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Experiment No. 2
Article history: The protein, Casein, was isolated from the non-fat milk using
Date Performed: December 6, 2017 isoelectric precipitation with acetic acid. Subsequent to the isolation, the
Date Submitted: December 13, 2017 protein underwent acid and alkaline hydrolysis. The intact protein and
both acid and basic hydrolyzates were subjected to various qualitative
color reactions namely, Biuret test, Ninhydrin test, Xanthoproteic test,
Millon’s test, Hopkins-Cole test, Sakaguchi test, and Fohl’s test. The
Keywords:
intact protein and both of the hydrolyzates were analyzed using
Isoelectric precipitation qualitative color reactions. All samples gave positive results for Biuret's,
Casein Ninhydrin, Millon's and Sakaguchi tests. All of samples gave negative
Qualitative color reactions result on Xanthoproteic test. And lastly both hydrolyzates gave negative
results to Hopkins-cole and Fohl's tests but the intact protein gave
positive results.