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Wayne Riekhof
Center, Denver, CO
Chlamydomonas
CO 2
RuBisCO
To Glc-6-P for
3-PG
Starch synthesis
DGDG SQDG
PGM ASQD
2-PG Based on annotation of
Eno
PEP
UDP-SQ
SO3-
UDP-Glc genome version 3.
PK
To TCA for
PDH
G-3-P
Major conclusions:
AA anabolism
Acetyl-CoA PtdOH CDP-DAG
ACCase
Malonyl-CoA
Acyl-ACP Lipid metabolism is
lyso-PtdOH
MCT
Acyl-ACP
Acyl-ACP
simpler than in seed
Malonyl- ACP FAS FAT G-3-P plants.
Free-FA
Plastid
Cytoplasm/ER
??
Isozyme numbers are
G-3-P
Free-FA Acyl-CoA GPAT much smaller than
DGTS
LACS
lyso-PtdOH Arabidopsis.
Acyl-CoA LPAAT
Met AdoMet
DAG
PAH
PtdOH
Glc-1-P
Many potential dual-
P-Etn CDP-Etn
targeted proteins.
Etn PtdEtn ACAT/
CDP-DAG
PDAT
Ins-3-P
Ser
PtdGro PtdIns
TAG
3-PG
Starch synthesis
PGM
2-PG
How to maximize the
PEP
Eno
synthesis of TAG while
PK
Pyr
minimizing the “side
PDH
To TCA for
reactions” of carbohydrate
Acetyl-CoA
ACCase
AA anabolism
and amino acid metabolism?
Malonyl-CoA
Acyl-ACP
MCT
FAS FAT
How to induce this pathway in
Malonyl- ACP
Free-FA
nutrient replete cells?
Plastid
??
Cytoplasm/ER
G-3-P
Free-FA Acyl-CoA GPAT
LACS
lyso-PtdOH
Acyl-CoA LPAAT
ACAT PAH
TAG PDAT DAG PtdOH
1. Production of Acetyl-CoA
atmospheric pCO2:
A. Claisen condensation:
KAS1, KAS2, KAS3
B. Reduction of -keto:
KAR1
C. Dehydration of -hydroxy:
HAD1
Acyl-ACP Acyl-ACP
FAT
FAT
Free-FA
Plastid
?? Chnl
Cytoplasm/ER
Free-FA
LACS
LACS
Acyl-CoA Acyl-CoA
4. Glycerolipid Assembly
-
HO O
In mammalian liver and adipose
O
OH
HO P tissue, GPAT is a major control
G-3-P O point for flux into TAG.
No GPAT homolog is present in
Acyl-CoA GPAT the Chlamydomonas genome.
-
HO O
1
O
OH A recently identified
O P
R
lysophospholipid acyltransferase
O
O
lyso-PtdOH enzyme with LPAAT activity was
recently described in Yeast
Acyl-CoA LPAAT (Riekhof et al., JBC, 2007, and
others.)
2
PtdOH
R
O
This gene has a homolog in
O O
- Chlamydomonas, and may
R
1
O
O
P
OH represent the unidentified LPAAT
O
activity.
O
5. Formation of Triacylglycerol
R
2
PtdCho DAG
O 2
R
- O
O O
CH3
PLC/CT
O O +
1 P N O
R O CH3 OH
O CH3 1
O
R
O
O
To avoid coalescence
upon seed desiccation,
the surface of the lipid
droplet in oilseeds is
coated in oleosin
proteins.
No known lipid droplet
surface proteins have
been identified in the
Chlamydomonas genome.
Protein composition of
algal lipid droplets is
unknown.
Starch Versus TAG Accumulation
Future Research