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Keywords
Calcium fortification Pleurotus ostreatus Mushroom production Agricultural lime
Eggshell Oyster shell
III. Substrate Formulation, Preparation and Table 1 Influence of varying concentrations of Ca sources on the
Enrichment yield and biological efficiency of P. ostreatus
Calcium Concentration Total yield (g) Biological
sources (%) efficiency (%)
Seven parts of composted rice straw were combined with
three parts of sawdust and one part of rice bran. The mixture Agricultural 0 150.60 ± 4.22b 28.69 ± 0.93b
lime
was divided and supplemented with different concentrations 2 179.20 ± 14.55 ab
33.52 ± 2.98ab
(2, 4, 6, 8 and 10%) of the Ca sources and the unsupple- 4 177.40 ± 26.58ab 33.70 ± 5.18ab
mented mixture served as control. Enriched substrates were 6 165.80 ± 19.66 ab
31.27 ± 3.50ab
compressed in polypropylene bags and sterilized for 1 h. 8 191.80 ± 19.10a 35.57 ± 2.99a
The bags were allowed to cool down and each bag was 10 191.20 ± 18.14 a
35.63 ± 3.75a
aseptically inoculated with grain spawn. Initial weights of Eggshell 0 150.60 ± 4.22 b
28.69 ± 0.93b
the bags were obtained and were incubated at 30 °C tem- powder
2 170.80 ± 28.66ab 31.88 ± 5.28ab
perature with 80–90% relative humidity until mushroom
4 169.20 ± 19.07 ab
31.51 ± 3.43ab
formation.
6 197.60 ± 19.05 a
37.43 ± 5.54a
8 154.60 ± 17.39b 29.89 ± 3.52b
IV. Harvest and Assessment of Biological Efficiency
10 143.20 ± 10.20 b
27.26 ± 2.51b
The duration of the period from inoculation to full mycelial Oyster shell 0 150.60 ± 4.22a 28.69 ± 0.93a
powder
colonization, primordial formation and fruiting body devel- 2 172.40 ± 18.23 a
32.82 ± 3.58a
opment were recorded. The fruiting bodies of P. ostreatus 4 173.20 ± 25.17 a
33.04 ± 4.42a
were then harvested, weighed and air-dried until the 3rd 6 168.00 ± 8.22a 32.07 ± 1.50a
flush. Yield and biological efficiency (BE) were recorded. 8 153.60 ± 20.38 a
29.18 ± 3.86a
10 168.40 ± 37.96 a
31.98 ± 7.43a
V. Determination of Mineral Contents in Fruiting The displayed values are means and SD. Treatment means in each Ca source
Bodies with different letter of superscript in each column are significantly different
from each other at 5% level of significance using LSD
Dried fruiting bodies of P. ostreatus were ground using a
blender and analyzed for elemental composition using
Thermo EDX system installed in Hitachi SU1510 scanning
electron microscope. Other elements such as Mg, P, K, Na,
Si, Cl, and S were also determined.