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Step 1: Make an INCUBATOR to grow our Step 2: Make a Growth Media for our microbes
bacteria in a controlled environment to grow on
Method: We used gloves and brand new Method: 1. Add cup chicken broth, 1 teaspoon sugar
and 3 packets of gelatin to glass dish, Stir
foil, lined a box with the foil to create a Materials
Chicken Stock
quickly, microwave for 50 seconds on high
2. Add another cup of chicken broth to
sealed environment for our media plates White Sugar mixture and stir vigorously
to hang out and grow Plain Gelatin
Petri Dishes w/ lids
3. Pour the mixture into petri dishes
4. Place loose lid over dishes while they
Glass Dish harden
Stirring Spoon 5. Once mixture has solidified store in
Gloves refrigerator in a sealed plastic bag,
inverted.
Learning Objective: To find microbes and growth them into visible colonies that can be seen with the naked eye
Step 3: Inoculate our growth media plates with Step 4: Incubate our inoculated plates
microbes
Method:
1. Label growth media plates with specimen collection site and date.
2. Use sterile cotton swabs, dip cotton swab in fresh water, rub on
any surface where you think you can collect microbes.
3. Inoculate the growth media plates by gently running swab over the
surface of the growth media.
Learning Objective: To find microbes and growth them into visible colonies that can be seen with the naked eye
Step 5: Track our microbial colony growth
Is that why I am
not allowed on
the couch?
What?!?!
Learning Objective: To identify characteristics of microbial colonies, and be able to distinguish differences in microbial colonies
Vocabulary:
Prokaryotic Cell
Bacteria
Concepts to Understand:
Coccus
- Difference between prokaryotic and Eukaryotic Cells
Bacilli
- Bacterial Cell Structure
Coccobacilli
- Identification of Bacterial cell shapes
Inoculation
- Identification of microbial colonies and how to describe them
Colony Morphology
using colony morphology
Decomposers
- How to collect and grow microbial colonies
Virus
- Bacteria versus a Virus
Bacteriophage
- A specific Bacterial infection
Microscopic
- A specific Viral Infection