Interference samples

Conjugated bilirubin (mg/dL)

0.0 2.2 4.4 6.7 8.9 11.1 13.3 15.6 17.8 20.0 22.4

Unconjugated bilirubin (mg/dL)

0.0 1.8 3.7 5.5 7.4 9.2 11.0 12.9 14.7 16.6 18.4

Hemoglobin (mg/dL)

0 45 90 135 180 225 270 315 360 405 450

Lipid (Intralipid : %)

0.0 0.02 0.04 0.06 0.08 0.10 0.20 0.40 0.60 0.80 1.00

<70 105 149 188 246 282 515 1,002 1,192 1,652 2,020
Triglycerides (mg/dL)
Triglycerides were measured by Reflotron® Triglycerides of Roche

Lipid (Formazine turbidity unit)

0 218 436 654 872 1090 1308 1526 1744 1962 2180

Interferences by each substance do not occur up to the concentration represented

by mark on CA series. (Clotting assay, Chromogenic assay and Immunoassay)
Interference testing 10.00 Scientific Div.
How to perform Interference testing
Bilirubin
1. Conjugated bilirubin
Test Material : Dissolve 20mg unconjugated bilirubin in 2mL 0.1N NaOH. (1000 mg/dL)
Test Pool A : Add 0.2mL of test material to 10mL pooled plasma with stirring. (20 mg/ dL)
Test Pool B : Add 0.2mL of 0.1N NaOH to 10mL pooled plasma with stirring.
Test Pool A and B are mixed as following,

Concentration (mg/dL) 0 2.0 4.0 6.0 8.0 10.0 12.0 14.0 16.0 18.0 20.0

Test Pool A (mL) 0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1.0

Test Pool B (mL) 1.0 0.9 0.8 0.7 0.6 0.5 0.4 0.3 0.2 0.1 0.0

2. Unconjugated bilirubin
Test Material : Dissolve 20mg ditaurobilibrubin bilirubin in 2mL distilled water. (1000 mg/dL)
Test Pool A : Add 0.2mL of test material to 10mL pooled plasma with stirring. (20 mg/ dL)
Test Pool B : Add 0.2mL of distilled water to 10mL pooled plasma.
Test Pool A and B are mixed as following,

Concentration (mg/dL) 0 2.0 4.0 6.0 8.0 10.0 12.0 14.0 16.0 18.0 20.0

Test Pool A (mL) 0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1.0

Test Pool B (mL) 1.0 0.9 0.8 0.7 0.6 0.5 0.4 0.3 0.2 0.1 0.0

Hemogulobin

Test Material : Hemolysate made by Osmotic shock procedure or Mechanical lysis procedure.
Test Pool A : Add measured volume of hemolysate to 10 mL pooled plasma to make 500
mg/dL hemogulobin.
Test Pool B : Add equivalent volume of saline to 10 mL pooled plasma.
Test Pool A and B are mixed as following,

Concentration (mg/dL) 0 50 100 150 200 250 300 350 400 450 500

Test Pool A (mL) 0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1.0

Test Pool B (mL) 1.0 0.9 0.8 0.7 0.6 0.5 0.4 0.3 0.2 0.1 0.0

PT assay on CA-1500 09.00 Scientific Div.

How to perform Interference testing
Lipemia
Test Material : 20% fat emulsion (Intralipid, Cutter Laboratories)
Test Pool A : Add 0.5 mL of 20 % Intralipid to a 10 mL volumetric flask. Make up to
volume with pooled plasma.
Test Pool B : Add 0.5mL of distilled water to 10mL pooled plasma.

Concentration (%) 0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1.0

Test Pool A (mL) 0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1.0

Test Pool B (mL) 1.0 0.9 0.8 0.7 0.6 0.5 0.4 0.3 0.2 0.1 0.0

5. Lipemia
Test Material : 20% Intralipid, Cutter Laboratories
Test Pool A : Add 0.5 mL of 20 % Intralipid to a 10 mL volumetric flask. Make up to
volume with pooled plasma.
Test Pool B : Identical, except use 0.5 mL saline or Owren`s veronal buffer in place of
the lipemic stock.

Concentration (%) 0 0.02 0.04 0.06 0.08 1.0 0.2 0.4 0.6 0.8 1.0

Test Pool A (mL) 0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1.0

Test Pool B (mL) 1.0 0.9 0.8 0.7 0.6 0.5 0.4 0.3 0.2 0.1 0.0

PT assay on CA-1500 09.00 Scientific Div.