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ADAP 2.0: Measurement and Analysis Software for Biochrom Anthos Microplate Readers Tristana von Will Microplate

ADAP 2.0: Measurement and Analysis

Software for Biochrom Anthos Microplate Readers

and Analysis Software for Biochrom Anthos Microplate Readers Tristana von Will Microplate Instrumentation Applications

Tristana von Will Microplate Instrumentation Applications Specialist

Software for Biochrom Anthos Microplate Readers Tristana von Will Microplate Instrumentation Applications Specialist

ADAP Basic

Outline

Key Features ADAP 2.0 Software

ADAP Basic Outline  Key Features ADAP 2.0 Software  How to use ADAP 2.0 Basic

How to use ADAP 2.0 Basic for Basic Measurements

How to set up a method in ADAP 2.0 Plus Software

How to demonstrate ADAP 2.0 Basic and Plus Software

Your questions?

Use the chat function on Go To Meeting to ask questions. All questiosn will be addressed either at the end of the meeting or offline by email.

ADAP 2.0 Software

ADAP 2.0 Software Software is used to remotely control readers for quick measurements with optional upgrade

Software is used to remotely control readers for quick measurements with

optional upgrade to Plus version for data analysis

EZ Read 400

with optional upgrade to Plus version for data analysis EZ Read 400 Anthos 2020 Zenyth 340
Anthos 2020 Zenyth 340
Anthos 2020
Zenyth 340

Zenyth 200

with optional upgrade to Plus version for data analysis EZ Read 400 Anthos 2020 Zenyth 340

Key Features

Key Features Software is used to remotely control readers for quick measurements with optional upgrade to

Software is used to remotely control readers for quick measurements with

optional upgrade to Plus version for data analysis

Easy to use

Easy to copy data into other analysis programs

Quick set up for quick

measurements

Intuitive test (method) setup (ADAP 2.0 Plus)

Windows 7 Compatible

 Quick set up for quick measurements  Intuitive test (method) setup (ADAP 2.0 Plus) 
 Quick set up for quick measurements  Intuitive test (method) setup (ADAP 2.0 Plus) 

Getting Started

Getting Started  A demo procedure exists to allow exploration of the features of ADAP software

A demo procedure exists to allow exploration of the

features of ADAP software without connecting to an

instrument.

 A demo procedure exists to allow exploration of the features of ADAP software without connecting

Starting up ADAP 2.0 Software

Starting up ADAP 2.0 Software Login: Username: sadmin Password: sadmin All ADAP 2.0 modules control user
Starting up ADAP 2.0 Software Login: Username: sadmin Password: sadmin All ADAP 2.0 modules control user

Login:

Username: sadmin Password: sadmin

ADAP 2.0 Software Login: Username: sadmin Password: sadmin All ADAP 2.0 modules control user access at

All ADAP 2.0 modules control user access at different authorisations levels allowing users to work within FDA 21 CFR, part 11 compliance.

control user access at different authorisations levels allowing users to work within FDA 21 CFR, part

Accessing the demonstration mode

Accessing the demonstration mode 1. Select Setup>Instrument 2. Select Simulator 3. Select

1.

Select Setup>Instrument

2.

Select Simulator

3.

Select File>Save

The features of ADAP Basic will be presented with an option to simulate random measurements

3. Select File>Save The features of ADAP Basic will be presented with an option to simulate
3. Select File>Save The features of ADAP Basic will be presented with an option to simulate

ADAP 2.0 Basic Front page

ADAP 2.0 Basic Front page Icon Function Save data Copy data Print data Setup a quick
ADAP 2.0 Basic Front page Icon Function Save data Copy data Print data Setup a quick

Icon

Function

Save data

Save data

Copy data

Copy data

Print data

Print data

Setup a quick

Setup a quick

measurement

Load plate

Load plate

Eject Plate

Eject Plate

Initiate reader

Initiate reader

Change users

Change users

Quick Measurements 1. Select quick measurement icon 2. Select Plate Type 3. Select Measurement Position
Quick Measurements 1. Select quick measurement icon 2. Select Plate Type 3. Select Measurement Position
Quick Measurements 1. Select quick measurement icon 2. Select Plate Type 3. Select Measurement Position

Quick Measurements

1. Select quick measurement icon

2. Select Plate Type

3. Select Measurement Position

4. Select Measurement Mode

a) Endpoint Photometric for single endpoint wavelength measurements (with or without a reference filter*)

b) Kinetic Photometric for a series of measurements over a specified length of time

c) Multiwavelength for one time measurement of more one wavelength

5. Select Start

over a specified length of time c) Multiwavelength for one time measurement of more one wavelength

Why use a reference filter/wavelength?

Why use a reference filter/wavelength?  Like a spectrophotometer it is important to use a reference

Like a spectrophotometer it is important to use a reference measurement.

Reference measurements can be a blank within the plate layout (which is

automatically subtracted from all the non-blank measurements on the

plate).

Eliminates interference from other absorbing substances

Or a reference wavelength/filter can be used. A wavelength is chosen where the sample of interest does not absorb. This measurement is automatically subtracted from the measurement wavelength/filter.

Eliminates interference from the plate, dust lint or other irregularities in the well.

Good practice is to use both a blank and a reference wavelength.

dust lint or other irregularities in the well.  Good practice is to use both a

Presentation of Results

Presentation of Results  OD represents absorbance measurements with reference measurements subtracted  Status
Presentation of Results  OD represents absorbance measurements with reference measurements subtracted  Status

OD represents absorbance measurements with reference measurements subtracted

Status represents instrument error that may have occurred during the measurements during the measurement of specific wells

Status represents instrument error that may have occurred during the measurements during the measurement of specific

Presentation of results

Presentation of results  Data can be copied and pasted into Excel using the Copy Icon:

Data can be copied and pasted into Excel using the Copy Icon:

Simply paste to transfer data to Excel or another program.

Data will paste as a matrix:

using the Copy Icon:  Simply paste to transfer data to Excel or another program. 
using the Copy Icon:  Simply paste to transfer data to Excel or another program. 
using the Copy Icon:  Simply paste to transfer data to Excel or another program. 

Presentation of results

Data can be printed using the print icon:

Presentation of results  Data can be printed using the print icon:
Presentation of results  Data can be printed using the print icon:
Presentation of results  Data can be printed using the print icon:

Presentation of Kinetic Data

Presentation of Kinetic Data  Data is presented as cycles equivalent to number of measurements 

Data is presented as cycles equivalent to number of measurements

Copy active window- copies only the shown matrix to the

clipboard

Copy copies all measurements

 Copy active window- copies only the shown matrix to the clipboard  Copy – copies
 Copy active window- copies only the shown matrix to the clipboard  Copy – copies

Presentation of Kinetic Data

Select

Presentation of Kinetic Data  Select and Copy in order to copy all kinetic data to

and Copy in

order to copy all kinetic data to the clipboard as

a list

Select

copy all kinetic data to the clipboard as a list  Select and Copy Active Window

and Copy

Active Window to copy only selected cycle to the clipboard as a matrix

to the clipboard as a list  Select and Copy Active Window to copy only selected
to the clipboard as a list  Select and Copy Active Window to copy only selected
to the clipboard as a list  Select and Copy Active Window to copy only selected
to the clipboard as a list  Select and Copy Active Window to copy only selected

Presentation of Kinetic Data

Presentation of Kinetic Data  Kinetic data is represented as a graph.  Clicking on a

Kinetic data is represented as a graph.

Clicking on a specific well represents a detailed graph.

Clicking on a specific well represents a detailed graph.  and Copy Active Window copies the

and Copy Active

Window copies the graph(s)

to the clipboard.

Clicking on a specific well represents a detailed graph.  and Copy Active Window copies the
Clicking on a specific well represents a detailed graph.  and Copy Active Window copies the
Clicking on a specific well represents a detailed graph.  and Copy Active Window copies the

Presentation of Multiwavelength Data

Presentation of Multiwavelength Data  Data is presented like kinetic data with an additional tab showing
Presentation of Multiwavelength Data  Data is presented like kinetic data with an additional tab showing
Presentation of Multiwavelength Data  Data is presented like kinetic data with an additional tab showing

Data is presented like kinetic data with an additional tab showing each well absorbance measurements in a table format.

Go to Raw Data tab and

Print Raw Data to see a

Data printout of all wavelength measurements

in a table format.  Go to Raw Data tab and Print Raw Data to see

Other features

Other features  View log table File> View Log Table shows who, what and when software

View log table File> View Log Table shows who, what and when software

was used.

table File> View Log Table shows who, what and when software was used.  Data can

Data can be saved or copied for printing

table File> View Log Table shows who, what and when software was used.  Data can

Other features

In Setup> Instrument there are two tabs that can be used to assess the performance of the instrument:

Function 1

Function 2

Note: Functionality of the setup functions is dependent

on the instrument in use.

 Function 1  Function 2 Note: Functionality of the setup functions is dependent on the
 Function 1  Function 2 Note: Functionality of the setup functions is dependent on the
 Function 1  Function 2 Note: Functionality of the setup functions is dependent on the

Other features

Other features  Go to Setup>System to customize data export and printouts
Other features  Go to Setup>System to customize data export and printouts

Go to Setup>System to customize data export and printouts

Other features  Go to Setup>System to customize data export and printouts

Summary: Features of ADAP 2.0 Basic

Summary: Features of ADAP 2.0 Basic  User and password controlled data with log file 

User and password controlled data with log file

Intuitive interface

Easy setup of quick measurements: endpoint, kinetic and multiwavelength

Simple export of data into Excel

Detailed, customizable printout

endpoint, kinetic and multiwavelength  Simple export of data into Excel  Detailed, customizable printout

ADAP 2.0 Levels are Accessed by License Codes

ADAP 2.0 Levels are Accessed by License Codes ADAP 2.0 License code Features Instrument versions

ADAP 2.0

License code

Features

Instrument

versions

Compatibility

Basic

No code required

Reader control

EZ Read 400

Zenyth 340/200 Anthos 2010/

2020

Plus

BRHPG-PIPEO-DOGNK-

Same as Basic AND:

EZ Read 400 Anthos 2010/

KEKDL-DSGKA

Quantitative analysis

(Using a standard curve)

2020

Zenyth 340

Qualitative analysis (using positive and negative controls)

Quality control

2020 Zenyth 340  Qualitative analysis (using positive and negative controls)  Quality control

Accessing the features of ADAP 2.0 Plus in

Demo Mode

Accessing the features of ADAP 2.0 Plus in Demo Mode  Select Help>Abou t to enter

Select Help>About to enter a demo

license code.

Enter in the following license code:

BRHPG-PIPEO-DOGNK-KEKDL-DSGKA

Select OK

Go to Setup> Instrument> Read Configuration

license code: BRHPG-PIPEO-DOGNK-KEKDL-DSGKA  Select OK  Go to Setup> Instrument> Read Configuration

Key Feature of ADAP 2.0 Plus

Test Definition (Analysis method)

Feature of ADAP 2.0 Plus Test Definition (Analysis method)  Configure plate layout  Quantitative with

Configure plate layout

Quantitative with essential curve-fitting

Qualitative with 5 categories

Transformation formulas

Replicate verification formulas

Test validation formulas

with 5 categories  Transformation formulas  Replicate verification formulas  Test validation formulas

ADAP 2.0 Plus Front Page

ADAP 2.0 Plus Front Page Icon Function Opens method setup Runs a single test using a
ADAP 2.0 Plus Front Page Icon Function Opens method setup Runs a single test using a
Icon Function Opens method setup Runs a single test using a previously defined method Recalculated
Icon
Function
Opens method setup
Runs a single test using a previously defined method
Recalculated results based on manual elimination or
restoration of wells.
single test using a previously defined method Recalculated results based on manual elimination or restoration of
Method Setup: General  Name test  Set measurement and reference filters  Set instrument

Method Setup: General

Method Setup: General  Name test  Set measurement and reference filters  Set instrument 

Name test

Set measurement and

reference filters

Set instrument

Define Plate Layout

Set lot# dependent

concentrations (e.g. antibody lots)

Set test specific parameters (e.g. calibration factors)

Set shaking

(e.g. antibody lots)  Set test specific parameters (e.g. calibration factors)  Set shaking (Zenyth 340

(Zenyth 340 only)

(e.g. antibody lots)  Set test specific parameters (e.g. calibration factors)  Set shaking (Zenyth 340

Plate Layout

Plate Layout  Place controls and samples in the plate  Use dilution factors  Preset
Plate Layout  Place controls and samples in the plate  Use dilution factors  Preset

Place controls and samples in the plate

Use dilution

factors

Preset replicates for easy plate filling

 Place controls and samples in the plate  Use dilution factors  Preset replicates for

Quantitative Analysis

Quantitative Analysis  Up to 10 standards can be defined  Curve-fitting with four options 

Up to 10 standards can be defined

Curve-fitting with four options

Linear or log axes

Curve validation

Used for many assay types including total protein and ELISAs.

for many assay types including total protein and ELISAs. Note : 4- parameter fit is a

Note : 4- parameter fit is a key curve-fitting algorithm for many ELISA assays and other competitive binding assays

Note : 4- parameter fit is a key curve-fitting algorithm for many ELISA assays and other

Quantitative Analysis- An example:

The Bradford Assay

Quantitative Analysis- An example: The Bradford Assay  The Bradford Assay is a total protein assay

The Bradford Assay is a total protein assay used to determine

the total amount of all protein in

a sample.

The Bradford assay uses known concentrations of bovine serum albumin (BSA) to draw a

standard curve by which to

calculate the concentrations of

samples.

concentrations of bovine serum albumin (BSA) to draw a standard curve by which to calculate the
concentrations of bovine serum albumin (BSA) to draw a standard curve by which to calculate the

Quantitative Analysis- An example:

The Bradford Assay

Quantitative Analysis- An example: The Bradford Assay  The concentration of standards are suggested by the

The concentration of standards are suggested by the assay kit manufacturer.

Linear regression is used to

draw a straight line

through the concentrations so that the concentrations of the samples can be determined.

Linear x and y axes will be

used to draw the graph

A minimum correlation or r2 value is set at >0.98

 Linear x and y axes will be used to draw the graph  A minimum
 Linear x and y axes will be used to draw the graph  A minimum

Results Presentation

Data is presented in multiple tabs.

Data can be copied to the

clipboard for import into another

software program, printed or saved.

in multiple tabs.  Data can be copied to the clipboard for import into another software
in multiple tabs.  Data can be copied to the clipboard for import into another software
in multiple tabs.  Data can be copied to the clipboard for import into another software

Qualitative Analysis

Qualitative Analysis  Define up to 5 categories  A transformation formula can be applied to

Define up to 5 categories

A transformation formula can be applied to the sample absorbances

Interpretation can be based on absorbance (OD), concentration or the transformed value.

This analysis is used mostly with

ELISA assays

value.  This analysis is used mostly with ELISA assays Note: Group 1 has the highest

Note: Group 1 has the highest absorbance values. Cutoff Formula 1 assumes a ‘>‘ in the

formula so that absorbance values of samples in Group 1>Group 2>Group 3 groups distinguished must be defined in the formula box.

How the

Qualitative Analysis-An example HTLV I and II

ELISA

Qualitative Analysis-An example HTLV I and II ELISA  Qualitative analysis of an ELISA for the
Qualitative Analysis-An example HTLV I and II ELISA  Qualitative analysis of an ELISA for the

Qualitative analysis

of an ELISA for the presence of HTLV infection

Plate layout specified

by assay kit

manufacturer

Assay measured at 450 nm. A reference measurement is also used at 620 nm

layout specified by assay kit manufacturer  Assay measured at 450 nm. A reference measurement is

Qualitative Analysis-An example HTLV I and II

ELISA

Qualitative Analysis-An example HTLV I and II ELISA  Analysis is specified by the manufacturer 

Analysis is specified

by the manufacturer

All absorbance measurements (X) are compared to the negative control

using this formula:

Index = Sample (OD)/

NC1(OD)+0.45

The index value is used to categorize the samples.

control using this formula: Index = Sample (OD)/ NC1(OD)+0.45  The index value is used to
control using this formula: Index = Sample (OD)/ NC1(OD)+0.45  The index value is used to

Qualitative Analysis-An example HTLV I and II

ELISA

Qualitative Analysis-An example HTLV I and II ELISA Replicate Rejection:  Avg PC1-NC1>=0.800, 1 replicate 

Replicate Rejection:

Avg PC1-NC1>=0.800, 1 replicate

PC1>=0.800, 2 replicates

Validation Criteria:

NC1<= 0.100

Each PC1 must be within 30% of

the average of PC1

Blank <= 0.100 OD

Criteria:  NC1<= 0.100  Each PC1 must be within 30% of the average of PC1
Criteria:  NC1<= 0.100  Each PC1 must be within 30% of the average of PC1

Define Results Presentation

Define Results Presentation  Determine how mean values will be calculated  Determine how the Blank

Determine how mean values will be calculated

Determine how the Blank will be used

Configure print options

Select evaluation method for controls

 Determine how the Blank will be used  Configure print options  Select evaluation method
 Determine how the Blank will be used  Configure print options  Select evaluation method

Results Presentation

Results are presented on multiple tabs

Info Calculation and Results List show the results

Results Presentation  Results are presented on multiple tabs  Info Calculation and Results List show
Results Presentation  Results are presented on multiple tabs  Info Calculation and Results List show
Results Presentation  Results are presented on multiple tabs  Info Calculation and Results List show

Results Presentation

Results list

Interpretation

Test Validation

Replicate Rejection

Results Presentation Results list  Interpretation  Test Validation  Replicate Rejection
Results Presentation Results list  Interpretation  Test Validation  Replicate Rejection
Results Presentation Results list  Interpretation  Test Validation  Replicate Rejection

Results Presentation

Results Presentation  Select >Copy  All tabs are copied into the clipboard  Select >Copy

Select >Copy

All tabs are copied into the clipboard

Select

 All tabs are copied into the clipboard  Select >Copy Active Window to copy data

>Copy Active Window

to copy data from specific data to the clipboard

tabs are copied into the clipboard  Select >Copy Active Window to copy data from specific
tabs are copied into the clipboard  Select >Copy Active Window to copy data from specific

Results Presentation

Results Presentation  Select  Printout configuration is a result of parameters set in Setup>System and

Select

Printout configuration is a

result of parameters set in

Setup>System and Test Definition

Presentation  Select  Printout configuration is a result of parameters set in Setup>System and Test
Presentation  Select  Printout configuration is a result of parameters set in Setup>System and Test
Presentation  Select  Printout configuration is a result of parameters set in Setup>System and Test

Recalculate Options

Right click on the results and select Formula. Cutoff and test validation formulas can be changed.

Specific wells can be deleted or restored; data can then be recalculated

by selecting

formulas can be changed.  Specific wells can be deleted or restored; data can then be
formulas can be changed.  Specific wells can be deleted or restored; data can then be
formulas can be changed.  Specific wells can be deleted or restored; data can then be
formulas can be changed.  Specific wells can be deleted or restored; data can then be
formulas can be changed.  Specific wells can be deleted or restored; data can then be
formulas can be changed.  Specific wells can be deleted or restored; data can then be

Running a test definition

Running a test definition  Select  A dialogue window will appear, select test to run

Select

A dialogue window will appear, select test to run and OK

A window will open to prompt for the number of samples. The sample number defined in the test definition will appear. Select OK or change to new sample number.

number of samples. The sample number defined in the test definition will appear. Select OK or
number of samples. The sample number defined in the test definition will appear. Select OK or
number of samples. The sample number defined in the test definition will appear. Select OK or

Key Features of ADAP 2.0 Plus

Key Features of ADAP 2.0 Plus  Quantitative and qualitative analysis- for most assays  Quality

Quantitative and qualitative analysis- for most assays

Quality controls for replicate rejections and test validation

Recalculation Options

Options for printouts and data export

Quick method setup

and test validation  Recalculation Options  Options for printouts and data export  Quick method

Questions and Feedback

Questions and Feedback  More advanced analysis options are available for the Zenyth 340 and Zenyth

More advanced analysis options are available for the Zenyth 340 and Zenyth 200 microplate readers. Requirement for an advanced ADAP 2.0 webinar?

Questions from the field?

Customer perception of ADAP 2.0?

Other suggestions for upcoming webinars?

 Questions from the field?  Customer perception of ADAP 2.0?  Other suggestions for upcoming