12/12/2017 Comparison of TAE and TBE electrophoresis buffer ­ Lab Protocols

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Lab Protocols > Methods > Electrophoresis > Gel Electrophoresis > Comparison of TAE and TBE electrophoresis
bu er
APR 1 Comparison of TAE and TBE electrophoresis
2017
bu er
Properties TBE TAE Remarks

Bu ering capacity High Low TAE become exhausted during extended or

repeated electrophoresis

Migration of (double Slow Fast TAE has better conductivity

stranded) DNA

Resolution High resolution High resolution for TBE supports agarose cross-linking better than

long DNA Short DNA TAE

fragments fragments

Enzymatic Poor Good Borate from TBE bu er is an inhibitor of many

modi⒄�cation of gel commonly used enzymes in molecular biology

puri⒄�ed DNA

Recovery of DNA Poor Good Borate from TBE bu er interacts with DNA
from agarose gel

Integrity of DNA High Low Borate from TBE bu er inhibits many enzyme
activity including DNA modifying enzymes

Cost Costly Cheaper Highly conc. TAE (50X) require less volume to
be transported.

Active bu er Tris – Acetate Tris – Borate

Working conc for 1X 0.5X

DNA electrophoresis

 
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