Microbiological Sampling Plans: A Tool To Explore ICMSF Recommendations

Microbiological sampling plans: a tool to explore ICMSF recommendations
Version 2.05: Copyright 1998 David Legan & Mark Vandeven 2009 Marcel Zwietering & Peter Sestoft
Disclaimer
This spreadsheet calculates probabilities The authors have taken every care to ensure that the output from this
of acceptance for materials with different workbook is accurate. In making this tool available to promote discussion
microbial loads and population standard and understanding of the power and limitations of attributes sampling plans
deviations. The microbes are assumed neither the authors nor Nabisco Inc / ICMSF accept any liability for any
to be lognormally distributed. consequences, direct or indirect resulting from a decision by the user to take,
or not to take, any action based on an output from this workbook.
Version 1.01 Version 2.01: Addition of enrichment calculations

Mark Vandeven and David Legan Marcel Zwietering
Nabisco Research Wageningen University
R M Schaeberle Technology Center Laboratory of Food Microbiology
200 DeForest Avenue PO Box 8129
East Hanover, NJ 07936-1944, USA 6700EV Wageningen
email: VandevenM@Nabisco.com, or The Netherlands
LeganD@Nabisco.com email: Marcel.Zwietering@WUR.nl
Background reading
ICMSF book 2
Calculations for 2-class spreadsheet were checked against ICMSF book 2 Tables 2 and 3 and are in complete agreement.
Calculations for 3-class spreadsheet were checked against ICMSF book 2 Table 4 and are in complete agreement.
Relating microbiological criteria to food safety objectives and performance objectives
M. van Schothorst, M.H. Zwietering, T. Ross, R.L. Buchanan, M.B. Cole, International Commission on Microbiological Specifica
Food Control 20: 967-979 (2009)
Acknowledgment
The curves showing the distribution of organisms in foods for entered mean counts and sigma values
were inspired by unpublished work circulated to ICMSF members by Suzanne Dahms
Development of this tool was inspired by Martin Cole
update 2009-05-20
update 2009-10-7: reduced further in calculations, removed empty modules, updated MZ
update 2009-10-12: returned to goalseek, no need for solver and analytic toolpack
update 2009-10-14: limited c maximally at 1e10 cfu/g in poissonlognormal
update 2009-10-15: limited sigma >0.05
update 2009-10-27: protected help file in Introduction
utput from this
romote discussion
utes sampling plans
y liability for any
on by the user to take,
Version 2.02: Simpliciation and reorganisation

This version simplified and reorganized by Peter Sestoft (sestoft@itu.dk) after discussions
with Marcel Zwietering (marcel.zwietering@wur.nl)
Version 2.03-2.05: Finilisation for posting

The file is further improved and updated.
complete agreement.
te agreement.
n Microbiological Specifications for Foods (ICMSF)

Working with the ICMSF sampling tool
Downloading the spreadsheet and technical issues

1) Download the ICMSF sampling tool by opening the web page:
http://www.icmsf.iit.edu/main/home.html
2) Select Download Standard Program in the right part of the screen, labelled as
Download Standard Program (sampleplans2_05.xls, 0.3M)
3) Respond to the question open or save the file by choosing save and save the file in an appropriate location.
4) Open this Excel file (sampleplans2_05.xls)
5) If you get a security warning concerning macros, please press enable macros. (The macros are not viruses: instead they add useful functions to the
spreadsheet).
For excel 2007 you have to enable macros in boxes top left of the screen
6) Select the tab 2class enrichment
7) As opened cell M19 in the spreadsheet most probably reads 5%. Change cell J19 to -2. Cell M19 is now automatically recalculated and for this
reason is no longer 5%. Click on the button Find mean that gives in the grey box below cell M20. If you do not get a security warning and if Cell
M19 changes to 5%, the file is working correctly and you can go on at the examples in the Introduction (other worksheet).
If you do get a security warning, or if cell M19 does not change to 5%, your system is not yet working correctly with the software and you should
consider the following:
8) FOR PC/Windows USERS:

If macros are not enabled:
Go to the Tools pull-down menu, select macros security and set security level at medium, read the file again and continue at 5, now it should
work.
9) FOR Macintosh USERS:
For Excel users, the macros (linked to buttons) will probably not work as intended. Users of Excel 2004 for Macintosh may find that the buttons
work, but they will probably not operate as intended in Excel X or Excel 2008. Other functions will operate normally, however, and many calculations
can still be performed.
For Users with recent Macintosh computers
If you want to use the full capability of the spreadsheet, it will be better if you can use Excel 2004 (part of Mac for Office, 2004), or set your computer up to
For Excel users, the macros (linked to buttons) will probably not work as intended. Users of Excel 2004 for Macintosh may find that the buttons
work, but they will probably not operate as intended in Excel X or Excel 2008. Other functions will operate normally, however, and many calculations
can still be performed.
For Users with recent Macintosh computers
If you want to use the full capability of the spreadsheet, it will be better if you can use Excel 2004 (part of Mac for Office, 2004), or set your computer up to
Description of the used variables
Data entry boxes are highlighted in yellow: All other cells are protected.
mean mean of the (assumed) log normal distribution describing the occurrence of bacterial contaminants; unit is log cfu p
sigma standard deviation of the same (assumed) log normal distribution
m acceptable level of microbiological contamination, defined as an allowable concentration, or for enrichement no con
n number of samples tested
c number of samples whose contamination is allowed to exceed m (that is, test positive for contamination), yet the lo
amount sample weight, in gram
P(accept) the probability of accepting a specific lot; this is a function of the assumed contamination level (mean, sigma) and t
For a manual please open the following word-file

aminants; unit is log cfu per gram
or for enrichement no contamination in a sample of a certain weight, hence equals minus log(weight)
contamination), yet the lot will be accepted
evel (mean, sigma) and the sampling plan (n, c and amount)
Operating characteristic curve for proportion defective, Probability density function (PDF) for
with n=10 and c=0 Distribution mean = -2.25 and sigm
1.0000 0.6
0.5
0.8000
0.4
0.6000
P(accept)
0.3
P
0.4000 1 0.2
0.1
0.2000
0
0.0000 -6 -5 -4 -3 -2 -1
0.0000 0.2000 0.4000 0.6000 0.8000 1.0000
Log count/g
proportion
PacceptdefectivePd
PDF m
INPUTS
Batch acceptance for Pd mean -2.25
P(accept) sigma 0.80
Pd 20 % 10.7 % m -1.40
actualPd #VALUE! % #VALUE! % n 10
c 0
amount 25 g
Sandbox: for your own calculations
Means and median

Arithmetic
0.0307 cfu/g
one cfu in 32.6 grams
The calculations below are for the buttons above. Do not edit.
mean pa pd P(accept)
-2.2 #VALUE! #VALUE! #VALUE! <= Used by macros for finding mean and n
0.8000 0.2000 0.1074 <= Used in test against ICMSF tables
-2.2 #VALUE! #VALUE! #VALUE! <= Used by macro for finding m in equivalent performa
The calculations below are for the charts above. Do not edit.
-6.0 #VALUE! #VALUE! #VALUE! 0.0000
-4.8 #VALUE! #VALUE! #VALUE! 0.0031 Coordinates to place m
-4.7 #VALUE! #VALUE! #VALUE! 0.0046 proportion m
-4.6 #VALUE! #VALUE! #VALUE! 0.0067 0 0 -1.39794
-4.5 #VALUE! #VALUE! #VALUE! 0.0095 0.5485 1 -1.39794
-3.8 #VALUE! #VALUE! #VALUE! 0.0763 Coordinates to place Pd
-3.7 #VALUE! #VALUE! #VALUE! 0.0964 p accept
-3.6 #VALUE! #VALUE! #VALUE! 0.1200 x y
-3.5 #VALUE! #VALUE! #VALUE! 0.1470 #VALUE! 0.0000
-3.4 #VALUE! #VALUE! #VALUE! 0.1774 #VALUE! 0.0500
0.0 #VALUE! #VALUE! #VALUE! 0.0096
nsity function (PDF) for log counts.
mean = -2.25 and sigma = 0.80
0.8
0.6
P
0.4
0.2
-4 -3 -2 -1 0 1 2 0
-6 -5 -4 -3 -2 -1 0 1 2
Log count/g
Mean log count/g
PDF m Prob. acceptance m
P(accept)
Computed #VALUE! %
Desired 5 %
Means and median

Geometric=median
0.0056 cfu/g
one cfu in 177.7 grams
ding mean and n
ing m in equivalent performance criterion

Operating characteristic curve scaled to relate mean log
count to m
0.8
0.6
P
0.4
0.2
0
-6 -5 -4 -3 -2 -1 0 1 2
Mean log count/g
Prob. acceptance m
ALTERNATIVE n AND c P(accept)

mean -2.25 Computed #VALUE! %
sigma 0.80 Target, left #VALUE! %
m -0.98
n 30
c 0
amount 9.6 g
Implied Acceptance level

Percentile z-score Concentration at this percentile
99.9 3.10 0.23
Operating characteristic curve for proportion defective, Probability density function (PDF) for
with n=10 and c=0 Distribution mean = 1.48 and sigm
1.0000 0.6
0.5
0.8000
0.4
0.6000
P(accept)
0.3
P
0.4000 1 0.2
0.1
0.2000
0
0.0000 -5 -4 -3 -2 -1 0 1 2
0.0000 0.2000 0.4000 0.6000 0.8000 1.0000
Log count/g
proportion
PacceptdefectivePd
PDF m
INPUTS
Batch acceptance for Pd mean 1.48
P(accept) sigma 0.80
Pd 10 % 34.9 % m 2
actualPd 25.9 % 5.00 % n 10
c 0
Means and median

Arithmetic
165.7 cfu/g
mean pa pd P(accept)
1.5 0.7411 0.2589 0.0500 <= Used by macros for finding mean and n
0.9000 0.1000 0.3487 <= Used in test against ICMSF tables
1.5 0.9050 0.0950 0.0500 <= Used by macro for finding m in equivalent performa
-4.0 1.0000 0.0000 1.0000 0.0000
-3.9 1.0000 0.0000 1.0000 0.0000
-3.8 1.0000 0.0000 1.0000 0.0000
-3.7 1.0000 0.0000 1.0000 0.0000
-3.6 1.0000 0.0000 1.0000 0.0000
-3.5 1.0000 0.0000 1.0000 0.0000
-3.4 1.0000 0.0000 1.0000 0.0000
-3.3 1.0000 0.0000 1.0000 0.0000
-3.2 1.0000 0.0000 1.0000 0.0000
-3.1 1.0000 0.0000 1.0000 0.0000
-3.0 1.0000 0.0000 1.0000 0.0000
-2.9 1.0000 0.0000 1.0000 0.0000
-2.8 1.0000 0.0000 1.0000 0.0000 Coordinates to place m
-2.7 1.0000 0.0000 1.0000 0.0000 proportion m
-2.6 1.0000 0.0000 1.0000 0.0000 0 0 2
-2.5 1.0000 0.0000 1.0000 0.0000 0.5485 1 2
-2.4 1.0000 0.0000 1.0000 0.0000
-2.3 1.0000 0.0000 1.0000 0.0000
-2.2 1.0000 0.0000 1.0000 0.0000
-2.1 1.0000 0.0000 1.0000 0.0000
-2.0 1.0000 0.0000 1.0000 0.0000
-1.9 1.0000 0.0000 1.0000 0.0001
-1.8 1.0000 0.0000 1.0000 0.0001 Coordinates to place Pd
-1.7 1.0000 0.0000 1.0000 0.0002 p accept
-1.6 1.0000 0.0000 1.0000 0.0003 x y
-1.5 1.0000 0.0000 0.9999 0.0005 0.2589 0.0000
-1.4 1.0000 0.0000 0.9999 0.0008 0.2589 0.0500
-1.3 1.0000 0.0000 0.9998 0.0012
-1.2 1.0000 0.0000 0.9997 0.0018
-1.1 0.9999 0.0001 0.9995 0.0027
-1.0 0.9999 0.0001 0.9991 0.0040
-0.9 0.9999 0.0001 0.9986 0.0059
-0.8 0.9998 0.0002 0.9977 0.0085
-0.7 0.9996 0.0004 0.9963 0.0121
-0.6 0.9994 0.0006 0.9942 0.0168
-0.5 0.9991 0.0009 0.9911 0.0231
-0.4 0.9987 0.0013 0.9866 0.0313
-0.3 0.9980 0.0020 0.9800 0.0417
-0.2 0.9970 0.0030 0.9706 0.0546
-0.1 0.9957 0.0043 0.9575 0.0705
0.0 0.9938 0.0062 0.9396 0.0896
0.1 0.9912 0.0088 0.9156 0.1120
0.2 0.9878 0.0122 0.8843 0.1380
0.3 0.9832 0.0168 0.8442 0.1673
0.4 0.9772 0.0228 0.7944 0.1996
0.5 0.9696 0.0304 0.7344 0.2346
0.6 0.9599 0.0401 0.6644 0.2714
0.7 0.9479 0.0521 0.5857 0.3091
0.8 0.9332 0.0668 0.5009 0.3465
0.9 0.9154 0.0846 0.4133 0.3825
1.0 0.8944 0.1056 0.3274 0.4157
1.1 0.8697 0.1303 0.2476 0.4448
1.2 0.8413 0.1587 0.1777 0.4685
1.3 0.8092 0.1908 0.1204 0.4859
1.4 0.7734 0.2266 0.0765 0.4960
1.5 0.7340 0.2660 0.0454 0.4986
1.6 0.6915 0.3085 0.0250 0.4933
1.7 0.6462 0.3538 0.0127 0.4806
1.8 0.5987 0.4013 0.0059 0.4609
1.9 0.5497 0.4503 0.0025 0.4352
2.0 0.5000 0.5000 0.0010 0.4045
2.1 0.4503 0.5497 0.0003 0.3702
2.2 0.4013 0.5987 0.0001 0.3336
2.3 0.3538 0.6462 0.0000 0.2959
2.4 0.3085 0.6915 0.0000 0.2584
2.5 0.2660 0.7340 0.0000 0.2221
2.6 0.2266 0.7734 0.0000 0.1880
2.7 0.1908 0.8092 0.0000 0.1566
2.8 0.1587 0.8413 0.0000 0.1285
2.9 0.1303 0.8697 0.0000 0.1038
3.0 0.1056 0.8944 0.0000 0.0825
3.1 0.0846 0.9154 0.0000 0.0646
3.2 0.0668 0.9332 0.0000 0.0498
3.3 0.0521 0.9479 0.0000 0.0378
3.4 0.0401 0.9599 0.0000 0.0282
3.5 0.0304 0.9696 0.0000 0.0207
3.6 0.0228 0.9772 0.0000 0.0150
3.7 0.0168 0.9832 0.0000 0.0107
3.8 0.0122 0.9878 0.0000 0.0075
3.9 0.0088 0.9912 0.0000 0.0052
4.0 0.0062 0.9938 0.0000 0.0035
n mean = 1.48 and sigma = 0.80
0.8
0.6
P
0.4
0.2
-2 -1 0 1 2 3 4 5 0
-5 -4 -3 -2 -1 0 1 2 3 4 5
Log count/g
Mean log count/g
PDF m Prob. acceptance m
P(accept)
Computed 5.00 %
Desired 5 %
ans and median

Geometric=median
30.4 cfu/g
ding mean and n
ing m in equivalent performance criterion

count to m
0.8
0.6
P
0.4
0.2
0
-5 -4 -3 -2 -1 0 1 2 3 4 5
Mean log count/g
Prob. acceptance m

mean 1.48 Computed 5.00 %
sigma 0.80 Target, left 5.00 %
m 2.53
n 30
c 0

99.9 3.10 3.96
Operating characteristic curve for proportion defective Probability density function (PDF) for
Pd and marginally acceptable Pm with n=5 and c=2 Distribution mean = 3.52 and sigm
1.00
0.6
0.90
0.80 0.5
0.70
0.4
0.60
0.50 0.3
P
0.40
Paccept 1 0.2
0.30 0
0.20 .2 0.1
0
0.10
0 .4 0
0.00 6 0 1 2 3 4 5 6
0.
0
.8
0.1
Log count/g
0.2
0
0.3
0.4
0.5
0.6
1 PDF m M
0.7
0.8
0.9
Pm 1 Pd
INPUTS
Batch acceptance for Pd mean 3.52
P(accept) sigma 0.8
Pd 20 % 26.9 % m 3
Pm 25 % M 4
actualPd 27.3 % n 5
actualPm 46.8 % 5.00 % c 2
Means and median

Arithmetic
17903.6 cfu/g
0 1 2 3 4 5
mean pa pm pd
3.5 0.2594 0.4679457 0.2726 0.0011749 0.0105965 0 0 0 0
0.5500 0.2500 0.2000 0.0503284 0.1143828 0.1 0 0 0
3.5 0.7411 0.2299418 0.0289 0.0499998 0 0 0 0 0
0.0 0.9999 8.813E-05 0.0000 0.999558 0.0004405 ### 0 0 0
0.1 0.9999 0.0001439 0.0000 0.9992778 0.0007193 ### 0 0 0
0.2 0.9998 0.0002316 0.0000 0.9988374 0.001157 ### 0 0 0
0.3 0.9996 0.0003672 0.0000 0.998156 0.0018333 ### 0 0 0
0.4 0.9994 0.0005736 0.0000 0.9971182 0.0028615 ### 0 0 0
0.5 0.9991 0.000883 0.0000 0.9955628 0.0043991 ### 0 0 0
0.6 0.9987 0.0013392 0.0000 0.9932687 0.00666 ### 0 0 0
0.7 0.9980 0.0020016 0.0000 0.98994 0.0099274 ### 0 0 0
0.8 0.9970 0.0029481 0.0000 0.9851897 0.0145656 ### 0 0 0
0.9 0.9957 0.0042791 0.0001 0.9785246 0.0210273 0 0 0 0
1.0 0.9938 0.0061212 0.0001 0.9693349 0.0298531 0 0 0 0
1.1 0.9912 0.00863 0.0001 0.9568908 0.0416553 0 0 0 0
1.2 0.9878 0.0119918 0.0002 0.9403539 0.0570807 0 0 0 0
1.3 0.9832 0.0164242 0.0004 0.9188067 0.0767422 0 0 0 0
1.4 0.9772 0.0221731 0.0006 0.8913086 0.1011158 0 0 0 0
1.5 0.9696 0.0295073 0.0009 0.856981 0.1303998 0 0 0 0
1.6 0.9599 0.0387093 0.0013 0.8151215 0.1643474 0 0 0 0
1.7 0.9479 0.0500611 0.0020 0.7653419 0.2020948 0 0 0 0
1.8 0.9332 0.0638274 0.0030 0.7077125 0.2420265 0 0 0 0
1.9 0.9154 0.0802333 0.0043 0.6428888 0.2817301 0 0 0 0
2.0 0.8944 0.0994401 0.0062 0.5721872 0.3180988 0.1 0 0 0
2.1 0.8697 0.12152 0.0088 0.4975779 0.3476216 0.1 0 0 0
2.2 0.8413 0.1464308 0.0122 0.4215702 0.3668583 0.1 0 0 0
2.3 0.8092 0.1739936 0.0168 0.3469879 0.3730396 0.2 0 0 0
2.4 0.7734 0.2038772 0.0228 0.2766585 0.3646649 0.2 0 0 0
2.5 0.7340 0.2355892 0.0304 0.2130704 0.3419352 0.2 0 0 0
2.6 0.6915 0.2684784 0.0401 0.1580677 0.3068695 0.2 0 0 0
2.7 0.6462 0.301749 0.0521 0.1126505 0.2630282 0.2 0 0 0
2.8 0.5987 0.3344865 0.0668 0.0769253 0.2148839 0.2 0 0 0
2.9 0.5497 0.3656961 0.0846 0.0502088 0.1669991 0.2 0 0 0
3.0 0.5000 0.3943502 0.1056 0.03125 0.1232344 0.2 0 0 0
3.1 0.4503 0.4194437 0.1303 0.0185065 0.0861994 0.2 0 0 0
3.2 0.4013 0.4400511 0.1587 0.0104067 0.0570588 0.1 0 0 0
3.3 0.3538 0.4553828 0.1908 0.0055459 0.0356883 0.1 0 0 0
3.4 0.3085 0.4648351 0.2266 0.002796 0.0210621 0.1 0 0 0
3.5 0.2660 0.4680289 0.2660 0.0013313 0.0117132 0 0 0 0
3.6 0.2266 0.4648351 0.3085 0.0005978 0.0061308 0 0 0 0
3.7 0.1908 0.4553828 0.3538 0.0002528 0.0030168 0 0 0 0
3.8 0.1587 0.4400511 0.4013 0.0001005 0.0013941 0 0 0 0
3.9 0.1303 0.4194437 0.4503 3.755E-05 0.0006044 0 0 0 0
4.0 0.1056 0.3943502 0.5000 1.316E-05 0.0002457 0 0 0 0
4.1 0.0846 0.3656961 0.5497 4.325E-06 9.351E-05 0 0 0 0
4.2 0.0668 0.3344865 0.5987 1.331E-06 3.332E-05 0 0 0 0
4.3 0.0521 0.301749 0.6462 3.832E-07 1.11E-05 0 0 0 0
4.4 0.0401 0.2684784 0.6915 1.032E-07 3.457E-06 ### 0 0 0
4.5 0.0304 0.2355892 0.7340 2.595E-08 1.006E-06 ### 0 0 0
4.6 0.0228 0.2038772 0.7734 6.094E-09 2.731E-07 ### 0 0 0
4.7 0.0168 0.1739936 0.8092 1.336E-09 6.919E-08 ### 0 0 0
4.8 0.0122 0.1464308 0.8413 2.73E-10 1.635E-08 ### 0 0 0
4.9 0.0088 0.12152 0.8697 5.201E-11 3.602E-09 ### 0 0 0
5.0 0.0062 0.0994401 0.8944 9.233E-12 7.393E-10 ### 0 0 0
5.1 0.0043 0.0802333 0.9154 1.526E-12 1.413E-10 ### 0 0 0
5.2 0.0030 0.0638274 0.9332 2.349E-13 2.516E-11 ### 0 0 0
5.3 0.0020 0.0500611 0.9479 3.364E-14 4.169E-12 ### 0 0 0
5.4 0.0013 0.0387093 0.9599 4.482E-15 6.427E-13 ### 0 0 0
5.5 0.0009 0.0295073 0.9696 5.554E-16 9.216E-14 ### 0 0 0
5.6 0.0006 0.0221731 0.9772 6.397E-17 1.229E-14 ### 0 0 0
5.7 0.0004 0.0164242 0.9832 6.848E-18 1.524E-15 ### 0 0 0
5.8 0.0002 0.0119918 0.9878 6.813E-19 1.756E-16 ### 0 0 0
5.9 0.0001 0.00863 0.9912 6.296E-20 1.88E-17 ### 0 0 0
6.0 0.0001 0.0061212 0.9938 5.404E-21 1.871E-18 ### 0 0 0
6.1 0.0001 0.0042791 0.9957 4.307E-22 1.728E-19 ### 0 0 0
6.2 0.0000 0.0029481 0.9970 3.187E-23 1.483E-20 ### 0 0 0
6.3 0.0000 0.0020016 0.9980 2.189E-24 1.182E-21 ### 0 0 0
6.4 0.0000 0.0013392 0.9987 1.395E-25 8.74E-23 ### 0 0 0
6.5 0.0000 0.000883 0.9991 8.251E-27 6E-24 ### 0 0 0
6.6 0.0000 0.0005736 0.9994 4.528E-28 3.822E-25 ### 0 0 0
6.7 0.0000 0.0003672 0.9996 2.305E-29 2.26E-26 ### 0 0 0
6.8 0.0000 0.0002316 0.9998 1.088E-30 1.239E-27 ### 0 0 0
6.9 0.0000 0.0001439 0.9999 4.766E-32 6.305E-29 ### 0 0 0
7.0 0.0000 8.813E-05 0.9999 1.935E-33 2.975E-30 ### 0 0 0
7.1 0.0000 5.316E-05 0.9999 7.287E-35 1.302E-31 ### 0 0 0
7.2 0.0000 3.16E-05 1.0000 2.544E-36 5.284E-33 ### 0 0 0
7.3 0.0000 1.85E-05 1.0000 8.232E-38 1.988E-34 ### 0 0 0
7.4 0.0000 1.067E-05 1.0000 2.469E-39 6.937E-36 ### 0 0 0
7.5 0.0000 6.062E-06 1.0000 6.865E-41 2.244E-37 ### 0 0 0
7.6 0.0000 3.393E-06 1.0000 1.769E-42 6.726E-39 ### 0 0 0
7.7 0.0000 1.871E-06 1.0000 4.224E-44 1.869E-40 ### 0 0 0
7.8 0.0000 1.016E-06 1.0000 9.347E-46 4.813E-42 ### 0 0 0
7.9 0.0000 5.436E-07 1.0000 1.916E-47 1.149E-43 ### 0 0 0
8.0 0.0000 2.864E-07 1.0000 3.641E-49 2.541E-45 ### 0 0 0
Calculations for 3-dimensional OC graph
pa pm pd
0 1 0 0 0 0 0 0 0
0 0.9 0.1 0 0 0 0 0 0
0.1 0.9 0 0.00001 0.00045 0 0 0 0
0 0.8 0.2 0 0 0 0 0 0
0.1 0.8 0.1 0.00001 0.0004 0 0 0 0
0.2 0.8 0 0.00032 0.0064 0.1 0 0 0
0 0.7 0.3 0 0 0 0 0 0
0.1 0.7 0.2 0.00001 0.00035 0 0 0 0
0.2 0.7 0.1 0.00032 0.0056 0 0 0 0
0.3 0.7 0 0.00243 0.02835 0.1 0 0 0
0 0.6 0.4 0 0 0 0 0 0
0.1 0.6 0.3 0.00001 0.0003 0 0 0 0
0.2 0.6 0.2 0.00032 0.0048 0 0 0 0
0.3 0.6 0.1 0.00243 0.0243 0.1 0 0 0
0.4 0.6 0 0.01024 0.0768 0.2 0 0 0
0 0.5 0.5 0 0 0 0 0 0
0.1 0.5 0.4 0.00001 0.00025 0 0 0 0
0.2 0.5 0.3 0.00032 0.004 0 0 0 0
0.3 0.5 0.2 0.00243 0.02025 0.1 0 0 0
0.4 0.5 0.1 0.01024 0.064 0.2 0 0 0
0.5 0.5 0 0.03125 0.15625 0.3 0 0 0
0 0.4 0.6 0 0 0 0 0 0
0.1 0.4 0.5 0.00001 0.0002 0 0 0 0
0.2 0.4 0.4 0.00032 0.0032 0 0 0 0
0.3 0.4 0.3 0.00243 0.0162 0 0 0 0
0.4 0.4 0.2 0.01024 0.0512 0.1 0 0 0
0.5 0.4 0.1 0.03125 0.125 0.2 0 0 0
0.6 0.4 0 0.07776 0.2592 0.3 0 0 0
0 0.3 0.7 0 0 0 0 0 0
0.1 0.3 0.6 0.00001 0.00015 0 0 0 0
0.2 0.3 0.5 0.00032 0.0024 0 0 0 0
0.3 0.3 0.4 0.00243 0.01215 0 0 0 0
0.4 0.3 0.3 0.01024 0.0384 0.1 0 0 0
0.5 0.3 0.2 0.03125 0.09375 0.1 0 0 0
0.6 0.3 0.1 0.07776 0.1944 0.2 0 0 0
0.7 0.3 0 0.16807 0.36015 0.3 0 0 0
0 0.2 0.8 0 0 0 0 0 0
0.1 0.2 0.7 0.00001 0.0001 0 0 0 0
0.2 0.2 0.6 0.00032 0.0016 0 0 0 0
0.3 0.2 0.5 0.00243 0.0081 0 0 0 0
0.4 0.2 0.4 0.01024 0.0256 0 0 0 0
0.5 0.2 0.3 0.03125 0.0625 0.1 0 0 0
0.6 0.2 0.2 0.07776 0.1296 0.1 0 0 0
0.7 0.2 0.1 0.16807 0.2401 0.1 0 0 0
0.8 0.2 0 0.32768 0.4096 0.2 0 0 0
0 0.1 0.9 0 0 0 0 0 0
0.1 0.1 0.8 0.00001 0.00005 ### 0 0 0
0.2 0.1 0.7 0.00032 0.0008 0 0 0 0
0.3 0.1 0.6 0.00243 0.00405 0 0 0 0
0.4 0.1 0.5 0.01024 0.0128 0 0 0 0
0.5 0.1 0.4 0.03125 0.03125 0 0 0 0
0.6 0.1 0.3 0.07776 0.0648 0 0 0 0
0.7 0.1 0.2 0.16807 0.12005 0 0 0 0
0.8 0.1 0.1 0.32768 0.2048 0.1 0 0 0
0.9 0.1 0 0.59049 0.32805 0.1 0 0 0
-0.00001 0.00001 1 -1E-25 5E-25 ### 0 0 0
0.09999 0.00001 0.9 9.995E-06 4.998E-09 ### 0 0 0
0.19999 0.00001 0.8 0.0003199 7.998E-08 ### 0 0 0
0.29999 0.00001 0.7 0.0024296 4.049E-07 ### 0 0 0
0.39999 0.00001 0.6 0.0102387 1.28E-06 ### 0 0 0
0.49999 0.00001 0.5 0.0312469 3.125E-06 ### 0 0 0
0.59999 0.00001 0.4 0.0777535 6.48E-06 ### 0 0 0
0.69999 0.00001 0.3 0.168058 1.2E-05 ### 0 0 0
0.79999 0.00001 0.2 0.3276595 2.048E-05 ### 0 0 0
0.89999 0.00001 0.1 0.5904572 3.28E-05 ### 0 0 0
0.99999 0.00001 0 0.99995 5E-05 ### 0 0 0
n mean = 3.52 and sigma = 0.80
0.8
0.6
P
0.4
0.2
2 3 4 5 6 7 8 9 0
0 1 2 3 4 5 6 7 8 9
Log count/g
Mean log count/g
PDF m M Prob. acceptance m M
P(accept)
Computed 5.00 %
Desired 5 %
ans and median

Geometric=median
3281.8 cfu/g
6 7 8 9
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
count to m
0.8
0.6
P
0.4
0.2
0
0 1 2 3 4 5 6 7 8 9
Mean log count/g
Prob. acceptance m M

mean 3.52 Computed 5.00 %
sigma 0.80 Target, left 5.00 %
m 4.03
M 5.03
n 10
c 0

99.9 3.10 5.99
10
P(accept)
0 0.0500 <= Used by macros for finding mean and n
0 0.2687 <= Used in test against ICMSF tables
0 0.0500 <= Used by macro for finding m in equivalent performance criterion
0 1.0000 0.0000
0 1.0000 0.0001
0 1.0000 0.0001
0 1.0000 0.0002
0 1.0000 0.0003
0 1.0000 0.0004
0 0.9999 0.0006
0 0.9999 0.0010
0 0.9998 0.0016
0 0.9997 0.0024
0 0.9996 0.0035
0 0.9993 0.0052
0 0.9988 0.0075
0 0.9981 0.0108
0 0.9970 0.0151
0 0.9953 0.0208
0 0.9927 0.0283
0 0.9888 0.0379
0 0.9828 0.0500
0 0.9740 0.0648
0 0.9610 0.0828
0 0.9423 0.1041
0 0.9161 0.1289
0 0.8804 0.1570
0 0.8336 0.1884
0 0.7745 0.2226
0 0.7032 0.2589
0 0.6213 0.2964
0 0.5319 0.3341
0 0.4394 0.3707
0 0.3489 0.4050
0 0.2653 0.4356
0 0.1926 0.4612
0 0.1331 0.4808
0 0.0873 0.4935
0 0.0543 0.4986
0 0.0319 0.4959
0 0.0177 0.4857
0 0.0092 0.4682
0 0.0045 0.4444
0 0.0021 0.4153
0 0.0009 0.3821
0 0.0004 0.3460
0 0.0001 0.3086
0 0.0000 0.2709
0 0.0000 0.2341
0 0.0000 0.1992
0 0.0000 0.1668
0 0.0000 0.1376
0 0.0000 0.1117
0 0.0000 0.0893
0 0.0000 0.0702
0 0.0000 0.0544
0 0.0000 0.0415
0 0.0000 0.0312
0 0.0000 0.0230
0 0.0000 0.0168
0 0.0000 0.0120
0 0.0000 0.0085
0 0.0000 0.0059
0 0.0000 0.0040
0 0.0000 0.0027
0 0.0000 0.0018
0 0.0000 0.0012
0 0.0000 0.0008
0 0.0000 0.0005
0 0.0000 0.0003
0 0.0000 0.0002
0 0.0000 0.0001
0 0.0000 0.0001
0 0.0000 0.0000
0 0.0000 0.0000
0 0.0000 0.0000
0 0.0000 0.0000
0 0.0000 0.0000
0 0.0000 0.0000
0 0.0000 0.0000
0 0.0000 0.0000
0 0.0000 0.0000
0 0.0000 0.0000
0 0.0000 0.0000
Table for OC graph
0 0.0000 Pd 0 0
0 0.0000 1.00 0.00
0 0.0086 0.90 0.00
0 0.0000 0.80 0.00
0 0.0068 0.70 0.00
0 0.0579 0.60 0.01
0 0.0000 0.50 0.03
0 0.0053 0.40 0.08
0 0.0451 0.30 0.17
0 0.1631 0.20 0.33
0 0.0000 0.10 0.59
0 0.0039 0.00 1.00
0 0.0339
0 0.1239
0 0.3174
0 0.0000
0 0.0028
0 0.0243
0 0.0902
0 0.2342
0 0.5000
0 0.0000
0 0.0018
0 0.0163
0 0.0618
0 0.1638
0 0.3563
0 0.6826
0 0.0000
0 0.0011
0 0.0099
0 0.0389
0 0.1062
0 0.2375
0 0.4666
0 0.8369
0 0.0000
0 0.0005
0 0.0051
0 0.0213
0 0.0614
0 0.1438
0 0.2938
0 0.5454
0 0.9421
0 0.0000
0 0.0002
0 0.0019
0 0.0092
0 0.0294
0 0.0750
0 0.1642
0 0.3224
0 0.5837
0 0.9914
0 0.0000
0 0.0000
0 0.0003
0 0.0024
0 0.0102
0 0.0312
0 0.0778
0 0.1681
0 0.3277
0 0.5905
0 1.0000
Coordinates to place m
proportion m
0 3 0
0.5485 3 1
Coordinates to place M
proportion M
0 4 0
0.5485 4 1
Coordinates to place Pd
p accept
x y
0.2726 0.0000
0.2726 5.0000
ivalent performance criterion

Pm
0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1
0.00
0.00 0.00
0.00 0.00 0.00
0.01 0.01 0.00 0.00
0.03 0.02 0.01 0.00 0.00
0.08 0.06 0.04 0.02 0.00 0.00
0.16 0.14 0.11 0.06 0.02 0.00 0.00
0.32 0.29 0.24 0.16 0.09 0.03 0.01 0.00
0.58 0.55 0.47 0.36 0.23 0.12 0.05 0.01 0.00
0.99 0.94 0.84 0.68 0.50 0.32 0.16 0.06 0.01 0.00

Microbiological Sampling Plans: A Tool To Explore ICMSF Recommendations

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Microbiological sampling plans: a tool to explore ICMSF recommendations

Version 1.01 Version 2.01: Addition of enrichment calculations

Version 2.02: Simpliciation and reorganisation

Version 2.03-2.05: Finilisation for posting

n Microbiological Specifications for Foods (ICMSF)

Downloading the spreadsheet and technical issues

8) FOR PC/Windows USERS:

9) FOR Macintosh USERS:

For Users with recent Macintosh computers

For Users with recent Macintosh computers

For a manual please open the following word-file

contamination), yet the lot will be accepted

Sandbox: for your own calculations

Means and median

Means and median

ding mean and n

ing m in equivalent performance criterion

ALTERNATIVE n AND c P(accept)

Implied Acceptance level

Sandbox: for your own calculations

Means and median

ans and median

ding mean and n

ing m in equivalent performance criterion

ALTERNATIVE n AND c P(accept)

Implied Acceptance level

Sandbox: for your own calculations

Means and median

Calculations for 3-dimensional OC graph

ans and median

ALTERNATIVE n AND c P(accept)

Implied Acceptance level

Table for OC graph

ivalent performance criterion

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