<Contact>

ADEKA Corporation New Business Promotion Department

〒116-8553 7-2-35 Higashi-Ogu, Arakawa-ku, Tokyo JAPAN
Revised 2009.3.19
TEL +81-3-4455-2829 FAX +81-3-3809-8223

Fermented Beta-Glucan (LQ/Powder)

CH2OH
O
HO O
HO
OH
CH2
CH2OH O
O
HO HO
O O O
OH OH
m n

(C6H10O5)n

Fermented Beta-Glucan has high water Retentivity, as well as many other useful features. It is
known as a functional component of mushrooms and yeasts, and is widely applied in pharmaceuticals
and food ingredients.

Manufacturing Method
Fermented Beta-Glucan LQ (FBG) is produced by extraction of extracellular β-Glucan produced by the
black yeast Aureobasidium pullulans. It’s major component is β-D-1,3-1,6-Glucan. (The product is
available in liquid form (LQ, 1% Beta-Glucan) and in powder form (85% Beta-Glucan).

Product Specification
Fermented Beta-Glucan
Product Name Fermented Beta-Glucan LQ
Powder
White to pale yellow powder with a
Appearance Clear to white, viscous liquid
slight odor

β-1,3-1,6-Glucan > 1% > 85%

(1) Absorbance at 3360 ~ 3320cm , 2900 ~ 2880cm-1, 1375 ~

-1

1365cm-1, 1040 ~ 1030 cm-1, 890cm-1 by IR spectrometry (ATR Method)

Identification Test
(2) Addition of 20 mL ethanol to 10 mL of liquid product or of 1% powder

product solution forms gel precipitation or white precipitation.

Heavy Metal （as Pb） < 20ppm < 20ppm

Purity Test
Arsenic （as As2O3） < 2ppm < 2ppm

SPC < 30 CFU/g < 1×103 CFU/g

Biology Test
Coliforms Negative Negative

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Storage and Handling
Fermented Beta-Glucan LQ
Store at 3 ~ 15 oC. No additives/preservatives are used.
Please use as soon as possible once opened.
Fermented Beta-Glucan Powder
Avoid high temperature and high humidity, and store below 25 oC.
Please use as soon as possible once opened, if any product remains, seal the cap tightly.

Ingredients
Sucrose, Yeast Extract, Minerals, Aureobasidium pullulans

Application
Can be applied as a humectant in cosmetics (basic skin care products, hair care products,
sunscreens).

Physical Properties
Viscosity

1000 <Transparency>
Viscosity (mPa ･s)

100

10

1
From left to right: Water, 0.5%, 2% Solution
0 0.5 1 1.5 2 2.5
β-Glucan Concentration (%)

Measurement: E-type Viscometer (VISCOMETER TV-20 TOKIMEC), 22 oC

Solution Transparency

Concentration (%) Abs. (660nm)

0.10 0.001 Notes: Solution with 1% or more Beta-Glucan

0.25 0.004 concentration may whiten but whiteness

0.50 0.005 will clear by heating (above 35 oC).

1.0 0.013

2.0 0.028

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Water Retentivity

Water Retentivity Comparison （2hr Retention Time）

100 600

Water Retentivity

Solution Viscosity (mPa ・s)

500
80 +G Water Retentivity
Water Retentivity (%)

Viscosity
400
+G Viscosity
60
+G: 5% Glycerin added
300
40
200

20
100

0 0
1 2 3 4

1 Water -
2 Fermented Beta-Glucan MW 300 kD
3 Sodium Hyaluronate （HANa） MW 300 kD
4 Sodium Hyaluronate （HANa） MW 800 – 1170 kD
Sample: 1mL of 2% solution (Each powder was dissolved in water, heated for 10min at 70oC,
then left still for 30min, and poured onto filter paper)
Water Retention after 2hr Evaporation (1atm, 22oC, 50% Humidity)
(Moisture before Evaporation - Evaporated Moisture) * 100
Water Retention (%) =
Moisture before Evaporation

Water Retentivity Changes

Water Retentivity of Fermented Beta-Glucan (FBG) Viscosity and Water Retentivity Relation (2% Solution)
100 100
Water FBG
5% Glycerin
Hyaluronate Na MW:300kD
0.5% FBG
80 1.0% FBG 80 Hyaluronate Na MW:800-1170kD
Water Retentivity (%)

Water Retentivity (%)

2.0% FBG
2% FBG + 5% Glycerin
60 60

40 40

20 20

0 0
0 0.5 1 1.5 2 2.5 3 3.5 0 100 200 300 400 500
Retention Time (hr) Viscosity (mPa･s)

Time Course of Water Retentivity Time Course of Water Retentivity (5% Glycerin added)

100 100
Water Water
2% FBG 5% Glycerin
80 2% HANa MW:300kD 80 2% FBG +G
Water Retentivity (%)

Water Retentivity (%)

2% HANa MW:800-1170kD 2% HANa MW:300kD +G

2% HANa MW:800-1170kD +G
60 60

40 40

20 20

0
0
0 0.5 1 1.5 2 2.5 3 3.5
0 0.5 1 1.5 2 2.5 3 3.5
Retention Time (hr)
Retention Time (hr)

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Stability Test
FBG: Fermented Beta-Glucan, HANa: Sodium Hyaluronate, G: Glycerin

Concentration (w/w)
100%
80%

β-Glucan
60%
40%
25℃ 4℃
20%
0%
0 2 4 6 8 10 12
Storage (months)

Safety Tests

Test Result

Single Dose Oral Toxicity (Rat) LD50: above 2,000 mg/kg

Primary Skin Irritation Test (Rabbit) Negative

Repeated Skin Irritancy Test (Rabbit) Negative

Skin Sensitization Test (guinea pig) Negative

Phototoxicity Test No maximum absorption

Contact Photosensitization Test observed at 290 - 400nm

Eye Irritancy Test (Rabbit) Negative

Ames Test Negative

Genotoxicity Chromosomal Aberration Test Negative

Micronucleus Test (Mouse) Negative

Closed Patch Test (Human) Negative

INCI Labeling

Product Name INCI Name

Fermented Beta-Glucan LQ AUREOBASIDIUM PULLULANS FERMENT

Fermented Beta-Glucan Powder BETA-GLUCAN

Package
Fermented Beta-Glucan LQ 10 kg (Bag contained in a box)
Fermented Beta-Glucan Powder 100g or 500g bottle

For further information and sample requests, please contact:

ADEKA Corporation New Business Department TEL: 03-4455-2829 FAX: 03-3809-8223

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