Methods For HPTLC Plate_20180404_HPTLC Psidium derivated Fitofar visionC ATS

Analysis: Methods For HPTLC

Plate_20180404_HPTLC Psidium derivated
Fitofar
Path: Home/Demo Project/UIN MALANG
Based on method: Methods For HPTLC Plate
Created 04-Apr-2018 11:08:51 visionCATSuser
Modified 04-Apr-2018 11:14:19 visionCATSuser
Last HPTLC log 04-Apr-2018 11:14:19 Analysis modified

Track Vial ID Description Volume Position Type

1 1 QUAERCETIN 2.0 µl N/A Sample
2 2 Ekstrak Etanol 96% Daun Jambu Biji 1 2.0 µl N/A Sample
3 3 Ekstrak Etanol 96% Daun Jambu Biji 3 2.0 µl N/A Sample
4 4 Ekstrak Etanol 96% Daun Jambu Biji 5 2.0 µl N/A Sample
5
6
7
8
9
10
11
12
13
Notes

A track m ark ed with m eans: the application type is overridden in som e evaluation(s).

System setup:
Software Server desktop-clq9jc2, version 2.4.17207.2
Chamber N/A
Derivatization spray N/A
Nanomat N/A
Visualizer 2 S/N:241214

Chromatography

Plate layout:
Stationary phase Merck, HPTLC plates silica gel 60 F 254
Plate format 100.0 x 100.0 mm
Application type Spot
Application Position Y: 8.0 mm, length: 0.0 mm, width: 0.0 mm
Track First position X: 20.0 mm, distance: 5.0 mm
Solvent front position 95.0 mm
Notes

Application 1 - Nanomat:
Notes

Development 1 - Chamber:

04-Apr-2018 11:14:19 - visionC ATSuser Id: rAxwVyI= 1-8

Methods For HPTLC Plate_20180404_HPTLC Psidium derivated Fitofar visionC ATS
Tank TTC 20x10
Mobile phase
Saturation time 20 min
Use saturation pad true
Use smartALERT false
Volume front through 10 ml
Volume rear through 20 ml
Drying time 5 min
Drying temperature Room temperature
Notes

Derivatization 1 - spray:
Reagent name
Reagent preparation
Heating none
Notes

Take image derivatized plate 1a - Visualizer (S/N: 241214):

Quality Enhanced
RT White auto capture, Auto, level 85 %, Band
R 366 auto capture, Auto, level 85 %, Band
Documentation step label
Notes

System suitability tests:

SST settings:
SST tracks

Data acquisition

Application 1 - Nanomat:
Executed 04-Apr-2018 11:09:11 visionCATSuser

Development 1 - Chamber:
Executed 04-Apr-2018 11:09:14 visionCATSuser

Derivatization 1 - spray:
Executed 04-Apr-2018 11:09:15 visionCATSuser

Take image derivatized plate 1a - Visualizer (S/N: 241214):

Executed 04-Apr-2018 11:09:16 visionCATSuser

RT White Derivatized, RemTransVis

04-Apr-2018 11:14:19 - visionC ATSuser Id: rAxwVyI= 2-8

Methods For HPTLC Plate_20180404_HPTLC Psidium derivated Fitofar visionC ATS

Ex posure 0.024 s
Contrast 1
Norm alized ex posure Disabled
Clarify Disabled
W hite balance 1.00, 1.00, 1.00

R 366 Derivatized, Remission366

Ex posure 3.143 s
Contrast 1
Norm alized ex posure Enabled
Clarify Disabled
W hite balance 1.00, 1.00, 1.00

Evaluation 1 :
Validated false
Step Take image derivatized plate 1a
Notes

04-Apr-2018 11:14:19 - visionC ATSuser Id: rAxwVyI= 3-8

Methods For HPTLC Plate_20180404_HPTLC Psidium derivated Fitofar visionC ATS

Definition:
References:
Samples:
Vial ID Amount Volume solution Reference amount Related to
1 0.00 mg 0.00 ml 0.00 mg
2 0.00 mg 0.00 ml 0.00 mg
3 0.00 mg 0.00 ml 0.00 mg
4 0.00 mg 0.00 ml 0.00 mg

Integration parameters:
Bounds [0.000,1.000]
Smoothing Savitzky–Golay of order 3 and window 7
Baseline correction Lowest slope with noise 0.05
Profile subtraction None
Peaks detection Gauss with sensitivity 0.1, separation 1 and threshold 0.1

Scan:
Wavelength 366 nm

Track 1:
Type Sample
Vial ID 1
Description QUAERCETIN
Volume 2.0 µl

0.10
4

0.08

0.06
AU

0.04

3
1 2
0.02

0.00
0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1.0
Rf

Peak Start Max End Area Manual Substance

# Rf H Rf H % Rf H A % peak Name
1 0.062 0.0014 0.107 0.0201 13.11 0.134 0.0022 0.00089 11.95 No
2 0.134 0.0022 0.160 0.0197 12.84 0.214 0.0042 0.00077 10.30 No
3 0.513 0.0005 0.530 0.0249 16.17 0.542 0.0016 0.00018 2.39 No
4 0.856 0.0209 0.924 0.0890 57.88 0.957 0.0248 0.00561 75.36 No

Track 2:
Type Sample
Vial ID 2
Description Ekstrak Etanol 96% Daun Jambu Biji 1
Volume 2.0 µl
04-Apr-2018 11:14:19 - visionC ATSuser Id: rAxwVyI= 4-8
Methods For HPTLC Plate_20180404_HPTLC Psidium derivated Fitofar visionC ATS
0.150

0.125 1

0.100

0.075
AU

0.050

0.025

0.000
0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1.0
Rf

Peak Start Max

End Area Manual Substance
# Rf H Rf
% RfH H A % peak Name
1 0.842 0.0109 0.934 0.1153 100.0 0.964 0.0256 0.00676 100.0 No
0 0

Track 3:
Type Sample
Vial ID 3
Description Ekstrak Etanol 96% Daun Jambu Biji 3
Volume 2.0 µl

0.150
3
0.125

0.100

0.075
AU

0.050

1
0.025 2

0.000
0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1.0
Rf

Peak Start Max End Area Manual Substance

# Rf H Rf H % Rf H A % peak Name
1 0.122 0.0055 0.145 0.0229 13.84 0.160 0.0069 0.00037 4.54 No
2 0.659 0.0000 0.664 0.0145 8.79 0.668 0.0005 0.00007 0.92 No
3 0.844 0.0160 0.925 0.1280 77.37 0.963 0.0288 0.00766 94.54 No

Track 4:

04-Apr-2018 11:14:19 - visionC ATSuser Id: rAxwVyI= 5-8

Methods For HPTLC Plate_20180404_HPTLC Psidium derivated Fitofar visionC ATS
Type Sample
Vial ID 4
Description Ekstrak Etanol 96% Daun Jambu Biji 5
Volume 2.0 µl

0.150

0.125

0.100

0.075
AU

0.050

0.025

0.000
0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1.0
Rf

Peak Start Max End Area Manual Substance

# Rf H Rf H % Rf H A % peak Name

Scan:
Wavelength RT White

Track 1:
Type Sample
Vial ID 1
Description QUAERCETIN
Volume 2.0 µl

0.08
1

0.06

0.04
AU

0.02

0.00
0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1.0
Rf

Peak Start EndMax Area Manual Substance

# Rf H %Rf Rf H
H A % peak Name
1 0.036 0.0013 0.103 0.0681 100.0 0.145 0.0020 0.00387 100.0 No
0 0
04-Apr-2018 11:14:19 - visionC ATSuser Id: rAxwVyI= 6-8
Methods For HPTLC Plate_20180404_HPTLC Psidium derivated Fitofar visionC ATS
Track 2:
Type Sample
Vial ID 2
Description Ekstrak Etanol 96% Daun Jambu Biji 1
Volume 2.0 µl

0.035

0.030 1

0.025

0.020
AU

0.015

0.010

0.005

0.000
0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1.0
Rf

Peak Start Max

End Area Manual Substance
# Rf H % Rf Rf H H A % peak Name
1 0.001 0.0000 0.019 0.0282 100.0 0.039 0.0000 0.00064 100.0 No
0 0

Track 3:
Type Sample
Vial ID 3
Description Ekstrak Etanol 96% Daun Jambu Biji 3
Volume 2.0 µl

0.025

1
0.020

0.015
AU

0.010

0.005

0.000
0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1.0
Rf

Peak Start Max

End Area Manual Substance
# Rf H % Rf Rf H H A % peak Name
1 0.001 0.0000 0.020 0.0202 100.0 0.044 0.0000 0.00055 100.0 No
0 0
04-Apr-2018 11:14:19 - visionC ATSuser Id: rAxwVyI= 7-8
Methods For HPTLC Plate_20180404_HPTLC Psidium derivated Fitofar visionC ATS
Track 4:
Type Sample
Vial ID 4
Description Ekstrak Etanol 96% Daun Jambu Biji 5
Volume 2.0 µl

0.008

0.006

0.004
AU

0.002

0.000
0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1.0
Rf

Peak Start Max End Area Manual Substance

# Rf H Rf H % Rf H A % peak Name

Calibration results:
Results:

Analyst: Reviewer:

04-Apr-2018 11:14:19 - visionC ATSuser Id: rAxwVyI= 8-8