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Thin-Layer Chromatographic
Method
Thin-Layer Chromatographic
Method
Thin-Layer Chromatographic
Method
Thin-Layer Chromatographic
Method
Thin-Layer Chromatographic
Method
Thin-Layer Chromatographic
Method
MYCOTOXINS: Determination of
Aflatoxin, Ochratoxin A, and
Zearaleone in Mixed Feeds with
Detection by Thin Layer
Chromatography or High Performance
Liquid Chromatography
Feed, Food, Proxima AOAC Official Method TECATOR
Feedstuff, te Ref. HOWELL & TAYLOR: J. ASSOC.
965.16 S,
Dairy analysis Sampling
OFF. ANAL. of Animal64, No.
CHEM.vol. ANKOM,
Products, & Feed
6, Spectrop
Juice, Meat & Other 198 ho
Meat tests: AOAC Official
1 Method tometer,
Products, Fish Sampling, 965.16 etc.
& Moisture, C. Sampling ,of Animal
Fish Products, Protein, Fat, Feed
etc.
Fats C.
& Oil, Plants, Fiber, Ash, AOAC Official Method
Fruits, Grains, and Salt as NaCl, 950.02
Fertilizer etc. Acid Animal
Detergent Feed
Fiber, Neutral
Detergent Page Preparation
3 of 42 of Analytical 92/10/2011
Fiber, Acid Sample
Detergent
Lignin, AOAC Official Method
Nitrate- 950.02
Nitrogen, Animal
Digestible Feed
Protein AOAC Official Method
Determinatio 945.14
n, Sampling of Cereal
Determinatio Adjuncts
n
of AOAC Official Method
Free 935.25
Ammonia, Sampling of
Determinati Malt
on of
Urease AOAC Official Method
Activity, 935.26
Specific Ma
Gravity, Urea, lt
Pepsin
Digestibility, Preparation of Test
Dietary Fiber, Sample
Acid Insoluble
Ash, Invert AOAC Official Method
Sugar, 922.01
Sampling of
Plants
CORN BREAKAGE
SUSCEPTIBLITY
Ref. AACC 55-20 page 1-2,
1995
,reviewed 10-26-
94
Distillation or Drying
Method
Gravimetric
Method
Determination of Common
Salt
Ref. AACC,40-61A,PAGE 1-
2
Digestible Protein
Determination
Ref. AOAC,
1990
Determination of Free
Ammonia
Ref. AOAC,
1984
Determination of Urease
Activity
Ref. AOAC,
1990
Preparation of Laboratory
Sample
Preparation of
Sample
Kjeldahl
Method
First Action
1955
Acid Hydrolysis
Method
Page 9 of 42 92/10/2011
Acid Hydrolysis
Sampling of
Eggs
Enzymatic-Gravimetric
Method
(Phosphate
Buffer)
Gravimetric
Method
General
Instructions
Preparation of
Sample
Preparation of Test
Portions
Procedu
re
Procedu
re
Titrimetric
Method
Gravimetric
Method
Kjeldahl
Methods
Modified
Mojonnier
Ether Extraction
Method
Page 13 of 42 92/10/2011
AOAC Official Method
990.19
Solids (Total) in
Milk
Gravimetric
Method
AOAC Official Method
920.124
Acidity of
Cheese
Titrimetric
Method
Page 14AOAC
of 42 Official Method 92/10/2011
969.18
Refractive Index of
Butterfat
Refractometer
Method
Liquid Chromatographic
Method
Refractometer
Method
Non-Enzymatic-Gravimetric
(Phosphate
Buffer)
Enzymatic–Gravimetric
Method, Phosphate Buffer
Gas Chromatographic
Method
Gas Chromatographic
Method
Preparation of Test
Sample
Vacuum Oven
Method
Preparation of Methyl
Esters
Boron Trifluoride
Method
Titration
Method
Acetylation
Method
Page 19 of 42 92/10/2011
Titrimetric
Method
Wijs
Method
Titrimetric
Method
Titration
Method
Page 20 of 42 92/10/2011
AOAC Official Method
920.157
Melting Point of Fats and Fatty
Acids
Capillary Tube
Method
Chromatographic
Method
Direct Saponification-
Gas
Chromatographic
Method
Atomic Absorption
Spectrophotometry
Page 21 of 42 after Dry Ashing 92/10/2011
Direct Graphite
Furnace
Atomic Absorption
Spectrophotometric
Metho
d
Atomic Absorption
Spectrophotometric
Metho
d
Multielement
Method
Atomic Absorption
Spectrophotometric
Metho
d
Atomic Absorption
Spectrophotometric
Metho
d
Atomic Absorption
AOAC Official Method
973.36
Titanium in
Cheese
Spectrophotometric
Method
Spectrophotometric
Molybdovanadate
Metho
Feed, Food, Vitamin DETERMINATION d OF VITAMIN A HPLC
Herbal s: AND TOCOPHERYL ACETATE IN
& Drugs, Vitamin AOAC
FOOD ANDOfficial
FEED Method
PREMIXES
Feedstuff, Dairy A, 924.07
Products, Juice, Vitamin Sulfur (Total)
Ref. ROCHE METHODS in Fruit
VITAMIN
Meat & Meat E, Products
Products, Fish & Vitamin A and E in FEED and FOOD Ref.
Fish Products, D, Gravimetric
Fats Vitamin B1, ROCHE Method
METHODS
& Oil, Plants, Vitamin B2,
Fruits, Grains, and Vitamin B6, DETERMINATION of VITAMIN D3
Fertilizer etc. Folic Acid, in
Vitamin C, PREMIXE
Niacin, S
Nicotinamid,
Vitamin k, Ref. ROCHE
Beta METHODS
Carotein,
Apo- Determination of Vitamins B1, B2,
Carotein, B6, Folic Acid and PP in Feed and
Vitamin B12, Food Premixes
Biotin, etc.
Ref. ROCHE
Page 24 of 42METHODS 92/10/2011
Determination of Vitamin K3 in
Premixes and Vitamin Concentrates
with the Aid of
HPL
C
Ref. ROCHE
METHODS
Determination of Ascorbic
acid
in Complete Feeds, Premixes and
Vitamin
Concentrat
es
Ref. ROCHE
METHODS
Determination of Vitamin
B1 in Complete Feeds
Ref. ROCHE
METHODS
Determination of Vitamin
B1
in Complete Feeds and
Premixes
Ref. ROCHE
METHODS
Amino Acids
Analysis
ACID-STABLE AMINO
ACIDS
SULFUR AMINO
ACIDS
Feed, Food, Pesticide AOAC Official Method GC, GC-
Herbal Residues Ref. AACC,METHOD
985.22 07- MS
& Drugs, 11,
Organochlori
Page 26 of 42 92/10/2011 &
Vegetable 1995(9TH
ne HPLC
ADDITION)
TRYPTOPHAN-ALKALINE
HYDROLYSIS
Liquid Chromatographic
Method with Ultraviolet
Detector
CIPRFLOXA
CI N AOAC Official Method
993.32
Multiple Sulfonamide>
Residues
ENTRIFLOXA in Raw Bovine
C IN Milk
Liquid Chromatographic
Method
Chloramphenic
ol
Ref: HPLC Method G.
Lunn
Ronidazo
le
Water & Soil pH, Ref:
AOACHPLC-Method
Official MethodG. General
Particle Lunn
955.37
Fractionatio Specific Gravity of
n, SOIL Neomyci
Water
ORGANIC ne
MATTER Ref: HPLC-Method
Pycnometer G.
Loss on Method
Lunn
Ignition,
PHOSPHATE AOAC Official Method
S, 973.40
ELECTRICAL Specific
CONDUCTAN Conductance of
C E, TOTAL Water
SOLIDS
, TOTAL AOAC Official Method
ALKALINIT 973.41
Y, TOTAL pH of
HARDNES Water
S
,
DISSOLVE AOAC Official Method
D OXYGEN, 973.42
SULPHATE Acidity of
S Water
,
CHLORIDES Page 29 of 42 Titrimetric 92/10/2011
, Method
CARBONAT
ES AND AOAC Official Method
BICARBONA 973.43
T ES Alkalinity of
Water
, TOTAL (BOD) of
ORGAN Water
IC
MATTER Incubation
(OXYGE Method
N
CONSUME AOAC Official Method
D) 973.45
Oxygen (Dissolved) in
Water
Titrimetric
Methods
Titrimetric
Methods
Infrared Analyzer
Method
Kjeldahl
Method
Mercuric Nitrate
Method
Colorimetric
Method
Iodometric
Method
Titrimetric
Method
Gravimetric
Method
Atomic Absorption
Spectrophotometric
Metho
d
Atomic Absorption
Spectrophotometric
Metho
d
Photometric
Method
Turbidimetric
Method
Liquid Chromatographic
Method
PH VALUE (HYDROGEN
ION CONCENTRATION)
Particle
Fractionation:
SOIL ORGANIC
MATTER Loss on
Ignition
PageRef.
33 ofSoil,
42 Water & Plant 92/10/2011
Analysis,
198
1
PHOSPHATE
S
Ref. Water Analysis Std.
Methods
TOTAL
SOLIDS
Ref. Water Analysis Std.
Methods
TOTAL
ALKALINITY
Ref. Water Analysis Std.
Methods
TOTAL
HARDNESS
DISSOLVED
OXYGEN
SULPHATE
S
CHLORIDE
S
Ref. Water Analysis Std.
Methods
Page 34 of 42 92/10/2011
CARBONATES AND
BICARBONATES
Ref. Water Analysis Std.
Methods
Microbiology Department ( List of analysis )
Material Types of Standard Description
s/ Tests/Priorities Specifications/ of
Product Measured
Total rangeCount
Bacterial of Techniques
FDA- Bacteriological Equipments
Incubator CO2
Tested Measurements Analytical used used
(Hera-Cell)
Manual.2001, 8th edition/ Stuart Scientific
colony counter
Aerobic Plate count
Anaerobic Plate count Chapter 3 Incubator 32oC , 37oC
Food -
Meat & Direct microscopic count
meat Lactic acid bacterial
count Total Standard Methods for the
products staphylococcus count examination of Dairy products
Poultry & Spore- formers count -
16th ed.1992
eggs Fish & Laboratory Pasteurization count
fish products
juice & soft
drinks, canned Total Bacterial Count(MPN)
foods, cereals IDEXX Simplate (approved by
& FDA)
bakery Total Coliform Count
products
Coliform plate count
Dairy & FDA- Bacteriological
dairy Analytical
products Manual.2001, 8th
edition/ Chapter 3
Feed & grains Coliform and Fecal coliform count
Water
Page 35 of 42 29/11/2011
Plants, soil, and
Fertilizers
(MPN) IDEXX Colilert-18 (approved
by FDA)
Isolation of
Pathogenic
Bacteri
a FDA-Bacteriological
Analytical manual 8th ed.
Isolation & identification of 1998.AOAC- USA
Bacillus cereus Chapter 14&15
Chapter 12
Isolation & identification of
Staph.aureus Chapter 16
Page 37 of 42 29/11/2011
Gross examination of feces Veterinary Clinical . Microscope (Nikon)
Direct wet smear Parasitology-5th edition (1978)
Formol detergent sedimentation
Feces Floatation
Baerman's
Method
Nematode
ELISA Machine
ELISA Titer Assay Biochek Poultry Immunoassay (Multiskan EX)
(ELISA)
Blood
(4000-8000) IBD range
Infectious Bursal Ab test kit
(Avian serum)
(5500-9500) REO range
Reovirus Ab test kit
range (500-1500)
Mycoplasma synoviae Ab
test kit Media and reagents
MS range (1000- (Oxoid)
OIE-method 2000
3000) Blood culture Page 38 of 42 29/11/2011
Complete Blood Count (CBC)
According to cel-dyn 3700 CEL-DYN 3700
Page 39 of 42
29/11/2011
Mycotoxins ELISA Machine
(Multiskan EX)
Aflatoxin B1,M1,Total
T-2 toxin
Fumonisin
According to the
Feeds Zearalenon manual described by
RIDASCREEN
and Citrinin Enzyme immunoassay for
grains the quantitative analysis of
Ochratoxin Mycotoxins
Dondeoxynivalenol
Real time PCR
References
Logan J, Edwards
GMO K, Saunders N
Food and feed (editors) (2009).
Real-Time PCR:
Current Technology
and Applications.
Caister Academic
Press. ISBN 978-1-
904455-
39-4.
Page 40 of 42
29/11/2011
African Horse Sickness
Manual of ELISA Machine
(Multiskan EX)
Equine Infectious Diagnostic
Tests and
Anemia Equine Viral Vaccines
for
Arteritis Terrestrial
Hors Animals
e
Equine Piroplasmosis 2004
Contagious Equine Section 2.5
Metritis Dourine
Glanders
Equine Influenza
Salmonella typhimurium
Page 41 of 42
29/11/2011
Brucella ssp.
Foot and Mouth Disease Virus
Bovine Viral Diarrhoea Virus
Mycobacterium
paratuberculosis Malignant
Conventional
catarrhal fever virus
Animal and Mycoplasma, contagious Real-time PCR
poultry bovine pleuropneumonia
diseases Mycoplasma, contagious
caprine pleuropneumonia
Rift valley fever
Toxoplasma gondii
Coxiella burnetii &
Chlamydophila abortus
Newcastle Disease Virus
Lento Newcastle Disease
Virus Velo Infectious
Bronchitis Virus Infectious
Bursal Disease Virus Avian
Influenza Virus Mycoplasma
gallisepticum / synoviae
Page 42 of 42
29/11/2011