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- branch of Biology which deals with the study of minute organisms not visible to the
naked eye.
SCOPE :
1. BACTERIOLOGY – Bacteria
2. MYCOLOGY – Fungi
3. PARASITOLOGY – Protozoa & Parasitic worms
4. PHYCOLOGY – Algae
5. VIROLOGY – Viruses
USES OF MICROBES :
MICROSCOPY
I. Brightfield Microscopy
- objects are observed against a bright background
- inexpensive and easy to use
- used to observe various stained specimens and to count microbes
II. Darkfield Microscopy
- uses a special condenser with an opaque disc that blocks light from entering the
specimen
-specimen appears light against a dark background
- used to examine living microorganisms that are invisible in brightfield
microscopy, do not stain easily or are distorted by staining
- used to detect T.pallidum in the diagnosis of syphilis