MEASUREMENT OF

PROTOZOAN CYSTS AND

HELMINTHIC OVA
Rodriguez, Merriam Jude
Identification of Protozoa and
other parasites
Location
Particular type of organism (host)
Morphological structure
Size
 Fig.2. Ocular Micrometer

Fig.1. Microscope

Fig.3. Stage Micrometer

Objectives

Calibrate the microscope using a stage

micrometer
Accurately measure helminths,
protozoans and other parasites using an
ocular
Materials

Ocular/Stage Micrometer
Immersion Oil
Lens Paper
Binocular Microscope
Prepared Slides
 Materials
Giardia lamblia trophozoite
(OIO)
Schistosoma ova
(LPO and HPO)
Balantidium coli trophozoite and cyst
(LPO and HPO)
Clonorchis sinensis ova
(LPO and HPO)
Computations
Calibration Factor= Stage Micrometer
Ocular Units
 Final Measurement= (Actual) X (Calibration Factor)
 Stage micrometer: O.1 MM
Ocular units= 10
CF= 0.1 mm 1000 µm
X = 10 µm
10 1 mm

Fig. 4 Calibrating under LPO (100 X)

 Stage micrometer: O.05 mm
Ocular units= 20
CF= 0.05mm 1000 µm
X = 2.5 µm
20 1 mm

Fig. 5 Calibrating under HPO (400 X)

 Stage micrometer: O.01 mm
Ocular units= 10
CF= 0.01mm 1000 µm
X = 1 µm
10 1 mm
Fig. 6 Calibrating under HPO (1000 X)
Table 1. The Ocular Unit, Stage Micrometer and Calibration Factor at different magnifications
LPO HPO OIO
(100 X) (400 X) (1000 X)
Ocular Unit 10 20 10

Stage Micrometer 0.1 mm 0.05 mm 0.01 mm

Calibration Factor 10 µM 2.5 µM 1 µM

Sample by Group 1
Actual Size:
Length= 15
Width= 9

Figure 7. Giardia lamblia trophozoite (1000X)

Sample by Group 1

Actual Size: Actual Size:

Length= 5 Length= 18
Width= 5 Width= 19

Figure 8. Schistosoma japonicom ova (400X) Figure 9. Schistosoma japonicom ova (1000X)
Sample by Group 1

Actual Size: Actual Size:

Length= 7 Length= 26
Width= 5 Width= 21

Figure 10. Balantidium coli cyst (400X) Figure 11. Balantidium coli cyst (1000X)
Sample by Group 1

Actual Size: Actual Size:

Length= 3 Length= 11
Width= 1 Width= 4
Figure 12. Clonorchis sinensis ova (400X) Figure 13. Clonorchis sinensis ova (1000X)
 Data (Results)
Table 2. Actual and Theoretical size measurement and of various Parasites
RESULTS [Size (µM)]

PARASITES 1 2 3 4 THEORETICAL SIZE (µM)

100X 400X 1000X 100X 400X 1000X 100X 400X 1000X 100X 400X 1000X

Length N/A N/A 15 - - - - N/A 10 N/A N/A 14.2 12-15

Giardia lamblia
trophozoite
Width N/A N/A 9 - - - - N/A 6 N/A N/A 8 5-9

Length 50 45 N/A - - - - 75 N/A 68 - N/A 70-100

Schistosoma japonicum
ova
Width 50 47 N/A - - - - 47.5 N/A 40 - N/A 50-70

30-200
Length 70 65 N/A - - - - - N/A - - N/A
Balantidium coli
trophozoite 40-70
Width 50 52.5 N/A - - - - - N/A - - N/A

Length - - - - - - 67.5 - N/A 60 - N/A 50-70

Balantidium coli cyst

Width - - - - - - 62.5 - N/A 41 - N/A 50-70

Length 30 27.5 N/A - - - - 32.5 N/A 24 - N/A 27-35

Clonorchis sinensis
ova
Width 10 10 N/A - - - - 17.5 N/A 14 - N/A 11-20

*N/A= Not Applicable For Group 2 data= didn’t get any

(-)= No data
Worksheet
 The calibration factor is a value that the reading has to
be multiplied with in order to get the corresponding
ground-truth reference. This value is the actual size we
see on the organism. This value decreases as the
magnification increases because the higher the
magnification, the closer we are to the actual size of the
organism/ specimen (Etares, 2018).
 CONCLUSION
Calibrate the microscope=
AVOID misidentification, false results
REFERENCES
 Adam, R. Biology of Giatdia Lamblia. Clinical Microbiology Reviews. 14 (3).
447-475. http://doi.org/10.1128/CMR.
 Encyclopedia Reference of Parasitology. 2001. Schistosoma. [WWW
Document]. URL http://parasite.org.au/para-site/text/schistosoma-
text.html. (01.22.18).
 Global Health. 2017. Clonorchiasis. [WWW Document]. URL
https://www.cdc.gov/dpdx/clonorchiasis/index.html. (01.22.18).
 Lagana, S. 2010. Ciliates affecting man. [WWW Document]. URL
http://www.atlas protozoa.com/Balantidium_coli.php. (01.22.18).