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10. Anchor the vein by holding the patient’s arm and placing a thumb BELOW the place
where you want to place the needle.
DO NOT touch the disinfected site. DO NOT place a finger over the vein to guide
the needle.
11. Perform the blood draw.
Enter the vein swiftly at a 30 degree angle.
Line up the needle with the vein. Make sure the bevel is up
12. When blood starts to flow, ask patient to open his/her hand
13. Once sufficient blood has been collected (minimum 5ml), release the
tourniquet BEFORE withdrawing the needle
14. Withdraw the needle gently.
Give the patient a clean gauze or dry cotton wool ball to press gently on the site.
Ask the patient NOT to bend the arm.
15. Remove blood collector tube from holder and put into rack.
16. Put needle into leak-proof and puncture resistant sharps container.
17. Stop the bleeding and clean the skin.
Do not leave patient until bleeding has stopped.
Put an adhesive bandage on the site, if necessary.
18. Put items that drip blood or have body fluids on them into the infectious waste
bag.
19. Ask the designated assistant to approach the patient room, without entering.
This person should have gloves on. This person should come close to you holding the
open plastic leak-proof packaging container.
20. The person who has collected the blood sample should put the wrapped tube of
blood into the plastic leak-proof packaging container for transport to the
respective laboratory.
Be careful not to touch outside of leak-proof plastic tube with gloves.
21. Remove Personal Protective Equipment (PPE) and do Handwashing.
IV Cannulation and Infusion
Pre-Catheterization or Preparation
Wash hands with either a non-antimicrobial soap and water or an antimicrobial soap
and water when hands are visibly dirty or contaminated with blood or other body fluids.
If hands are not visibly soiled, use an alcohol-based hand rub to avoid routinely
contaminating hands in all other clinical situations.
Decontaminate hands before having direct contact with patients
Do not wear artificial fingernails or extenders when having direct contact with patients
at high risk
3. Gather Equipment
Prepare and gather the equipment’s needed for starting the IV. Always check for the
fluid’s expiration date.
Inspect solution container for integrity.
Glass containers. Hold up to light to look for cracks, clarity, particulate contamination,
and expiration date.
Plastic containers. Squeeze to check for pinholes, clarity, particulate contamination, and
expiration date.
Inspect administration set
Choose the appropriate set: vented or nonvented
Gather venipuncture and dressing supplies
Catheter (22 g, 20 g most common)
Dressing (gauze)
Tape: 1-inch paper
Prepping solution
Gloves 2×2 gauze
4. Patient Assessment and Psychological Preparation
It’s important to also prepare the patient on the procedure.
Introduce self and verify the client’s identity.
Provide privacy
Explain the procedure to the client. A venipuncture can cause discomfort for a few
seconds, but there should be no discomforts while the solution is flowing.
Evaluate the patient preparedness for IV procedure by talking with patient before
assessing veins
There are several factors you need to consider before initiating venipunctures:
Tourniquet. Latex or non-latex used most frequently. Placed 6–8 inches above the
venipuncture site. If BP high, move farther from venipuncture site. If BP low, move as
close as possible without risking site contamination.
Gravity. Position the extremity lower than the heart.
Fist clenching. Instruct patient to open and close his/her fist.
Tapping vein. Using thumb and second finger, flick the vein; this releases histamines
beneath the skin and causes dilation. Do not slap the vein.
Warm compresses. 10 minutes maximum. Do not use microwave!
Blood pressure cuff. Inflate to 30 mmHg; great for fragile veins.
Multiple tourniquet technique. Use 2 to 3 latex tourniquets; apply one high on arm and
leave for 2 minutes; apply second at mid arm below antecubital fossa; collateral veins
should appear; apply third if needed.
Suitable vein should feel relatively smooth and pliable, with valves well-spaced.
Start with distal veins and work proximally.
Veins that feel bumpy (like running your finger over a cat’s tail) are usually thrombosed
or extremely valvular. Veins will be difficult to stabilize in a patient who has recently lost
weight.
Catheterization or Catheter Insertion
6. Needle Selection
The smaller the gauge number, the thicker the catheter and the more rapidly medicine
can be administered and blood can be drawn.
Thicker catheters cause more painful insertion, so it’s very necessary not to use a
catheter that’s larger than you need.
The tip of the catheter should be inspected for integrity prior to venipuncture.
Only two attempts at venipuncture are recommended.
20G Pink Multi-purpose IV; for medications, hydration, and routine therapies.
22G Blue Most chemo infusions; patients with small veins; elderly or pediatric patients
Post-Catheterization