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SPECIFICATION SHEET

Ion OneTouch™ System

The Ion OneTouch™ System


Fast, automated template prep for the Ion PGM™ sequencer

The Ion OneTouch™ System simplifies


the workflow for the Ion Personal
Genome Machine™ (PGM™) system by
providing an automated solution for
scalable and reproducible template
preparation. As a result, the Ion
OneTouch™ System streamlines the
overall Ion semiconductor sequencing
workflow, yielding significant time
savings and increasing sequencing
productivity.

The Ion OneTouch™ System:

• Integrates multiple manual System components


template preparation workflows
into a single system The Ion OneTouch™ System comprises two modules: the Ion OneTouch™
Instrument and the Ion OneTouch™ ES (enrichment system).
• Enables parallel processing of At the heart of the Ion OneTouch™ Instrument are three breakthrough technolo-
multiple samples per day through gies that enable automated delivery of templated Ion Sphere™ particles. The first
modular design is the reaction filter that creates millions of microreactors in which clonal ampli-
fication occurs. The second is the revolutionary in-line PCR amplification plate
• Supports scalable template that enables thermal cycling of the microreactors. The third is the integrated
preparation for the full range centrifuge, which recovers the templated Ion Sphere™ particles. Combined, these
of Ion PGM™ sequencing chips first-in-class technologies deliver massively parallel clonal amplification and
recovery with walk-away operation—all in a small benchtop footprint.
• Reduces time and cost of template
preparation The Ion OneTouch™ ES employs proven magnetic bead technology to isolate
template-positive Ion Sphere™ particles that can be loaded directly onto the
• Fits easily on any benchtop with its Ion semiconductor chip—delivering automated, highly reproducible enrichment
with every run.
small instrument footprint

Automated workflow

The Ion OneTouch™ System automates multiple manual steps to provide


template preparation, including enrichment, in approximately four hours of total
time with only minutes of hands-on time. This is a 60% reduction of total time
compared to standard methods, enabling preparation of multiple samples per
day and significant time savings.
Figure 1. Ion OneTouch™ System Workflow

01 02 03 04 05 06
Set up instrument Set up amplification Amplify Set up ES Retrieve sample Enrich
Load amplification reaction Load amplification Load enrichment Recover sample As noted in step 2,
plate and tubes Combine library, reaction and filter reagents into the from OneTouch™ load sample in
containing oil/ amplification mix, assembly. 8-well strip (provided), Instrument. one well and initiate
recovery solution. enzyme, and Ion Initiate run from and attach liquid Remove recovery enrichment run.
Prepare centrifuge. Sphere™ particles. touch screen. handling tip provided. solution.
Attach filter. Resuspend.

For both the Ion OneTouch™ System components


Instrument and the Ion OneTouch™ ES,
The Ion OneTouch™ Instrument is The Ion OneTouch™ Instrument also
instrument setup, sample preparation,
scalable and supports Ion 314™ chips, supports processing of multiplexed
and run initiation steps are simplified
Ion 316™ chips, and Ion 318™ chips.* libraries, reducing the time and cost
for maximum efficiency and through-
This enables automated preparation of template preparation for both DNA-
put (Figure 1).
and fast sequencing of 10 Mb to 1 Gb* and RNA-based applications.
for maximum flexibility in project type
Modular design
and size.
The modular design of the
Ion OneTouch™ System enables Ion OneTouch™ System specifications
parallel template processing with System Ion OneTouch™ System (Cat No. 4470001) includes:
sample sequencing on the Ion PGM™ • Ion OneTouch™ Instrument
sequencer. This reduces the time from • Ion OneTouch™ ES
sample to sequence to a single day Dimensions • Ion OneTouch™ Instrument:
and enables processing of multiple and weight (12 in x 16 in x 14 in, 23 lb; 30 cm x 41 cm x 36 cm, 10.4 kg)
• Ion OneTouch™ ES:
samples per day. The combination of (9.5 in x 12.5 in x 11 in, 12 lb; 24 cm x 32 cm x 28 cm, 5.4 kg)
the Ion PGM™ sequencer and the Ion
System run time 4 hours total time, minutes of hands-on time
OneTouch™ System maximizes the
Throughput  upports template preparation for Ion 314™ chips,
S
speed of each experiment and mini- Ion 316™ chips, and Ion 318™ chips*
mizes the overall time to completion
Library types Supports template preparation with a broad range of libraries
for each project. The modular design used for various applications:
also allows for QC of Ion Sphere™ • Genomic DNA (fragment and mate-paired)
particles, to maximize experimental • Amplicon
• RNA (cDNA)
control and the success of each
experiment. Operating environment Temperature: 15-25°C; humidity: 20–80%, noncondensing
Consumables Ion OneTouch™ System Template Kit (Cat No. 4468660)
Power requirements 110/220 V (US/International)
Multiplexing Up to 384 barcoded libraries for DNA- or RNA-based applications

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
*The content provided herein may relate to products that have not been officially released and is subject to change without notice.
© 2011 Life Technologies Corporation. All rights reserved. The trademarks mentioned herein are the property of Life Technologies
Corporation or their respective owners.
Printed in the USA. CO23763 1111

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