General Microbiology

MICR211 Fall 2010

Lecture 01 3132 MW 8:00AM-9:15 AM PH2 Rm 110

Lab 02 3133 MW 9:30AM-12:15 PM MIC-102A
Lab 04 10631 MW 2:00PM- 4:45 PM MIC-102A

Instructor: Dr. Shehnaz Rehmat

Phone: (562) 985-8148
Email: srehmat@csulb.edu
Office: MIC 206A
Office Hours: Mon: 1:00 – 2:00PM
Wed: 1:00 – 2:00PM
T/Th: 11:00AM (by appointment)

Prerequisites: BIO 211A (grade of C or better), and CHEM 111B

Required Texts: Prescott’s Microbiology, by Willey, Sherwood and Woolverton, 8th

edition, 2010.
Benson’s Microbiological Applications, by Alfred E. Brown, 11th Edition,
Complete version, 2009.

Required Materials (for lab):

Drawing pencils: black, red, green, blue, and violet
Permanent marker (Sharpie or equivalent)
Three ring binder (for lab reports and syllabus pages)
Blue Exam Book for unknown project
Safety goggles
Lab Coat
Closed toed shoes

Recommended (fun) readings:

Deadly Companions: How Microbes Shaped Our History, by D. H. Crawford (2009)
Panic in Level 4: Cannibals, Killer Viruses and Other Journeys to the Edge of Science, by
Richard Preston (2009)
The Deadliest Strain, by J. Coffrey (2008)
Twelve Diseases that Changed our World, I. W. Sherman (2007)
20th CenturyMicrobe Hunters, by R. Krasner (2007)
When Germs Travel: Six Major Epidemics that have invaded America since 1900 and the fears
they have unleashed, by H. Markel (2004)
Deadly Feasts, Tracking the Secrets of a Terrifying New Plague, by Richard Rhodes (1997)
Germs: Biological Weapons and America’s Secret War,, by Judith Miller, Stephen, Engelberg,
& William Broad
 MICROBIOLOGY 211
Reading Assignments and Lecture Schedule – Fall 2010

Date Chapter* Topics

Aug 30 1 Evolution of Microorganisms and Microbiology
Sept 1 2 Microscopy and Specimen Preparation
6 Labor Day - Holiday
8 3 Bacteria and Archaea
13 3 Bacteria and Archaea
15 4 Eucaryotic Cell Struture and Function
20 5 Viruses and Other Acellular Infectios Agents
22 5 Viruses and Other Acellular Infectios Agents
27 EXAM 1 covering Chap’s 1 - 5 and lect. material
29 6 and 7 Microbial Nutrition and Growth
Oct 4 8 Control of Microorganisms in the Environment
6 9, 10 and 11 Microbial Metabolism
11 9, 10 and 11 Microbial Metabolism
13 12 Gene Structure, replication and Expression
18 13 Regulation of Gene Expression
20 EXAM 2 covering Chap’s 6 -13 and lect. material
25 14 Mechanisms of Genetic Variation
27 15 Recombinant DNA Technology
Nov 1 17 Microbial Taxonomy and the Evolution of Diversity
3 30 Microbial Interactions
8 31 Infection and Pathogenecity
10 32 Nonspecific Host Resistance
15 33 Specific Immunity
17 Exam 3 covering Chap’s covered and lecture material
22 34 Antimicrobial Chemotherapy
24 Fall Break – No class
25 -26 THANKSGIVING - Holiday
29 35 Clinical Microbiology and Immunology
Dec 1 36 Epidemiology and Public Health Microbiology
6 40 Microbiology of Food
8 42 Water purification and Sanitary Analysis
13 FINAL EXAM (Comprehensive)

*Required textbook: Prescott’s Microbiology, by Willey, Sherwood and Woolverton, 8th edition, 2010
 MICROBIOLOGY 211 LABORATORY SCHEDULE – FALL 2010
Starting dates Ex.# *Chapter Laboratory Topics **Due Dates
Aug 30 Orientation and Laboratory Safety
7 Ubiquity of Microbial Life Sep 13
24 Slide Culture of Fungi
Sept 1 1 2 Brightfield Microscopy Sep 13
2 Darkfield Microscopy
Sept 6 Labor Day Holiday - - No Class
8 6 4 Protozoa, Algae, and Cyanobacteria Sep 13
8 4 Fungi: Yeasts and Molds Sep 13
13 9 2 Aseptic Technique Sep 20
11 Smear Preparation Sep 20
12 Simple Stain Sep 20
13 Negative Stain Sep 20
15 15 2 Gram Staining Sep 22
17 Acid Fast Staining Sep 22
20 14 Capsule Staining Sep 22
16 2 Spore Staining Sep 22
18 Motility Determination Sep 22
22 19 6 Culture Media Preparation Sep 29
27 10 6 Pure Culture Techniques Oct 6
29 20 Preparation of Stock Cultures Oct 6
22 6 Standard Plate Count and Turbidometric method Oct 6
21 7 Cultivation of Anaerobes Oct 6
Oct 4 29 Temperature and Effects on Growth Oct 11
31 7 pH and Microbial Growth Oct 11
32 Osmotic Pressure and Microbial Growth Oct 11
6 39 17 Morphological Study of Unknowns
11 30 8 Temperature: Lethal Effects Oct 20
33 Ultraviolet Light: Lethal Effects Oct 20
13 Mid term Lab Exam
18 66 14 Mutant Isolation by Replica Plating Oct 27
67 Bacterial Transformation Oct 27
20 40 Cultural Characterstics
41 10 Physiological Characteristics: Biooxidations Nov 8
25 42 10 Physiological Characteristics: Hydrolysis Nov 8
27 43 10 Physiological Characteristics: Misc Tests Nov 8
Nov 1 25 5 Determination of Phage Titer Nov 10
3 70 35 The Staphylococci: Isolation and Identification Nov 17
8 71 35 The Streptococci: Isolation and Identification Nov 17
10 72 35 Gram-negative Intestinal Pathogens Nov 22
46 Enterotube Nov 22
15 36 34 Antimicrobic Sensitivity Testing:Kirby Bauer Method Nov 29
37 8 Evaluation of Antiseptics and Disinfectants Nov 29
17 61 42 Bacteriological Examination of Water Dec 8
62 Membrane Filtration Method Dec 8
60 Standard Plate Count of Milk and Food Dec 8
22 49 29 Isolation of Antibiotic Producer from Soil Dec 8
52 Free-living Nitrogen-Fixing Bacteria Dec 8
24 Fall Break – no lab
25-26 Thanksgiving Holiday No Classes
29 76 35 Differential WBC count Dec 8
77 Blood Grouping Dec 8
78 36 A Synthetic Epidemic Dec 8
Dec 1 58 30 Bacterial Synergism Dec 8
59 Microbial Antagonism Dec 8
6 Check out-Review
8 FINAL LAB EXAM
Exercises from: Benson’s Microbiological Applications by Alfred E. Brown, 11th ed,complete version, 2009
*Chapters from: Prescott’s Microbiology, by Willey, Sherwood and Woolverton, 8th edition, 2010.
** Due dates: Dates when the lab reports are due.
Point Distribution:

Lecture Laboratory
3 midterm exams 300 points Midterm exam 100 points
Final exam 150 points Lab reports 50 points
Research Paper 50 points Unknown Project 50 points
Quizzes 50 points Quizzes (5) 100 points
Final exam 150 points
total 550 points total 450 points
Overall total for course: 1000 points

Exam Makeup policy: One lecture exam may be made up if missed due to illness, accident,
or other compelling circumstances. Instructor must be notified within one week of the
scheduled exam to arrange for a special makeup exam. Evidence of reason for missing exam
must be submitted before makeup exam can be taken.
Lecture and Lab quizzes and practicums cannot be made up.

Final Grades: Scores from lecture exams and all laboratory work are combined and
percentages of total possible points are calculated. Grades will be based on a curve
constructed from total scores.

Incompletes. A grade of "I", (Incomplete) will only be assigned if a student is unable to

complete required course work (not to exceed approximately 300 points) and notifies the
instructor before final grades are due in the Records Office (usually 72 hours after final exam).
Special arrangements must be made to make up work within one year. Without such
notification, a grade of "U" will be assigned.

Withdrawal Policy. Always file a drop form if you do not intend to continue in any course. It is
good insurance against getting a "U" in the course. Also, it is requested that you inform your
lab instructor if you drop the course early in the semester (first two weeks); this will give us the
opportunity of giving your space to someone else.

For details regarding the College of Natural Sciences and Mathematics withdrawal policy, see
complete policy available in college and department offices.

Attendance: Students are expected to attend all scheduled lectures and laboratory sessions.
It is a student’s responsibility to notify of his or her instructor of any absences, and to arrange
make-up of missed work. Students should be aware, however, that opportunities to make-up
missed work is at the discretion of the instructor.

Cheating and Plagiarism: Cheating is defined as obtaining attempting to obtain or aiding

another to obtain academic credit (i.e. a grade) for work by the use of any dishonest,
deceptive, or fraudulent means. This includes sharing or copying while taking an exam or quiz,
or relating to another student prior knowledge of questions or other details of an exam or quiz.
Plagiarism is using the ideas or work of another for a grade without giving credit to the
originator of the idea or work. Plagiarism includes such things as the use of “mail order” term
papers and the copying of another students answers to questions in laboratory reports. See
the CSULB Bulletin for additional information. Disciplinary action for cheating or plagiarism is
at the discretion of the instructor and could include reduction of grade to zero for the work
involved, or dismissal from the course.


 COLLEGE OF NATURAL SCIENCES AND MATHEMATICS
WITHDRAWAL1 PROCEDURE AND TIMELINE
FALL 2010


The conditions under which students may withdraw and the documents which must be submitted
are detailed in University Policy Statement 09-07, and described in the CSULB Catalog. The most
current information on CSULB withdrawals is posted at:
http://www.csulb.edu/depts/enrollment/registration/details.html#anchor1. The CNSM procedure
statement is in part a reminder of some of those provisions, and it will also serve as a statement of
our understanding of some of the terms used in the Catalog. Students are responsible for
initiating the withdrawal procedure.

8/30 – 9/13/10 I. WITHDRAWALS DURING THE FIRST TWO WEEKS OF INSTRUCTION.

Students may withdraw during this period through MyCSULB (until 9/13/10 @
10:00 p.m.) and the course will not appear on their permanent records. After this
period, a “W” will appear on the permanent record of the student.
9/14 – 11/19/10 II. WITHDRAWALS AFTER THE FIRST TWO WEEKS OF INSTRUCTION AND
PRIOR TO THE FINAL THREE WEEKS OF INSTRUCTION.
A. Instructors shall provide the students with an opportunity for demonstration of competence,
relevant to the determination of their final grade in the course, as early as is
reasonable and no later than the end of the seventh week of instruction.

B. The request and approvals shall state the reasons for the withdrawal.

C. During this period of time, withdrawals are permissible only for serious and compelling
reasons. Students should be aware that the definition of “serious and compelling
reasons” as applied by faculty and administrators may become narrower as the
semester progresses. Written approval must be obtained from both the Instructor
and Department Chair.
11/20-12/10/10 III. WITHDRAWALS DURING THE FINAL THREE WEEKS OF INSTRUCTION.
Withdrawals during the final three weeks of instruction are not permitted except
in cases such as accident or serious illness where the circumstances causing the
withdrawal are clearly beyond the student's control and the assignment of an
Incomplete is not practical. Ordinarily, withdrawals in this category will involve
total withdrawal from the campus except that a Credit/No Credit grade or an
Incomplete may be assigned for courses in which sufficient work has been
completed to permit an evaluation to be made. Grade standing in the course will
not be considered, provided the above criteria are completely satisfied. Requests
to withdraw must be accompanied by supporting documentation. Written
approval must be obtained from the Instructor, the Department Chair, and the
Dean.1

1
A Special form (see reverse side of this form or the college or department offices) for withdrawal
during the last three weeks of instruction must be attached to the University’s “Request to
Withdraw from a Class” form prior to seeking the Dean’s signature.

Approved by College August 2008

COLLEGE OF NATURAL SCIENCES AND MATHEMATICS

APPLICATION TO WITHDRAW FROM CLASS

FOLLOWING November 19, 2010

Notice to students:
The official withdrawal procedure in the College of Natural Sciences and Mathematics is
stated on the reverse side of this form. Effective Fall 2009 there are new university-wide
unit limits on withdrawing and repeating courses. Please consult the University Center for
Undergraduate Advising:
http://www.csulb.edu/divisions/aa/grad_undergrad/aac/documents/CRITICALINFORMA
TIONFORCSULBUNDERGRADUATESFINAL_000.pdf ) for the new policy. Please
read carefully before continuing with the withdrawal process.

If you are withdrawing in the final three weeks of instruction (after November 19, 2010):

a. Fill out the University "Request to Withdraw" form. Attach documentation to

substantiate the reason for withdrawing. Documentation must be written and
signed by someone other than yourself. For example, if illness is the reason for
withdrawal, your physician should document this, including all appropriate dates,
in a signed letter.

b. Take the "Request to Withdraw" form and this form to the instructor of each
course you are dropping.

c. If the instructor approves your request to withdraw, deposit materials, including

this completed form, in the Department Office. After evaluation by the Chair, you
must pick up these materials from the Department Office. The department will
not file the forms for you.

d. If permission is granted by the Chair to withdraw, take the approved form to the
College of Natural Sciences and Mathematics Office (FO5-104). Allow one campus
working day for evaluation by the Dean’s Office before you deliver the form to the
Office of Enrollment Services by the published deadline.


TO BE FILLED IN BY THE STUDENT
Name of Student ___________________________I.D. ______________________
Course (Department/Number) ________________CourseTitle _____________________
Telephone Number _________________________Date ___________________
Are you withdrawing from all classes this semester? _________________________
TO BE FILLED IN BY THE INSTRUCTOR

Last Day of Attendance _____________________ Letter Grade to Date ________

Instructor's Signature ______________________ Date ___________________