SIX MONTHS INDUSTRIAL TRAINING

REPORT
On
“TOXICITY STUDY OF THE GRAPHENE OXIDE NANOEMULSION ON
RAT”

Held at
INSTITUTE OF NUCLEAR MEDICINE & ALLIED SCIENCES, DRDO
TIMARPUR, NEW DELHI

Submitted in partial fulfillment of the requirement for the award of the

degree of
BACHELOR OF TECHNOLOGY
(“BIOTECHNOLOGY”)
Under the Supervision of

Internal Supervisor: External Supervisor:

“DR. MANJEET KAUR” “DR. HIMANSHU OJHA
“U.I.E.T” MDU SCIENTIST" D"
INMAS (DRDO)
SUBMITTED BY:
AADITI
Roll no: 13302

University Institute of Engineering & Technology

M.D.University, Rohtak-124001
 CANDIDATE'S DECLARATION CERTIFICATE

I hereby certify that the work which is being presented in the report entitled “TOXICITY STUDY
OF THE GRAPHENE OXIDE NANOEMULSION ON RAT” by “AADITI” in partial fulfillment
of requirements for the award of degree of B.Tech submitted to Department of (“Name of
Department”) of U.I.E.T.at M.D.U Rohtak is an authentic record of my own work carried out
during a period from 08/01/2018 to 08/06/2018 under the supervision of Dr. HIMANSHU
OJHA, SCIENTIST ‘D’

Signature of student

This is to certify that the above statement made by the candidate is correct to the best of my/our
know ledge

Signature of SUPERVISOR

Signature of HOD
 ACKNOWLEDGEMENT

I am prostrate to almighty god who is omnipotent, omnipresent and omniscient, and whose
benign benediction gave me the required zeal for the completion of this project work.

First of all, I am sincerely grateful to Dr. Mitra Basu, Scientist 'F' (HOD), CBRN Department
for his kind permission for work which I carried out in the institute.

I am thankful to my supervisor Dr. Himanshu Ojha, Scientist-'D', Department of CBRN

Defence, Institute of Nuclear Medicine and Allied Sciences, DRDO, New Delhi-110054 for
his invaluable guidance and encouragement in designing, implementation of experiments and
analysis of data .The dissertation wouldn’t have got completed without his insightful suggestion
and ungrudging support.

I pay my sincere and special thanks to Ms. Deepti Sharma (SRF) for his valuable suggestions,
constant and invaluable help, guidance and encouragement at every step to complete my
research work efficiently and successfully.

I would like to show my heartiest gratitude to my respected lab seniors Mr. Navneet Sharma
(SRF), Ms Shweta Kulshrestha (SRF) for their help and teaching me various laboratory
techniques.

I would like to thanks my lab mates Prerna, Shikha, Deepshikha, Prashant and Diksha for giving
me support during my project work.

And last but not least, I would like to thank my parents and beloved family members who have
given me the support whenever needed. They have always been a source of inspiration for me
and their prayer, affection and love made me reach this stage.

AADITI
CONTENTS:-

ACKNOWLEDGEMENT

LIST OF TABLES

LIST OF FIGURES

1. INTRODUCTION

1.1 Toxicity

1.2 Types of toxicity

1.3 Decontamination

1.4 Anatomy of the skin

2. REVIEW OF LITERATURE

2.1 Toxicity according to OECD guideline 410

2.2 Test condition

2.3 Different skin decontamination available or are under studies

2.4 Graphene

2.5 Graphene oxide

2.6 Nanoemulsion

3. AIM AND OBJECTIVES

4. MATERIALS AND METHODS

4.1 Materials required

4.2 Chemicals required

4.3 Instruments required

4.4 Description of Toxicity Test Procedure (Acc. to OECD)

4.5 Procedure

4.6 Histopathology
5. RESULT AND DISCUSSION

6. CONCLUSION

7. REFERENCES
 LIST OF TABLES

Tables

Table 1.Average weight of the Sprague Dawley rats

Table 2. Hematological parameters for blood of GO treated SD Rats

Table3. Biochemical parameters for blood of SD Rats treated with GO nanoemulsion.

 LIST OF FIGURES

Figures

Figure 1. Types of toxicity acc. to the duration and frequency of exposure

Figure 2. 3D structure of the skin

Figure 3. Epidermal and Skin layer

Figure 4. Structure of Graphene

Figure 5. Structure of Graphene oxide

Figure 6. Housing of the rats at “Animal house facility, INMAS, DRDO, Delhi”
(above) and an individual rat in a polyvinyl cage (below).
Figure 7. Figure displayed no sign of skin irritation in Sprague Dawley rats treated with GO,
placebo and saline on day 1, 14 and 28.

Figure 8. Images for Histopathology data