TASK BLENDED LEARNING 13.5.

2016, FRIDAY 3-5PM

A. The MAG synthesis using fish oil is important to be constructed in correct steps as the esterification
of MAG is sometimes presented in non-established steps that shall ruining the products. List out the
methods construct for the synthesis.

1. Esterification was conducted in a 50 mL vessel stirring at 500 rpm using a magnetic stirrer.

2. The reaction mixture was composed of fatty acid, glycerol, and lipase solution.

3. The various lipases, reaction temperature, ratio of fatty acid to glycerol, amounts of lipase, and
the organic solvent, were changed to study their effects on esterification.

4. The MAG selectivity was expressed as the percentage of MAGs to total acylglycerols (MAGs +
DAGs + TAGs) in the reaction mixture.

5. The experiment was performed in triplicate. Values are expressed as means ± standard
deviations

B. Classify details about upstream processing that may be appearing in the bioprocess industry

1. The process of growing microorganisms (microbes) to produce end products →Fermentation

2. Includes formulation of the fermentation medium, sterilization, fermentation medium, inoculum

preparation and build up, and inoculation of the fermentation medium

3. Sterilizations essential for preventing the contamination with any undesired microorganisms.

4. Microbes are usually incubated under specific conditions in large tanks called fermenters or
bioreactors.

5. There many different types of fermenter used such as STR, Air-Lift, membrane bioreactor

6. Fermenters vary in size from 5 to 500,000 liters •Large-scale fermenters are almost always stainless
steel