Voltase terkecil yg menyebabkan sanggunp ontraksi itu threshold voltage

Exercise 3: Neurophysiology of Nerve Impulses: Activity 1: The Resting

Membrane Potential Lab Report
Pre-lab Quiz Results
You scored 100% by answering 4 out of 4 questions correctly.

1. What is the approximate concentration of K+ inside a typical cell

(intracellular concentration)?
You correctly answered: a. 150 mM
2. What is the approximate concentration of K+ outside a cell
(extracellular concentration)?
You correctly answered: b. 5 mM
3. What is the approximate concentration of Na+ inside a cell
(intracellular concentration)?
You correctly answered: b. 5 mM
4. What is the approximate concentration of Na+ outside a cell
(extracellular concentration)?
You correctly answered: a. 150 mM

Experiment Results
Predict Question:
Predict Question: Predict what will happen to the resting membrane
potential if the extracellular K+ concentration is
increased.
Your answer : b. The resting membrane potential will become less
negative.
Stop & Think Questions:
What is the polarity of the resting membrane potential (voltage)?
You correctly answered: b. negative
What does it mean that the voltage just inside the membrane is negative?
You correctly answered: b. There are more negative charges than positive
charges just inside the membrane.
The membrane of most cells, including neurons, contains passive, open,
K+ leak channels. Given the normal K+ concentrations and the resultant
concentration gradient, which direction would K+
be expected to move (diffuse) through these leak channels?
You correctly answered: b. out of the cell
What effect does increasing extracellular K+ have on the net diffusion of
K+ out of the cell?
You correctly answered: b. It decreases the net diffusion of K+ .
Which way would Na+ move across the membrane if there were open Na+
channels?
You correctly answered: a. Na+ would diffuse into the cell.
The membrane has open K+ channels, and changing extracellular K+
concentration results in a change in membrane
potential. Changing the extracellular Na+ concentration does not
significantly change the membrane potential. What do
your results suggest about the number or state (open or closed) of Na+
channels in the resting membrane of a neuron?
You correctly answered: b. Na+ channels are mostly closed.
Experiment Data:
Extracellular Fluid (ECF) Microelectrode Position Voltage (mV)
Control Cell body extracellular 0
Control Cell body intracellular -70
Control Axon extracellular 0
Control Axon intracellular -70
High K+ Axon intracellular -40
High K+ Axon extracellular 0
High K+ Cell body extracellular 0
High K+ Cell body intracellular -40
Low Na+ Cell body intracellular -72
Low Na+ Cell body extracellular 0
Low Na+ Axon extracellular 0
Low Na+ Axon intracellular -72

Post-lab Quiz Results

You scored 100% by answering 3 out of 3 questions correctly.

1. A negative membrane potential was recorded when the tip of the

microelectrode was
You correctly answered: d. both inside the cell body and inside the
axon.
2. Which of the following caused a change in membrane potential from
-70 to -40 in the cell body?
You correctly answered: a. an increase in extracellular K+
3. Which of the following has the most negative voltage?
You correctly answered: c. between the inside of the axon and the
outside of the axon with control K+ ECF

Review Sheet Results

1. Explain why increasing extracellular K+ reduces the net diffusion of

K+ out of the neuron through the K+ leak
channels.
Your answer:
Increasing extracellular k+ makes difference of k+ concentration less
steeper. So less k+ diffuse out of the neuron.
2. Explain why increasing extracellular K+ causes the membrane
potential to change to a less negative value. How well
did the results compare with your prediction?
Your answer:
 Less k+ diffuse out of the neuron. So more k+ remains in the neuron
in comparing to control. As a result it makes a less
negative value.
3. Explain why a change in extracellular Na+ did not significantly alter
the membrane potential in the resting neuron?
Your answer:
Because almost all of Na+ channels were closed, most of Na+ did not
diffused into the neuron. So there was no significant
change in membrane potential.
4. Discuss the relative permeability of the membrane to Na+ and K+ in
a resting neuron.
Your answer:
K+ diffuse from high gradient to low gradient because most most of
k+ channel is opend. Otherwise Na+ does not show
outstanding movement.
5. Discuss how a change in Na+ or K+ conductance would affect the
resting membrane potential.
Your answer:
When conductance of k+ increased, membrane potential becomes
more negative. When conductance of k+ decreased,
membrane potential becomes less negative.
In Na+ it works in the other way.