MASTITIS VACUNA

The microbial agents used for prevention and treatment of mastitis cows to healthy cows, is to soak in after
each milking to wash, or spray method of treating cow's mammary gland, if soaking rinse time Imin; if
spraying the bovine mammary evenly sprayed twice, to do not stay dead; after milking bdellovibrio soaking
or spraying timely breast nipple hole opened in the closed state, prevent the entry of bacteria; which
bdellovibrio quality leech body concentration l (fpfu / ml at least, it is preferably in the range of IO5 ~l (fpfu /
ml;
[0026] The microbial formulations for prevention and treatment of mastitis, may be the microbial
preparation process dairy farming environment, dairy feed and drinking water;
[0027] The microbial formulation is sprayed manner of dairy farming environment, including the barn, the
ground and the cattle manure ditch bed; wherein the concentration of bdellovibrio leech plastids at least
102pfu / ml, which is preferably in the range of IO3~ 106pfu / ml;
[0028] The microbial agents added to feed for dairy cows and cows in drinking water; concentration
bdellovibrio leech plastid should be greater than 102pfu / ml; directly used for animal feed, its use amount
of at least 15ml / kg, preferably use range of 20~35ml / kg; used in drinking water, the amount used at least
100ml / m3.

Preparation of mastitis prevention and control of microbial agents [0045] (2)

[0046] 2 respectively IOOmL LB broth containing Erlenmeyer flask was inoculated Escherichia coli
(purchased from Guangdong Province Culture Collection Center, No. GIM 1. 137), 200rpmJ8 C shaker for 18
hours culture liquid were 4 C, 5000rpm centrifuge 15min, the supernatant was discarded. The precipitate
were added to two IOOmL DNB containing liquid medium (nutrient broth 0. 8g, casein acid hydrolyzate 0.
5g, yeast extract extract 0. Ig, IOOOml dissolved in distilled water, pH value of 7. 2~7. 6) of the conical flask,
then were added to the plate were picked up from the bunk of bdellovibrio BDSOl plaques and bdellovibrio
BDS02 plaques. 250rpmJ8 C thermostat shaker culture 36h. Broth were centrifuged at 4 C 6000rpm
20min, removal containing supernatant bdellovibrio swimming body to retain precipitation (ie bdellovibrio
leech plastid), added DNB liquid medium (nutrient broth 0. Sg, caseinates hydrolyzate 0. 5g, yeast extract
extract 0. lg, IOOOml dissolved in distilled water, pH value of 7. 2~7. 6) were resuspended bdellovibrio leech
plastid. [0047] A microbial preparation having a concentration of 102pfu / bdellovibrio BDSOl leech plastid
and concentrations ml of 102pfu / ml of bdellovibrio BDS02 leech plastid by bacterial counts 1: 1 mixture of
components.
[0048] Microorganisms B by the concentration of 103pfu / bdellovibrio BDSOl leech plastid and
concentrations ml of l (fpfu / ml of bdellovibrio BDS02 leech plastid by bacterial counts 1: 1 mixture of
components.
he prevention and treatment of mastitis prophylactic effect of microbial agents mastitis occurrence:
[0050] 100 cows as a group experiment.
[0051] a set of step (1) A disinfection microbial agents prepared culture environment, spray twice a month.
A control group, the control group (also composed by the 100 cows) disinfected with conventional
disinfectants (mass percentage 4% cresol soap), disinfection twice a month. A month later observed
incidence of mastitis.
When the [0052] a set of step (1) A disinfection microbial agents prepared culture environment and were
used before and after each milking step Microorganisms B (1) Preparation of uniform spraying breast twice,
environmental disinfection of aquaculture month spray twice. A control group, the control group (also
composed by the 100 cows) with conventional disinfectants (mass percentage 4% cresol soap) disinfection
disinfection breeding environment twice a month, and the average percentage before and after milking
each with volume 75 % alcohol wipe breast. A month later observed incidence of mastitis.
[0053] One group was treated with microbial agents disinfect breeding environment, feed, drinking water
and breast: prepared in Step A microbial preparation for when environmental disinfection, spray twice a
month; the cows fed with the step before 24h
[0054] (1) A microbial agents were prepared in accordance with 30ml / kg of the amount added to the feed
as well as by the amount of 10ml / 100ml was added to the drinking water used in dairy cattle; both before
and after each milking step (1) Preparation of Microorganisms B evenly sprayed breast twice. Set in the
control group (which is also composed of the 100 cows), disinfection by conventional breeding environment
disinfectant (mass percentage 4% cresol soap) disinfection twice a month; 24h before feeding cows by
adding antibiotics bacitracin zinc additive in animal feed ( Product Name: Alpharma Yakult prime, raw
powder, Ikg / bag, veterinary raw materials purchased in Jinan Xinyuan Ltd.) IOOOg / tonne and by 10mg /
100ml adding chlorine dioxide disinfectant in drinking water; before and after each milking the percentage
of 75% by volume of alcohol wipe breast. A month later observed incidence of mastitis.