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Instructions
Submit via Moodle.
Your answers should be saved as a PDF. It is your own responsibility to ensure
that all text and images are present and correct when saving. Check your file
before submitting it. Ensure your answers are labelled with the question number
so they can be efficiently marked.
There is no need to include software output (Excel or SPSS) other than graphs
and charts where are asked for. Relevant output from the software should be
included as specified in how to report your results from the course notes. Your
Excel spreadsheets and SPSS output will not be collected or marked.
When any graphical output is asked for, ensure the axis are labelled, and the
chart given a title and/or caption. Use legends where appropriate.
Carry forward exact results in your analysis, but give numerical answers to a
sensible level of accuracy (decimal places). Beware illusory accuracy.
Problem 1 (29 Marks)
A recently developed antibiotic (with unknown kinetics) was given to a group of
patients (a total of 10 men aged between 25 and 35 years) by IV bolus injection
(100 mg of drug injected directly into the systemic circulation – a loading dose).
To measure how the antibiotic concentration changed with time, blood samples
were taken at regular intervals. The spreadsheet marked ‘drug decay’ shows the
mean concentration of the antibiotic in the blood, and the standard deviation of
these measurements.
a) What are standard deviations a measure of?
(1 mark)
b) Create a scatter plot for the concentration of antibiotic in the blood
samples against time, with the standard deviations in concentration set as
error bars. Why do you think the concentration of the antibiotic falls with
time? Find the Pearson correlation coefficient; determine how correlated
drug concentration is with time.
(6 marks)
c) What is the difference between zero order and first order decay?
(2 marks)
d) By visual examination of your graph and the data, estimate the half-life of
the drug.
(1 mark)
e) Despite drugs usually exhibiting first order decay, these data appear to
follow zero order decay. Use the ‘add trendline’ function (including
equation display) to fit a straight-line trendline to the scatter plot. The
negative of the slope of the line is equal to the rate constant of elimination
(Kel). What is the value of Kel and what are its units?
(2 marks)
f) Zero order decay can be described using equation 1.
𝐶" = 𝐶0 − 𝐾𝑒𝑙×𝑡 (1)