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Homeostasis Lab: Water Content

By: Hunter Gray, Joaquin Arellanes, Jovais Kennedy, John Kosta

Abstract:
For this lab, the first step that was done was to plan out the whole project. We came up
with an idea that would test homeostasis by measuring water levels in our bodies. All three
participants would run 800m and drink different amounts of water to see how their water content
would fluctuate and then go back to normal. After this, the participants had to go to the track to
test homeostasis. Then, we collected results and thought about what they meant and if we took
the right data. In the end we had to go back and weigh ourselves a second time. After making
revisions we made graphs and tables to express our data.

Introduction:
Homeostasis is how the body maintains balance. Whether that be water content, body
heat, energy, etc. Homeostasis uses different organs and hormones to regulate the body. When
you exercise you use water to cool yourself. Our body has to regulate this water use so it
doesnt accidentally use all of it. Our kidneys, skin, gastrointestinal, and respiratory tract
maintain our water balance by controlling the water concentration of our blood plasma. It also
controls salt levels. The hormone anti-diuretic is what makes us thirsty and what makes us have
to go to the bathroom. People actively sweat and pee to relieve ourselves of water and cool
ourselves. To replenish this people rely on our kidneys and drinking water. While gaining and
losing water, people gain and lose weight accordingly. Our hypothesis is the more water you
drink, the less weight youll lose and if you drink less water, youll lose more. Everyone will lose
weight if they run. Our plan was to test how peoples water content would fluctuate and keep
balance on and after a 800m run depending on how much water they drink.

Procedure and materials:

Procedure:
1. Go on a 400m track
2. Have participant 2 drink 1000ml (2 bottles) of water and the other two participants drink
nothing
3. Weigh all three participants and record their weights
4. All three participants run 400m (1 lap)
5. After the first lap, have participant #3 quickly drink 250ml ( bottle) of water and weigh
all three participants upon completion of the lap. Weigh participant #3 after they drink
the water.
6. Have all three participants complete the second lap and then record all of their weights,
again making sure participant #3 drinks 250ml ( bottle) of water before weighing.
7. Wait 10 minutes and weigh all three participants
Materials:
1. Human scale
2. 3 full 500ml water bottles
3. People to test
4. A 400m track
5. notebook and writing utensil

Results:
Before run (lbs.) After first lap After 2 laps (lbs.) 4 days after run
(lbs.)

Participant #1 141 lbs. 142 lbs. 143 lbs. 141 lbs.


(no water)

Participant #2 148.5 lbs. 150 lbs. 148bs. 149 lbs.


(1000 ml before
run)

Participant #3 214 lbs. 215 lbs. 216 lbs. 210 lbs.


(500 ml, 250
after first lap,
250 after second
lap)
Commented [1]: I feel like line graph to graph weight
would be helpful

Discussion:
Participant 3s (500 ml) weight fluctuated the most because lost weight over the
weekend and sweated a bunch of water out. Participant 2(1000 ml) fluctuated the second most
because they drank a lot of water so his weight went up significantly and then it naturally came
back down. Participant 1 changed the least because they drank no water and hovered around
their normal body weight the whole time. Discovered that homeostasis occurs in the body
through balancing out water levels and no matter how much one drank, their body will find a
way to regulate their weight back to normal through sweating, urinating, breathing, etc. Data
was acquired fairly quickly. All participants were weighed and it made sense to homeostasis.
After the run, homeostasis by seeing our weight go back down. Participants were not
weighed after they lost the water so homeostasis was not proved yet. This was fixed by
weighing ourselves 4 days after when they had lost the water that they took in. Next time the lab
could heavily be improved improved by taking more data. Ways we could take more data are
seeing how much off the water loss was sweat by weighing our clothes while they were dry and
then when they are sweaty. seeing how much they peed out by taking the volume of it. Then the
rest of the water loss would be through breathing. Other, new investigations how the speed of
someone running effects homeostasis, how someones physical shape effects homeostasis,
and how the weather outside effects homeostasis.

Works cited:
http://www.h4hinitiative.com/everyday-hydration/water-balance-regulation

https://www.merckmanuals.com/home/hormonal-and-metabolic-disorders/water-balance/about-
body-water