Documenti di Didattica
Documenti di Professioni
Documenti di Cultura
CYANOCOBALAMIN CONTENT
Determine by microbiological method.
Test organism: E. Coli M200 (N.C.I.B)
Culture Maintenance medium: Himedia B12 Culture agar (M 185)
Reconstitute the dehydrated medias as per the manufacturers instruction. Adjust the H. Boil to melt the
agar. Mix thoroughly and dispense the medium in 15 18ml amounts in screw cap bottles. Sterilise at 15
p.s.i. At 121C for 15 minutes. Allow the medium to set in an inclined position to give the maximum
surface area. Incubate at 30C 35C for 48 hours to check for sterility. Label and store in the fridge.
ASSAY MEDIA
Vitamin B12 assay agar M 110 (Himedia) is used. Medium to be prepared as directed on B12 assay agar
bottle.
Calculation:
Sum the diameters of the zones for each dilution and calculate the assay percent potency using the
following formula.
Where,
SH Sum of standard high doses
SL Sum of standard low dose
TH Sum of test high dose
TL Sum of test low dose
(Value of a may have a positive or negative value and should be used algebraically)
Calculate the Vitamin B12 content as mcg per g.
Mcg / g = % Pot. X Std wt (mg) X Std. Pot. (as is basis) / Sample wt. X