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Nucleic Acid
Nucleic acids made up of chains of nucleotides
Nucleotides consist of:
o A base
o A sugar (ribose)
o A phosphate
The nitrogenous bases of each nucleotide are classified as a purine or pyrimidine based on their chemical
structure. A purine is a double-ringed structure, whereas a pyrimidine is a single-ringed structure. The
nitrogenous bases adenine and guanine are purines; cytosine and thymine (DNA) or uracil (RNA) are pyrimidines.
Two types of nucleic acids in cells:
o Deoxyribonucleic acid (DNA)
o Ribonucleic acid (RNA)
Features of DNA and RNA
DNA RNA
Mainly concentrated in the nucleus Mainly concentrated in the cytoplasm although
Location
it is also occurs in the nucleus
Replication of pre-existing DNA Usually synthesized on a DNA template; only
Origin genetic RNA may replicate to yield RNA
DNA REPLICATION
During normal cell division, it is essential that all components of the cell,
including the chromosomes, replicate so that each progeny cell receives a
copy of the chromosomes from the parent cell. The process of replicating
DNA must happen accurately to ensure that no changes to the DNA are
passed on to the progeny cells.
The process of DNA replication is termed semiconservative replication.
One double helix must be replicated so that two double helices resultone
for each progeny cell. Because the DNA double helix has two strands, each
strand can serve as a template to produce a new strand.
Elongation: RNA polymerase elongates the nascent RNA molecule in a 5-to-3 direction, antiparallel to the
template DNA
o Nucleotides are added by complementary base pairing with the template strand
o The substrates, ribonucleoside triphosphates, are hydrolyzed as added, releasing energy for RNA
synthesis.
Termination: Special DNA sequences and protein helpers terminate transcription.
o The transcript is released from the DNA.
o This Primary Transcript is called the pre-mRNA
o The pre-mRNA is processed to generate the mature mRNA
The Genetic Code
Describes how nucleotide sequence is converted to protein sequence
Unit of three nucleotides = a codon
A codon codes for a specific amino acid (structural component of protein)
There are 64 variations of codons
The start codon, AUG, codes for methionine- starts translation
The stop codons UAA, UGA, UAG stop translation
Four RNA nucleotides are arranged 20 different ways to make 20 different amino acids building blocks of
proteins
Characteristics of Code
o Continuity - The genetic code reads as a long series of three-letter codons that have no spaces or
punctuation and never overlap.
o Redundancy Several different codons can code for the same amino acid, but no codon ever has more
than one amino acid counterpart.
o Universality the genetic code is the same in almost all living organisms, from bacteria to mamma
Ribosomes
Small organelles that are made up of rRNA and proteins.
Composed of two subunits (small and large)
Located on the rough ER or in the cytoplasm. There are also a few
ribosomes in mitochondria and chloroplast
Function: makes proteins by reading the code on the mRNA molecule
Binding sites:
o A-site binds to the tRNA anticodon that carries the proper amino acid
o P-site binds to the last entering tRNA that holds the growing polypeptide chain
o E-site allows the exit of the empty tRNA
2. Translation Protein Synthesis
the synthesis of protein from an RNA template
It takes place in ribosomes in the cytoplasm or on the surface of the endoplasmic reticulum
The product of this process is a polypeptide chain (primary protein structure)
The actual process is composed of an initiation, elongation, and termination stage.
During initiation all participants of translation come together: mRNA (for genetic information) bind to the small
subunit of the ribosome. The tRNA molecule that carries Met (start codon) also attaches to the small subunit
(on the A-site). Finally, the large subunit binds to the small subunit. The copying starts at the 5 end of the
mRNA. This process requires energy (GTP or Guanosine triphosphate provides it)
During elongation: The ribosome moves over and reads the next codon on the mRNA. The matching tRNA
enters to the A-site, the previous tRNA moves over to the P-site. The amino acids on the two tRNA molecules
bind by peptide bond. This process keeps repeating until a stop signal on the mRNA is reached.
Termination starts when the stop codon on the mRNA is reached. Instead of the tRNA, a release factor comes
in, reads the triplet and makes the entire complex of mRNA, tRNA, polypeptide fall apart.