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Translation of the original operating manual

sb
08/2012
Rev. 3.10 of 06/04/2016
Sigma 1-14; ;

part no. 0700002

Sigma 1-14

from serial no. 146194

Laboratory centrifuge

Operating Manual

Please retain for later use!


Laboratory centrifuge Sigma 1-14

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Translation of the original operating manual, part no. 0700002
Laboratory centrifuge Sigma 1-14

In case of inquiries, please state the following numbers:

Order number:

Serial number:

Copyright by
Sigma Laborzentrifugen GmbH
An der Unteren Sse 50
37520 Osterode am Harz
Germany

Tel.: +49 (0) 5522 / 5007-0


Fax: +49 (0) 5522 / 5007-12
Web: www.sigma-zentrifugen.de
E-mail: info@sigma-zentrifugen.de

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Translation of the original operating manual, part no. 0700002
Laboratory centrifuge Sigma 1-14

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Translation of the original operating manual, part no. 0700002
Laboratory centrifuge Sigma 1-14

Table of contents

1 General information ............................................................................................................. 9


1.1 Importance of the operating manual.................................................................................. 9
1.2 Intended use ..................................................................................................................... 9
1.3 Warranty and liability......................................................................................................... 9
1.4 Copyright ........................................................................................................................ 10
1.5 Standards and regulations .............................................................................................. 10
1.6 Scope of supply .............................................................................................................. 10
2 Layout and mode of operation .......................................................................................... 11
2.1 Layout of the centrifuge .................................................................................................. 11
2.1.1 Functional and operating elements ............................................................................ 11
2.1.2 Name plate ................................................................................................................ 12
2.2 Mode of operation ........................................................................................................... 13
2.2.1 Centrifugation principle .............................................................................................. 13
2.2.2 Area of application ..................................................................................................... 13
2.2.2.1 Speed, radius, and relative centrifugal force ...................................................... 14
2.2.2.2 Density ............................................................................................................... 14
3 Safety .................................................................................................................................. 15
3.1 Marking of the unit .......................................................................................................... 15
3.2 Explanation of the symbols and notes ............................................................................. 16
3.3 Responsibility of the operator.......................................................................................... 17
3.4 Operating personnel ....................................................................................................... 17
3.5 Informal safety instructions ............................................................................................. 17
3.6 Safety instructions .......................................................................................................... 18
3.6.1 Electrical safety ......................................................................................................... 18
3.6.2 Mechanical safety ...................................................................................................... 18
3.6.3 Fire prevention........................................................................................................... 19
3.6.4 Chemical and biological safety .................................................................................. 19
3.6.5 Safety instructions for centrifugation .......................................................................... 20
3.6.6 Resistance of plastics ................................................................................................ 20
3.6.7 Service life of rotors and accessories......................................................................... 21
3.7 Safety devices ................................................................................................................ 22
3.7.1 Lid lock device ........................................................................................................... 22
3.7.2 System check ............................................................................................................ 22
3.8 Measures in the event of hazards and accidents ............................................................ 22
3.9 Remaining hazards ......................................................................................................... 22
4 Storage and transport ....................................................................................................... 23
4.1 Dimensions and weight ................................................................................................... 23
4.2 Storage conditions .......................................................................................................... 23
4.3 Notes on transport .......................................................................................................... 23
4.4 Packaging ....................................................................................................................... 24
4.5 Transport safety device................................................................................................... 24

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Table of contents
5 Set-up and connection ...................................................................................................... 25
5.1 Installation site ................................................................................................................ 25
5.2 Power supply .................................................................................................................. 25
5.2.1 Type of connection .................................................................................................... 25
5.2.2 Customer-provided fuses ........................................................................................... 25
6 Using the centrifuge .......................................................................................................... 26
6.1 Initial start-up .................................................................................................................. 26
6.2 Switching the centrifuge on ............................................................................................. 26
6.2.1 Opening and closing the lid ........................................................................................ 26
6.2.2 Installation of rotors and accessories ......................................................................... 27
6.2.2.1 Installation of the rotor........................................................................................ 27
6.2.2.2 Installation of the microhaematocrit rotor............................................................ 28
6.2.2.3 Installation of accessories .................................................................................. 29
6.2.2.4 Tubes................................................................................................................. 29
6.3 Control System "Spincontrol Basic" ................................................................................ 30
6.3.1 User interface ............................................................................................................ 30
6.3.2 Display....................................................................................................................... 30
6.3.3 Starting a centrifugation run ....................................................................................... 31
6.3.4 Interrupting a centrifugation run ................................................................................. 31
6.3.5 Interrupting a deceleration process ............................................................................ 31
6.3.6 Speed / Relative centrifugal force (RCF).................................................................... 31
6.3.6.1 Changing the speed/RCF value during centrifugation ........................................ 31
6.3.7 Runtime ..................................................................................................................... 32
6.3.7.1 Changing the runtime during centrifugation ........................................................ 32
6.3.7.2 Short run (quick run) .......................................................................................... 32
6.3.7.3 Continuous run................................................................................................... 33
6.3.8 Softstart and softstop function ................................................................................... 33
6.3.9 Rotor selection........................................................................................................... 34
6.4 Switching the centrifuge off ............................................................................................. 34
7 Malfunctions and error correction .................................................................................... 35
7.1 General malfunctions ...................................................................................................... 35
7.1.1 Emergency lid release ............................................................................................... 36
7.2 Table of error codes ........................................................................................................ 37
7.3 Service contact ............................................................................................................... 38

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Table of contents
8 Maintenance and service................................................................................................... 39
8.1 Maintenance ................................................................................................................... 39
8.1.1 Centrifuge .................................................................................................................. 39
8.1.2 Accessories ............................................................................................................... 40
8.1.2.1 Plastic accessories ............................................................................................ 41
8.1.3 Rotors ........................................................................................................................ 41
8.1.4 Microhaematocrit rotor ............................................................................................... 41
8.1.5 Glass breakage ......................................................................................................... 42
8.2 Sterilisation and disinfection of the rotor chamber and accessories ................................ 42
8.2.1 Autoclaving ................................................................................................................ 43
8.3 Service ........................................................................................................................... 44
8.4 Return of defective parts ................................................................................................. 45
9 Disposal.............................................................................................................................. 47
9.1 Disposal of the centrifuge ............................................................................................... 47
9.2 Disposal of the packaging ............................................................................................... 47
10 Technical data .................................................................................................................... 48
10.1 Ambient conditions ......................................................................................................... 48
10.2 Technical documentation ................................................................................................ 49
11 Appendix ............................................................................................................................ 50
11.1 Range of accessories ..................................................................................................... 50
11.1.1 Rotor radii .................................................................................................................. 51
11.2 Speed-gravitational-field-diagram ................................................................................... 52
11.3 Table of the service life of rotors and accessories........................................................... 53
11.4 Resistance data .............................................................................................................. 54
11.5 EC Declaration of Conformity.......................................................................................... 59
12 Index ................................................................................................................................... 61

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Table of contents

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1 General information
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1 General information
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1.1 Importance of the operating manual


A fundamental requirement for the safe and trouble-free operation of the
centrifuge is to be familiar with the fundamental safety instructions and all
possible hazards.
The operating manual includes important information concerning the safe
operation of the centrifuge.
This operating manual and, in particular, the notes on safety and hazards
must be observed by all persons operating the centrifuge.
In addition, the local rules and regulations for the prevention of accidents
must be complied with.
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1.2 Intended use


Centrifuges are power-driven machines that separate liquids from solid
matter, liquid mixtures, or solid mixtures by centrifugal force. They are
solely intended for this purpose. Any other use beyond this area of
application is regarded as improper use. Sigma Laborzentrifugen GmbH
cannot be held liable for any damage resulting from such improper use.
The intended use also includes
observation of all the notes and instructions included in the operating
manual and
compliance with the care, cleaning, and maintenance instructions.
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1.3 Warranty and liability


The warranty and liability are subject to our "General Conditions" that were
distributed to the operator upon the conclusion of the contract.
Warranty and liability claims are excluded if they are due to:
improper use.
non-compliance with the safety instructions and hazard warnings in the
operating manual.
improper installation, start-up, operation, or maintenance of the
centrifuge.
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1 General information
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1.4 Copyright
The copyright concerning the operating manual remains with
Sigma Laborzentrifugen GmbH.
The operating manual is solely intended for the operator and their
personnel. It includes instructions and information that must not be
duplicated,
distributed, or
communicated in any other way.
Non-compliance may be prosecuted under criminal law.
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1.5 Standards and regulations


EC declaration of conformity
(see chapter 11.5 - "EC Declaration of Conformity")
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1.6 Scope of supply


The centrifuge comprises:
1 connection cable depending on the voltage variant
1 spanner for emergency lid release part no. 930 014
1 rotor fastening nut part no. 80 301

Documentation
Operating manual incl. EC declaration of conformity
(see chapter 11.5 - "EC Declaration of Conformity")

Accessories
according to your order, our order confirmation, and our delivery note.
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2 Layout and mode of operation


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2 Layout and mode of operation


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2.1 Layout of the centrifuge


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2.1.1 Functional and operating elements

1 Lid
2 Display
3 User interface
(see chapter 6.3.1 -
"User interface")

Fig. 1: Total view of the centrifuge

4 Name plate (see chapter


2.1.2 - "Name plate")
5 Mains power switch
6 Mains power input

Fig. 2: Rear view of the centrifuge


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2.1.2 Name plate

1 Manufacturer and
registered office
2 Type
3 Serial number
4 Max. speed
5 Max. kinetic energy
6 Max. density
7 Nominal voltage
8 Input fuse
9 Symbol for special
disposal (see chapter
9 - "Disposal")
10 CE-mark in
compliance with the
directive 2006/42/EG
11 Part number
12 Year of manufacture
13 Power consumption
Fig. 1: Example of a name plate
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2.2 Mode of operation


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2.2.1 Centrifugation principle


Centrifugation is a process for the separation of heterogeneous mixtures of
substances (suspensions, emulsions, or gas mixtures) into their
components. The mixture of substances, which rotates on a circular path, is
subject to centripetal acceleration that is several times greater than the
gravitational acceleration.
Centrifuges use the mass inertia inside the rotor chamber for separating the
substances. Due to their higher inertia, particles or media with a higher
density travel outwards. In doing so, they displace the components with a
lower density, which in turn travel towards the centre.
The centripetal acceleration of an object inside a centrifuge, as the effect of
centripetal force, depends on the distance between the object and the axis
of rotation as well as on the angular velocity. It increases linearly as a
function of the distance with regard to the axis of rotation and quadratically
as a function of the angular velocity. The bigger the radius in the rotor
chamber is and the higher the speed is, the higher the centripetal
acceleration is. However, the forces acting on the rotor also increase.
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Das

2.2.2 Area of application


Depending on the area of application of the centrifuge and also on the
particle size, solids content, and volume throughput of the mixture of
substances that is to be centrifuged, there are different types of centrifuges.
The areas of application go from household use as a salad spinner or
honey separator up to specialised technical applications in the clinical,
biological, or biochemical context:
For numerous clinical examinations, cellular material must be separated
from the liquid to be analysed. The normal separation process can be
sped up considerably by using laboratory centrifuges.
In the metal-working industry, centrifuges are used for separating oil
from metal cuttings. Dairies use centrifuges in order to separate cows
milk into cream and low-fat milk.
Particularly big centrifuges are used in the sugar industry for separating
the syrup from the crystalline sugar.
Ultracentrifuges are predominantly used in biology and biochemistry in
order to isolate particles, e.g. viruses. They are specifically designed for
high speeds up to 500,000 rpm. The rotor moves in a vacuum in order
to avoid air friction.
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2.2.2.1 Speed, radius, and relative centrifugal force


The acceleration g, which the samples are subject to, can be increased by
increasing the radius in the rotor chamber and by increasing the speed.
These three parameters are interdependent and linked with each other via
the following formula:
Relative centrifugal force RCF = 11.18 x 10-6 x r x n2
r = radius in cm
n = speed in rpm
RCF without any dimension
If two values are entered, the third value is determined by way of the stated
formula. If, afterwards, the speed or the radius is changed, the resulting
relative centrifugal force will be recalculated automatically by the control
unit. If the RCF is changed, the speed will be adapted while the specified
radius is maintained.
The speed-gravitational-field-diagram provides an overview of the
relationship between speed, radius, and RCF (see chapter 11.2 - "Speed-
gravitational-field-diagram").
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2.2.2.2 Density
The laboratory centrifuge is suitable for the separation of constituents of
different densities in mixtures with a maximum density of 1.2 g/cm3. All
information concerning the speed of rotors and accessories refers to liquids
with a density corresponding to this specification. If the density is above this
value, the maximum permissible speed of the centrifuge must be reduced
based on the following formula:
(1,2 / Rho)
n = nmax x
3
Rho = density in g/cm
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3 Safety
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3 Safety
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3.1 Marking of the unit


The following symbols are used for all types of centrifuges manufactured by Sigma:

Dangerous voltage
I On (Power)

Hot surface
0 Off (Power)

Name plate (see chapter


Caution! Risk of bruising
2.1.2 - "Name plate")

CE mark in compliance with


Protective earth (ground)
the directive 2006/42/EC

Do not dispose as part of


Earth (ground)
domestic waste

GS mark (tested safety;


Unplug the mains plug
only for Germany)

Arrow indicating the NRTL mark (only for the


direction of rotation USA and Canada)

RCM mark
4C Guarantee
(only for Australia)

Label indicating the


emergency release system

Safety indications on the centrifuge must be kept readable at all times. If


necessary, they must be replaced.
NOTE

Not all of the symbols/labels are used for this centrifuge type.

NOTE
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3.2 Explanation of the symbols and notes


In this operating manual, the following names and symbols to indicate
hazards are used:

This symbol stands for a direct hazard to the life and health of persons.

Non-observance of these symbols causes serious health problems up to


DANGER life-endangering injuries.

This symbol stands for a direct hazard to the life and health of persons
due to electrical voltage.

Non-observance of these symbols causes serious health problems up to


DANGER life-endangering injuries.

This symbol stands for a potential hazard to the life and health of persons.

Non-observance of these symbols can cause serious health problems up


WARNING to life-endangering injuries.

This symbol indicates a potentially hazardous situation

Non-observance of these notes can cause minor injuries or damage to


CAUTION property.

This symbol indicates important information.

NOTE
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3.3 Responsibility of the operator


The operator is responsible for authorising only qualified personnel to work
on the centrifuge (see chapter 3.4 - "Operating personnel").
The areas of responsibility of the personnel concerning the operation,
maintenance, and care of the unit must be clearly defined.
The safety-conscious work of the personnel in compliance with the
operating manual and the relevant EC and national health and safety
regulations as well as with the accident prevention regulations must be
checked at regular intervals (e.g. every month).
Under the international rules for health and safety at work, the operator is
obliged to:
take measures in order to prevent all danger to life or health during
work.
ensure that centrifuges are operated properly and entirely as intended
(see chapter 1.2 - "Intended use").
take protective measures against fire and explosion when working with
hazardous substances.
take measures for the safe opening of centrifuges.
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3.4 Operating personnel


Persons operating the unit must
be familiar with the fundamental regulations concerning workplace
safety and accident prevention
have read and understood this operating manual (and in particular the
safety sections and warning notes) and confirmed this with their
signature.
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3.5 Informal safety instructions


This operating manual is a part of the product.
The operating manual must be kept at the location of use of the
centrifuge. Ensure that it is accessible at all times.
The operating manual must be handed over to any subsequent owner
or operator of the centrifuge.
Any changes made must be added to the operating manual.
In addition to the operating manual, the general and local rules and
regulations concerning the prevention of accidents and the protection of
the environment must also be supplied.
Safety and danger indications on the centrifuge must be kept readable
at all times. If necessary, they must be replaced.
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3.6 Safety instructions


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3.6.1 Electrical safety


To reduce the risk of electrical shock, the centrifuge uses a three-wire
electrical cord and plug to connect the equipment to earth-ground. To
preserve this safety feature:
Ensure that the wall socket is properly wired and grounded.
Check that the mains voltage agrees with the nominal voltage listed on
the name plate.
Do not place vessels containing liquid on the centrifuge lid or within the
DANGER safety distance of 30 cm around the centrifuge. Spilled liquids may get
into the centrifuge and damage electrical or mechanical components.
Work on the power supply system must only be performed by certified
electricians.
Inspect the electrical equipment of the unit regularly. Defects such as
loose or burnt cables must be eliminated immediately.
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3.6.2 Mechanical safety


In order to ensure the safe operation of the centrifuge, observe the
following:
Do not open the lid when the rotor is in motion!
Do not reach into the rotor chamber when the rotor is in motion!
Do not use the centrifuge if it was installed incorrectly.
Do not use the centrifuge without panels.
WARNING
Do not use the centrifuge if the rotors and inserts show signs of
corrosion or other defects.
Only use the centrifuge with rotors and accessories that have been
approved by the manufacturer. In case of doubt, contact the
manufacturer (see chapter 7.3 - "Service contact").
Do not hold your fingers between the lid and the housing when closing
the lid. Risk of crushing!
Defective lid relieving devices could cause the centrifuge lid to fall
(contact the service department, if necessary). Risk of crushing!
Do not hit or move the centrifuge during its operation.
Do not lean against or rest on the centrifuge during its operation.
Do not spin any substances that could damage the material of the
rotors and buckets of the centrifuge in any way. Highly corrosive
substances, for example, damage the material and affect the
mechanical strength of the rotors and buckets.
Stop the centrifuge immediately in the event of a malfunction. Eliminate
the malfunction (see chapter 7 - "Malfunctions and error correction") or
inform the service department of the manufacturer (see chapter 7.3 -
"Service contact").
Ensure that all repairs are performed only by authorised and
specialised personnel.

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3 Safety

Prior to any start-up, check the centrifuge, rotor, and accessories for
signs of damage that can be discerned from the outside. Special
attention must be paid to all of the rubber parts (e.g. motor cover, lid
seal, and adapters) in terms of visible structural changes. Defective
WARNING parts must be replaced immediately.
Open the centrifuge when it is not in use so that moisture can
evaporate.
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3.6.3 Fire prevention

Always use fuses with the same type and rating specified.
Do not spin explosive or inflammable substances.
Do not use the centrifuge within hazardous locations.
DANGER
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3.6.4 Chemical and biological safety


If pathogenic, toxic, or radioactive samples are intended to be used in the
centrifuge, it is in the responsibility of the user to ensure that all necessary
safety regulations, guidelines, precautions, and practices are adhered to
accordingly.
Infectious, toxic, pathogenic, and radioactive substances may only be
used in special, certified containment systems with a bio-seal in order
to prevent the material from being released.
Take suitable precautions for your own safety if there is a risk of toxic,
DANGER radioactive, or pathogenic contamination
Materials that chemically react with each other with a high level of
energy are prohibited.
Keep informed about local measures to avoid harmful emissions
(depending on the substances to be centrifuged).
Protective clothing is not required for the operation of the centrifuge.
The materials to be centrifuged may, however, require special safety
WARNING measures (e.g. centrifugation of infectious, toxic, radioactive, or
pathogenic substances).
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3 Safety
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3.6.5 Safety instructions for centrifugation


For safe operation, observe the following before starting the centrifuge:
Ensure that the centrifuge was set up properly (see chapter 5 - "Set-up
and connection").
Maintain a safety distance of at least 30 cm (12 inches) around the
centrifuge.
WARNING Do not store any dangerous goods in the centrifuge area.
Do not stay in the safety area longer than what is absolutely necessary
for the operation of the centrifuge.
Only use the centrifuge with rotors and accessories that have been
approved by the manufacturer. We explicitly warn against the use of
equipment of poor quality. Breaking glass or bursting vessels can
cause dangerous imbalances at high speeds
Ensure that rotor and buckets are correctly fitted (see chapter 6.2.2.1 -
"Installation of the rotor").
Observe the instructions on the installation of accessories (see chapter
6.2.2.3 - "Installation of accessories").
The rotor must be loaded axial symmetrically at equal weights.
If liquids with a density > 1.2 g/cm3 are used, reduce the speed (see
chapter 2.2.2.2 - "Density").
Do not use the centrifuge if the rotor is loaded asymmetrically.
WARNING Do not use the centrifuge with tubes that are excessively long.
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3.6.6 Resistance of plastics


Chemical influences have a strong effect on the polymeric chains of
plastics, and, therefore, on their physical properties. Plastic parts can be
damaged if solvents, acids, or alkaline solutions are used.

Refer to the resistance data (see chapter 11.4 - "Resistance data")!

NOTE
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3 Safety
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3.6.7 Service life of rotors and accessories


The rotors and accessories have a limited service life.
Perform regular checks (at least once per month) for safety reasons!
Pay special attention to changes, such as corrosion, cracks, material
abrasion, etc.
After 10 years, they must be inspected by the manufacturer.
WARNING After 50,000 cycles, the rotor must be scrapped for reasons of safety.
If other data concerning the service life are engraved on the rotor or
bucket, these data shall apply accordingly. For example, a bucket with
the engraving max. cycles = 10,000 has a service life of 10,000
cycles, and a rotor with the engraving Exp. date 02/20 must be
scrapped in February 2020 at the latest (see figure).

Fig. 2: Different service life engraving on the bucket/rotor

Refer to the table of rotors and accessories with a different service


life (see chapter 11.3 - "Table of the service life of rotors and
accessories")!
NOTE
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3.7 Safety devices


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3.7.1 Lid lock device


The centrifuge can only be started when the lid is properly closed. The
electrical lock must be locked. The lid can only be opened when the rotor
has stopped. If the lid is opened by way of the emergency release system
during operation, the centrifuge will immediately switch off and decelerate
brakeless. If the lid is open, the drive is completely separated from the
mains power supply, i.e. the centrifuge cannot be started (see chapter 7.1.1
- "Emergency lid release").
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3.7.2 System check


An internal system check monitors the data transfer and sensor signals with
regard to plausibility. Malfunctions are indicated by error messages with a
number in the speed/rcf display (see chapter 7.2 - "Table of error codes").
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3.8 Measures in the event of hazards and accidents

If an emergency arises, switch off the centrifuge immediately!


If in doubt, call the emergency doctor!
DANGER
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3.9 Remaining hazards


The unit was built state- of- the- art and according to the accepted safety
rules. However, danger to life and limb of the operator, or of third parties, or
impairments of the unit or other material assets cannot be completely
excluded when the unit is being used.
Use the unit only for the purpose that it was originally intended for (see
chapter 1.2 - "Intended use").
Use the unit only if it is in a perfect running state.
Immediately eliminate any problems that can affect safety.
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4 Storage and transport


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4 Storage and transport


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4.1 Dimensions and weight


Sigma 1-14

Height: 176 mm

Height with open lid: 310 mm

Width: 212 mm

Depth: 266 mm

Weight: 6 kg
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4.2 Storage conditions


The centrifuge can be stored in its original packaging for up to a year.
Store the centrifuge only in dry rooms.
The permissible storage temperature is between -20C and +60C.
If you would like to store it for more than one year, or if you intend to
ship it overseas, please contact the manufacturer.
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4.3 Notes on transport


The centrifuge is not equipped with a transport safety device.
When lifting the centrifuge, always reach under the centrifuge from the
side.

The centrifuge weighs approx. 6 kg!

CAUTION

For transport use suitable packaging and, if at all possible, the original
packaging. (see chapter 4.4 - "Packaging").
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4.4 Packaging
The centrifuge is packaged in a cardboard box.
Open the box.
Take out the box containing the accessories.
Lift the centrifuge with both foam cushions out of the cardboard box.
When lifting the centrifuge, always reach under the centrifuge from the
side.

The centrifuge weighs approx. 6 kg!

CAUTION

Retain the packaging for any possible future transport of the centrifuge.
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4.5 Transport safety device


The centrifuge is not equipped with a transport safety device.
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5 Set-up and connection


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5 Set-up and connection


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5.1 Installation site


Operate the centrifuge only in closed and dry rooms.
All the energy supplied to the centrifuge is converted into heat and emitted
to the ambient air.
Ensure sufficient ventilation.
Keep a safety distance of at least 30 cm around the centrifuge so that
the vents in the centrifuge remain fully effective.
Do not subject the centrifuge to thermal stress, e.g. by positioning it
near heat generators.
Avoid direct sunlight (UV radiation).
The table must be stable and have a solid, even surface.
Attention: During transport from cold to warmer places, condensational
water will collect inside the centrifuge. It is important to allow sufficient
time for drying (min. 24 h) before the centrifuge can be used again.
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5.2 Power supply


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5.2.1 Type of connection

The operating voltage on the name plate must correspond to the local
supply voltage!
DANGER

Sigma centrifuges are units of protection class I. The centrifuges of this


model series have a three-wire power cord with an IEC C13 connector.
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5.2.2 Customer-provided fuses


Typically, the centrifuge must be protected with 16 Amp L or B fuses that
are to be provided by the customer.
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6 Using the centrifuge


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6.1 Initial start-up

Before the initial start-up, please ensure that your centrifuge is properly
set up and installed (see chapter 5 - "Set-up and connection").

DANGER
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6.2 Switching the centrifuge on


Press the mains power switch.
The display then illuminates. The centrifuge is ready for operation.
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6.2.1 Opening and closing the lid


The lid can be opened if the centrifuge is at a standstill.
Press the lid key in order to open the lid.
The centrifuge cannot be started if the lid is opened.
To close, press with both hands slightly on the lid until the lid lock is
locked.

Do not place your fingers between the lid and the housing when closing
the lid. Risk of crushing!

WARNING
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6.2.2 Installation of rotors and accessories


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6.2.2.1 Installation of the rotor

1 Safety lock
2 Snap-on lid
3 Rotor fastening nut
4 Motor shaft
5 Angle rotor

Fig. 3: Installation of rotor and rotor lid

Open the centrifuge lid by pressing the lid key.


Unscrew the rotor fastening nut from the motor shaft (counter-
clockwise).
Lower the rotor with its central bore straight down onto the motor shaft.
Hand-tighten the rotor fastening nut clockwise (corresponds to 1 Nm).
The rotor fastening nut and conical spring washer must face the rotor
(i.e. the writing must be shown on top).

1 Conical spring washer

Fig. 4: Rotor fastening nut from above (A) and from below (B)

To lock the snap-on lid, pull the safety lock in the middle of the lock
upwards and put the lid on the rotor. The snap-on lid will be locked
when the safety lock is released.

Once a day or after 20 cycles, the rotor tie-down screw must be loosened
by some turns, and the rotor must be lifted and fastened again. This
ensures a proper connection between the rotor and the motor shaft.
WARNING

Rotors can be used without a cover. This leads, however, to higher levels
of noise and temperatures when running.
NOTE

Follow the safety instructions and hazard warnings (see chapter 3 -


"Safety")!
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6.2.2.2 Installation of the microhaematocrit rotor

Fig. 5: Microhaematokrit rotor (part no. 11026) with reader (part no. 17028)

Unscrew the rotor fastening nut from the motor shaft (anti-clockwise).
Lower the microhaematocrit rotor with its central bore straight down
onto the motor shaft.
Hand-tighten the rotor fastening nut clockwise (corresponds to 1 Nm).
The rotor fastening nut and conical spring washer must face the
microhematocrite rotor (i.e. the writing must be shown on top).

1 Conical spring washer

Fig. 6: Rotor fastening nut from above (A) and from below (B)

Follow the safety instructions and hazard warnings (see chapter 3 -


"Safety")!

Operation
Fill the capillary tubes with blood and seal them at one end with putty or
by fusion.
Place the capillary tubes into the recesses of the rotor with the sealed
end against the rubber ring. Ensure that the capillary tubes fit tightly
against the rubber ring. The opposite places must be loaded.
Put the rotor cover on and lock it.
Close the centrifuge lid.
Enter the following parameters: speed 13,000 rpm, gravitational field
RCF max. 11,903 x g, time approx. 5 min.
Start the centrifuge.
Open the centrifuge lid when the rotor has stopped.
Unlock the rotor cover and lift it off.

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Evaluation
Put the reader onto the rotor.
Turn the reader and perform a fine adjustment with the aid of the central
eccentric mechanism in order to localize the O-point and the maximum
liquid point in the capillary tubes. You can now read the percentage
value.
Remove the capillary tubes. Some of the tubes can also be evaluated
with the reader outside of the rotor (see the instructions for use on the
back of the reader).
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6.2.2.3 Installation of accessories


Only use tubes that are suitable for the rotor (see chapter 11.1 -
"Range of accessories").
Always load the axial symmetrical inserts of the rotors with the same
accessories and fill to avoid imbalance.

Centrifugation with low capacity


Install the tubes axial symmetrically so that the rotor is loaded evenly.
It is not permissible to load angle rotors on only one axis.

correct incorrect

Fig. 7: Permissible and impermissible loading of an angle rotor (example illustration)


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6.2.2.4 Tubes
Load the tubes outside of the centrifuge. Liquids in the bores of the rotor
cause corrosion.
Fill the tubes carefully and arrange them according to their weight.
Imbalances result in the excessive wear of the bearings.
Follow the safety instructions and hazard warnings (see chapter 3 -
"Safety")!
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6.3 Control System "Spincontrol Basic"


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6.3.1 User interface

1 Display
2 Select key
3 Arrow keys
4 Lid key
5 Start/Stop key

Fig. 8: User interface

The centrifuge is started directly via the user interface. When the centrifuge
is switched on, all segments will be illuminated for a short time. It is now
ready for operation.
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6.3.2 Display
The centrifuge display has the following display fields:

1 Speed / RCF field


2 Field for
acceleration /
deceleration curves
and run mode
3 Time field

Fig. 9: Display, completely active


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6.3.3 Starting a centrifugation run


The centrifuge is ready for operation when the mains power switch is on
and the lid is closed.
Press the start/stop key in order to start a centrifugation run.
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6.3.4 Interrupting a centrifugation run


Press the start/stop key in order to interrupt a centrifugation run. The
centrifugation run will be terminated prematurely.
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6.3.5 Interrupting a deceleration process


Press the start/stop key during a deceleration process in order to
interrupt it and to restart the centrifuge.
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6.3.6 Speed / Relative centrifugal force (RCF)


The RCF value is determined by the rotor geometry and speed. The RCF
and speed values, therefore, depend on each other. If one of the two
values is entered, the other value will be set automatically.
To preselect a speed value or a RCF value, press the select key
repeatedly until the corresponding unit flashes on the display.
Select the desired speed or RCF value via the arrow keys.
Confirm the selection by pressing the start/stop key.
The data will also be accepted and saved after approx. 20 seconds if no
other key is pressed within this time period.
During operation, you can switch from the speed value to the RCF value
and vice versa via the arrow keys.
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6.3.6.1 Changing the speed/RCF value during centrifugation


The preselected speed or RCF value can be changed during centrifugation.
Press the select key repeatedly until the desired unit flashes on the
display.
Change the speed or RCF value by pressing the arrow keys. The
parameters will take effect immediately.
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6.3.7 Runtime
The preselected total runtime is displayed in the lower line of the display.
During centrifugation, the remaining runtime is displayed. The runtime of
the centrifuge can be set at one-second-intervals up to 99 minutes and 59
seconds.
To select a runtime, press the select key repeatedly until the time unit
flashes on the display.
Select the desired runtime by pressing the arrow keys.
Confirm the selection by pressing the start/stop key.
The data will also be accepted and saved after approx. 20 seconds if no
other key is pressed within this time period.
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6.3.7.1 Changing the runtime during centrifugation


The preselected runtime can be changed during centrifugation.
Press the select key repeatedly until the time unit flashes on the display.
Change the desired runtime by pressing the arrow keys. The
parameters will be accepted immediately.

If the centrifugation time is changed during the run, the centrifuge will run
for the entire new time and will disregard the previous runtime that has
already elapsed.
NOTE
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6.3.7.2 Short run (quick run)


During the short run, the centrifuge accelerates at maximum power until the
maximum speed is reached.
Keep the start/stop key pressed during the short run.
The message "short run" and the duration of the short run is displayed.
When the start/stop key is released, the centrifuge decelerates at maximum
power to a standstill.
After the short run, the lid unlocks automatically and the program that was
set beforehand is displayed again.
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6.3.7.3 Continuous run


During the continuous run, the runtime of the centrifuge is unlimited and
must be stopped manually. The centrifuge accelerates during the
continuous run until the set speed is reached.
To start the continuous run, press the select key until the time unit
flashes on the display.
Press the down-key (arrow key) until the display switches from "00:10"
to "--:--".
After 99 min 59 sec, any additional runtime will no longer be displayed, but
the centrifugation will continue.
To stop a continuous run, press the start/stop key. The centrifugation
will end.
Enter a runtime. The centrifugation will end after this time.
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6.3.8 Softstart and softstop function


The softstart function is used to extend the acceleration time, whereas the
softstop function is used to extend the deceleration time. The current
combination is shown on the display.
To activate the softstart and softstop functions, press the select key
repeatedly until "Soft-stop/start" flashes in the upper left part of the
display.
Press the arrow keys until "Soft-stop/start on" is displayed. The softstart
and softstop functions are now activated.
Press the select key until "Soft stop on" is displayed. Only the softstop
function will be active and the centrifuge will start at normal speed.
Press the select key until "Soft off" is displayed. The softstart and
softstop functions are now deactivated.
The data will be accepted immediately and saved after approx. 20 seconds.
You can also change the settings as described above during a
centrifugation run.
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6.3.9 Rotor selection


In the delivery status of the centrifuge, the rotor 12094 is preselected. If
another rotor is installed the configuration must be changed, so that the
allowed maximum speed of the rotor can be reached.
To select another rotor, press the "select" key and hold it.
Press the upper arrow key three times until the rotor selection menu is
displayed, then release the select key.
Choose the number of the installed rotor out of the following list, by
pressing the arrow keys:
Code 1 2 3 4 5

Rotor 11026 12082 12083 12084 12085

Code 6 7 8 9 10

Rotor 12092 12093 12094 12096 --

Press the select key to confirm the input.


The RCF value will be adjusted automatically to the selected rotor.

The rotor selection will be saved under the corresponding program


number.

NOTE
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6.4 Switching the centrifuge off


Open the centrifuge when it is not in use so moisture can evaporate.
Switch the centrifuge off by pressing the mains power switch.
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7 Malfunctions and error correction


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7.1 General malfunctions


Malfunctions are indicated by error messages with a number in the
speed/RCF display.
In the event of a fatal error (e.g. a defective lid lock), a certain safety time
will be counted down on the display. During this time, ERR and SAFE
flash alternately on the display. When the time is up, "OFF" will be
displayed.

Do not switch the centrifuge off until OFF is displayed! This is necessary
in order to ensure that the rotor is at a complete standstill.
WARNING

Eliminate the source of the problem (see tables below).


Acknowledge the error messages with the lid key.

Type of error Possible reason Correction

No indication on the display No power in the mains supply Check fuse in the mains supply

Power cord is not plugged in Plug in power cord correctly

Mains power switch off Switch mains power switch on

Lid is not closed correctly Close the lid

Centrifuge cannot be started: The set Several Netz aus/ein. Falls sich der Fehler
speed value is displayed in an wiederholt, Service verstndigen
unchanged manner
The lid lock is not closed correctly Open and close lid. If the error
occurs again, contact service

Centrifuge decelerates during Several Power off/on. If the error occurs


operation and displays an error from 1 again, contact service
to 18 after powering on

Centrifuge decelerates during Several Quit by pressing the lid key


operation and displays error 19 after
powering on

Temperature value cannot be reached Condenser dirty Contact service


(only for refrigerated centrifuges)
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7.1.1 Emergency lid release


In the event of a power failure, it is possible to manually open the
centrifuge lid with a socket spanner (part no. 930 014, included in the scope
of supply). The opening is located in the base plate in the front quarter on
the left-hand side.
Switch the centrifuge off by actuating the mains power switch and by
unplugging the mains power plug.
Pull the centrifuge a bit over the edge of the table and jack it up (e.g. on
two wooden logs). Ensure that the centre of gravity of the centrifuge is
still located above the table top. If necessary, the unit must be secured
by a second person.
Insert the spanner for the emergency release into the hole (see the
illustration, item 1) and attach it to the shaft of the lid lock motor.
Turn the shaft carefully clockwise with the aid of the wrench until the lid
opens.
Remove the spanner and pull the centrifuge back on the table.

Fig. :10 Opening for the emergency lid release at the bottom of the centrifuge

Do not unlock the lid unless the rotor is at a standstill and unless there is
no danger to other persons!
WARNING

If the lid is opened via the emergency lid release during a centrifuge run,
the centrifuge will be switched off immediately and decelerate in a brake-
less manner.
As a general rule, the lock can be opened more easily with the emergency
lid release system if the load on it is relieved by exerting slight pressure on
the lid.
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7.2 Table of error codes


Error no. Kind of error Measures Note

1-9 System error Allow to slow down All these errors stop the
Power off/on centrifuge or cause it to
decelerate brakeless

10-19 Speedometer error Allow to slow down


Power off/on

20-29 Motor error Power off


Ensure ventilation

30-39 EEPROM error Allow to slow down With error 34, 35, and 36, the
Power off/on centrifuge will stop;
with error 37 and 38 only an
error message will be given

40-45 Temperature error Allow to slow down


(only for refrigerated Power off
centrifuges)
Allow to cool down
Provide better ventilation (only air cooled
centrifuges)
Provide sufficient water throughput (only
water cooled centrifuges)

46-49 Imbalance error Allow to slow down


Power off
Eliminate the imbalance

50-59 Lid error Press lid key With error 50 and 51, the
Close lid centrifuge will stop
Remove foreign matter from the opening
of the lid lock device

60-69 Process error Allow to slow down With error 60 message


Power off/on "power failure during run",
with error 61, the message
"stop after power on"

70-79 Communication error Allow to slow down


Power off/on

80-89 Parameter error Power off With error 83, error message
Allow to cool down only
Provide for better ventilation

90-99 Other errors Check connections


Provide sufficient water throughput (only
water cooled centrifuges)

If it is impossible to eliminate the errors, contact the service!

NOTE
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7.3 Service contact


In the event of queries, malfunctions, or spare part enquiries:

From Germany:
Contact

Sigma Laborzentrifugen GmbH


An der Unteren Sse 50
37520 Osterode (Germany)
Tel. +49 (0) 55 22 / 50 07-44 44
E-mail: support.lab@sigma-zentrifugen.de

Outside Germany:
Contact our agency in your country. All agencies are listed at
www.sigma-zentrifugen.de [Sales Partners]

If you would like to utilise our service, please state the type of your
centrifuge and its serial number.
NOTE
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8 Maintenance and service


The centrifuge, rotor, and accessories are subject to high mechanical
stress. Thorough maintenance performed by the user extends the service
life and prevents premature failure.

If corrosion or other damage occurs due to improper care, the


manufacturer cannot be held liable or subject to any warranty claims.

CAUTION

Use soap water or other water-soluble, mild cleaning agents with a pH


value between 6 and 8 for cleaning the centrifuge and accessories (see
also ).
Avoid corrosive and aggressive substances.
Do not use solvents.
Do not use agents with abrasive particles.
Do not expose the centrifuge and rotors to intensive UV radiation or
thermal stress (e.g. by heat generators).
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8.1 Maintenance
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8.1.1 Centrifuge
Unplug the mains power plug before cleaning.
Carefully remove all liquids, including water and particularly all the
solvents, acids, and alkaline solutions from the rotor chamber using a
cloth in order to avoid damage to the motor bearings.
If the centrifuge has been contaminated with toxic, radioactive, or
pathogenic substances, clean the rotor chamber immediately with a
suitable decontamination agent (depending on the type of
contamination).

Take suitable precautions for your own safety if there is a risk of toxic,
radioactive, or pathogenic contamination.

WARNING

Grease the motor shaft slightly after cleaning (grease for load-bearing
bolts part no. 70284).
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8.1.2 Accessories

For the care of the accessories, special safety measures must be


considered as these are measures that will ensure operational safety at
the same time!
CAUTION

Immediately rinse off the rotor, buckets, or accessories under running


water if they have come into contact with any liquids that may cause
corrosion. Use a brush for test tubes in order to clean the bores of angle
rotors. Turn the rotor upside down and allow it to dry completely.
Clean the accessories outside the centrifuge once a week or preferably
after each use. Adapters should be removed, cleaned and dried.

Do not clean the accessories in a dishwasher!


Cleaning in a dishwasher removes the anodised coating; the result is
cracking in areas that are subject to stress.
CAUTION

If the rotors or accessories have been contaminated with toxic,


radioactive, or pathogenic substances, clean them immediately with a
suitable decontamination agent (depending on the type of
contamination). Take suitable precautions for your own safety if there is
a risk of toxic, radioactive, or pathogenic contamination.
Dry the accessories with a soft cloth or in a drying chamber at
approx. 50C.
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8.1.2.1 Plastic accessories


The chemical resistance of plastic decreases with rising temperatures (see
chapter 11.4 - "Resistance data").
If solvents, acids, or alkaline solutions have been used, clean the plastic
accessories thoroughly.

Plastic accessories must not be greased!

WARNING
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8.1.3 Rotors
The rotors are produced with the highest precision, in order to withstand the
permanent high stress from high gravitational fields.
Chemical reactions as well as stress-corrosion (combination of oscillating
pressure and chemical reaction) can affect or destroy the metals. Barely
detectable cracks on the surface can expand and weaken the material
without any visible signs.
Check the material regularly (at least once a month) for
cracks
visible damage of the surface
pressure marks
signs of corrosion
other changes.

Check the bores of the rotors and multiple carriers.


Replace any damaged components immediately for your own safety.
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8.1.4 Microhaematocrit rotor


Remove the microhaematrocrit rotor for cleaning.
Wipe the centrifuge chamber clean.
Replace the rubber ring in the event of wear or glass breakage (part no.
16002 for rotor 11026).
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8.1.5 Glass breakage

In the case of glass breakage, immediately remove all glass particles (e.g.
with a vacuum cleaner). Replace the rubber cushions since even thorough
cleaning will not remove all glass particles.
CAUTION
Glass particles will damage the surface coating (e.g. anodising) of the
buckets, which will then lead to corrosion.
Glass particles in the rubber cushions of the buckets will cause glass
breakage again.
Glass particles on the pivot bearing of the load- bearing bolts prevent the
buckets and carriers from swinging evenly, which will cause an imbalance.
Glass particles in the rotor chamber will cause metal abrasion due to the
strong air circulation. This metal dust will not only pollute the rotor chamber,
rotor, and materials to be centrifuged but also damage the surfaces of the
accessories, rotors, and rotor chamber.

In order to completely remove the glass particles and metal dust from
the rotor chamber:
Grease the upper third of the rotor chamber with e.g. Vaseline.
Then, let the rotor rotate for a few minutes at a moderate speed
(approx. 2000 rpm). The glass and metal particles will now collect at the
greased part.
Remove the grease with the glass and metal particles with a cloth.
If necessary, repeat this procedure.
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8.2 Sterilisation and disinfection of the rotor chamber and


accessories
Use commercially-available disinfectants such as, for example,
Sagrotan, Buraton, or Terralin (available at chemists shops or
drugstores).
The centrifuge and the accessories consist of various materials. A
possible incompatibility must be considered.
Before using cleaning or decontamination agents that were not
recommended by us, contact the manufacturer to ensure that such a
procedure will not damage the centrifuge.
For autoclaving, consider the continuous heat resistance of the
individual materials (see chapter 8.2.1 - "Autoclaving").
Please contact us if you have any queries (see chapter 7.3 - "Service
contact").

If dangerous materials (e.g. infectious and pathogenic substances) are


used, the centrifuge and accessories must be disinfected.

DANGER
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8.2.1 Autoclaving
The service life of the accessories essentially depends on the frequency of
autoclaving and use.
Replace the accessories immediately when the parts show changes in
colour or structure or in the occurrence of leaks etc.
During autoclaving, the caps of the tubes must not be screwed on in
order to avoid the deformation of the tubes.

It cannot be excluded that plastic parts, e.g. lids or carriers, may deform
during autoclaving.
NOTE

Accessories Max. temp. Min. time Max. time Max. cycles


(C) (min) (min)

Aluminium buckets 134-138 3 5 -

Aluminium rotors 134-138 3 5 -

Glass tubes 134-138 3 40 -

Polyallomer / polycarbonate rectangular carriers 115-118 30 40 -

Polyallomer / polycarbonate round carriers 115-118 30 40 -

Polyamide buckets 115-118 30 40 10

Polycarbonate / polyallomer lids for angle rotors 115-118 30 40 20

Polycarbonate tubes 115-118 30 40 20

Polyphenylsulfone (PPSU) caps for buckets 134-138 3 5 100

Polypropylene balance adapter for blood-bag systems 115-118 30 40 n.s.

Polypropylene copolymer tubes 115-121 30 40 20

Polypropylene rectangular carriers 115-118 30 40 -

Polypropylene rotors 115-118 30 40 20

Polypropylene round carriers 115-118 30 40 -

Polysulfone caps for buckets 134-138 3 5 100

Polysulfone lids for angle rotors 134-138 3 5 100

Rubber adapters 115-118 30 40 -

Stainless-steel balance weight for blood-bag systems 121 30 30 n.s.

Teflon tubes 134-138 3 5 100


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8.3 Service

In the event of service work that requires the removal of the panels, there
is a risk of electric shock or mechanical injury. Only qualified specialist
personnel is authorised to perform this service work.
DANGER

The centrifuge is subject to high mechanical stress. In order to be able to


withstand this high level of stress, high-quality components were used
during the production of the centrifuge. Nevertheless, wear cannot be
excluded and it may not be visible from the outside. Especially the rubber
parts that are among other things part of the motor suspension, are
subject to ageing.
This is why we recommend having the centrifuge checked by the
manufacturer during an inspection once per year in the operating state and
once every three years in the dismantled state. Rubber parts should be
replaced after three years.

Information and appointments:

In Germany:
Contact
Sigma Laborzentrifugen GmbH
An der Unteren Sse 50
37520 Osterode (Germany)
Tel. +49 (0) 55 22 / 50 07-44 44
E-mail: support.lab@sigma-zentrifugen.de

Outside Germany:
Contact our agency in your country. All agencies are listed at
www.sigma-zentrifugen.de [Sales Partners]

If you would like to utilise our service, please state the type of your
centrifuge and its serial number.

NOTE
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8.4 Return of defective parts


Although we exercise great care during the production of our products, it
may be necessary to return a unit or accessory to the manufacturer.
In order to ensure the quick and economical processing of returns of
centrifuges, spare parts, or accessories, we require complete and extensive
information concerning the process. Please fill in the following forms
completely, sign them, enclose them with the return package, and send
them together with the product to:
Sigma Laborzentrifugen GmbH
An der Unteren Sse 50
37520 Osterode (Germany)

1. Declaration of decontamination
As a certified company and due to the legal regulations for the
protection of our employees and of the environment, we are obliged to
certify the harmlessness of all incoming goods. For this purpose, we
require a declaration of decontamination.
The form must be filled in completely and signed by authorised and
specialised personnel only.
Affix the original form in a clearly visible manner to the outside of the
packaging.

We will return the unit if no declaration of decontamination is provided!

NOTE

2. Form for the return of defective parts


This form is for the product-related data. They facilitate the assignment,
and they enable the quick processing of the return. If several parts are
returned together in one packaging, please enclose a separate problem
description for every defective part.
A detailed problem description is necessary in order to perform the
repair quickly and economically.

If the form does not include a description of the malfunction, neither a


refund nor a credit note can be issued. In this case, we reserve the right to
return the parts to you at your expense.
NOTE

Upon request, we will prepare and submit to you a cost estimate


prior to performing the repair. Please confirm such cost estimate
within 14 days. If the cost estimate has still not been confirmed after
4 weeks, we will return the unit. Please note that you must bear the
incurred costs.

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The unit must be packaged in a transport-safe manner. Please use the


original packaging, if at all possible.
If the product is dispatched to us in unsuitable packaging, you will be
charged the cost for returning it to you in new packaging.
NOTE

The forms can be downloaded online from


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www.sigma-zentrifugen.de [Service] [Overhaul and repair].
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9 Disposal
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9.1 Disposal of the centrifuge


In accordance with the directive 2002/96/EC, SIGMA centrifuges are
marked with the symbol shown to the left. This symbol means that it is not
permissible to dispose of the unit among household waste.
You can return these centrifuges free of cost to
Sigma Laborzentrifugen GmbH.
Ensure that the unit is decontaminated. Fill in a declaration of
decontamination (see chapter 8.4 - "Return of defective parts").
Comply with any other applicable local rules and regulations.
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9.2 Disposal of the packaging


Use the packaging to return the centrifuge for disposal or
dispose of the packaging, after having separated the individual
materials.
Comply with all local rules and regulations.
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10 Technical data
Manufacturer Sigma Laborzentrifugen GmbH
An der Unteren Sse 50
37520 Osterode
Germany

Type: 1-14

Connection requirements
Electrical connection: see name plate
Protection class: I
IP code: 20
Connected load (kVA): 0.157
Power consumption (kW): 0.095
Max. current consumption (A): 0,68 (at 220-240 V / 50-60 Hz)
Input fuse (AT): 2.0 (at 220-240 V / 50-60 Hz)

Performance data
Max. speed (rpm): 14,800
Max. capacity (ml): 48
Max. gravitational field (x g) 16,163
Max. kinetic energy (Nm): 1,721

Other parameters
Time range: 10 sec to 99 min 59 sec, short run, continuous run

Physical data
Height (mm): 176
Height with opened lid (mm): 310
Width (mm): 212
Depth (mm): 266
Weight (kg): 6.0
Noise level (dB(A)): <60 (at max. speed)
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10.1 Ambient conditions


The figures are valid for an ambient temperature of +23C 2C and a
nominal voltage 10 %.

At a nominal voltage of 100V or 200V, a tolerance of +10% / -5% applies.

NOTE

For indoor use only.


Allowable ambient temperature +5C to +40C.
Max. relative humidity of air 80% up to 31C with a linear decrease to
50% relative humidity of air at 40C.
Maximum altitude 2,000 m above sea level.
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10.2 Technical documentation


For environmental reasons, the comprehensive technical documentation of
the centrifuge (e.g. circuit diagrams) and the safety data sheets of the
manufacturers of refrigerant and lubricants are not attached to this
documentation.
You can order these documents from our service department.
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11 Appendix
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11.1 Range of accessories


Part-no. Description Max. speed Max.
(rpm) gravitational
field (x g)

11026 Microhaematocrit rotor incl. lid, suitable for 24 capillary tubes


1.3 x 50 mm, 19 l no. 15028, incl. reader no. 17028,
usable with reader no. 17026
Radius max. 6.3 cm, radius min. 1.3 cm 13 000 11 903

12082 Angle rotor 12 x 1.5/2.0 ml, for reaction vials e.g. no. 15008, 15040,
incl. polysulfone lid no. 17882
Radius max. 6.2 cm, radius max. 2.9 cm, angle 45 14 800 15 183

12083 Angle rotor 18 x 1.5/2.0 ml, for reaction vials, e.g. no. 15008, 15040 or
18 x 0.25/0.4 ml e.g. no. 15014, 2 lines, incl. polysulfone lid no. 17882,
angle 32/57
Radii max. Radii min.
6.4 cm 3.2 cm 14 800 15 673
5.9 cm 2.5 cm 14 448

12084 Angle rotor 24 x 1.5/2.0 ml, for reaction vials, e.g. no. 15008, 15040,
incl. polysulfone lid no. 17882
Radius max. 6.6 cm, radius min. 3.8 and 3.0 cm, angle 32/37 14 800 16 163

12092 Angle rotor made of polypropylene, 12 x 1.5/2.0 ml, for reaction vials e.g.
no. 15008, 15040, incl. polysulfone lid no. 17882, angle 45
Radius max. 6.2 cm, radius min.2.9 cm 14 800 15 183

12093 Angle rotor made of polypropylene, 18 x 1.5/2.0 ml, for reaction vials
e.g. no. 15008, 15040 or 18 x 0,25/0,4 ml e.g. no. 15014, 2 lines,
incl. polysulfone lid no. 17882, 42/48
Radii max. Radii min.
6.4 cm 3.2 cm 14 800 15 673
5.9 cm 2.5 cm 14 448

12094 Angle rotor made of polypropylene, 24 x 1.5/2.0 ml, for reaction vials e.g.
no. 15008, 15040, incl. polysulfone lid no. 17882, angle 32/53
Radius max. 6.6 cm, radius min. 3.8 and 3.0 cm 14 800 16 163

12096 Angle rotor made of polypropylene, for 2 PCR-strips with 8 tubes 0.2 ml
e.g no. 15042, incl. polysulfone lid no. 17882, angle 45
Radius max. 4.8 cm, radius 2.1 cm 14 800 11 755

12097 Angle rotor 12 x 1.5/2.0 ml high edge, specially for reaction vials with filter,
incl. polysulfone lid no. 17933, angle 45
Radius max. 6.2 cm, radius 2.9 cm 14 800 15 183

13000 Adapter for reaction vials 0.25/0.4 ml no. 15014, 1 set = 2 pcs.

13002 Adapter for reaction vials 0.5/0.75 ml, Adapter for reaction vials
0.5/0.75 ml, e.g. no. 15005

13021 Adapter for PCR-tube 0.2 ml, e.g no. 15042, 1 set = 2 pcs.

The complete list of accessories can be downloaded from


www.sigma-zentrifugen.de
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11.1.1 Rotor radii


The information in the accessories table concerning the radius refers to the
values of the respective rotor as shown below. The radius calculation is
described in chapter 2.2.2.1 - "Speed, radius, and relative centrifugal
force".

Fig. 11: Minimum and maximum radius of an angle rotor

Fig. 12: Microhaematocrit rotor


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11.2 Speed-gravitational-field-diagram

Fig. 13: Speed-gravitational-field-diagram


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11.3 Table of the service life of rotors and accessories

Rotors and accessories


with a different service life
If no other data concerning the service life are engraved on the rotor or accessory, rotors and
buckets must be checked by the manufacturer after 10 years. After 50,000 cycles, rotors must be
scrapped for safety reasons.

Rotor / Cycles Service life Autoclaving Suitable for centrifuge Remarks


bucket ("Exp.Date")

without engraving, only


4-15C, 4K15C, 4-16, 4-16S,
"spincontrol
9100 15,000 4-16K, 4-16KS, 6-15, 6K15,
professional" and
6-16, 6-16K
"spincontrol S"
11026 7 years 1-14, 1-14K
12033 5 years 1-16 Edition, 1-16K Edition
12082 7 years 1-14, 1-14K
12083 7 years 1-14, 1-14K
12084 7 years 1-14, 1-14K
12085 7 years 1-14, 1-14K
12092 5 years 20x 1-14, 1-14K
12093 5 years 20x 1-14, 1-14K
12094 5 years 20x 1-14, 1-14K
12096 5 years 20x 1-14, 1-14K
12101 5 years 20x 1-15, 1-15K, 1-15P, 1-15PK
12124 5 years 20x 1-15, 1-15K, 1-15P, 1-15PK
12126 5 years 20x 1-15, 1-15K, 1-15P, 1-15PK
12134 5 years 20x 1-16, 1-16K
12135 5 years 20x 1-16, 1-16K
12137 5 years 20x 1-16, 1-16K
12500 7 years 6-15, 6K15, 6-16, 6-16K
12600 7 years 6-16S, 6-16KS
4-16, 4-16S, 4-16K, 4-16KS,
13218 20,000
6-16, 6-16S, 6-16K, 6-16KS
13296 5 years 10x 2-6, 2-6E, 2-7
2-6, 2-6E, 2-7,
13299 5 years 10x
2-16P, 2-16KL, 2-16KHL
13635 25,000 6-16, 6-16K, 6-16S, 6-16KS
13845 20,000 8K, 8KS
13850 10,000 8K, 8KS, 8KBS
13860 35,000 8K, 8KS, 8KBS
13864 1,000 8K, 8KS without engraving
13865 1,000 8K, 8KS without engraving
13866 1,000 8K, 8KS without engraving

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11.4 Resistance data

The data refer to resistance at 20C.

NOTE

Polyvinyl chloride, weak


Polyvinyl chloride, hard

Acrylonitrile-butadiene-
Polytetrafluorethylene
Polyoxymethylene
- no data

Polycarbonate

Polypropylene
1 resistant Concentration

Polyethylene
High Density

Polysulfone

caoutchouc
2 practically resistant

Aluminium
Polyamide
3 partially resistant
4 not resistant HDPE

PTFE
POM

NBR
PSU

PVC

PVC
[%]

PC
PA

PP

AL
Medium Formula
Acetaldehyde C2H4O 40 3 2 4 2 3 4 4 - 1 4 1
Acetamide C2H5NO saturated 1 1 4 1 1 4 4 - 1 - 1
Acetone C3H6O 100 1 1 4 1 1 4 4 - 1 4 1
Acrylonitrile C3H3N 100 1 1 4 3 3 4 4 4 1 4 1
Allyl alcohol C3H6O 96 1 3 3 2 2 2 2 4 1 1 1
Aluminium chloride AlCl3 saturated 1 3 2 4 1 - 1 - 1 1 4
Aluminium sulfate Al2(SO4)3 10 1 1 1 3 1 1 1 1 1 1 1
Ammonium chloride (NH4)Cl aqueous 1 1 1 2 1 1 1 1 1 1 3
Ammonium hydroxide NH3 + H2O 30 1 3 4 1 1 2 1 - 1 - 1
Aniline C6H7N 100 1 3 4 1 2 4 4 4 1 4 1
Anisole C7H8O 100 3 4 4 1 4 4 2 - 1 4 1
Antimony trichloride SbCl3 90 1 4 1 4 1 - 1 - 1 - 4
Benzaldehyde C7H6O 100 1 3 4 1 1 3 4 4 1 4 1
Benzene C6H6 100 3 2 4 1 3 4 4 - 1 4 1
Boric acid H3BO3 aqueous 1 3 1 2 1 - - - 1 1 1
Butyl acrylate C7H12O2 100 1 2 4 2 3 4 4 4 1 - 1
Butyl alcohol, normal C4H10O 100 1 1 2 1 1 2 2 4 1 1 1
Calcium chloride CaCl2 alcoholic 1 4 2 3 1 - - 4 1 1 3
Carbon disulfide CS2 100 4 3 4 2 4 4 4 4 1 3 1
Carbon tetrachloride
CCl4 100 4 4 4 2 4 4 4 4 1 3 1
(TETRA)
Chlorine Cl2 100 4 4 4 4 4 4 4 4 1 - 3
Chlorine water Cl2 x H2O 3 4 4 4 3 - 3 3 1 - 4
Chlorobenzene C6H5Cl 100 3 4 4 1 3 4 4 4 1 4 1
Chloroform CHCl3 100 3 3 4 4 3 4 4 4 1 4 3

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Polyvinyl chloride, weak


Polyvinyl chloride, hard

Acrylonitrile-butadiene-
Polytetrafluorethylene
Polyoxymethylene
- no data

Polycarbonate

Polypropylene
Concentration
1 resistant

Polyethylene
High Density

Polysulfone

caoutchouc
2 practically resistant

Aluminium
Polyamide
3 partially resistant
4 not resistant

HDPE

PTFE
POM

NBR
PSU

PVC

PVC
[%]

PC
PA

PP

AL
Medium Formula
Chromic acid CrO3 10 1 4 2 4 1 4 1 - 1 4 1
Chromic potassium KCr(SO4)2 x
saturated 1 2 1 3 1 - 1 - 1 - 3
sulphate 12H2O
Citric acid C6H8O7 10 1 1 1 2 1 1 1 1 1 1 1
Citric acid C6H8O7 50 1 3 1 2 1 - - - 1 1 1
Copper sulphate CuSO4 x 5H2O 10 1 1 1 1 1 1 1 1 1 1 4
Cyclohexanol C6H12O 100 1 1 3 1 1 1 1 4 1 2 1
Decane C10H22 100 - 1 2 1 3 - - - 1 2 1
Diaminoethane C2H8N2 100 1 1 3 1 1 - 3 4 1 1 1
Diesel fuel 100 1 1 3 1 1 - 1 3 1 1 1
Dimethyl formamide
C3D7NO 100 1 1 4 1 1 4 3 - 1 3 1
(DMF)
Dimethyl sulfoxide
C2H6SO 100 1 2 4 1 1 4 4 - 1 - 1
(DMSO)
Dimethylaniline C8H11N 100 - 3 4 2 4 - - - 1 - 1
Dioxane C4H8O2 100 2 1 4 1 3 2 3 4 1 3 1
Dipropylene glycol
C4H10O 100 3 1 4 1 4 4 4 4 1 - 1
(mono)methyl ether
Ethyl acetate C4H8O2 100 1 1 4 1 1 4 4 4 1 4 1
Ethylene chloride C2H4Cl2 100 3 3 4 1 3 4 4 4 1 - 1
Ferrous chloride FeCl2 saturated 1 3 1 3 1 1 1 1 1 - 4
Formaldehyde solution CH2O 30 1 3 1 1 1 - - - 1 2 1
Formic acid CH2O2 100 1 4 3 4 1 3 3 1 1 2 1
Furfural C5H4O2 100 1 3 3 2 4 - - - 1 4 1
Gasoline C5H12 - C12H26 100 2 1 3 1 3 3 2 - 1 1 1
Glycerol C3H8O3 100 1 1 3 1 1 1 1 2 1 1 1
Heptane, normal C7H16 100 2 1 1 1 2 1 2 4 1 1 1
Hexane, n- C6H14 100 2 1 2 1 2 1 2 4 1 1 1
Hydrogen chloride HCl 5 1 4 1 4 1 1 1 - 1 2 4
Hydrogen chloride HCl concentrated 1 4 4 4 1 1 2 3 1 4 4
Hydrogen peroxide H2O2 3 1 3 1 1 1 1 1 - 1 3 3
Hydrogen peroxide H2O2 30 1 4 1 4 1 1 1 - 1 3 3
Hydrogen sulphide H2S 10 1 1 1 1 1 1 1 3 1 3 1
Iodine, tincture of I2 1 4 3 1 1 - 4 4 1 1 1

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Polyvinyl chloride, weak


Polyvinyl chloride, hard

Acrylonitrile-butadiene-
Polytetrafluorethylene
Polyoxymethylene
- no data

Polycarbonate

Polypropylene
Concentration
1 resistant

Polyethylene
High Density

Polysulfone

caoutchouc
2 practically resistant

Aluminium
Polyamide
3 partially resistant
4 not resistant

HDPE

PTFE
POM

NBR
PSU

PVC

PVC
[%]

PC
PA

PP

AL
Medium Formula
Isopropyl alcohol C3H8O 100 1 1 1 1 1 1 1 4 1 - 2
Lactic acid C3H6O3 3 1 3 1 2 1 1 2 - 1 1 1
Magnesium chloride MgCl2 10 1 1 1 1 1 1 1 1 1 1 1
Mercuric chloride HgCl2 10 1 4 1 3 1 1 1 1 1 1 4
Mercury Hg 100 1 1 1 1 1 1 1 3 1 1 3
Methyl acetate C3H6O2 100 1 1 4 2 1 - 4 4 1 - 1
Methyl alcohol CH4O 100 1 2 4 1 1 3 1 3 1 2 1
Methyl benzene C7H8 100 3 1 4 1 3 4 4 4 1 4 1
Methyl ethyl ketone
C4H8O 100 1 1 4 1 1 4 4 4 1 4 1
(MEK)
Methylene chloride CH2Cl2 100 4 3 4 3 3 4 4 4 1 - 1
Mineral oil 100 1 1 1 1 1 1 1 - 1 1 1
Nitric acid HNO3 10 1 4 1 4 1 1 1 - 1 4 3
Nitric acid HNO3 100 4 4 4 4 4 - 4 - 1 4 1
Nitrobenzene C6H5NO2 100 3 4 4 3 2 4 4 4 1 4 1
Oleic acid C18H34O2 100 1 1 1 2 1 - 1 - 1 3 1
Oxalic acid C2H2O4 x 2H2O 100 1 3 1 4 1 1 1 1 1 2 1
Ozone O3 100 3 4 1 4 3 1 1 - 1 4 2
Petroleum 100 1 1 3 1 1 1 1 3 1 1 1
Phenol C6H6O 10 1 4 4 4 1 4 1 3 1 3 1
Phenol C6H6O 100 2 4 4 4 1 3 4 3 1 3 1
Phosphoric acid H3PO4 20 1 4 2 4 1 - - - 1 2 4
Phosphorus
PCl5 100 - 4 4 4 1 - 4 4 1 - 1
pentachloride
Potassium hydrogen
CHKO3 saturated 1 1 2 1 1 - - - 1 - 4
carbonate
Potassium hydroxide KOH 30 1 1 4 3 1 1 1 1 1 - 4
Potassium hydroxide KOH 50 1 1 4 3 1 1 1 1 1 - 4
Potassium nitrate KNO3 10 1 1 1 1 1 - - - 1 1 1
Potassium
KMnO4 100 1 4 1 1 1 - 1 - 1 3 1
permanganate
Pyridine C5H5N 100 1 1 4 1 3 4 4 4 1 4 1
Resorcinol C6H6O2 5 1 4 2 3 1 4 2 - 1 - 2
Silver nitrate AgNO3 100 1 1 1 1 1 1 1 1 1 2 4

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11 Appendix

Polyvinyl chloride, weak


Polyvinyl chloride, hard

Acrylonitrile-butadiene-
Polytetrafluorethylene
Polyoxymethylene
- no data

Polycarbonate

Polypropylene
Concentration
1 resistant

Polyethylene
High Density

Polysulfone

caoutchouc
2 practically resistant

Aluminium
Polyamide
3 partially resistant
4 not resistant

HDPE

PTFE
POM

NBR
PSU

PVC

PVC
[%]

PC
PA

PP

AL
Medium Formula
Sodium bisulphite NaHSO3 10 1 1 2 4 1 - - - 1 1 1
Sodium carbonate Na2CO3 10 1 1 1 1 1 - - - 1 - 3
Sodium chloride NaCl 30 1 1 1 1 1 1 1 1 1 1 3
Sodium hydroxide NaOH 30 1 1 4 1 1 1 1 1 1 2 4
Sodium hydroxide NaOH 50 1 1 4 1 1 1 1 - 1 2 4
Sodium sulfate Na2SO4 10 1 1 1 1 1 1 1 1 1 1 1
Spirits C2H6O 96 1 1 1 1 1 1 1 3 1 - 1
Styrene C8H8 100 4 1 4 1 3 - 4 4 1 4 1
Sulphuric acid H2SO4 6 1 4 1 4 1 1 1 - 1 2 3
Sulphuric acid H2SO4 fuming 4 4 4 4 4 4 4 4 1 4 3
Tallow 100 1 1 1 1 1 - 1 1 1 1 1
Tetrahydrofuran (THF) C4H8O 100 3 1 4 1 3 4 4 4 1 3 1
Tetrahydronaphthalene C10H12 100 3 1 4 1 4 4 4 4 1 - 1
Thionyl chloride Cl2SO 100 4 4 4 2 4 4 4 4 1 - 3
Tin chloride SnCl2 10 1 4 2 2 1 - - - 1 1 4
Transformer oil 100 1 1 3 3 1 1 1 - 1 1 1
Trichloroethane C2H3Cl3 100 3 3 4 2 4 4 4 4 1 4 4
Urea CH4N2O 10 1 1 1 1 1 - - - 1 1 1
Urine 100 1 1 1 1 1 - 1 1 1 - 2
Vinegar C2H4O2 10 1 4 1 1 1 1 1 1 1 2 1
Vinegar C2H4O2 90 1 4 4 4 1 3 1 4 1 - 1
Wax 100 - 1 1 1 - - - 1 - 1
Wines 100 1 1 1 2 1 1 1 1 1 - 4
Xylene C8H10 100 3 1 4 1 4 4 4 4 1 4 1
Pos : 196 /010 Uni vers almodul e/Seitenwechs el @ 0\mod_1202116244312_0.doc x @ 105 @ @ 1

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Pos : 197 /100 Sig ma/110 BA Z entrifugen Sig ma (Proj ekte)/1-14_1- 14K/110 Anhang/110- 0060 Konfor mitts er klr ung 1-14 @ 28\mod_1405320897370_68.doc x @ 195755 @ 2 @ 1

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11 Appendix

11.5 EC Declaration of Conformity

=== Ende der Liste fr T extmar ke Inhalt ===

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Index

12 Index

A Contamination...................................... 19, 40
Continuous heat resistance ........................ 42
Acceleration time
Continuous run .......................................... 33
- see also the softstart and softstop function
............................................................ 33 Copyright ................................................... 10
Accessories, cleaning and care.................. 40 Corrosion ................ 18, 21, 29, 39, 40, 41, 42
Acid................................................ 20, 39, 41 Cost estimate ............................................. 45
Adapters .................................................... 40 Cracking .................................................... 40
Alkaline solutions ........................... 20, 39, 41 Cracks ....................................................... 41
Ambient conditions..................................... 48 Customer-provided fuses ........................... 25
Ambient temperature ................................. 48 D
Anodised coating ....................................... 40 Damage of the surface ............................... 41
Application examples ................................. 13 Dangerous goods....................................... 20
Autoclaving ................................................ 43 Dangerous materials .................................. 42
C Deceleration time
Capacity ..................................................... 48 - see also the softstart and softstop function
............................................................ 33
Capillary tubes ........................................... 28
Declaration of conformity ..................... 10, 59
CE mark in compliance with the directive
2006/42/EC ............................................ 15 Declaration of decontamination .................. 45
Centrifugation of infectious, toxic, radioactive, Declaration of Decontamination ................. 47
or pathogenic substances ....................... 19 Decontamination agent ........................ 40, 42
Centrifugation principle .............................. 13 Deformation of tubes.................................. 43
Centrifugation with low capacity ................. 29 Density....................................................... 20
Centrifuge cannot be started ...................... 35 Depth ......................................................... 48
Centrifuge decelerates during operation .... 35 Different service life of rotors and accessories
Centrifuge, cleaning and care .................... 39 ......................................................... 21, 53
Centrifuges Dimensions and weight .............................. 23
- definition ................................................. 9 Direct hazard to the life and health ............. 16
Changes in colour ...................................... 43 Directive 2002/96/EC ................................. 47
Changes in structure .................................. 43 Disinfectants .............................................. 42
Changing the runtime during centrifugation 32 Disinfection of the rotor chamber and
accessories ............................................ 42
Changing the Speed/RCF Value during
Centrifugation ......................................... 31 Disposal of the centrifuge........................... 47
Chemical and biological safety ................... 19 Disposal of the packaging .......................... 47
Chemical resistance of plastic .................... 41 Documentation ........................................... 10
Cleaning agents ......................................... 42 E
Cleaning the bores of angle rotors ............. 40 EC declaration of conformity ................ 10, 59
Cleaning the centrifuge .............................. 39 EEPROM error ........................................... 37
Closing the lid ...................................... 18, 26 Electrical connection .................................. 48
Communication error ................................. 37 Electrical safety .......................................... 18
Condensation............................................. 25 Emergency lid release ............................... 36
Connected load .......................................... 48 Error correction .......................................... 35
Connection cable ....................................... 10 Error messages ......................................... 35

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Index
Explanation of the symbols and notes ........ 16 Lid lock is not closed correctly.................... 35
Explosive substances ................................ 19 M
F Mains power switch off ............................... 35
Fatal error .................................................. 35 Mains voltage............................................. 18
Fire preventions ......................................... 19 Maintenance .............................................. 39
Form for the return of defective parts ......... 45 Malfunction ................................................ 35
Functional and operating elements ............ 11 Malfunctions and error correction ............... 35
G Manufacturer.............................................. 48
Marking of the unit ..................................... 15
General conditions ....................................... 9
Max. capacity ............................................. 48
General malfunctions ................................. 35
Max. current consumption .......................... 48
Glass breakage .......................................... 42
Max. gravitational field ............................... 48
Glass particles ........................................... 42
Max. speed ................................................ 48
Gravitational field ................................. 28, 48
Measures in the event of hazards and
Grease for load bearing bolts ..................... 39
accidents ................................................ 22
H Mechanical safety ...................................... 18
Hazard warnings .............................. 9, 27, 29 Microhaematocrit rotor ......................... 28, 41
Height ........................................................ 48 Mode of operation ...................................... 13
Highly corrosive substances ...................... 18 Motor error ................................................. 37
I Motor shaft ..................................... 27, 28, 39
Imbalance .................................................. 29 N
Imbalance error .......................................... 37 Name plate ................................................ 12
Importance of the operating manual ............. 9 No indication on the display ....................... 35
Important information ................................. 16 No power in the mains supply .................... 35
Infectious substances ................................ 42 Noise level ................................................. 48
Inflammable substances ............................ 19 Notes on safety and hazards ....................... 9
Informal safety instructions ........................ 17 Notes on transport ..................................... 23
Initial start-up ............................................. 26 O
Inspection by the manufacturer .................. 44
Online download of forms .......................... 46
Installation of accessories .......................... 29
Opening the lid ........................................... 26
Installation of rotors and accessories ......... 27
Operating personnel .................................. 17
Installation of the microhaematocrit rotor ... 28
Operating voltage....................................... 25
Installation of the rotor ............................... 27
Operational safety ...................................... 40
Installation site ........................................... 25
Overseas shipping ..................................... 23
Intended use ................................................ 9
P
Interrupting a centrifugation run ................. 31
Interrupting a deceleration process ............ 31 Packaging .................................................. 24
IP code ...................................................... 48 Parameter .................................................. 28
Parameter error ......................................... 37
L
Pathogenic substances .................. 19, 39, 42
Layout of the centrifuge.............................. 11
Performance data ...................................... 48
Leaks ......................................................... 43
Plastic accessories, cleaning and care....... 41
Lid error ..................................................... 37
Potential hazard to the life and health ........ 16
Lid is not closed correctly ........................... 35
Potentially hazardous situation................... 16
Lid lock device ........................................... 22
Power consumption ................................... 48
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Index
Power cord is not plugged in ...................... 35 Service life ................................................. 39
Power supply ............................................. 25 Service life of rotors and accessories ... 21, 53
Pressure marks .......................................... 41 Service life of the accessories .................... 43
Prevention of accidents ................................ 9 Service work .............................................. 44
Problem description ................................... 45 Set-up and connection ............................... 25
Process error ............................................. 37 Short run .................................................... 32
Protection class ................................... 25, 48 Softstart and softstop function .................... 33
Q Solvents ......................................... 20, 39, 41
Spanner for emergency lid release ...... 10, 36
Quick run ................................................... 32
Spare part enquiries................................... 38
R
Speed ............................................ 14, 31, 48
Radioactive substances ....................... 19, 39 Speed-gravitational-field-diagram .............. 52
Radius ....................................................... 14 Speedometer error ..................................... 37
Range of accessories ................................ 50 Spincontrol Basic ....................................... 30
RCF ........................................................... 31 Standards and regulations ......................... 10
Relative centrifugal force (RCF) ........... 14, 31 Starting a centrifugation run ....................... 31
Remove glass particles and metal dust from Sterilisation of the rotor chamber and
the rotor chamber ................................... 42 accessories ............................................ 42
Responsibility of the operator ..................... 17 Storage and transport ................................ 23
Return of centrifuges, spare parts, or Storage conditions ..................................... 23
accessories ............................................ 45
Stress-corrosion
Return of defective parts ............................ 45
see
Risk of electrical shock............................... 18
corrosion ............................................. 41
Rotor chamber ........................................... 39
Structural changes ..................................... 19
Rotor fastening nut......................... 10, 27, 28
Supply voltage ........................................... 25
Rotor radii .................................................. 51
Switching the centrifuge off ........................ 34
Rotor selection ........................................... 34
System check ............................................ 22
Rotors and accessories with a different
System error .............................................. 37
service life ........................................ 21, 53
Rotors, cleaning and care .......................... 41 T
Runtime ..................................................... 32 Table of error codes ................................... 37
S Table of rotors and accessories with a
different service life .......................... 21, 53
Safety area ................................................ 20
Technical data ........................................... 48
Safety devices ........................................... 22
Technical documentation ........................... 49
Safety distance .............................. 18, 20, 25
Temperature error ...................................... 37
Safety instructions............................ 9, 27, 29
Temperature value cannot be reached....... 35
Safety instructions for centrifugation .......... 20
Thermal stress ........................................... 39
Safety, chemical and biological .................. 19
Time range................................................. 48
Safety, electrical ........................................ 18
Toxic substances ................................. 19, 39
Safety, mechanical..................................... 18
Transport safety device .............................. 24
Safety-conscious work ............................... 17
Type........................................................... 12
Scope of supply ......................................... 10
Type of connection..................................... 25
Serial number ................................ 12, 38, 44
Type of the centrifuge .................... 38, 44, 48
Service ....................................................... 44
U
Service contact .......................................... 38
Units of protection class I ........................... 25
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Index
User interface ............................................ 30 Wear .......................................................... 44
UV radiation ......................................... 25, 39 Weight ....................................................... 48
V Width ......................................................... 48
Work on the power supply system ............. 18
Vents ......................................................... 25
Y
W
Year of manufacture .................................. 12
Warranty and liability .................................... 9

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