left Peltier module set
(Left).
Using the formula below to calculate the Offset temperature value:
Offset Value = Thermometer Readout(D1) – Measured Value (D1)
Calculate the new calibration value by adding the factory calibration
value and the offset value.
Input the sum of factory-calibrated Calibration Value(Left) and Offset
Value into the Left Peltier module set’s Calibration Value column to
calibrate the Left Peltier module set’s temperature. Press F3 or F4 to
change the Calibration Value to minus or plus. It might be required to
perform the procedures above several times to make the Offset Value less
than +/-0.5 °C.
Temperature calibration of Left Sample Block is complete once the Offset
Value is less than +/-0.5°C.
Repeat the same procedures above for Center(Well D6) and Right(Well
D12) Peltier module set.

15
B. Block temperature( Left, Center, Right ) calibration at 40℃
Press F2:BLK 40. The temperature of sample block will go to 40℃.
When the timer reaches five minutes (Time:05:00), record the
Thermometer readout of well D1 to calibrate the left Peltier module
set(Left).
Using the formula below to calculate the Offset temperature value:
Offset Value = Thermometer Readout(D1) – Measured Value(D1)
Input the sum of factory-calibrated Calibration Value(Left) and Offset
Value into the Left Peltier module set’s Calibration Value column to
calibrate the Left Peltier module set’s temperature. Press F3 or F4 to
change the Calibration Value to minus or plus. It might be required to
perform the procedures above several times to make the Offset Value
smaller than +/-0.5 °C.
Temperature calibration of Left Sample Block is complete once the Offset
Value is less than +/-0.5°C.
Repeat the same procedures above for Center(Well D6) and Right(Well
D12) Peltier module set.

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MultiGene Service Manual

4 Software Upgrade
MultiGene’s software can be upgraded by using a USB software programmer: Texas
Instruments MSP-FET430 Flash Emulation Tool(FET) ( For more information, please
visit www.ti.com/msp430 ) and a PC or notebook computer.
The following items required for software upgrade include;
One Software Programmer: MSP-FET430UIF USB-to-JTAG Emulation Interface
“FET-Pro430-Setup” Windows Installer Package file. [ If you can’t find it in
MSP430 CD-ROM, please download and unzip “FET-Pro430-Lite-Setup.zip”
(P/N:CS014X01) file from http://www.elprotronic.com/download.html to retrieve
“FET-Pro430-Setup” file.]
One MSP-FET430UIF “READ ME FIRST” document
One USB cable, Type A/B
One 14-conductor cable
PC or notebook computer with Windows 2000 or XP

After the unit is turned on, the initial screen which contains software version will show up.
Follow the instructions below to refresh the new software version to the thermal cycler.
To ensure the performance of the product are within its specifications, the temperature of
block, lid and room must be recalibrated after the software refresh.

4.1 Install the Software

1. Run “FET-Pro430-Setup” Windows Installer Package file.
2. Click “Next” button on “FET-Pro430 v1.10 –Lite version” screen.
3. Choose “I Agree” and click “Next” button on “License Agreement” screen.
4. Fill in “Name” and “Organization” and click “Next” button on “Customer
Information” screen.
5. Choose “Everyone” or “Just me” and click “Next” button on “Select Installation
Folder” screen.
6. Click “Next” button on “Confirm Installation” screen.
7. Click “Next” button on “Very Important: Read carefully.” screen.
8. Click “Close” button on “Installation Complete” screen.

4.2 Install the Hardware

1. Plug in the MSP-FET430UIF software programmer to the USB Port of a personal computer

17
 (PC), using provided cable extender (USB-A to USB-B). Connect 14-conductor cable with
thermal cycler’s CN104 software upgrade connector and MSP-FET430UIF programmer as
shown in Figure 14.

MSP-FET430UIF
programmer

14-conductor cable:
USB cable:
Pin 1 is Red.
Connect to a PC.

Figure 14. MSP-FET430UIF software programmer setup

2. The “New hardware has been found” should be displayed, once the software programmer
has been connected to your PC.
3. If your computer can’t recognize MSP-FET430UIF as a USB device for any reason, point the
Hardware Installation Wizard to the appropriate folder where the corresponding driver
information files are located on your hard disc. Drivers in the previously installed software are
located(on a default installation) in directory:
C:\Program Files\Elprotronic\FET-Pro430 Flash Programmer\USB-Driver\WinXp
4. Driver installation process will start.
Note that Windows XP shows a warning that the driver is not certified by Microsoft.
Ignore this warning and click “Continue Anyway”.

4.3 Reflash the New Software

1. Run “Lite FET-Pro430 Elprotronic” program.
2. Choose “Setup” in menu. Select “Connection/Deice Reset” option.
Communication with the target devices can be selected in the “Target’s Connection/Reset
Options” dialogue screen..
3. Select “Standard JTAG” under “Communication with Target Device” column. Select
“Any” under “Reset Options”. Select “Switch Vcc Off” under “Final Target Device Action”.
Select “TI’s FET,USB-FET or hardware compatible-uses TI’s MSP430.dll library”
under “Used Adapter” column. Select “USB” under “COM port” column. Click “OK”
button when all the selections are done. The screen will move into the software reflashing
screen.

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MultiGene Service Manual
4. In software reflashing screen, type:MSP430-F: G4619 under “Microcontroller
Type” column.
5. Click “ Open Code File” button to select the new software version’s path. The new
software file(Format: *.txt) is provided by Labnet. Click “AUTO PROG.” button to
reflash the new software.
6. Once the installation is complete, the “Status” column will show “Pass”.
7. Turn On the unit to check the initial screen whether the software version has been
upgraded.

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5 Troubleshooting
Please refer the following problem and action list to diagnose and resolve the problems.
More troubleshooting flows are described in Section 5.2.

Problem Cause Action

The display is off even when 1. Power is not reaching the 1. Check power source
power is switched on. system. voltage.
2. Power cord is not 2. Reconnect the power
plugged into the socket cord.
properly. 3. Replace the power fuse.
3. Power fuse is blown out. 4. Return the unit for
4. Block fuse is blown out. service.
Can’t reach 4°C. 1. Operating temperature 1. Operate the unit in a
environment may be environment temperature
unsuitable. between 15 to 30 °C.
2. Electronic cooling 2. Return the unit for
element may be damaged service.
or aged.
Cycle time is too long 1. Operating environment 1. Operate the system in a
temperature may be temperature environment
unsuitable. between 15 to 30 °C.
2. Electronic cooling 2. Return the unit for
element may be damaged service.
or aged. 3. Return the unit for
3. Faulty temp. sensor. service.
Lid heater does not work. 1. Lid heater is set off. 1. Check Lid Temperature
2. Lid sensor problem setting in System Mode
under Calibration Lid.
2. Return the unit for
service.
No key entry sound. 1. Sound may currently be 1. Check Beeper setting in
set off. System Mode.
2. Faulty keys or keypad 2. Return the unit for
wiring. service.
The display goes off. 1. Faulty backlight. 1. Adjust the LCD contrast.
2. Faulty LCD panel 2. Return the unit for

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MultiGene Service Manual
service.
Display is too dark or light Backlight brightness is not Adjust Display Brightness
adjusted properly. Potentiometer.
Lid will not close. 1. Foreign matter, between 1. Remove the foreign
lid heater and sample object or matter.
block.
2. Faulty lid lock 2. Replace lid.
mechanism.
Error message appears. Refer to list of error Check the nature of the error
messages below. and take the suggested
actions.

5.1 Error Messages

Message Cause Action
HEATER ERROR! Lid temperature sensor problem. Reboot the unit.
Lid temperature exceeds 132 °C.
Lid temperature can’t reach the set
temperature.
BLOCK ERROR! Block temperature sensor or heating Cool down the room
element problems. temperature. Reboot the unit.
The unit can’t reach the set
temperature, because the room
temperature is too high.
The block temperature exceeds 108
°C.
ROOM ERROR! Room temperature sensor problem. Reboot the unit
ROOM > 30 °C The room temperature exceeds 30 Cool down the room
°C. temperature. Reboot the unit.
Two running units are too close to Remove any objects
each other. blocking the vents.
Keep two running units at
least 30cm from each other.

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5.2 Troubleshooting Flows
Please refer the flows below to analyze the defect problems.

5.2.1 Beeper has no sound

Users
turn off Yes
Turn ON Beeper
Beeper ?
in System Mode

No

Return to Labnet
for Repair

5.2.2 LCM has no display

There are a number items which may cause LCM display to fail. Please follow
the flow chart below to identify the possible problems.

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MultiGene Service Manual

LCM has no display LCM Backlight VR

Check No Adjust LCM

LCM backlight
NoVR?
backlight VR

Yes

AC power cable socket

Check Fuse socket
AC Power Cable No Insert AC cable to
Insertion ? socket tightly

Yes

Yes Change power

Is the power fuse
fuse LCM Panel cables
blown ?

No

Replace front
No
Are panel cables OK? panel and
cables

Yes

Block Fuse Digital Multimeter

Is Block Fuse Replace
Yes
burned ? Sample Block

No

Return to Labnet
for repair.

Block Fuse test :

Open (≒∞ ohm ): Broken; Short (≒0 ohm ) : OK

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5.2.3 Buttons are not activated
Front Panel Assembly
( P/N: TC96003-RP )
Buttons are not activated

Power On
the machine No
Turn OFF and
again?
ON the machine

Yes

Keypad Cable LCM Cable Back light Cable

Replace the
Front Panel No
Replace the
Assembly? Front Panel
Assembly
Yes

Return to Labnet
for Repair

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MultiGene Service Manual

5.2.4 TE Module and PT-1000 Checkup

a. Use a Digital Multimeter (BM859CF ) to check PT1000’s Ohm values.

At 25 °C, Values = 1095 ~ 1097 ohm OK
At 25 °C, Values < or > 1095 ~ 1097 ohm NG

a b c d e f g h i

a, b, c, g, h, I : 6 sets TE module wire pairs

d, e, f : 3 sets PT-1000 wire pairs

Digital Multimeter Programmable Impedance

:
(BRYMEN BM859CF) Meter ( ABM3365 )

b. Use a Programmable Impedance Meter ( ABM3365 ) check TE’s Ohm values .

At 25 °C, Values = 2.10 ~ 1.60 ohm OK
At 25°C, Values < or > 2.10 ~ 1.60 ohm NG

25
6 Disassembly/Assembly
Service engineers can follow the procedures below to disassemble the modules of the
thermal cycler step-by-step. Service engineers can reverse the procedures below to
assemble the modules. Please ensure the power cord has been unplugged during the
assembly/disassembly procedures.

6.1 Front Panel Disassembly

1. Use clip to remove two grey rubber pads as 2. Release and remove the two screws as
showed above. showed.

3. To open the front panel, use one hand to 4. Pull out the three cables as showed, and
push the front of front panel and the other then can disassemble the front panel.
hand to lift its rear.

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 MultiGene Service Manual

6.2 Sample Block Disassembly

Notch

1. Slide lid module to the middle position of 2. Use clip to remove the six rubber pads as
its track and open it through two notches. shown above.

3. Release and remove the two screws as 4. Release and remove the two screws as
shown above. shown above.

5. Release the four screws as shown above. 6. To remove the sample block module, hold
Please don’t remove them. the handle of the module and pull it out as
shown direction.

27
6.3 Top Case Disassembly

1. Release and remove the nine screws as 2. Release and remove the three screws as
shown above. shown above.

3. Pull out the two cables as shown above. 4. This is the photo after pulling the two
cables off.

5. Use left hand to hold the metal bracket as 6. When users open the top case, please note
shown above. Pull it toward inside direction one black power switch cable maybe jammed
when right hand lifts up the top case. by a metal parts as shown.

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 MultiGene Service Manual

RS-232 cable

7. Please release the jammed cable. DON’T 8. Open the top case and pull out the RS232
use force to pull the cable before release it. cable as shown above.

9. Top case disassembly is complete.

29
6.4 Lid Module Disassembly

1. Release and remove the screw as show 2. Insert the two connectors of heater module
above. cable one by one to an square hole on case
and pass though it to the outside of the case.

3. Pull these cables to the outside of top case 4. Use clip to take off the two rubber covers
carefully. as showed.

5. Push the lid module to the back end of its 6. Release and remove the inner two screws as
track. showed. You can’t do this step if the lid
module isn’t on the back end position.

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 MultiGene Service Manual

7. Lift the side of lid module as showed. 8. The lid module disassembled.

31
6.5 Lid Knob Disassembly

1. Use clip to remove the two rubber pads as 2. Release and remove two screws as shown
shown above. above. Two screws can’t be seen if the lid
module isn’t on the back end of its track.

3. Lift up the side of lid module as shown. 4. Turn the lid knob clockwise to lower down
and separate the heater module.

5. Turning the lid knob clockwise 6. Lid cover is disassembled.

continuously, the heater module will be
separated as shown above.

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 MultiGene Service Manual

7. Flip the lid cover as shown above. 8. Release and remove the two screws as
showed.

9. The lid knob is fully disassembled.

33
 6.6 MCU Circuit Board Disassembly

1. Pull out the three cables as shown above. 2. Release and remove the four screws as
shown above.

3. MCU circuit board disassembly is

complete.

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 MultiGene Service Manual

6.7 Power Circuit Board Disassembly

1. The three cables are managed as shown 2. Move the cables away from the original
above. positon as shown above.

3. Release and remove the five screws as 4. Don’t remove the aluminum foil.
shown above.

Fan cable

5. Open the power circuit board EMI 6. Pull out the system fan cable as shown
shielding. The power circuit board can be above.
seen.

35
7. Release and remove the two screws as 8. Release and remove the two screws as
shown above. showed.

9. Use both hands to hold the two sides of 10. Inner module is disassembled.
metal bracket and lift up whole inner module.

11. Pull out the two power terminal connector 12. Release the eleven screws and pull out
protection covers as shown above. these cables as shown above.

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 MultiGene Service Manual

Copper Stand

13. Use 5 m/m hex head driver to release and 14. Power circuit board disassembly is
remove the copper stand as shown above. complete.

37
 6.8 System Fan Disassembly

1. Release and remove the four screws as 2. System fan disassembled.

showed.

7 Spare Parts List and Exploded

Diagram
Please follow the spare parts list below to order your required spare parts. For easy repairs,
the spare parts are modularized. Only spare parts with check can be ordered.
Please refer the Exploded Diagram to service the machine.

38
 MultiGene Service Manual

7.1 Front Panel Assembly

PARTS LIST OF FRONT PANEL ASSEMBLY

Spare Parts Including
Item Part No. Description
Yes No Item
※ TC96003-RP Front panel assembly √ 1,2,3,4
49 207-000102-00 Front panel √ N/A
50 210-000002-00 LCD sponge (L) √ N/A
51 210-000001-00 LCD sponge (S) √ N/A
52 215-000001-00 Membrane keypad √ N/A
53 156-07p010-c1 LCD module WG240128B √ N/A
54 150-p01c10-08 Wire DC 24V L200 √ N/A
55 206-000025-00 LCD bracket √ N/A
(A) 202-130308-10 Screw PTS-4 3X8 √ N/A
(B) 201-530308-10 Screw SPW M3x8/w washer √ N/A

39
7.2 Lid Assembly

40
MultiGene Service Manual
PARTS LIST OF LID (SLIDE COVER) ASSEMBLY

Item Part No. Description Spare Parts Including Item

Yes No
30,31,32,33,
※ TC96012-RP Lid(Slide cover) assembly √ 34,35,36,(A),
(C),(G),(L),(M)
30 207-000106-00 Top knob √ N/A
31 206-000042-00 Knob pin √ N/A
32 206-000043-00 Knob latch √ N/A
33 205-074220-00 Spring 0.7x4.2x20 √ N/A
34 207-000103-00 Slide cover √ N/A
35 206-000034-00 Bearing 6905 LLU/2A √ N/A
36 206-000040-00 Bearing bracket √ N/A
37 206-000020-00 Heater press screw √ N/A
38 207-000112-00 Slide shaft (R) √ N/A
39 207-000111-00 Slide shaft (L) √ N/A
40 205-454515-00 Spring 0.45x4.5x15 √ N/A
41 206-000044-00 Knob pin fixer √ N/A
42 206-000030-00 Latch link √ N/A
43 206-000031-00 Latch cover √ N/A
√ 44,45,46,47,
※ T01-000081-00 Heater module assembly 48,49,50,51,
52,(B),(F),(G)
44 206-000021-00 Extend screw √ N/A
45 207-000110-00 Latch releaser √ N/A
46 205-022912-00 Spring 0.2x2.9x12 √ N/A
47 206-000032-00 Heater base bracket √ N/A
48 273-heater-00 Silicon rubber heater √ N/A
49 206-000033-00 Heater bracket √ N/A
50 205-684070-00 Spring 0.4x(6/8)x7 √ N/A
51 207-000107-00 Lid seal frame √ N/A
52 210-020002-00 Seal PORON √ N/A
(A) 202-130308-10 Screw PTS-4 3X8 √ N/A
(B) 201-530308-10 Screw SPW M3x8/w washer √ N/A
(C) 201-404208-10 Washer PW-4.2x10x0.8 √ N/A
(E) 202-130206-10 Screw PTS-4 2x6 √ N/A
(F) 206-000022-00 Heater bracket screw √ N/A
(G) 201-120305-11 Screw FMS M3X6 120D √ N/A
(H) 201-140306-10 Screw BMS M3*6 √ N/A

41
(L) 202-140304-40 Screw BTS-4 3x4 Black √ N/A
(M) 202-110204-40 Screw BITS 2x4 Black √ N/A

7.3 Top Case Assembly

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 MultiGene Service Manual

PARTS LIST OF TOP CASE ASSEMBLY

Spare Parts Including
Item Part No. Description
Yes No Item
19 207-000100-00 Top case √ N/A
20 207-000104-00 Slide guiding L √ N/A
21 207-000105-00 Slide guiding R √ N/A
22 207-000108-00 Spring cap √ N/A
23 205-056300-00 Spring 0.6X5X30 √ N/A
14 206-000026-00 I/O bracket √ N/A
25 193-pcr150-00 RS232_V03 PCB PCBA √ N/A
26 122-42608r-00 Power SW JS-608RA-B1B-2 √ N/A
27 122-12101q-00 Power socket JR-101-1FS(Q) √ N/A
28 150-p01c10-01 Wire AC L=480mm √ N/A
29 150-p01c10-05 Wire DC24V/0A5 L400 √ N/A
(A) 202-130308-10 Screw PTS-4 3X8 √ N/A
(B) 201-530308-10 Screw SPW M3x8/w washer √ N/A
(C) 201-404208-10 Washer PW-4.2X10X0.8 √ N/A
(D) 203-300507-10 Copper stand 4# 5X7 √ N/A

43
7.4 Bottom Case Assembly

44
 MultiGene Service Manual
PARTS LIST OF BOTTOM CASE ASSEMBLY
Spare Parts Including
Item Part No. Description
Yes No Item
1 207-000101-00 Bottom case √ N/A
2 208-000011-00 Rubber foot RW3005 √ N/A
3 TC96004-RP Fan module assembly √ N/A
4 206-000023-00 Main PCB bracket √ N/A
5 TC96006-RP MCU_V03 PCB PCBA √ N/A
6 206-000028-00 Power supply bracket √ N/A
7 TC96007-RP Power supply SP500-24 √ N/A
8 206-000024-00 System bracket √ N/A
9 206-000027-00 Heat-sink support bracket √ N/A
10 TC96005-RP POWER_V05 PCB PCBA √ N/A
11 201-300325-30 Stand off M3X25X6 √ N/A
12 206-000039-00 Power PCBA shielding √ N/A
13 150-p01c10-06 Wire DC 24V/0A5 L300 p=10 √ N/A
14 150-p01c10-07 Wire DC 24V/0A5 L300 p=12 √ N/A
15 150-p01c10-04 Wire DC 24V/0A5 L230 p=4 √ N/A
(A) 202-130308-10 Screw PTS-4 3X8 √ N/A
(B) 201-530308-10 Screw SPW M3x8/w washer √ N/A
(C) 201-404208-10 Washer PW-4.2X10X0.8 √ N/A

45
 7.5 Fan Assembly

PARTS LIST OF FAN ASSEMBLY

Spare Parts Including
Item Part No. Description
Yes No Item
1,2,3,(C),(I),(K
※ TC96004-RP Fan module assembly √
)
16 123-12412h-f1 Fan PSD2412PMB1-(2) √ N/A
17 208-000008-00 Fan bumper √ N/A
18 206-000038-00 Fan protect frame √ N/A
(C) 201-404208-10 Washer PW-4.2X10X0.8 √ N/A
(I) 201-130420-20 Screw PMS M4*20 √ N/A
(K) 201-200435-20 Nut M4x8 t=3.5 √ N/A

46
MultiGene Service Manual

7.6 Complete Assembly

47
PARTS LIST OF FINAL ASSEMBLY
Spare Parts Including
Item Part No. Description
Yes No Item
60 275-pcrln2-00 Bottom case assembly √ N/A
61 275-pcrln1-00 Top case assembly √ N/A
62 TC96012-RP Slide cover assembly √ N/A
63 TC96003-RP Front panel assembly √ N/A
64-1 TC96008-RP 96 wells block assembly √ N/A
64-2 TC96009-RP 60 wells block assembly √ N/A
65 208-000010-00 Screw rubber pad √ N/A
(J) 201-110310-10 Screw TMS H M3x10 √ N/A

48
 Labnet International Inc.
PO Box 841, Woodbridge, NJ 08837, USA
Tel: +732-417-0700 Fax: +732-417-1750
E-mail: labnet@labnetlink.com
Web Site: www.labnetlink.com

P/N: 402-PCR01-0708
Issue date: 2007/8