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EXPERIMENT 2:
SEPARATION OF ALCOHOLS USING GAS
CHROMATOGRAPHY
OBJECTIVE:
INSTRUMENTATION:
mixture. The time required for the peak to appear, the retention time, can be used to
identify the components. Areas under the peaks can be used to measure the relative
APPARATUS:
ALCOHOLS USED:
PROCEDURE:
Warm up the GC (see the operating manual for the instruments) and read the instructions for
CAUTION: The gas chromatograph is like an oven, so the port may be hot.
1. Inject methanol using auto sampling injection and start the data collection.
CDB 3093 Analytical Chemistry Laboratory Sep 2017 P age |3
2. When the peak in the chromatogram returns to baseline, the run may be stopped by
3. After the peak is obtained, record data and print the graph as instructed.
2. Repeat steps above used for the known alcohol samples. When running the unknown
alcohol mixture sample, be certain to let the data collect long enough to get all possible
3. Identify the alcohols present by comparing the retention time of the known alcohols.
total area
CDB 3093 Analytical Chemistry Laboratory Sep 2017 P age |4
RESULTS SHEET
Starting temperature
Holding time
Ramping rate
Final temperature
Methanol
Ethanol
Peak 1
Peak 2
Total
CDB 3093 Analytical Chemistry Laboratory Sep 2017 P age |5
QUESTIONS:
3. If a known sample is properly injected into the gas chromatograph and several peaks are
4. Why is it important to clean the syringe before inject another new sample?