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Name: Class:

Answer ALL the questions.


Jawab semua soalan

Aim: To Determine the Concentration Of Vitamin C Content In The Sample Of Fruit Juices
Tujuan: Untuk menentukan kepekatan Vitamin C dalam sampel jus buah.

Procedure:
Kaedah:

Friut juice
Jus buah

1. Place 1 ml of 0.1% DCPIP solution into the specimen tube by using 1 ml syringe
Isikan 1 ml larutan 0.1% DCPIP ke dalam tiub specimen dengan menggunakan picagari 1 ml.

2. Fill the 5 ml syringe with 0.1 ascorbik acid.


Penuhkan picagari 5ml dengan asid askorbik 0.1%.

3. Place the needle of the syringe which contains the ascorbic acid into the specimen tube
which contain DCPIP solution.
Masukkan picagari berisi asid askorbik ke dalam tiub specimen yang mengandungi larutan
DCPIP.

4. Mix the ascorbic acid until the DCPIP solution become colourless(decolourised)
Campurkan asid askorbik setitis demi setitis sambil mengacau larutan tersebut dengan jarum
picagari. Terus campurkan asid askorbik sehingga larutan DCPIP menjadi tidak berwarna
(luntur).

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5.Record the volume of ascorbic acid which has been used.
Catatkan isipadu asid askorbik yang telah digunakan.

6. Repeat step 1 to 5 by using lime juice, lemon juice, and calamondin juice.
Ulang langkah 1-5 dengan menggunakan jus limau, jus lemon, dan jus kasturi.

7. Record the volume of juice which is required to decolourised the DCPIP solution.
Catatkan isipadu jus yang diperlukan untuk melunturkan warna larutan DCPIP.

8. Record the result in the table. Calculate the percentage and concentration of vitamin C in
every type of fruit juice .
Catatkan keputusan dalam jadual. Kirakan peratus dan kepekatan vitamin C dalam setiap jus
buah.

Presentation of data

A. Construct a table and record all the data collected from this experiment. Your table should
have the following titles:
Bina satu jadual dan rekodkan semua data yang dikumpulkan dalam eksperimen ini. Jadual
anda hendaklah mengandungi semua aspek berikut:
 Fruit juice
Jus buah
 Volume of fruit juice needed to decolourise DCPIP solution
Isipadu larutan yang diperlukan untuk melunturkan warna DCPIP
 Percentage of vitamin C content (%)
Peratus kandungan Vitamin C
 Concetration of Vitamin C in fruit juice (mg cm )
 Kepekatan vitamin C dalam jus buah (mg cm )
(3 marks)

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B. What is the formula to calculate the percentage of Vitamin C?
Apakah formula untuk mengira peratus vitamin C?

(1 mark)

C. What is the formula to calculate the concentration Vitamin C?


Apakah formula untuk mengira kepkatan vitamin C?

(1 mark)

D. Show all of your calculation that related to the percentage of vitamin c and concentration of
vitamin C in every fruit juice.
Tunjukkan semua pengiraan anda berkaitan dengan peratus vitamin C dan kepekatan Vitamin C
dalam setiap jus buah.
(12 marks)

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E. State the precuition that should be taken during the experiment.
Nyatakan langkah berjaga-jaga yang perlu diambil semasa eksperimen ini.
(1 mark)

F. Give reason for your answer in Question E.


Nyatakan sebab kepada jawapan anda di E.
(1 mark)

G. What would happen to the concentration of Vitamin C in the fruit juice is exposed to the air
for a day?
Apakah yang akan berlaku kepada kepekatan Vitamin C sekiranya jus buah terdedah kepada
udara selama satu hari?
(1 mark)

End of the question

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