Annals of Agri Bio Research

Volume 17, Issue 2, December 2012, Pages 87-92

In vitro plant regeneration in Salvadora

oleoides Decne from cotyledonary node
Singh, N.a , Laura, J.S.b, Kumar, S.b
a
Department of Botany, Kurukshetra University, Kurukshetra (Haryana), India
b
Department of Environmental Sciences, M. D. University, Rohtak-124001(Haryana), India

View references (24)

Abstract
An efficient and reproducible protocol for micropropagation of Salvadora oleoides Decne has
been standardized. Seeds were germinated on half strength MS medium. Cotyledonary node
explants (5-7 mm long) excised from 12-15 days old aseptically grown seedlings were cultured
on MS medium supplemented with different concentrations of cytokinins alone and in
combination with auxins. Maximum shoot proliferation from single explant was obtained on
MS medium fortified with 4.0 mg/1 BAP and 0.5 mg/1 NAA. In vitro regenerated shoots
induced roots on MS medium supplemented with 1.0 mg/1 NAA. The regenerants were
transferred to field conditions after acclimatization with 80% survival rate.

Author keywords
Auxins; Cotyledonary node; Cytokinins; Micropropagation; Salvadora oleoide

Indian Science Abstract

LIST OF PERIODICALS COVERED IN THIS ISSUE

ISA VOLUME:49 ISA ISSUE:19 DATE:01 /Oct /2013

019819 Singh N;Laura J S;Surender Kumar

Botany Dep, Kurukshetra Univ, Kurukshetra, Haryana ,
In vitro plant regeneration in Salvadora oleoides decne from cotyledonary node , 2012,17(2),87-92